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Interleukin-10 and chronic liver disease

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World Journal of Gastroenterology 2006年第11期12卷 1681-1685页

作者： Li-Juan Zhang xiao-zhong wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

Interleukin （IL）-10 is an important immunoregulatory cytokine produced by many cell populations. Numerous investigations suggest that IL-10 plays a major role in chronic liver diseases. IL-10 gene polymorphisms are possibly assodated with liver disease susceptibility or se-verity. Recombinant human IL-10 has been produced and is currently tested in clinical trials. These trials may give new insights into the immunobiology of IL-10 and suggest that the IL-10/IL-10 receptor system may become a new therapeutic target.

关键词： Interleukin (IL)-10 Cytokine Chronic liver diseases Polymorphisms

Possible mechanism for hepatitis B virus X gene to induce apoptosis of hepatocytes

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World Journal of Gastroenterology 2005年第28期11卷 4351-4356页

作者： Sheng-Jun Zhang Hong-Ying Chen Zhi-Xin Chen xiao-zhong wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To investigate the possible mechanism for HBV X gene to induce apoptosis of hepatocyte HL-7702 cells.METHODS： HBV X gene eukaryon expression vector pcDNA3-X was established and transfected into HL-7702 cells by lipid-mediated transfection, including transient and stable transfection. Positive clones were screened by incubating in the selective medium with 600 μg/mL G418 and named HL-7702/HBV-encoded X protein （HBx） cells. The expressions of Fas/FasL, Bax/Bcl-2, and c-myc mRNA were measured by semi-quantitative RT-PCR in HL-7702/HBx and control group, respectively.RESULTS： RT-PCR analysis confirmed that HBV X gene was transfected into HL-7702 cells successfully. By semiquantitative RT-PCR analysis, Bax and c-myc mRNA levels in HL-7702/HBx cells of transient transfection were significantly higher than those in control, FasL and c-myc mRNA levels in HL-7702/HBx cells of stable transfection were significantly higher than those in control, whereas the Bcl-2 mRNA levels in HL-7702/HBx cells of transient and stable transfection were significantly lower than thosein control.CONCLUSION： HBV X gene may promote the apoptosis of hepatocytes by regulating the expressions of Fas/FasL, Bax/Bcl-2, and c-myc gene in a dose-dependent manner.

关键词： Hepatitis B virus X gene Apoptosis Geneexpression

Accelerated effect on Mitsunobu reaction via bis-N-tert-butoxycarbonylation protection of 2-amino-6-chloropurine and its application in a novel synthesis of penciclovir

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Journal of Zhejiang University-Science A(Applied Physics & Engineering) 2013年第10期14卷 760-766页

作者： Li-yan DAI Qiu-long SHI Jing ZHANG xiao-zhong wang Ying-qi CHEN Department of Chemical and Biological Engineering Zhejiang University

Solubility of 2-amino-6-chloropurine in Mitsunobu solvents could be significantly improved after its exocyclic amino group is protected via *** bis-Boc protected 2-amino-6-chloropurine also shows excellent activity and N9 selectivity in the coupling with various alcohols by a Mitsunobu ***,a new practical and efficient method is established for the synthesis of penciclovir(PCV) from bis-Boc-2-amino-6-chloropurine 9 and the side chain of 5-(2-hydroxyethyl)-2,2-dimethyl-1,3-dioxane 5—the latter being a more easily prepared cyclic precursor of the diacetate side chain used in the conventional *** coupling of 9 with 5 proceeded regioselectively at a N9 position of purine derivative for a good yield under Mitsunobu conditions.

关键词： 2-amino-6-chloropurine Mitsunobu reaction bis-Boc protection Penciclovir(PCV)

Dicyclopentyl azodicarboxylate(DCpAD):A new alternative azo-reagent for the Mitsunobu reaction

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Chinese Chemical Letters 2011年第9期22卷 1047-1050页

作者： Jian Hai Yang Li Yan Dai xiao zhong wang Ying Qi Chen Department of Chemical and Biological Engineering Zhejiang University Hangzhou 310027 China

Dicyclopentyl azodicarboxylate is introduced as a new azo-reagent which can be conveniently prepared in two steps and be used in the Mitsunobu reaction. Though there are no distinct difference of reactivity between DC... 详细信息

关键词： Dicyclopentyl azodicarboxylatc Mitsunobu reaction Azo-reagent

Effect of IL-10 on the expression of HSC growth factors in hepatic fibrosis rat

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World Journal of Gastroenterology 2005年第31期11卷 4788-4793页

作者： Mei-Na Shi Wei-Da Zheng Li-Juan Zhang Zhi-Xin Chen xiao-zhong wang Department of Gastroenterology Union Hospital of Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To study the effect of IL-10 on the expression of growth factors - transforming growth factor-β1 （TGF-β1）, epidermal growth factor （EGF）, hepatocyte growth factor （HGF）and platelet-derived growth factor （PDGF） of hepatic stellate cells （HSCs） of hepatic fibrosis rat and the anti-fibrogenic role of exogenous IL-10. METHODS： Hepatic fibrosis was induced by CCI4 administration intra-peritoneally. Sixty clean male Sprague-Dawley （SD） rats were randomly divided into three groups： normal control group （GN, 8 rats）, hepatic fibrosis model group （GC, 28 rats） and IL-10 treated group （GI, 24 rats）. At the beginning of the 7^th and 11^th wk, rats in each group were routinely perfused with pronase E and type IV collagenase through a portal vein catheter and the suspension obtained from the liver was spun by centrifugation with 11% Nycodenz density gradient to isolate HSCs. Histological examination was used to determine the degree of hepatic fibrosis. RT-PCR was employed to analyze mRNA expression from freshly isolated cells. Immunocytochemistry was performed to detect protein expression in primary cultured HSCs. RESULTS： Rat hepatic fibrosis was developed with the increase of injection frequency of CCl4, and HSCs were successfully isolated. At the 7^th and 11^th wk, TGF-β1, EGF, and HGF mRNA in GC increased obviously compared with GN （P = 0.001/0.042, 0.001/0.001, 0.001/0.001） and GI （P= 0.001/0.007, 0.002/0.001, 0.001/0.001）. For TGF-β1, no difference was observed between GI and GN. For EGF, mRNA level in GI increased compared with GN during the 7^th wk （P= 0.005） and 11^th wk （P= 0.049）. For HGF, mRNA level in GI decreased compared with GN at the 7^th wk （P = 0.001） and 11^th wk （P= 0.021）. Between these two time points, TGF-β1 expression at the 7^th wk was higher than that of the 11^th wk （P = 0.049）, but for EGF, the former was lower than the latter （P = 0.022）. As for PDGF mRNA, there was no significant difference between thesegroups, but difference seemed to exist i

关键词： Hepatic fibrosis Hepatic stellate cells Interleukin-10 Transforming growth factor-131 Epidermal growth factor Hepatocyte growth factor Platelet-derived growth factor

Apoptosis and its pathway in X gene-transfected HepG_2 cells

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World Journal of Gastroenterology 2005年第28期11卷 4326-4331页

作者： Na Lin Hong-Ying Chen Dan Li Sheng-Jun Zhang Zhi-Xin Cheng xiao-zhong wang Department of Gastroenterology Affiliated Union Hospital Fujian Medical University Fuzhou 350001 Fujian Province China

AIM： To investigate the effect of hepatitis B virus （HBV） X gene on apoptosis and expressions of apoptosis factors in X gene-transfected HepG2 cells.METHODS： The HBV X gene eukaryon expression vector pcDNA3-X was transiently transfected into HepG2 cells by lipid-media transfection. Untransfected HepG2 and HepG2 transfected with pcDNA3 were used as controls. Expression of HBx in HepG2 was identified by RT-PCR. MTT and TUNEL were employed to measure proliferation and apoptosis of cells *** groups. Semi-quantified RT-PCR was used to evaluate the expression levels of Fas/FasL, Bax/Bcl-xL, and c-myc in each group.RESULTS： HBV X gene was transfected into HepG2 cells successfully. RT-PCR showed that HBx was only expressed in HepG2/pcDNA3-X cells, but not expressed in HepG2 and HepG2/pcDNA3 cells. Analyzed by MTT, cell proliferationcapacity was obviously lower in HepG2/pcDNA3-X cells （0.08910±0.003164） than in HepG2 （0.14410±0.004927） and HepG2/pcDNA3 cells （0.12150±0.007159）（P〈0.05 and P〈0.01）. Analyzed by TUNEL, cell apoptosis was much more in HepG2/pcDNA3-X cells （980/2 000） than HepG2 （420/2 000）, HepG2/pcDNA3 cells （520/2 000）（P〈0.05 and P〈0.01）. Evaluated by semi-quantified RT-PCR, the expression level of Fas/FasL was significantly higher in HepG2 cells transfected with HBx than in HepG2 and HepG2/pcDNA3 cells （P〈0.05 and P〈0.01）. Bax/Bcl-xL expression level was also elevated in HepG2/pcDNA3-X cells （P〈0.05 and P〈0.01）. Expression of c-myc was markedly higher in HepG2/pcDNA3-X cells than in HepG2 and HepG2/pcDNA3 cells （P〈0.05 and P〈0.01）.CONCLUSION： HBV X gene can impair cell proliferation capacity, improve cell apoptosis, and upregulate expression of apoptosis factors. The intervention of HBV X gene on the expression of apoptosis factors may be a possible mechanism responsible for the change in cell apoptosis and proliferation.

关键词： HBx Transfect HepG2 Apoptosis Fas FasL Bax Bcl-xL c-mvc

Development of a novel score for the diagnosis of bacterial infection in patients with acute-on-chronic liver failure

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World Journal of Gastroenterology 2020年第32期26卷 4857-4865页

作者： Su Lin Yan-Yan Yan Yin-Lian Wu Ming-Fang wang Yue-Yong Zhu xiao-zhong wang Department of Gastroenterology Union Hospital of Fujian Medical UniversityFuzhou 350000Fujian ProvinceChina Liver Research Center The First Affiliated Hospital of Fujian Medical UniversityFuzhou 350000Fujian ProvinceChina Clinical Liver Center The 180th Hospital of People’s Liberation ArmyQuanzhou Fujian Province362100China

BACKGROUND The diagnosis of bacterial infection is difficult in patients with acute-on-chronic liver failure(ACLF).AIM To evaluate the diagnostic accuracy of widely used parameters for bacterial infection in ACLF and to develop a simple scoring system to improve diagnostic *** This was a retrospective ***(PCT),white blood cells(WBC),proportion of neutrophils(N%),and C-reactive protein(CRP)were *** regression was used to select variables for the scoring models and receiver operating characteristic curve(ROC)analysis was used to evaluate the diagnostic value of different *** This study included 386 patients with ACLF,169(43.78%)of whom had bacterial infection on *** area under the ROC(AUROC)of PCT,CRP,WBC and N%for the diagnosis of bacterial infection ranged from 0.637 to 0.692,with no significant difference between *** regression showed that only N%,PCT,and CRP could independently predict infection.A novel scoring system(infection score)comprised of N%,PCT and CRP was *** AUROC of the infection score was 0.740,which was significantly higher than that for the other four indices(infection score vs N%,PCT,CRP,and WBC,P=0.0056,0.0001,0.0483 and 0.0008,respectively).The best cutoff point for the infection score was 4 points,with a sensitivity of 78.05%,a specificity of 55.29%,a positive predictive value of 57.91%and a negative predictive value of 76.16%.CONCLUSION The infection score is a simple and useful tool for discriminating bacterial infection in ACLF.

关键词： Acute on chronic liver failure Bacterial infection Score

Relationship between transforming growth factorβ1 and antifibrotic effect of interleukin-10

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World Journal of Gastroenterology 2006年第15期12卷 2357-2362页

作者： Mei-Na Shi Yue-Hong Huang Wei-Da Zheng Li-Juan Zhang Zhi-Xin Chen xiao-zhong wang Department of Gastroenterology Union Hospital of Fujian Medical UniversityFuzhou 350001 Fujian Province China

AIM： To study the effect of interleukin-10 （IL-10） on the expression of transforming growth factor β1 （TGF-β1） in hepatic fibrosis rats and the anti-fibrotic role of exogenous IL-10. METHODS： Hepatic fibrosis was induced by carbon tetrachloride administered （CCh） intraperitoneally. The experiment was performed in two stages. In the first stage, 60 SD rats were divided randomly into normal control group I（GNI, n = 8）, hepatic fibrosis group（GC, n = 28）and IL-10 intervened group（GI, n = 24）. At the beginning of the 7^th and 11^th wk, hepatic stellate cells （HSCs） were isolated, reverse transcription-polymerase chain reation （RT-PCR） and immunocytochemistry were performed to detect the expression of TGF-β1 in HSCs. Histological examination was used to determine the degree of hepatic fibrosis. In the second stage, 47 SD rats were divided randomly into normal control group 2 （GN2, n = 6）and CCh group（GZ, n = 41）. At the end of the 9th week, rats in GZ group were allocated randomly into model group（GM, n = 9）, IL-10 treatment group（GT, n = 9） and recovered group （GR, n = 9）. At the end of the 12^th week, all rats were sacrificed. RT-PCR and immuno- histochemistry were performed to detect the expression of TGF-β1 in liver tissue. ELISA was used to assay serum TGF-β1 levels. RESULTS： Hepatic fibrosis developed in rats with the increase of the injection frequency of CCI4. In the first stage, hepatic fibrosis developed and HSCs were isolated successfully. At the 7^th and 11^th week, TGF-β1 mRNA in GC group increased significantly compared with that in GN1（P = 0.001/0.042） and GI groups（P = 0.001/0.007）, whereas there was no significant difference between the two groups. The levels of TGF-β1 at the beginning of the 7^th wk was higher than that of the 11^th wk （P = 0.049）.Immunocytochemistry results of TGF-β1 were consistent with the above findings. In the second stage, TGF-β1 increased significantly in GM group compared to GN2. Alter treatment with IL-10, TGF-β1 declined obvio

关键词： Hepatic fibrosis Hepatic stellate cells Interleukin-10 Transforming growth factor-β1

Clinical characteristics and current management of hepatitis B and C in China

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World Journal of Gastroenterology 2014年第37期20卷 13582-13590页

作者： Yong-Tao Sun Yue-Xin Zhang Hong Tang Qing Mao xiao-zhong wang Ling-Yi Zhang Hong Chen Ying-Na zhong Shu-Mei Lin Da-Zhi Zhang Department of Infectious Diseases Tangdu HospitalAffiliated to the Fourth Military Medical University the First Affiliated Hospital of Xinjiang Medical University West China Hospital Sichuan University the First Affiliated Hospital of the Third Military Medical University Traditional Chinese Medicine Hospital of Xinjiang Uygur Administrative Region the Second Affiliated Hospital of Lanzhou University the First Affiliated Hospital of Lanzhou University People’s Hospital of Xinjiang Uygur Administrative Region the First Affiliated Hospital of Xi'an Jiaotong University the Second Affiliated Hospital of Chongqing Medica University

AIM: To describe a population of outpatients in China infected by hepatitis B virus (HBV) and/or hepatitis C virus (HCV), and assess their current management status. METHODS: A multicenter, cross-sectional study of HBV- and/or HCV-infected patients was conducted from August to November, 2011 in western China. Patients >= 18 years of age with HBV and/or HCV infections who visited outpatient departments at 10 hospitals were evaluated, whether treated or not. Data were collected on the day of visit from medical records and patient interviews. RESULTS: A total 4010 outpatients were analyzed, including 2562 HBV- infected and 1406 HCV-infected and 42 HBV/HCV co-infected patients. The median duration of documented infection was 7.5 years in HBV- infected and 1.8 years in HCV-infected patients. Cirrhosis was the most frequent hepatic complication (12.2%), appearing in one-third of patients within 3 years prior to or at diagnosis. The HCV genotype was determined in only 10% of HCV-infected patients. Biopsy data were only available for 54 patients (1.3%). Antiviral medications had been received by 58.2% of patients with HBV infection and 66.6% with HCV infection. Nucleos(t) ide analogs were the major antiviral medications prescribed for HBV- infected patients (most commonly adefovir dipivoxil and lamivudine). Ribavirin + pegylated interferon was prescribed for two-thirds of HCV-infected patients. In the previous 12 mo, around one-fifth patients had been hospitalized due to HBV or HCV infection. CONCLUSION: This observational, real-life study has identified some gaps between clinical practice and guideline recommendations in China. To achieve better health outcomes, several improvements, such as disease monitoring and optimizing antiviral regimens, should be made to improve disease management. (C) 2014 Baishideng Publishing Group Inc. All rights reserved.

关键词： Hepatitis B Hepatitis C Clinical characteristics Treatment