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DIC imaging for identification of motor and sensory nerves

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Journal of Innovative Optical Health Sciences 2016年第5期9卷 1-7页

作者： Dayu Chen Yuxiang Wu Tao Sui Shaoqun Zeng Xiaojian Cao xiaohua lv Department of Neurosurgery Wuhan General Hospital of Guangzhou Military Command of PLA 627 Wuluo Road Wuhan 430070P.R.China Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and Technology Wuhan 430074 P.R.China School of Physical Education Jianghan University Wuhan 430056P.R.China First A±liated Hospital of Nanjing Medical University Nanjing 210029 P.R.China

Identification of motor and sensory nerves is important in applications such as nerve injury *** practice relies on time consuming staining methods for this ***,we use laser scanning infrared diferential interference contrast(IR-DIC)microscopy for label-free observation of the two types of *** and dorsal nerve roots of adult beagle dogs were collected and sections of different thicknesses were imaged with an IR-DIC *** texture patterns of the IR-DIC images of the motor and sensory nerve can be distinguished when the section thickness increases to 40 *** suggests that nerve fibers in motor and sensory nerves have different distribution *** result hints a potential new way for more rapid identification of nerve type in peripheral nerve repair surgery.

关键词： Differential interference microscopy nerve repair nerve root image pattern.

Direction Selectivityof TmY Neurites in Drosophila

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Neuroscience Bulletin 2023年第5期39卷 759-773页

作者： Yinyin Zhao Shanshan Ke Guo Cheng xiaohua lv Jin Chang Wei Zhou Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and TechnologyWuhan 430074China

The perception of motion is an important func-tion of *** wiring diagrams for extracting direc-tional information have been obtained by connectome *** selectivity in Drosophila is thought to originate in T4/T5 neurons through integrating inputs with different temporal filtering *** genetic screening based on synaptic distribution,we isolated a new type of TmY neuron,termed TmY-ds,that form recipro-cal synaptic connections with T4/T5 *** neurites responded to grating motion along the four cardinal direc-tions and showed a variety of direction ***-ingly,its direction selectivity originated from temporal filtering neurons rather than T4/*** silencing and activation experiments showed that TmY-ds neurons are functionally upstream of T4/*** results suggest that direction selectivity is generated in a tripartite circuit formed among these three neurons—temporal filtering,TmY-ds,and T4/T5 neurons,in which TmY-ds plays a role in the enhancement of direction selectivity in T4/T5 neurons.

关键词： Direction selectivity TmY-ds-Synaptic connection Two-photon calcium imaging Drosophila

Neural spike train reconstruction from calcium imaging via a signal-shape composition model

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Science China(Life Sciences) 2020年第12期63卷 1829-1832页

作者： Yu Shen Tingwei Quan Meng Wang Xiuli Liu xiaohua lv Xiaowei Chen Shaoqun Zeng Britton Chance Center far Biomedical Photonics Wuhan National Laboratory for Optoelectronics Huazhong University of Science and TechnologyWuhan 430074China MoE Key Laboratory for Biomedical Photonics Department of Biomedical Engineering.Huazhong University of Science and TechnologyWuhan 430074China Brain Research Center and State Key Laboratory of Trauma Burnsand Combined InjuryThird Military Medical UniversityChongqing 400038China College of Mathematics and Economics Hubei University of EducationWuhan 430205China

Dear Editor,Since calcium imaging can simultaneously record the calcium signals of several hundred neurons, it has been a powerful tool in measuring the functional activity of neural populations. However, calcium imaging is an indirect measurement of neuronal firing activity, and this technique requires reconstructing the AP trains from the corresponding calcium fluorescence signals of the recorded neurons.

关键词： record shape neural

Research on vibration energy harvesting technology of power equipment based on alternating magnet array

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High Voltage 2024年第2期9卷 336-345页

作者： Pinlei lv Chengyu Fan Aijun Yang Huan Yuan Jifeng Chu Mingzhe Rong xiaohua Wang State Key Laboratory of Electrical Insulation and Power Equipment Xi'an Jiaotong UniversityXi'anChina State Grid Sichuan Electric Power Research Institute ChengduChina

Vibration with a frequency of 100 Hz is widely distributed in the power equipment,and it can provide a new way to supply energy for sensors by vibration energy *** vibration energy harvesting method based on electromagnetic induction principle was studied through the arrayed structure of magnets and *** magnetic field models were established for four magnet array structures and it was found that the alternating magnet array has the largest magnetic flux and magnetic flux *** on the alternating magnet array,prototypes of energy harvester with vertical and parallel movement mode were *** structural parameter optimisation analysis,two different energy harvesters were fabricated and it was found that the energy harvester with a parallel movement mode has better output *** energy harvester could provide output voltage/current and power of 8.35 V/17.39 mA and 15.13 mW(matched resistance is 200Ω)at an acceleration of 5 m⋅s^(-2).The 100 mF capacitor could be charged to 2.72 V within 300 s,and the final voltage of the capacitor is greater than 3 V,which could sustainably drive commercial wireless temperature/humidity sensors.

关键词： power energy alternating

Fault Analysis and Maintenance of DZZ Series of Automatic Weather Stations

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Meteorological and Environmental Research 2018年第3期9卷 35-37页

作者： lv Weiwei lv xiaohua Tang Zuoyang Xie xiaohua Xiao Yong Xinshao Meteorological Office Xinshao 422900China Shaoyang Meteorological Office Shaoyang 422000China

The instruments of regional automatic weather station are placed outside to measure daily changes of meteorological factors. Due to common influence of sun,wind,rain and other factors,it is very easy to cause various faults and damages of the instrument. The construction of regional automatic weather station network plays an important role in improving forecast accuracy,servicing local government and ecological civilization construction,providing scientific disaster prevention and relief decision-making basis for government department. In this paper,based on daily operation situation of regional automatic weather station in Shaoyang region,combining communication,hardware and software,fault situation of collector,temperature and humidity sensor,wind sensor and rainfall sensor in automatic weather station is analyzed in detail. Moreover,some common fault cases are listed and analyzed,some troubleshooting methods are summarized,and daily maintenance measures are proposed.

关键词： DZZ series Automatic weather station Instrument fault Analysis Maintenance

Divergent Projection Patterns Revealed by Reconstruction of Individual Neurons in Orbitofrontal Cortex

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Neuroscience Bulletin 2021年第4期37卷 461-477页

作者： Junjun Wang Pei Sun xiaohua lv Sen Jin Anan Li Jianxia Kuang Ning Li Yadong Gang Rui Guo Shaoqun Zeng Fuqiang Xu Yu-Hui Zhang Britton Chance Center for Biomedical Photonics Wuhan National Laboratory for Optoelectronics-Huazhong University of Science and TechnologyWuhan430074China MoE Key Laboratory for Biomedical Photonics School of Engineering SciencesHuazhong University of Science and TechnologyWuhan430074China Centre for Brain Science State Key Laboratory of Magnetic Resonance and Atomic Molecular PhysicsKey Laboratory of Magnetic Resonance in Biological SystemsWuhan Institute of Physics and MathematicsCAS Centre for Excellence in Brain Science and Intelligence TechnologyChinese Academy of SciencesWuhan430071China

The orbitofrontal cortex(OFC)is involved in diverse brain functions via its extensive projections to multiple target *** is a growing understanding of the overall outputs of the OFC at the population level,but reports of the projection patterns of individual OFC neurons across different cortical layers remain ***,by combining neuronal sparse and bright labeling with a whole-brain florescence imaging system(fMOST),we obtained an uninterrupted three-dimensional whole-brain dataset and achieved the full morphological reconstruction of 25 OFC pyramidal *** compared the wholebrain projection targets of these individual OFC neurons in different cortical layers as well as in the same cortical *** found cortical layer-dependent projections characterized by divergent patterns for information *** study not only provides a structural basis for understanding the principles of laminar organizations in the OFC,but also provides clues for future functional and behavioral studies on OFC pyramidal neurons.

关键词： Orbitofrontal cortex Whole-brain imaging Morphological reconstruction Output Projection pattern

Cause Analysis of a Double-cyclone Type of Rainstorm Process with Easterly Wave

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Meteorological and Environmental Research 2019年第2期10卷 15-19,26页

作者： Jin Xuanxi lv Weiwei lv xiaohua Tang Zuoyang Chen Qing Shaoyang Meteorological Bureau Xinning Meteorological Bureau

On the basis of comprehensive analysis on weather background and main influencing system of rainstorm formation,using NCEP reanalysis data,rainfall data of Shaoyang ground regional automatic station,a new generation of Doppler weather radar product information,etc.,high-altitude weather situation and physical formation mechanism of local double-cyclone type of rainstorm process with easterly wave in Shaoyang area on August 14,2016 were analyzed in detail,to find out prophase signal features which had indicating significance for rainstorm forecast. The results showed that:(1) the position of zonal subtropical high pressure in east-west direction was the necessary condition for the formation of easterly wave.(2) The rainstorm formation was closely related to double tropical depressions acting in the south of subtropical high pressure,and westward movement of easterly wave over 500 hPa and low-pressure inverted trough at low layer were main impact systems of the rainstorm.(3) Continuous flow of water vapor from the South China Sea to northwest was the source of water vapor required by this rainstorm.(4) Numerical prediction model products had certain guiding significance for forecasting the heavy precipitation,with important reference value.(5) Doppler radar products had strong forecasting ability in the nowcasting of rainstorm falling zone,which was an important measure of correcting forecast errors.

关键词： Rainstorm Diagnostic analysis Subtropical high pressure Easterly wave Water vapor source

Analysis of Climatic Characteristics of Fog and Haze Weather in Shaoyang in the Past 60 Years and Its Forecast

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Meteorological and Environmental Research 2018年第3期9卷 1-4,9页

作者： lv xiaohua Tang Zuoyang lv Weiwei Tan Dequan Tang Yaqiong Shaoyang Meteorological Office Shaoyang 422000China

Based on conventional observation data and NCEP reanalysis data at 10 national basic stations and reference stations of Shaoyang City during 1951-2014,300 cases of typical regional dense fog process appeared in the history were selected. From meteorological factors and weather situation,temporal-spatial distribution characteristics and trend change characteristics of dense fog in Shaoyang were analyzed. The results showed that（ 1） temporal-spatial distribution of dense fog in Shaoyang region was uneven,and interannual variability of fog days had large volatility and bad periodicity; dense fog days in Shaoyang region was obviously more in winter half year and less in summer half year. Dense fog was the most in November and the least in July. Dense fog mostly concentrated during 03： 00-09： 00; appearance time mostly concentrated during 05： 00-07： 30,and dissipation time mostly appeared after 08： 30. Dense fog appeared early and dissipated late in winter half year,and vice verse in summer half year.（ 2） Seen from meteorological factors,ground and 850 h Pa of wind velocity was generally 0-3 m/s,which was all small. Moreover,there existed temperature inversion from ground to 850 h Pa. Relative humidity on dense fog day was larger,and precipitation or cloudy day mostly appeared in prior day.（ 3） There were four kinds of ground weather situation forming dense fog： uniform pressure field type,cold and high pressure bottom type,cold and high pressure rear type,frontal type. Based on grasping change characteristics,rule and formation reason of dense fog,some forecast focus was found.

关键词： Dense fog Climatic characteristics Temporal-spatial distribution Weather situation Forecast

Characteristics of Rainstorm and Flood Disaster in Shaoyang City and Flood Protection Countermeasures

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Meteorological and Environmental Research 2018年第3期9卷 75-78,82页

作者： Tang Zuoyang lv Weiwei lv xiaohua Tang Yaqiong Tan Dequan Shaoyang Meteorological Office Shaoyang 422000China Xinshao Meteorological Office Xinshao422900China

Based on ground meteorological observation data at 10 representative stations of Shaoyang City during 1951-2017 and rainfall data at over 220 automatic weather stations during 2005-2017,statistical analysis on rainstorm and flood disaster in Shaoyang was conducted. Occurrence characteristics and rule of rainstorm and flood disaster were found,and disaster situation was evaluated and analyzed. The results showed that（ 1）there was almost local rainstorm and flood in Shaoyang City every year,with strong seasonal characteristic.（ 2） The loss caused by rainstorm and flood disaster was the most serious in various meteorological disasters.（ 3） Rainstorm and flood disaster generally had the sudden and devastating characteristics. Via the analysis and research on rainstorm and flood disaster,defense measure and countermeasure of rainstorm and flood were proposed,which could provide scientific decision basis for party and government leading flood relief,and had important guidance significance for preventing and mitigating disasters by government agencies.

关键词： Rainstorm Flood disaster Urban flood control Flood control measures and countermeasures