QTL mapping of drought tolerance traits in soybean with SLAF sequencing

作     者：Honglei Ren Jianan Han Xingrong Wang Bo Zhang Lili Yu Huawei Gao Huilong Hong Rujian Sun Yu Tian Xusheng Qi Zhangxiong Liu Xiaoxia Wu Li-Juan Qiu Honglei Ren;Jianan Han;Xingrong Wang;Bo Zhang;Lili Yu;Huawei Gao;Huilong Hong;Rujian Sun;Yu Tian;Xusheng Qi;Zhangxiong Liu;Xiaoxia Wu;Li-Juan Qiu

作者机构：College of AgricultureNortheast Agricultural UniversityHarbin 150030HeilongjiangChina National Key Facility for Crop Gene Resources and Genetic ImprovementInstitute of Crop SciencesChinese Academy of Agricultural SciencesBeijing 100081China Institute of Crop SciencesGansu Academy of Agricultural SciencesLanzhou 730070GansuChina School of Plant and Environmental SciencesVirginia Polytechnic Institute and State UniversityBlacksburgVA 24060USA Heilongjiang Academy of Agricultural SciencesHarbin 150086HeilongjiangChina 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2020年第8卷第6期

页      面：977-989页

核心收录：

学科分类：09[农学] 0901[农学-作物学] 

基　　金：supported by the National Key Research and Development Program of China(2016YFD0100201 and 2016YFD0100304) the National Science and Technological Innovation Program of China 

主　　题：Soybean drought tolerance Simplified genome sequencing Quantitative trait loci Plant height Seed weight per plant 

摘      要：Drought stress is an important factor affecting soybean *** drought tolerance of soybean varieties can increase yield and yield stability when the stress *** QTL related to drought tolerance using molecular marker-assisted selection is able to facilitate the development of drought-tolerant soybean *** this study,we used a high-yielding and drought-sensitive cultivar‘Zhonghuang 35’and a drought-tolerant cultivar‘Jindou 21’to establish F6:9 recombinant inbred *** constructed a highdensity genetic map using specific locus amplified fragment sequencing(SLAF-Seq)*** genetic map contained 8078 SLAF markers distributing across 20 soybean chromosomes with a total genetic distance of 3780.98 c M and an average genetic distance of0.59 c M between adjacent *** treatments(irrigation and drought)were used in the field tests,the Additive-Inclusive Composite Interval Mapping(ICIM-ADD)was used to call QTL,and plant height and seed weight per plant were used as the indicators of drought *** identified a total of 23 QTL related to drought *** them,seven QTL(q PH2,q PH6,q PH7,q PH17,q PH19-1,q PH19-2,and q PH19-3)on chromosomes 2,6,7,17,and 19 were related to plant height,and five QTL(q SWPP2,q SWPP6,q SWPP13,q SWPP17,and q SWPP19)on chromosomes 2,6,13,17,and 19 were related to seed weight and could be considered as the major *** addition,three common QTL(q PH6/q SWPP6,q PH17/q SWPP17,and q PH19-3/q SWPP19)for both plant height and seed weight per plant were located in the same genomic regions on the same ***(q PH2,q PH17,and q PH19-2)and four novel QTL(q SWPP2,q SWPP13,q SWPP17,and q SWPP19)were identified for plant height and seed weight per plant,*** pairs of QTL(q PH2/q SWPP2 and q PH17/q SWPP17)were also common for both plant height and seed weight per *** QTL and closely linked SLAF markers could be used to accelerate breeding for drought tolerant cultivars via