检索结果-南通市图书馆

Engineered Regeneration 2022年第4期3卷 440-452页

作者： Pei wang xiansong wang Department of Plastic and Reconstructive Surgery Shanghai Key Laboratory of Tissue EngineeringShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China Department of Thoracic surgery Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China

Bone is a complex biological tissue with a complicated hierarchical nanocomposite *** native microen-vironment of the bone tissue may be significantly disrupted by large physiological and pathological bone *** defects are often treated via complex surgical procedures that involve the application of autografts or *** these grafting procedures often suffer from insufficient natural bone stock and ***,these traditional treatment methods fail to simulate a regenerative microenvironment,which plays a significant role in regeneration of bone tissue and repair of large bone *** this end,various biomimetic scaffolds have been devised to mimic the native microenvironment of bone and thereby to simultaneously re-pair bone defects and promote bone *** propose here a novel concept,in vivo bone regenerative microenvironment(BRM),which enables repair of large bone defects and enhances new bone tissue formation with external *** this review,we mainly focus on materials and methods for fabrication of biomimetic scaffolds,as well as their therapeutic efficacy in modulating the BRM of large physiological and pathological bone defects.

关键词： Bone regenerative microenvironemt(BRM) Large physiological bone defects Large pathological bone defects Biomaterial Scaffold

Lymph node metastasis in cancer progression: molecular mechanisms, clinical significance and therapeutic interventions

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Signal Transduction and Targeted Therapy 2023年第10期8卷 4783-4815页

作者： Haoran Ji Chuang Hu Xuhui Yang Yuanhao Liu Guangyu Ji Shengfang Ge xiansong wang Mingsong wang Department of Thoracic Surgery Shanghai Key Laboratory of Tissue EngineeringShanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineShanghai 20o011China Department of Ophthalmology Shanghai Key Laboratory of Orbital Diseases and Ocular OncologyShanghai Ninth People's HospitalShanghai Jiao Tong University School of MedicineShanghai 200025China

dissemination to distant sites through a series of mechanisms,and it has been proved that lymph node metastasis(LNM)is an essential prognostic indicator in many different types of cancer.

关键词： metastasis cancer. Lymph

Intelligent microneedle patch with prolonged local release of hydrogen and magnesium ions for diabetic wound healing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Bioactive Materials 2023年第6期24卷 463-476页

作者： Pei wang Jiayingzi Wu Haiyan Yang Hengke Liu Tianyu Yao Chang Liu Yan Gong Mingsong wang Guangyu Ji Peng Huang xiansong wang Department of Thoracic Surgery Shanghai Key Laboratory of Tissue EngineeringShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai200011China Marshall Laboratory of Biomedical Engineering International Cancer CenterLaboratory of Evolutionary Theranostics(LET)School of Biomedical EngineeringShenzhen University Medical SchoolShenzhen518060China Center of Hydrogen Science Shanghai Jiao Tong UniversityShanghai200240China

Diabetes mellitus,an epidemic with a rapidly increasing number of patients,always leads to delayed wound healing associated with consistent pro-inflammatory M1 polarization,decreased angiogenesis and increased reactive oxygen species(ROS)in the ***,a poly(lactic-co-glycolic acid)(PLGA)-based microneedle patch loaded with magnesium hydride(MgH_(2))(MN-MgH_(2))is manufactured for defeating diabetic *** application of microneedle patch contributes to the transdermal delivery and the prolonged release of MgH_(2) that can generate hydrogen(H_(2))and magnesium ions(Mg^(2+))after reaction with body *** released H_(2) reduces the production of ROS,transforming the pathological microenvironment induced by diabetes ***,the released Mg^(2+)promotes the polarization of pro-healing M2 ***,cell proliferation and migration are improved,and angiogenesis and tissue regeneration are *** intelligent microneedle patch provides a novel way for accelerating wound healing through steadily preserving and releasing of H_(2) and Mg^(2+)locally and sustainably.

关键词： Microneedle patch Magnesium hydride Hydrogen Magnesium ion Diabetic wound healing

Chlorogenic acid supported strontium polyphenol networks ensemble microneedle patch to promote diabetic wound healing

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

Chinese Chemical Letters 2024年第8期35卷 491-497页

作者： Peizhe Li Qiaoling Liu Mengyu Pei Yuci Gan Yan Gong Chuchen Gong Pei wang Mingsong wang xiansong wang Da-Peng Yang Bo Liang Guangyu Ji Research Institute of Plastic Surgery Weifang Medical UniversityWeifang 261000China The Second Affiliated Hospital of Fujian Medical University Quanzhou 362000China Department of Thoracic surgery Shanghai Key Laboratory of Tissue EngineeringShanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China The Third Affiliated Hospital of Xinxiang Medical University Institutes of Health Central PlainClinical Medical Center of Tissue Engineering and RegenerationXinxiang Medical UniversityXinxiang 453003China School of Rehabilitation Science and Engineering University of Health and Rehabilitation SciencesQingdao 266024China

Delayed or non-healing of diabetic wounds is a significant complication,often attributed to high glucose-induced M1 macrophage accumulation,impaired angiogenesis,and reactive oxygen species(ROS)*** this,we introduced a strontium polyphenol network microneedle patch(SrC-MPNs@MN-PP)for percutaneous drug *** patch,formulated with polymer poly(γ-glutamic acid)(γ-PGA)and epsilon-poly-l-lysine(ε-PLL),incorporates strontium polyphenol networks(SrC-MPNs).The release of chlorogenic acid(CGA)from SrC-MPNs not only neutralizes ROS,but strontium ions also foster ***,SrC-MPNs@MN-PP can ameliorate the diabetic wound microenvironment and expedite healing.

关键词： Chlorogenic acid Strontium Microneedle patch Strontium polyphenol network Exudate absorption Diabetic wound healing

乾隆朝民间献书与徽州学人的“不朽”理想——以余元遴进献汪绂遗书为中心的考察

在线全文

学校读者我要写书评

暂无评论

安徽大学学报（哲学社会科学版） 2020年第5期44卷 1-9页

作者：王献松教育部人文社科重点研究基地安徽大学徽学研究中心安徽合肥230039

清代徽州大儒汪绂学宗朱子、著述等身,追求“不朽”是其人生理想。但汪绂生前声名不显,在其死后,弟子余元遴曾多次向朝廷进献汪绂遗书,希望使其人列国史、使其书入《四库》,其间虽经波折,但最终得到安徽学政朱筠的表彰:朱筠为汪绂撰写... 详细信息

清代徽州大儒汪绂学宗朱子、著述等身,追求“不朽”是其人生理想。但汪绂生前声名不显,在其死后,弟子余元遴曾多次向朝廷进献汪绂遗书,希望使其人列国史、使其书入《四库》,其间虽经波折,但最终得到安徽学政朱筠的表彰:朱筠为汪绂撰写《墓表》,从祀汪绂于紫阳书院,并应允誊录汪绂遗书进呈四库全书馆。但经考察,朱筠并未将汪绂遗书进呈四库全书馆,《四库全书》所收汪绂《参〈读礼志疑〉》为翰林院编修励守谦所献。正是由于汪绂自身坚定的学术理想、余元遴献书的不懈坚持、徽州乡绅士子的鼎力帮助以及地方官员的大力表彰等多方面的合力,才最终使汪绂这样一位底层学者得以在地方志、国史、学术史上占据一席之地,最终实现了一个普通人的“不朽”理想。

关键词：徽州汪绂余元遴朱筠《四库全书》 “三不朽” 朱子学

Amorphous calcium magnesium phosphate nanocomposites with superior osteogenic activity for bone regeneration

在线全文

学校读者我要写书评

暂无评论

Regenerative Biomaterials 2021年第6期8卷 249-259页

作者： Yingying Jiang Shuo Tan Jianping Hu Xin Chen Feng Chen Qianting Yao Zhi Zhou xiansong wang Zifei Zhou Yunshan Fan Junjian Liu Yize Lin Lijia Liu Shisheng He Department of Orthopedic Spinal Pain Research InstituteShanghai Tenth People’s HospitalTongji University School of MedicineShanghai 200072China Institute of Translational Medicine Shanghai UniversityShanghai 200444China National Engineering Research Center for Nanotechnology Shanghai 200241China Institute of Functional Nano&Soft Materials(FUNSOM) Jiangsu Key Laboratory for Carbon-Based Functional Materials&DevicesSoochow UniversitySuzhou 215123China Shanghai Key Laboratory of Tissue Engineering Shanghai Ninth People’s HospitalShanghai Jiao Tong University School of MedicineShanghai 200011China

The seek of bioactive materials for promoting bone regeneration is a challenging and longterm *** with inorganic metal ions or drug molecules is considered effective strategies to improve the bioactivity of various existing ***,amorphous calcium magnesium phosphate(ACMP)nanoparticles and simvastatin(SIM)-loaded ACMP(ACMP/SIM)nanocomposites were developed via a simple co-precipitation *** physiochemical property of ACMP/SIM was explored using transmission electron microscope(TEM),Fourier transform infrared spectroscopy(FTIR),powder X-ray diffraction(XRD)and highperformance liquid chromatograph(HPLC),and the role of Mg^(2+) in the formation of ACMP/SIM was revealed using X-ray absorption near-edge structure(XANES).After that,the transformation process of ACMP/SIM in simulated body fluid(SBF)was also tracked to simulate and explore the in vivo mineralization performance of *** find that ACMP/SIM releases ions of Ca^(2+),Mg^(2+)and PO_(4)^(3),when it is immersed in SBF at 37℃,and a phase transformation occurred during which the initially amorphous ACMP turns into self-assembled hydroxyapatite(HAP).Furthermore,ACMP/SIM displays high cytocompatibility and promotes the proliferation and osteogenic differentiation of MC3T3-E1 *** the in vivo studies,lamellar ACMP/SIM/Collagen scaffolds with aligned pore structures were prepared and used to repair a rat defect model in ***/SIM/Collagen scaffolds show a positive effect in promoting the regeneration of calvaria defect after *** bioactive ACMP/SIM nanocomposites are promising as bone repair *** the facile preparation process and superior in vitro/vivo bioactivity,the as-prepared ACMP/SIM would be a potential candidate for bone related biomedical applications.

关键词： biomineralization bone materials synthesize biomaterial-cell

莫洛尼鼠白血病病毒逆转录酶的原核表达纯化及其生物学活性

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

生物工程学报 2008年第5期24卷 903-906页

作者：王贤松马雪梅孙一北京工业大学生命科学与生物工程学院北京100022

利用基因重组技术及简易的纯化方法快速制备莫洛尼鼠白血病病毒逆转录酶(MMLV-RT)。设计带有酶切位点的引物,PCR获得MMLV-rt基因片段;再通过定点突变将目的基因的五个可溶性位点进行突变,测序正确后,插入到表达载体pET15b中构建成重组... 详细信息

利用基因重组技术及简易的纯化方法快速制备莫洛尼鼠白血病病毒逆转录酶(MMLV-RT)。设计带有酶切位点的引物,PCR获得MMLV-rt基因片段;再通过定点突变将目的基因的五个可溶性位点进行突变,测序正确后,插入到表达载体pET15b中构建成重组表达质粒pET15b-MMLV-rt;表达产物的纯品通过金属离子(Ni3+)配体亲和纯化系统得到。用SDS-PAGE分析所纯化产物的大小和纯度,再用RT-PCR对其活性进行鉴定。构建的重组表达质粒pET15b-MMLV-rt经IPTG诱导得到N端带有6His的RT融合蛋白,通过Ni3+的亲和层析得到纯品蛋白,SDS-PAGE分析表明其纯度可达96%,RT-PCR实验表明具有较高的生物学活性。由此得出利用原核表达及简易的纯化系统可获得纯度为96%的逆转录酶纯品,为大规模生产该酶提供了可靠的保证。

关键词：逆转录酶定点突变酶活力

《四库全书总目·性理字训》提要辨正——兼谈《北溪字义》提要之误改

在线全文

学校读者我要写书评

暂无评论

贵州文史丛刊 2021年第2期 66-75页

作者：王献松安徽大学徽学研究中心安徽合肥230039

《四库全书总目》子部儒家类存目部分收录“《性理字训》一卷”,提要于作者项著录为“旧本题宋程端蒙撰,程若庸补辑”,并论定《性理字训》为“村塾学究所讬名”的伪书。文章从“程端蒙、程若庸的学术传承”“《性理字训》文本的流传”... 详细信息

《四库全书总目》子部儒家类存目部分收录“《性理字训》一卷”,提要于作者项著录为“旧本题宋程端蒙撰,程若庸补辑”,并论定《性理字训》为“村塾学究所讬名”的伪书。文章从“程端蒙、程若庸的学术传承”“《性理字训》文本的流传”“《北溪字义》提要的内证”三个方面进行论证并得出结论:《性理字训》确为程端蒙所作,后经程若庸增补为《增广性理字训》,二者皆非伪书。另外,由于《总目》修订者对《性理字训》存在的某种偏见,遂在修订《北溪字义》书前提要的过程中产生了误改现象。而《总目》之所以会对《性理字训》的真伪有这样的误判,这与其在辨伪时常用的“当作品质量与作者地位不相称时,即疑为伪讬”的辨伪方法有关,该辨伪方法需要进行重新检讨。

关键词：《四库全书总目》《性理字训》《北溪字义》辨伪四库学

在线全文

学校读者我要写书评

暂无评论

论《四库全书总目》儒家类文献的著录原则

贵州文史丛刊 2019年第3期 74-84页

作者：王献松安徽大学徽学研究中心

《四库全书总目》子部儒家类著录著作112部,存目著作307部,儒家类著作可划分为'前理学时期''理学时期'两个阶段。前理学时期的著录原则有二:'重古书、古注''重学术流变';理学时期的著录原则有二:'尊崇程朱理学、排斥陆王心学''肯定持论... 详细信息

《四库全书总目》子部儒家类著录著作112部,存目著作307部,儒家类著作可划分为'前理学时期''理学时期'两个阶段。前理学时期的著录原则有二:'重古书、古注''重学术流变';理学时期的著录原则有二:'尊崇程朱理学、排斥陆王心学''肯定持论平允、抨击门户之见'。其中'尊崇程朱理学'的著录原则与《总目》在整体上'重考据轻义理'的精神存在着某种程度的紧张,但二者在本质上并不矛盾,它反映的正是《总目》对历史上的程朱理学的表彰和对现实中的程朱理学的打压,这是《总目》对程朱理学态度的一体两面。

关键词：《四库全书总目》儒家类文献著录理学四库学

论清中期官方对“重考据”学风的营造及其实质

在线全文

学校读者我要写书评

暂无评论

徽学 2018年第1期 162-179页

作者：王献松安徽大学徽学研究中心

乾隆时期程朱理学渐受冷落,考据学代之而起,成为清代'显学'。这一清代学术的考据学转向,与官方对'重考据'学风的营造密不可分,其手段主要是借助对考据学家及其学术的褒奖,为学术界指明新的研究方向——考据学。而尤以编修《四库全书》... 详细信息

乾隆时期程朱理学渐受冷落,考据学代之而起,成为清代'显学'。这一清代学术的考据学转向,与官方对'重考据'学风的营造密不可分,其手段主要是借助对考据学家及其学术的褒奖,为学术界指明新的研究方向——考据学。而尤以编修《四库全书》前后对顾栋高、顾炎武、戴震的褒奖最具代表性,这在很大程度上鼓舞了当时及以后的学者,使之纷纷加入到考据学研究的行列之中。但官方通过编修《四库全书总目》来营造'重考据'学风,并非简单地为了规范清代学术的发展,其背后还蕴含着打压政治朋党、限制言论自由的政治意图。

关键词：清代学术考据学学术风气《四库全书总目》