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Activation of natural killer T cells contributes to Th1 bias in the murine liver after 14 d of ethinylestradiol exposure

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World Journal of Gastroenterology 2022年第26期28卷 3150-3163页

作者： Meng-zhi Zou Wei-Chao Kong Heng Cai Meng-Tao xing Zi-Xun Yu xin Chen Lu-Yong Zhang xin-zhi wang New Drug Screening Center China Pharmaceutical UniversityNanjing 210009Jiangsu ProvinceChina Department of Pharmacology China Pharmaceutical UniversityNanjing 210009Jiangsu ProvinceChina Center for Drug Research and Development Guangdong Pharmaceutical UniversityGuangzhou 510006Guangdong ProvinceChina

BACKGROUND As the main component of oral contraceptives(OCs),ethinylestradiol(EE)has been widely applied as a model drug to induce murine intrahepatic *** clinical counterpart of EE-induced cholestasis includes women who are taking OCs,sex hormone replacement therapy,and susceptible pregnant *** intrahepatic cholestasis of pregnancy(ICP)as an example,ICP consumes the medical system due to its high-risk fetal burden and the impotency of ursodeoxycholic acid in reducing adverse perinatal *** To explore the mechanisms and therapeutic strategies of EE-induced cholestasis based on the liver immune *** Male C57BL/6J mice or invariant natural killer T(iNKT)cell deficiency(Jα18-/-mice)were administered with EE(10 mg/kg,subcutaneous)for 14 *** Both Th1 and Th2 cytokines produced by NKT cells increased in the liver skewing toward a Th1 *** expression of the chemokine/chemokine receptor Cxcr6/Cxcl16,toll-like receptors,Ras/Rad,and PI3K/Bad signaling was upregulated after EE *** also influenced bile acid synthase Cyp7a1,Cyp8b1,and tight junctions ZO-1 and Occludin,which might be associated with EEinduced *** cell deficiency(Jα18-/-mice)robustly alleviated cholestatic liver damage and lowered the expression of the abovementioned signaling *** Hepatic NKT cells play a pathogenic role in EE-induced intrahepatic *** research improves the understanding of intrahepatic cholestasis by revealing the hepatic immune microenvironment and also provides a potential clinical treatment by regulating iNKT cells.

关键词： Natural killer T cell Th1/Th2 IFN-γ Estrogen Cholestasis

Preparation and Characterization of Phosphorus Pentafluoride Gas for Lithium Ion Battery Electrolyte Salt

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Chemical Research in Chinese Universities 2009年第6期25卷 791-795页

作者： LIU Jian-wen LI xin-hai wang zhi-xing GUO Hua-jun HU Qi-yang School of Metallurgical Science and Engineering Central South University Changsha 410083 P. R. China

A novel preparation method of phosphorus pentafluoride（PF5） is introduced in details in the article. Phosphorus pentafluoride is mainly used to synthesize lithium ion battery electrolyte salt. In the experiment mesneproduct- hexafluorophosphoric acid（HPF6） was firstly prepared-from phosphorus pentoxide（P2O5） and anhydrous hydrogen fluoride（HF）, according to the chemical stoichiometrics, the optimum amount of excess HF used was about 60% and the optimum reaction time was 4 h; then HPF6 was dehydrated by oleum with an excess amount of 35%-- 40% which was heated for 40--50 min at 150 ℃; thus HPF6 was decomposed to a mixture of gases containing PF5; impurity gases were removed through condensation in the presence of cooling water at 0 ℃, then PF5 of high purity was eventually prepared. The production was characterized by inffared（IR） spectrometry and gas chromatography-mass spectroscopy-selected ion monitoring（GC-MS-SIM）. Eventual results indicate that PF5 prepared by the novel method has a high target purity and a low amount of impurity and water.

关键词： Hexafluorophosphoric acid Phosphorus pentafluoride Preparation lnfrared spectrum Gas chromatogra- phy-mass spectroscopy-selected ion monitoring

A New Route for Preparation of β-TCP/ PLLA Composite

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Chinese Chemical Letters 2006年第6期17卷 833-836页

作者： Feng ZHANG Fang MEI xin zhi wang Xiao Yang HU Yong Ming LUO Xu Liang DENG School and Hospital of Stomatology Peking University Beijing 100081 Department of Histology and Embryology Health Science Center Peking University Beijing 100083 Department of Environmental Science and Engineering Kunming University of Science and Technology Kunming 650093 The 2nd Hospital of Beijing Armed Police Forces Beijing 100037

A new facile route for preparation of β-TCP/PLLA composites is reported in this letter. SEM images display that β-TCP particles with average diameter of 400 nm were well bonded and distributed within the pore walls of the PLLA scaffolds. The mixture of the novel complex and human dental pulp cells was transplanted subcutaneously into the dorsal surface of a nude mouse. Two months later histological examination showed that new collagen and new dentin formed. The results revealed that the new nano β-TCP/PLLA composite combined with human pulp cells could induce dentin formation offering a new way to dental tissue engineering.

关键词： β-TCP particles β-TCP/PLLA composite dental pulp cells dental tissue engineering.

ROP11 GTPase Negatively Regulates ABA Signaling by Protecting ABI1 Phosphatase Activity from Inhibition by the ABA Receptor RCAR1/PYL9 in Arabidopsis

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Journal of Integrative Plant Biology 2012年第3期54卷 180-188页

作者： Zixing Li Zheng Li Xiang Gao Viswanathan ChinnusamyI Ray Bressant zhi-xin wang Jian-Kang Zhu Jia-Wei Wu Dong Liu MOE Key Laboratory of Bioinformatics School of Life Sciences Tsinghua University Beijing 100084 China Division of Plant Physiology Indian Agricultural Research Institute New Delhi 110012 India Department of Horticulture and Landscape Purdue University West Lafayette Indiana 47907 USA

The phytohormone abscisic acid （ABA） regulates many key processes in plants, such as seed germina- tion, seedling growth, and abiotic stress tolerance. In recent years, a minimal set of core components of a major ABA signaling pathway has been discovered. These components include a RCAR/PYR/PYL family of ABA receptors, a group of PP2C phosphatases, and three SnRK2 kinases. However, how the interactions between the receptors and their targets are regulated by other proteins remains largely unknown. In a companion paper published in this issue, we showed that ROP11, a member of the plant- specific Rho-like small GTPase family, negatively regulates multiple ABA responses in Arabidopsis. The current work demonstrated that the constitutively active ROP11 （CA-ROP11） can modulate the RCAR1/PYL9-mediated ABA signaling pathway based on reconstitution assays in Arabidopsis thaliana protoplasts. Furthermore, using luciferase complementation imaging, yeast two-hybrid assays, co- immunoprecipitation assays in Nicotiana benthamiana and bimolecular fluorescence complementation assays, we demonstrated that CA-ROP11 directly interacts with ABI1, a signaling component downstream of RCAR1/PYL9. Finally, we provided biochemical evidence that CA-ROP11 protects ABI1 phosphatase activity from inhibition by RCAR1/PYL9 and thus negatively regulates ABA signaling in plant cells. A model of how ROP11 acts to negatively regulate ABA signaling is presented.

关键词： ROP11 GTPase ABA signaling RCAR1/PYL9A ABI1 Arabidopsis.

Prognostic implications of estrogen receptor 1 and vascular endothelial growth factor A expression in primary gallbladder carcinoma

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World Journal of Gastroenterology 2015年第4期21卷 1243-1250页

作者： Ling-Qiang Zhang xin-Sen Xu Yong Wan Si-Dong Song Rui-Tao wang Wei Chen zhi-xin wang Hu-Lin Chang Ji-Chao Wei Ya-Feng Dong Chang Liu Department of Hepatobiliary Surgery the First Affiliated Hospital of Medical CollegeXi’an Jiaotong University Department of Obstetrics and Gynecology University of Kansas School of MedicineKS 66160United States

AIM: To investigate the prognostic significance of estrogen receptor 1(ER1) and vascular endothelial growth factor A(VEGF-A) expression in primary gallbladder carcinoma(GBC) to identify new prognostic markers for this ***: Using immunohistochemistry, we investigated ER1 and VEGF-A expression in 78 GBC and 78 cholelithiasis(CS) tissues. The results were correlated with clinicopathological features. Univariate and multivariate analyses were performed to evaluate the relationship between ER1 and VEGF-A expression and patients' prognosis. Further Kaplan-Meier survival analysis was also performed. RESULTS: ER1 and VEGF-A expression was significantly higher in GBC compared with CS(47/78 vs 28/78, P < 0.05; 51/78 vs 33/78, P < 0.05). ER1 expression was correlated with gender(P < 0.05) and VEGF-A expression was correlated with tumor differentiation in GBC patients(P < 0.05). In univariate analysis, age and tumor node metastasis(TNM) stage were factors associated with GBC prognosis(P < 0.05). Although there was no statistical difference between the expression of ER1 or VEGF-A and overall survival, the high expression of ER1 combined with VEGF-A predicted a poor prognosis for GBC patients(16.30 ± 1.87 vs 24.97 ± 2.09, log-rank P < 0.05). In multivariate analysis, combined expression of ER1 and VEGF-A and TNM stage were independent prognostic factors for GBC patients(P < 0.05).CONCLUSION: Combined expression of ER1 and VEGF-A is a potential prognostic marker for GBC patients. Clinical detection of ER1 and VEGF-A in surgically resected GBC tissues would provide animportant reference for decision-making of postoperative treatment programs.

关键词： Gallbladder carcinoma Estrogen receptor 1 Vascular

Polymorphisms of glutathione S-transferase genes and survival of resected hepatocellular carcinoma patients

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World Journal of Gastroenterology 2015年第14期21卷 4310-4322页

作者： Kai Qu Su-Shun Liu zhi-xin wang Zi-Chao Huang Si-Nan Liu Hu-Lin Chang xin-Sen Xu Ting Lin Ya-Feng Dong Chang Liu Department of Hepatobiliary Surgery The First Affiliated Hospital of Medical CollegeXi’an Jiaotong University Department of Obstetrics and Gynecology University of Kansas School of Medicine

AIM: To investigate the effects of single nucleotide polymorphisms(SNPs) in glutathione S-transferase(GST) genes on survival of hepatocellular carcinoma(HCC) ***: Twelve tagging SNPs in GST genes(including GSTA1, GSTA4, GSTM2, GSTM3, GSTO1, GSTO2 and GSTP1) were genotyped using Sequenom Mass ARRAY i PLEX genotyping method in a cohort of 214 Chinese patients with resected *** Cox proportional hazards model and log-rank test were performed to determine the SNPs related to ***, stratified analysis was performed at each level of the demographic and clinical *** SNP-gene expression association model was further established to investigate the correlation between SNP and gene ***: Two SNPs(GSTO2 : rs7085725 and GSTP1 : rs4147581) were significantly associated with overall survival in HCC patients(P = 0.035 and 0.042, respectively).In stratified analysis, they were more significantly associated with overall survival in patients with younger age, male gender and *** further investigated cumulative effects of these two SNPs on overall survival in HCC *** with the patients carrying no unfavorable genotypes, those carrying 2 unfavorable genotypes had a 1.70-fold increased risk of death(P < 0.001).The cumulative effects were more significant in those patients with younger age, male gender and cirrhosis(HR = 2.00, 1.94 and 1.97, respectively; all P < 0.001).Additionally, we found that heavy smoking resulted in a significantly worse overall survival in those patients carrying variantalleles of rs7085725(HR = 2.07, 95%CI: 1.13-3.76, P = 0.018).The distributions of GSTO2 : rs7085725 and GSTP1 : rs4147581 genotypes were associated with altered gene expression and contributed to influences on overall ***: Our study provides the first evidence that GSTO2 and GSTP1 gene polymorphisms may serve as independent prognostic markers for HCC patients.

关键词： Glutathione S-transferase Polymorphism Hepatocellu

Key Conditions in Capillary Electrophoresis of Amino Acids with Indirect Ultraviolet Detection

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Chinese Chemical Letters 2000年第9期11卷 803-806页

作者： zhi xin wang Yi CHEN Qing GUO Center for Molecular Science. Institute of Chemistry. The Chincse Academy of Sciences. Beijing 100080 Chinese Acad Sci Inst Chem Ctr Mol Sci Beijing 100080 Peoples R China

Key factors for capillary electrophoresis of amino acids with indirect ultraviolet detection were illustrated. Buffer compositions and additives were found to be influential as expected, while buffer pH was shown to be particularly critical to the separation and detection. The effect of pH also depended on the electrophoretic mode used. Ways to obtain recurring and selective were suggested.

关键词： amino acids capillary electrophoresis indirect ultraviolet detection

FoxM1 overexpression promotes epithelial-mesenchymal transition and metastasis of hepatocellular carcinoma

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World Journal of Gastroenterology 2015年第1期21卷 196-213页

作者： Fan-Di Meng Ji-Chao Wei Kai Qu zhi-xin wang Qi-Fei Wu Ming-Hui Tai Hao-Chen Liu Rui-Yao Zhang Chang Liu Department of Hepatobiliary Surgery the First Affiliated HospitalSchool of MedicineXi’an Jiaotong University Department of Thoracic Surgery the First Affiliated Hospital of Xi’an Jiaotong University

AIM: To investigate the expression of forkhead box protein M1(Fox M1) in the process of epithelial mesenchymal transition in hepatocellular carcinoma(HCC) and its role in ***: Fox M1 and E-cadherin expression in HCC tissue microarray specimens was evaluated by immunohistochemical staining,and statistical methods were applied to analyze the correlation between FoxM 1 and epithelial-mesenchymal transition(EMT).KaplanMeier analysis of the correlation between the Fox M1 expression level and recurrence or overall survival of HCC patients was *** expression of FoxM 1,E-cadherin and snail homologue 1(SNAI1) in HCC cell lines was evaluated by real-time reverse transcriptionpolymerase chain reaction and Western *** growth factor(HGF) was used to induce EMT and stimulate cell migration in HCC *** expression of Fox M1 and SNAI1 was regulated by transfection with plasmids pc DNA3.1 and si RNAs in *** occurrence of EMT was evaluated by Transwell assay,morphologic analysis and detection of the expression of EMT markers(E-cadherin and vimentin).Luciferase and chromatin immunoprecipitation assays were used to evaluate whether SNAI1 is a direct transcriptional target of FoxM ***: FoxM 1 expression was increased significantly in HCC compared with para-carcinoma(10.7 ± 0.9 vs 8.2 ± 0.7,P < 0.05) and normal hepatic(10.7 ± 0.9 vs 2.7 ± 0.4,P < 0.05) *** of Fox M1 was correlated with HCC tumor size,tumor number,macrovascular invasion and higher TNM stage,but was negatively correlated with E-cadherin expression in microarray specimens and in cell *** M1 overexpression was correlated significantly with HCC metastasis and *** vitro,we found that FoxM 1 plays a key role in HGF-induced EMT,and overexpression of Fox M1 could suppress E-cadherin expression and induce EMT changes,which were associated with increased HCC cell ***,we confirmed that FOXM1 directly binds to and activates the SNAI1 promot

关键词： Forkhead box protein M1 Hepatocellular carcinoma E

The Intrinsically Disordered Protein BKI1 Is Essential for Inhibiting BRI1 Signaling in Plants

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Molecular Plant 2015年第11期8卷 1675-1678页

作者： Jianjun Jiang Tao wang zhihua Wu Jie wang Chi Zhang Haijiao wang zhi-xin wang Xuelu wang State Key Laboratory of Genetic Engineering and Department of Genetics School of Life Sciences Fudan University Shanghai 200438 China College of Life Science and Technology Huazhong Agricultural University Wuhan 430070 China MOE Key Laboratory for Protein Science School of Life Sciences Tsinghua University Beijing 100084 China

Dear Editor,Brassinosteroids （BRs） are a class of essential polyhydroxylated steroid hormones regulating plant growth and development, and their responses to environmental cues （Clouse, 2011 ; Yang et al., 2011）. BRs are perceived at the plasma membrane by a receptor complex, which consists of two leucine-rich receptor-like kinases （LRR-RLKs）, BRASSINOSTEROID INSENSITIVE 1 （BRI1） and BRI1-ASSOCIATED RECEPTOR KINASE 1 （BAK1）.

关键词：激素类亮氨酸磷酸转移酶类内皮缩血管肽类生长和发育细胞膜胰岛素植物