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Original article： Perichondrium/cartilage composite graft for repairing large tympanic membrane perforations and hearing improvement

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Chinese Medical Journal 2010年第3期 301-304页

作者： CHEN xiao-wei YANG Hua gao Ru-zhen YU Rong gao Zhi-qiang Department of Otolaryngology-Head and Neck Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100730 China

Background The main risk factors for postoperative failure in tympanoplasties are large perforations that are difficult to repair, annular perforations, and a tympanic membrane （TM） with extensive granular myringitis that require middle ear exploration and mastoidectomy. The aim of this study was to investigate a novel technique of perichondrium/cartilage composite graft for repairing the large TM perforation in the patient of otitis media. Methods Retrospective chart reviews were conducted for 102 patients with large tympanic membrane perforations, who had undergone tympanoplasty from August 2005 to August 2008. Tympanoplasty or tympanomastoidectomy using a perichondrium/cartilage composite graft was analyzed. The tragal or conchal perichondrium/cartilage was used to replace the tympanic membrane in patients. Results Patients aged from 13 to 67 years were followed up in average for 24 months （10-36 months）. Seventy-four ears （72.61%） were used the tragal perichondrium/cartilage as graft material and 27 ears （27.39%） were used the conchal perichondrium/cartilage. Graft take was successful in all patients. Postoperative complications such as wound infection, hematoma, or sensorineural hearing loss were not identified. Nine patients （8.82%） had the partial ossicular replacement prosthesis, 14 patients （13.72%） using the autologous curved incus and 79 patients （77.45%） without prosthesis. Successful closure occurred in 92% of the ears. A total of 85.8% patients achieved a postoperative hearing improvement. Conclusions The graft underlay tympanoplasty using perichondrium/cartilage composite is effective for the majority of patients with large perforation. The hearing was improved even if the mastoidectomy was required in the patients with otitis media with extensive granulation.

关键词： otitis media perichondrium/cartilage tympanoplasty tympanomastoidectomy hearing improvement

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Comparative analysis of different feeder layers with 3T3 fibroblasts for culturing rabbits limbal stem cells

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International Journal of Ophthalmology(English edition) 2017年第7期10卷 1021-1027页

作者： Hui-Xian Wang xiao-wei gao Bing Ren Yan Cai Wen-Jing Li Yu-Li Yang Yi-Jian Li Medical College of Shihezi University Ophthalmic Center No.474 Hospital of Chinese PLA Southwest Hospital Third Military Medical University

AIM： To explore the possibility of human umbilical cord mesenchymal stem cells（h UCMSCs）, human umbilical vein endothelial cells（h UVECs）, human dental pulp stem cells（h DPSCs） and human periodontal ligament stem cells（h PDLSCs） serving as feeder cells in co-culture systems for the cultivation of limbal stem ***： Different feeder layers were cultured in Dulbecco＇s modified Eagle＇s medium（DMEM）/F12 and were treated with mitomycin C. Rabbits limbal stem cells（LSCs） were co-cultured on h UCMSCs, h UVECs, h DPSCs, h PDLSCs and NIH-3T3, and then comparative analysis were made between each group to see their respective colony-forming efficiency（CFE） assay and immunofluorescence（IPO13,CK3/12）.RESULTS： The efficiency of the four type cells in supporting the LSCs morphology and its cellular differentiation was similar to that of NIH-3T3 fibroblasts as demonstrated by the immunostaining properties analysis, with each group exhibiting a similar strong expression pattern of IPO13, but lacking CK3 and CK12 expression in terms of immunostaining. But h UCMSCs, h DPSCs and h PDLSCs feeder layers were superior in promoting colony formation potential of cells when compared to h UVECs and feedercell-free ***： hUCMSCs, hDPSCs and hPDLSCs can be a suitable alternative to conventional mouse NIH-3T3 feeder cells, so that risk of zoonotic infection can be diminished.

关键词： limbal stem cells feeder layers umbilical cord mesenchymal stem cells umbilical vein endothelial cells dental pulp stem cells periodontal ligament stem cells

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Observation on ultrastructure and histopathology of cornea following femtosecond laser-assisted deep lamellar keratoplasty for acute corneal alkaline burns

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International Journal of Ophthalmology(English edition) 2016年第4期9卷 481-486页

作者： Wen-Jing Li Yu-Kun Hu Hui Song xiao-wei gao Xu-Dong Zhao Jing Dong Yun-Lin Guo Yan Cai Department of Ophthalmology the 474~(th) Hospital of the Chinese People's Liberation Army Department of Ophthalmology the 309~(th) Hospital of the Chinese People's Liberation Army

AIM： To demonstrate the changes in ultrastructure and histopathology of the cornea in acute corneal alkaline burns after femtosecond laser-assisted deep lamellar keratoplasty.·METHODS： The New Zealand white rabbits treated with alkaline corneal burn were randomized into two groups,Group A（16 eyes） with femtosecond laser-assisted deep lamellar keratoplasty 24 h after burn and Group B（16 eyes）without keratoplasty as controls. All eyes were evaluated with transmission electron microscopy（TEM） at 1, 2, 3,and 4wk follow-up, then all corneas were tested by hematoxylin and eosin staining histology.· RESULTS： The corneal grafts in Group A were transparent, while those in Group B showed corneal stromal edema and loosely arranged collagen fibers. One week after treatment, TEM revealed the intercellular desmosomes in the epithelial layers and intact non-dissolving nuclei in Group A. At week 4, the center of the corneas in Group A was transparent with regularly arranged collagen fibers and fibroblasts in the stroma. In Group B, squamous cells were observed on the corneal surface and some epithelial cells were detached.· CONCLUSION： Femtosecond laser-assisted deep lamellar keratoplasty can suppress inflammatory responses, prevent toxic substance-induced injury to the corneal endothelium and inner tissues with quicker recovery and better visual outcomes.

关键词： femtosecondlaser deeplamellarkeratoplasty transmission electron microscopy alkali burn cornea rabbit

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Existence of Positive Solutions for Boundary Value Problems on the Semi-infinite Interval

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Chinese Quarterly Journal of Mathematics 2005年第4期20卷 380-384页

作者： NI xiao-hong GE wei-gao Department of Mathematics Huzhou Teacher＇s College Huzhou 313000 China

In this paper, the authors study the existence of positive solution of the following BVP {1/p（t）（P（t）x′）′＋f（t,x（t）,p（t）x′（t））=0,o〈t〈＋∞ αx（0）-βlimt→0p（t）x′（t）=0,γ limt→＋∞x（t）＋δlimt→＋∞p（t）x′（t）=0 on the semi-infinite interval. By considering characterization of the nonlinearity, they obtain some new existence results.

关键词： Sturm-Liouville boundary value problem positive solution fixed point

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BEM-EDM COUPLED ANALYSIS OF MULTI-SCALE PROBLEMS

BEM-EDM COUPLED ANALYSIS OF MULTI-SCALE PROBLEMS

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力学与工程——数值计算和数据分析2019学术会议

作者： xiao-wei gao YONG-TONG ZHENG State Key Laboratory of Structural Analysis for Industrial Equipment Dalian University of Technology

In this paper, the Element Differential Method(EDM) is coupled with the Multi-Domain Boundary Element Method(MDBEM) for solving general multi-scale heat conduction and mechanical problems. The basic algebraic equations in BEM are formulated in terms of displacements/temperatures and tractions/fluxes, which are the same as those in EDM. So when coupling these two methods, we don’t need to transform the variables like the equivalent nodal force into the surface traction as done in the finite element method(FEM). The key task in the proposed coupling method is to use the displacement consistency condition and the traction equilibrium equation at interface nodes of two methods to eliminate all BEM nodes except for those on the interfaces. The detailed elimination process is presented in this paper, which can result in the final system of equations without iteration. The coefficient matrix of the final coupled system is sparse even though a small part is dense. The coupling method inherits the advantage of EDM in flexibility and computational efficiency, and the advantage of BEM in the robustness of treating multi-scale problems. A number of numerical examples of general heat conduction and mechanical problems are given to demonstrate the correctness and efficiency of this coupling method.

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Recent progresses in stem cell research and hearing restoration

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Journal of Otology 2008年第1期3卷 1-8页

作者： YANG Hua, CHEN xiao-wei, gao Zhi-qiang Department of Otolaryngology, Chinese Academy of Medical Sciences, Peking Union Medical College Hospital, Beijing, China, 100730 Department of Otolaryngology Chinese Academy of Medical Sciences Peking Union Medical College Hospital Beijing 100730 China

Serious hearing and balance impairments can occur as a result of loss of hair cells related to aging, environmental stresses(such as noises exposure) or exposure to chemotherapeutic drugs(such as cisplatin and aminoglycoside antibiotics). Because a large portion of hearing impairment involves loss of hair cells, regeneration or replacement of these cells is a possible alternative to prosthetic devices1. Researchers have paid hot attention to the study of inner ear hair cell regeneration for hearing restoration for many years. Besides the important function of hair cells, there are many other contributors essential for normal hearing. Hair cell regeneration is one of the essential steps for total hearing recovery, but not the only one. In this article, we will review the progresses in research on hearing loss, stem cell application and hearing restoration.

关键词： stem cell Recent progresses in stem cell research and hearing restoration

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一种无人机路径规划算法研究

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系统仿真学报 2004年第1期16卷 20-21,34页

作者：符小卫高晓光西北工业大学电子与信息学院西安710072

指出了飞行器航迹规划与路径规划的区别;提出了一种给定威胁分布下的无人机路径规划算法。根据威胁分布情况构造无人机可能飞行的航路集,用voronoi图表示出来,采用Dijkstra算法搜索威胁分布图,求解粗略最短路径。在粗略最短路径的基础上... 详细信息

指出了飞行器航迹规划与路径规划的区别;提出了一种给定威胁分布下的无人机路径规划算法。根据威胁分布情况构造无人机可能飞行的航路集,用voronoi图表示出来,采用Dijkstra算法搜索威胁分布图,求解粗略最短路径。在粗略最短路径的基础上,应用三次样条曲线和序列二次规划的方法求解最优路径。用Matlab进行仿真验证,证明了算法的有效性。

关键词：无人机路径规划 voronoi图 Dijkstra算法三次样条曲线序列二次规划

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Electrochemical conversion of methane to bridge the gap in the artificial carbon cycle

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Journal of Energy Chemistry 2025年第1期100卷 286-308页

作者： Yuhao Peng Yuefeng Song Ihar Razanau Juanxiu xiao wei xiao Di Hu Guoxiong Wang College of Chemistry and Molecular Sciences Hubei Key Laboratory of Electrochemical Power Sources Wuhan University State Key Laboratory of Catalysis Dalian National Laboratory for Clean Energy iChEM (Collaborative Innovation Center of Chemistry for Energy Materials) Dalian Institute of Chemical Physics Chinese Academy of Sciences Laboratory of Physical-Chemical Technologies SSPA Scientific and Practical Materials Research Centre of NAS of Belarus State Key Laboratory of Marine Resources Utilization in South China Sea Collaborative Innovation Center of Marine Science and Technology School of Marine Science and Engineering Hainan University Department of Chemical and Environmental Engineering University of Nottingham Ningbo China

Methane, an abundant one-carbon（C1） resource, is extensively used in the industrial production of vital fuels and value-added chemicals. However, current industrial methane conversion technologies are energy-and carbon-intensive, mainly due to the high activation energy required to break the inert C–H bond, low selectivity, and problematic side reactions, including CO2emissions and coke deposition. Electrochemical conversion of methane（ECM） using intermittent renewable energy offers an attractive solution, due to its modular reactor design and operational flexibility across a broad spectrum of temperatures and pressures. This review emphasizes conversion pathways of methane in various reaction systems, highlighting the significance and advantages of ECM in facilitating a sustainable artificial carbon cycle. This work provides a comprehensive overview of conventional methane activation mechanisms and delineates the complete pathways of methane conversion in electrolysis contexts. Based on surface/interface chemistry, this work systematically analyzes proposed reaction pathways and corresponding strategies to enhance ECM efficiency towards various target products, including syngas, hydrocarbons, oxygenates, and advanced carbon materials. The discussion also encompasses opportunities and challenges for the ECM process, including insights into ECM pathways, rational electrocatalyst design, establishment of benchmarking protocols, electrolyte engineering, enhancement of CH4conversion rates, and minimization of CO2emission.

关键词： Methane Electrochemical conversion Reaction mechanism Catalyst design Electrode Electrocatalysis

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Endoplasmic reticulum stress and autophagy in cerebral ischemia/reperfusion injury:PERK as a potential target for intervention

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Neural Regeneration Research 2025年第5期20卷 1455-1466页

作者： Ju Zheng Yixin Li Ting Zhang Yanlin Fu Peiyan Long xiao gao Zhengwei Wang Zhizhong Guan xiaolan Qi wei Hong Yan xiao Key Laboratory of Endemic and Ethnic Diseases Ministry of Education&Key Laboratory of Medical Molecular Biology of Guizhou ProvinceGuizhou Medical UniversityGuiyangGuizhou ProvinceChina Guizhou Center for Disease Control and Prevention GuiyangGuizhou ProvinceChina Department of Histology and Embryology Guizhou Medical UniversityGuiyangGuizhou ProvinceChina Collaborative Innovation Center for Prevention and Control of Endemic and Ethnic Regional Diseases Co-constructed by the Province and Ministry GuiyangGuizhou ProvinceChina

Several studies have shown that activation of unfolded protein response and endoplasmic reticulum(ER)stress plays a crucial role in severe cerebral ischemia/reperfusion *** occurs within hours after cerebral ischemia,but the relationship between ER stress and autophagy remains *** this study,we established experimental models using oxygen-glucose deprivation/reoxygenation in PC12 cells and primary neurons to simulate cerebral ischemia/reperfusion *** found that prolongation of oxygen-glucose deprivation activated the ER stress pathway protein kinase-like endoplasmic reticulum kinase(PERK)/eukaryotic translation initiation factor 2 subunit alpha(e IF2α)-activating transcription factor 4(ATF4)-C/EBP homologous protein(CHOP),increased neuronal apoptosis,and induced ***,inhibition of ER stress using inhibitors or by si RNA knockdown of the PERK gene significantly attenuated excessive autophagy and neuronal apoptosis,indicating an interaction between autophagy and ER stress and suggesting PERK as an essential target for regulating *** autophagy with chloroquine exacerbated ER stress-induced apoptosis,indicating that normal levels of autophagy play a protective role in neuronal injury following cerebral ischemia/reperfusion *** from this study indicate that cerebral ischemia/reperfusion injury can trigger neuronal ER stress and promote autophagy,and suggest that PERK is a possible target for inhibiting excessive autophagy in cerebral ischemia/reperfusion injury.

关键词： apoptosis ATF4 autophagy C/EBP homologous protein cerebral ischemia/reperfusion injury eIF2α endoplasmic reticulum stress PERK

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角膜缘干细胞体外培养的研究进展

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国际眼科杂志 2018年第9期18卷 1612-1615页

作者：周瑛高晓唯新疆医科大学临床医学院中国人民解放军第四七四医院眼科

角膜缘干细胞是位于角膜缘Vogt栅栏区的一种成体单能干细胞,其可通过自我更新以补充角膜上皮的损伤修复,在维持正常角膜上皮完整性、角膜透明度以及维持正常视力中发挥着重要作用。各种原因造成的角膜缘干细胞缺失将导致患者角膜混浊、... 详细信息

角膜缘干细胞是位于角膜缘Vogt栅栏区的一种成体单能干细胞,其可通过自我更新以补充角膜上皮的损伤修复,在维持正常角膜上皮完整性、角膜透明度以及维持正常视力中发挥着重要作用。各种原因造成的角膜缘干细胞缺失将导致患者角膜混浊、眼表血管化,最终失明,目前治疗相关疾病的主要方法为体外培养角膜缘干细胞后再行移植治疗,其中如何高效地体外扩增角膜缘干细胞成为治疗成功与否的关键,本文就角膜缘干细胞定位、获取方法以及体外扩增方式等进行综述。

关键词：角膜缘干细胞三维细胞培养体外扩增

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