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Change of retinal nerve fiber layer thickness in patients with nonarteritic inflammatory anterior ischemic optic neuropathy

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Neural Regeneration Research 2012年第35期7卷 2778-2783页

作者： Tingting liu Hongsheng Bi xingrong Wang Guimin Wang Haiyan li Hui Wu Yi Qu Ying Wen Chenyang Cong Daoguang Wang Eye Institute of Shandong Traditional Chinese Medicine University Affiliated Eye Hospital of Shandong University of Traditional Chinese Medicine Ophthalmology Department of Second Affiliated Hospital of Shandong University of Traditional Chinese Medicine

In this study, 16 patients （19 eyes） with nonarteritic anterior ischemic optic neuropathy in the acute stage （within 4 weeks） and resolving stage （after 12 weeks） were diagnosed by a series of complete ophthalmic examinations, including fundus examination, optical coherence tomography and fluorescein fundus angiography, and visual field defects were measured with standard automated perimetry. The contralateral uninvolved eyes were used as controls. The retinal nerve fiber layer thickness was determined by optical coherence tomography which showed that the mean retinal nerve fiber layer thickness and the retinal nerve fiber layer thickness from temporal, superior, nasal and inferior quadrants were significantly higher for all measurements in the acute stage than the corresponding normal values. In comparison, the retinal nerve fiber layer thickness from each optic disc quadrant was found to be significantly lower when measured at the resolving stages, than in the control group. Statistical analysis on the correlation between optic disc nerve fiber layer thickness and visual defects demonstrated a positive correlation in the acute stage and a negative correlation in the resolving stage. Our experimental findings indicate that optical coherence tomography is a useful diagnostic method for nonarteritic anterior ischemic optic neuropathy and can be used to evaluate the effect of treatment.

关键词： nonarteritic anterior ischemic optic neuropathy optical coherence tomography retinal nerve fiberlayer visual field optic nerve peripheral nerve injury neural regeneration

基于网络药理学和分子对接技术探讨十九畏药对“郁金-丁香”抗肝纤维化作用机制

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安徽医药 2023年第6期27卷 1083-1087,F0002,I0001页

作者：汪居安王居义蔡叶李幸蓉吴凌伊艳敏檀啸王继洲中国科学技术大学附属第一医院肝胆外科安徽合肥241002 皖南医学院临床医学院安徽芜湖230001 安徽中医药大学中医学院安徽合肥230038 安徽理工大学医学院安徽淮南232001

目的通过网络药理学和分子对接技术探讨郁金-丁香药对抗肝纤维化的作用机制。方法于2022年1月8日检索中药系统药理学数据平台(TCMSP)获取郁金和丁香的有效活性成分及相关靶点并进行筛选和预测;肝纤维化相关靶点的数据通过NCBI基因数据... 详细信息

目的通过网络药理学和分子对接技术探讨郁金-丁香药对抗肝纤维化的作用机制。方法于2022年1月8日检索中药系统药理学数据平台(TCMSP)获取郁金和丁香的有效活性成分及相关靶点并进行筛选和预测;肝纤维化相关靶点的数据通过NCBI基因数据、OMIM数据库和Genecards数据库获取;运用软件Venny 2.1绘制药物-疾病靶点韦恩图,得到药物抗肝纤维化的作用靶点;运用Cytoscape 3.8.2软件构建药物-活性成分-靶点相互作用网络并分析其拓扑结构;运用String数据库构建蛋白质相互作用(PPI)网络图;利用David和Metascape数据库对关键靶点分别进行基因本体(GO)和京都基因与基因组百科全书(KEGG)富集分析;利用PubChem和RCSBPDB数据库对关键成分和靶点进行分子对接。结果筛选得到郁金-丁香药活性成分44个,对应作用靶点200个,肝纤维化候选靶点3 951个,药物与疾病交集靶点155个。药物-活性成分-靶点网络和PPI网络分析显示槲皮素、山柰酚、柚皮素、β-谷甾醇等是药对治疗肝纤维化的关键成分,蛋白激酶B1(AKT1)、白细胞介素(IL)-6、IL-1β、牛胱天蛋白酶3(CASP3)等为治疗的核心靶点。155个潜在靶点经GO富集至1 889条生物学过程、44条细胞组分表达和94个分子功能相关的过程中。KEGG通路分析显示与133条通路有关,涉及癌症信号通路、乙型肝炎信号通路、TNF信号通路、Toll样受体信号通路等。分子对接表明关键成分与核心靶点结合良好。结论从网络药理学角度阐明了郁金-丁香药多成分、多靶点、多途径的整体调节特点,初步揭示了其抗肝纤维化的作用机制,为后续研究提供思路与依据。

关键词：肝纤维化十九畏郁金丁香网络药理学分子对接蛋白质相互作用图谱

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Orange-red, green, and blue fiuorescence carbon dots for white light emitting diodes

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Journal of Materials Science & Technology 2020年第15期47卷 184-191页

作者： ling Jin li Zhang Lei Yang xingrong Wu Cheng Zhang Kang Wei lifang He Xinya Han Hongbin Qiao Abdullah MAsiri Khalid AAlamry Kui Zhang School of Chemistry and Chemical Engineering Anhui University of TechnologyMa'anshan243032China Department of Chemistry University of North Carolina at Chapel HillChapel HillNC27599USA Key Laboratory of Metallurgical Emission Reduction&Resources Recycling Ministry of Education(Anhui University of Technology)Ma'anshan243002China Chemistry Department Faculty of ScienceKing Abdulaziz UniversityJeddah21589Saudi Arabia

Fluorescent carbon dots(CDs) are admirable nanomaterials due to their superior optical properties and low ***,regulation of the emission colors in CDs for lighting device still remains a *** this work,we developed a green hydrothermal method to obtain three emission colors of CDs using L-tyrosine(for blue CDs),o-phenylendiamine(for green CDs) and L-tyrosine/o-phenylendiamine mixture(for orange-red CDs).These CDs possess good water dispersibility,strong emission with high quantum yields,and excellent ***,the resulting CDs were dispersed in polyvinyl alcohol(PVA) matrix to yield solid state films,where the self-quenching effect in solid state was effectively avoided.A full-color light-emitting diode was fabricated by packing the CDs-PVA composites on the top of a UV-chip,which have superior potential applications in CDs-based solid-state lighting system.

关键词： Fluorescence Photoluminescene Carbon dots Self-quenching effect light-emitting diode

Differentiation of neuron-like cells from mouse parthenogenetic embryonic stem cells

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Neural Regeneration Research 2013年第4期8卷 293-300页

作者： xingrong Yan Yanhong Yang Wei liu Wenxin Geng Huichong Du Jihong Cui Xin Xie Jinlian Hua Shumin Yu liwen li Fulin Chen College of life Sciences Northwest University Department of Obstetrics & Gynecology Tangdu Hospitalthe Fourth Military Medical University of Chinese PLA College of Veterinary Medicine Northwest A&F University College of Veterinary Medicine Sichuan Agricultural University

Parthenogenetic embryonic stem cells have pluripotent differentiation potentials, akin to fertilized embryo-derived embryonic stem cells. The aim of this study was to compare the neuronal differentiation potential of parthenogenetic and fertilized embryo-derived embryonic stem cells. Before differentiation, karyotype analysis was performed, with normal karyotypes detected in both parthenogenetic and fertilized embryo-derived embryonic stem cells. Sex chromosomes were identified as XX. Immunocytochemistry and quantitative real-time PCR detected high expression of the pluripotent gene, Oct4, at both the mRNA and protein levels, indicating pluripotent differentiation potential of the two embryonic stem cell subtypes. Embryonic stern cells were induced with retinoic acid to form embryoid bodies, and then dispersed into single cells. Single cells were differentiated in N2 differentiation medium for 9 days. Immunocytochemistry showed parthenogenetic and fertilized embryo-derived embryonic stem cells both express the neuronal cell markers nestin, ~lll-tubulin and myelin basic protein. Quantitative real-time PCR found expression of neuregenesis related genes （Sox-1, Nestin, GABA, Pax6, Zic5 and Pitxl） in both types of embryonic stem cells, and Oct4 expression was significantly decreased. Nestin and Pax6 expression in parthenogenetic embryonic stem cells was significantly higher than that in fertilized embryo-derived embryonic stem cells. Thus, our experimental findings indicate that parthenogenetic embryonic stem cells have stronger neuronal differentiation potential than fertilized embryo-derived embryonic stem cells.

关键词： neural regeneration stem cells parthenogenesis parthenogenetic embryonic stem cells embryonic stem cells neuronal cells karyotypes Oct4 differentiation embryoid body mice grants-supported paper photographs-containing paper neuroregeneration

Optimization of Determination of Sucralose in Drink by HPLC

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Journal of Economic Science Research 2019年第3期2卷 50-54页

作者： Sang li xingrong Chen Guangdong Institute of Food Inspection(Guangdong Inspection Center of Wine) Guangzhou510435China

To optimize the method for determination of Sucralose in drink by high performance liquid chromatography(HPLC).Using HPLC with RID,operating conditions were C18 reversed phase chromatograph column,40:60=Methanol:0.125%K2HPO4 as mobil phase,measured at a flow rate of 0.8 mL/*** the range of 20~400 mg L,with the concentration of Sucralose and corresponding peak area as standard,r=0.9999,it has good correlation,the recovery of sucralose is 94~108%.The lower limit of detection of Sucralose was 0.0024 g/*** method not only meets the requirements of national standards,but also fast,sensitive,and environmentally friendly,improves the detection efficiency and safety of the detection of sucralose in drink by high performance liquid chromatography.

关键词： Drink Sucralose HPLC RID

Will the Historic Southeasterly Wind over the Equatorial Pacific in March 2022 Trigger a Third-year La Niña Event?

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Advances in Atmospheric Sciences 2023年第1期40卷 6-13页

作者： Xianghui FANG Fei ZHENG Kexin li Zeng-Zhen HU Hongli REN Jie WU xingrong CHEN Weiren LAN Yuan YUAN licheng FENG Qifa CAI Jiang ZHU Department of Atmospheric and Oceanic Sciences&Institute of Atmospheric Sciences Fudan UniversityShanghai 200438China International Center for Climate and Environment Science(ICCES) Institute of Atmospheric PhysicsChinese Academy of SciencesBeijing 100029China Collaborative Innovation Center on Forecast and Evaluation of Meteorological Disasters Nanjing University of Information Science&TechnologyNanjing 210044China Climate Prediction Center NCEP/NWS/NOAACollege ParkMD 20740USA State Key Laboratory of Severe Weather Chinese Academy of Meteorological SciencesBeijing 100081China National Climate Center Beijing 100081China National Marine Environmental Forecasting Center Beijing 100081China Mailbox 5111 Beijing 100094China

Based on the updates of the Climate Prediction Center and International Research Institute for Climate and Society(CPC/IRI)and the China Multi-Model Ensemble(CMME)El Niño-Southern Oscillation(ENSO)Outlook issued in April 2022,La Niña is favored to continue through the boreal summer and fall,indicating a high possibility of a three-year La Niña(2020-23).It would be the first three-year La Niña since the 1998-2001 event,which is the only observed three-year La Niña event since *** examining the status of air-sea fields over the tropical Pacific in March 2022,it can be seen that while the thermocline depths were near average,the southeasterly wind stress was at its strongest since ***,based on a quaternary linear regression model that includes various relevant air-sea variables over the equatorial Pacific in March,we argue that the historic southeasterly winds over the equatorial Pacific are favorable for the emergence of the third-year La Niña,and both the anomalous easterly and southerly wind stress components are important and contribute~50%of the third-year La Niña growth,***,the possible global climate impacts of this event are discussed.

关键词： El Niño-Southern Oscillation three-year La Niña strongest southeasterly wind air-sea interaction

QTL mapping of drought tolerance traits in soybean with SLAF sequencing

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The Crop Journal 2020年第6期8卷 977-989页

作者： Honglei Ren Jianan Han xingrong Wang Bo Zhang lili Yu Huawei Gao Huilong Hong Rujian Sun Yu Tian Xusheng Qi Zhangxiong liu Xiaoxia Wu li-Juan Qiu College of Agriculture Northeast Agricultural UniversityHarbin 150030HeilongjiangChina National Key Facility for Crop Gene Resources and Genetic Improvement Institute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Institute of Crop Sciences Gansu Academy of Agricultural SciencesLanzhou 730070GansuChina School of Plant and Environmental Sciences Virginia Polytechnic Institute and State UniversityBlacksburgVA 24060USA Heilongjiang Academy of Agricultural Sciences Harbin 150086HeilongjiangChina

Drought stress is an important factor affecting soybean *** drought tolerance of soybean varieties can increase yield and yield stability when the stress *** QTL related to drought tolerance using molecular marker-assisted selection is able to facilitate the development of drought-tolerant soybean *** this study,we used a high-yielding and drought-sensitive cultivar‘Zhonghuang 35’and a drought-tolerant cultivar‘Jindou 21’to establish F6:9 recombinant inbred *** constructed a highdensity genetic map using specific locus amplified fragment sequencing(SLAF-Seq)*** genetic map contained 8078 SLAF markers distributing across 20 soybean chromosomes with a total genetic distance of 3780.98 c M and an average genetic distance of0.59 c M between adjacent *** treatments(irrigation and drought)were used in the field tests,the Additive-Inclusive Composite Interval Mapping(ICIM-ADD)was used to call QTL,and plant height and seed weight per plant were used as the indicators of drought *** identified a total of 23 QTL related to drought *** them,seven QTL(q PH2,q PH6,q PH7,q PH17,q PH19-1,q PH19-2,and q PH19-3)on chromosomes 2,6,7,17,and 19 were related to plant height,and five QTL(q SWPP2,q SWPP6,q SWPP13,q SWPP17,and q SWPP19)on chromosomes 2,6,13,17,and 19 were related to seed weight and could be considered as the major *** addition,three common QTL(q PH6/q SWPP6,q PH17/q SWPP17,and q PH19-3/q SWPP19)for both plant height and seed weight per plant were located in the same genomic regions on the same ***(q PH2,q PH17,and q PH19-2)and four novel QTL(q SWPP2,q SWPP13,q SWPP17,and q SWPP19)were identified for plant height and seed weight per plant,*** pairs of QTL(q PH2/q SWPP2 and q PH17/q SWPP17)were also common for both plant height and seed weight per *** QTL and closely linked SLAF markers could be used to accelerate breeding for drought tolerant cultivars via

关键词： Soybean drought tolerance Simplified genome sequencing Quantitative trait loci Plant height Seed weight per plant

Epoxidation of Styrene-Isoprene-Styrene Block Copolymer and Research on Its Reaction Mechanism

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Journal of Wuhan University of Technology(Materials Science) 2010年第3期25卷 403-407页

作者：李红强曾幸荣 College of Materials Science and Engineering South China University of Technology

Styrene-isoprene-styrene（SIS） block copolymer was modified into epoxidized styrene-isoprene-styrene（ESIS） block copolymer with performic acid generated in situ from hydrogen peroxide and formic *** structure and property of ESIS were characterized by Fourier transform infrared（FT-IR） spectroscopy,gel permeation chromatography（GPC）,thermogravimetric/differential thermogravimetric（TG/DTG）,melt flow rate（MFR） and dynamic mechanical analysis（DMA）,and the reaction mechanism in the process of epoxidation was *** results showed that C=C double bonds of 1,4-structure were more active than that of 3,4-structure in polyisoprene *** epoxidation reaction proceeding,the whole tendency of molecular weight increased and molecular weight distribution widened,and MFR firstly increased and latterly *** heat resistance of ESIS was superior to that of *** SIS was changed into ESIS with 15.3% of mass fraction of epoxide groups,Tg of polyisoprene chains increased from-45.3 ℃ to 10.9 ℃.In the earlier period of epoxidation,some molecular chains ruptured and new substances with low molecular weight ***,in the latter period,crosslinking reaction between molecular chains which was initiated by epoxide groups or C=C double bonds occurred and crosslinked insoluble substances came into being.

关键词： styrene-isoprene-styrene block copolymer epoxidation ring-open reaction mechanism

Recent advances in the translation of drug metabolism and pharmacokinetics science for drug discovery and development

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Acta Pharmaceutica Sinica B 2022年第6期12卷 2751-2777页

作者： Yurong Lai Xiaoyan Chu li Di Wei Gao Yingying Guo xingrong liu Chuang Lu Jialin Mao Hong Shen Huaping Tang Cindy Q.Xia Lei Zhang Xinxin Ding Drug Metabolism Gilead Sciences Inc.Foster CityCA 94404USA Department of Pharmacokinetics Pharmacodynamics and Drug MetabolismMerck&Co.Inc.KenilworthNJ 07033USA Pharmacokinetics Dynamics and MetabolismPﬁzer Worldwide Research and DevelopmentGrotonCT 06340USA Eli lilly and Company IndianapolisIN 46221USA Drug Metabolism and Pharmacokinetics BiogenCambridgeMA 02142USA Drug Metabolism and Pharmacokinetics Accent TherapeuticsInc.LexingtonMA 02421USA Department of Drug Metabolism and Pharmacokinetics GenentechA Member of the Roche GroupSouth San FranciscoCA 94080USA Drug Metabolism and Pharmacokinetics Department Bristol-Myers Squibb CompanyPrincetonNJ 08540USA Bioanalysis and Biomarkers Glaxo Smith KlineKing of the PrussiaPA 19406USA Department of Drug Metabolism and Pharmacokinetics Takeda Pharmaceuticals International Co.CambridgeMA 02139USA Ofﬁce of Research and Standards Ofﬁce of Generic DrugsCDERFDASilver SpringMD 20993USA Department of Pharmacology and Toxicology College of PharmacyUniversity of ArizonaTucsonAZ 85721USA Current afﬁliation:Translational Medicine EMD Serono Research&Development InstituteInc.BillericaMA 01821USA Current afﬁliation:Drug Metabolism Gilead Sciences Inc.Foster CityCA 94404USA

Drug metabolism and pharmacokinetics(DMPK) is an important branch of pharmaceutical *** nature of ADME(absorption,distribution,metabolism,excretion) and PK(pharmacokinetics) inquiries during drug discovery and development has evolved in recent years from being largely descriptive to seeking a more quantitative and mechanistic understanding of the fate of drug candidates in biological *** progress has been made in the past decade,not only in the characterization of physiochemical properties of drugs that influence their ADME,target organ exposure,and toxicity,but also in the identification of design principles that can minimize drug-drug interaction(DDI) potentials and reduce the *** importance of membrane transporters in drug disposition,efficacy,and safety,as well as the interplay with metabolic processes,has been increasingly *** increases in investments on new modalities beyond traditional small and large molecule drugs,such as peptides,oligonucleotides,and antibody-drug conjugates,necessitated further innovations in bioanalytical and experimental tools for the characterization of their ADME *** this review,we highlight some of the most notable advances in the last decade,and provide future perspectives on potential major breakthroughs and innovations in the translation of DMPK science in various stages of drug discovery and development.

关键词： Drug discovery and development New drug application Biologics license application Pharmacokinetics ADME New modalities Model-informed drug development Micro-physiological systems