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World Journal of Gastroenterology 2001年第1期7卷 128-130页

作者： Rong Qian Wu Ying Xin Xu Xu Hua Song Li jun Chen xian jun meng institute of surgical research, general hospital of pla, beijing 100853, china institute of surgical research General Hospital of PLA Beijing 100853 China.

INTRODUCTIONMultiple organ dysfunction syndrome (MODS) isthought to be a frequent consequence of sepsis[1-3].Despite substantial advances in our knowledge and understanding of the basic pathophysiologic mechanisms[4-7... 详细信息

关键词： Animals Cecum Cytokines Endothelium Gene Expression Intercellular Adhesion Molecule-1 Interleukin-1 Interleukin-6 Ligation Liver Mice Punctures RNA, Messenger research Support, Non-U.S. Gov't Sepsis Tumor Necrosis Factor-alpha Vascular Cell Adhesion Molecule-1

Relationship between cytokine mRNA expression and organ damage following cecal ligation and puncture

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World Journal of Gastroenterology 2002年第1期8卷 131-134页

作者： Rong-Qian Wu Ying-Xin Xu Xu-Hua Song Li-jun Chen xian-jun meng institute of surgical research,Chinese pla general hospital,beijing 100853,china institute of surgical research General Hospital of PLA Beijing 100853 China.

AIM: To investigate the role of cytokine gene expression inorgan damage at different tissue sites during ***: Male NIH mice were subjected to cecal ligationand puncture (CLP) or sham operetion ( Sham ). Pro-inflammatory cytokine (TNFα, IL-1β and IL-6) and anti-inflammatory cytokine (IL-4) gene expression in the liverand lung tissue were assessed by RT-PCR. The permeabilityof microvascular and water content in the lungs and liverwere also ***: Significant increase in TNFα, IL-1β and IL-6 geneexpression was observed at 3 and 12 h after CLP both in theliver and lungs ( P＜ 0.01). The level of IL-4 gene expressionwas not changed after CLP in the lungs, but increased at 12h after CLP ( P ＜ 0.01) in the liver tissue. Both the liver andlungs showed a significant increase in microcirculatorypermeability at 12 h after CLP (P＜ 0.01), and the increasein the lungs was higher than that in the liver. The watermaes fractions in the liver ( P＜ 0.05) and lungs ( P＜ 0.01)were increased after CLP, and the increase in the lungshappened earlier and more severely than that in the ***: The inflammatory response in the liver andlungs wes different during sepsis. At the early stage ofsepsis, pro-inflammatory reaction dominates both in theliver and lungs. But at the later stage of sepsis, induction ofcompensatory anti-inflammatory response was seen in theliver but not in the lungs. This difference in situ activity maycontribute to the different vulnerability of organ damageduring sepsis. The strategy of systemic administration ofanti-inflammatory drugs to sepsis should be reconsidered.

关键词：化脓性感染细胞质mRNA 育肠穿刺动物模型育肠结扎术器官损害

sTREM-1 as promising prognostic biomarker for acute-on-chronic liver failure and mortality in patients with acute decompensation of cirrhosis

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World Journal of Gastroenterology 2024年第9期30卷 1177-1188页

作者： Song-Man Yu Hai Li Guo-Hong Deng xian-Bo Wang Xin Zheng Jin-jun Chen Zhong-Ji meng Yu-Bao Zheng Yan-Hang Gao Zhi-Ping Qian Feng Liu Xiao-Bo Lu Yu Shi Jia Shang Ruo-Chan Chen Yan Huang Department of Infectious Diseases Xiangya HospitalCentral South UniversityChangsha 410000Hunan ProvinceChina Department of Gastroenterology Renji HospitalShanghai Jiao Tong University School of MedicineShanghai 200127China Department of Infectious Diseases Southwest HospitalThird Military Medical University(Army Medical University)Chongqing 400038China Center of Integrative Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing 100020China Department of Infectious Diseases Institute of Infection and ImmunologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430020Hubei ProvinceChina Department of Infectious Diseases Nanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Infectious Disease Taihe HospitalHubei University of MedicineShiyan 442009Hubei ProvinceChina Department of Infectious Diseases The Third Affiliated Hospital of Sun Yat-sen UniversityGuangzhou 510630Guangdong ProvinceChina Department of Hepatology The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Liver Intensive Care Unit Shanghai Public Health Clinical CentreFudan UniversityShanghai 200093China Tianjin institute of Hepatology Nankai University Second People's HospitalTianjin 300192China Infectious Disease Center The First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054Xinjiang Uygur Autonomous RegionChina The State Key Laboratory for Diagnosis and Treatment of Infectious Diseases The First Affiliated Hospital of School of MedicineZhejiang UniversityHangzhou 310003Zhejiang ProvineChina Department of Infectious Diseases Henan Provincial People's HospitalZhengzhou 463599Henan ProvineChina Department of Infectious Diseases Hunan Key Laboratory of Viral HepatitisXiangya HospitalCentral South UniversityChangsha 110051Hunan ProvineChina

BACKGROUND Acute decompensation(AD)of cirrhosis is associated with high short-term mortality,mainly due to the development of acute-on-chronic liver failure(ACLF).Thus,there is a need for biomarkers for early and accurate identification of AD patients with high risk of development of ACLF and *** triggering receptor expressed on myeloid cells-1(sTREM-1)is released from activated innate immune cells and correlated with various inflammatory *** To explore the prognostic value of sTREM-1 in patients with AD of *** A multicenter prospective cohort of 442 patients with cirrhosis hospitalized for AD was divided into a study cohort(n=309)and validation cohort(n=133).Demographic and clinical data were collected,and serum sTREM-1 was measured at *** enrolled patients were followed-up for at least 1 *** In patients with AD and cirrhosis,serum sTREM-1 was an independent prognosis predictor for 1-year survival and correlated with liver,coagulation,cerebral and kidney failure.A new prognostic model of AD(P-AD)incorporating sTREM-1,blood urea nitrogen(BUN),total bilirubin(TBil),international normalized ratio(INR)and hepatic encephalopathy grades was established and performed better than the model for end-stage liver disease(MELD),MELD-sodium(MELD-Na),chronic liver failure-consortium(CLIF-C)ACLF and CLIF-C AD ***,sTREM-1 was increased in ACLF and predicted the development of ACLF during first 28-d *** ACLF risk score incorporating serum sTREM-1,BUN,INR,TBil and aspartate aminotransferase levels was established and significantly superior to MELD,MELD-Na,CLIF-C ACLF,CLIF-C AD and P-AD in predicting risk of ACLF *** Serum sTREM-1 is a promising prognostic biomarker for ACLF development and mortality in patients with AD of cirrhosis.

关键词： Soluble triggering receptor expressed on myeloid cell-1 Acute decompensation Cirrhosis Acute-on-chronic liver failure Prognostic biomarker

Inhibition of tumor growth and metastasis with antisense oligonucleotides (Cantide) targeting hTERT in an in situ human hepatocellular carcinoma model

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Acta Pharmacologica Sinica 2005年第6期26卷 762-768页

作者： Ru-xian LIN~2, Chao-wei TUO~3, Qiu-jun LU~2, Wei ZHANG~2, Sheng-qi WANG~(2,4) ~2 beijing institute of Radiation Medicine, beijing 100850, china, ~3 Department of Pathology, pla 202 hospital, Shenyang 110001, china beijing institute of Radiation Medicine Beijing China Department of Pathology PLA 202 Hospital Shenyang China

Aim: To evaluate the in vivo antitumor effects of Cantide and the combined effect with 5-fluorouracil. Methods: An in situ human hepatocellular carcinoma model was established in mice livers orthotopically. Drugs were administered intravenously and tumor sizes were monitored with calipers. plasma alpha-fetoprotein (AFP) were detected by radiation immunoassay. Morphology of tumors was evaluated by hematoxylin-eosin (H&E) staining of histological sections. Human telomerase reverse transcriptase (hTERT) protein levels were detected by Western blotting. Results: Cantide significantly inhibit in situ human hepatocellular carcinoma growth in mice with a 75 and 50 mg kg-1d-1administration of Cantide compared to the saline group in a dose-dependent manner, which included injecting Cantide 25 mg kg-1d-1-75 mg kg-1d-1by iv for 20 d after surgically removing the tumor in liver. Cantide was also found to prevent tumor recurrence in the liver and metastasis in the lung, showing a dose-dependent response. When Cantide was administered by iv combined with 5-fluorouracil, it resulted in a significant reduction in tumor growth compared to either agent alone treatment group. After the treatment with Cantide alone or combined with 5-fluorouracil, plasma AFP concentration decreased in a dose-dependent manner. Conclusion: These results demonstrated that Cantide was an effective antitumor antisense oligonucleotide in vivo and has the potential to be developed into a clinical anti-cancer drug.

关键词： telomerase Cantide hepatocellular carcinoma recurrence neoplasm metastasis

In situ forming injectable MSC-loaded GelMA hydrogels combined with PD for vascularized sweat gland regeneration

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Military Medical research 2024年第1期11卷 152-155页

作者： Enhe Jirigala Bin Yao Zhao Li Yi-Jie Zhang Chao Zhang Li-Ting Liang Fan-Liang Zhang Xing-Yu Yuan xian-Lan Duan Wei Song meng-De Zhang Yi Kong Xiao-Bing Fu Sha Huang research Center for Tissue Repair and Regeneration Medical Innovation Research DepartmentChinese PLA General Hospital and PLA Medical CollegeBeijing 100048China institute of Basic Medical research Inner Mongolia Medical UniversityHohhot 010110China Academy of Medical Engineering and Translational Medicine Tianjin UniversityTianjin 300072China School of Medicine Nankai UniversityTianjin 300071China

Dear Editor,Three dimensional(3D)bioprinted extracellular matrix(ECM)can be used to provide both biochemical and biophysical cues to direct mesenchymal stem cells(MSCs)differentiation,and then differentiated cells were isolated for implantation in vivo using surgical ***,the reduced cell activity after cell isolation from 3D constructs and low cell retention in injured sites limit its application[1].Methacrylated gelatin(GelMA)hydrogel has the advantage of fast crosslinking,which could resemble complex architectures of tissue construct in vivo[2].Here,we adopted a noninvasive bioprinting procedure to imitate the regenerative microenvironment that could simultaneously direct the sweat gland(SG)and vascular differentiation from MSCs and ultimately promote the replacement of glandular tissue in situ(Fig.1a).

关键词： Sweat gland GelMA In situ niche Cell differentiation Tissue incorporation Vascularization

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Screening of hepatocyte proteins binding to NS5ABP37 protein by yeast-two hybrid system

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Journal of Pharmaceutical Analysis 2009年第4期21卷 234-237,251页

作者： Lei Zhang1,Qing-yong Ma1,xian-kui meng1,Kang Li1,jun Cheng21.The First Affiliated hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061 2.institute of Infectious Diseases,beijing Ditan hospital,beijing 100011,china First Affiliated hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China institute of Infectious Diseases Beijing Ditan Hospital Beijing 100011 China

Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in *** We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus(HCV)by cloning the gene of NS5ABP37 protein into pGBKT7,then the recombinant plasmid DNA was transformed into yeast AH109(α type).The transformed yeast AH109 was mated with yeast Y187(α type)containing liver cDNA library plasmid in 2×YPDA *** yeast was plated on synthetic dropout nutrient medium(SD/-Trp-Leu-His-Ade)containing X-α-gal for selection and *** extracting and sequencing of plasmids from positive(blue)colonies,we made a sequence analysis by *** We screened twenty-five proteins binding to NS5ABP37,including Homo sapiens cyclin I(CCNI)gene,Homo sapiens matrix metallopeptidase 25(MMP25)and Homo sapiens talin *** The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of *** the biological function of NS5ABP37 may be associated with glycometabolism,lipid metabolism and apoptosis.

关键词： NS5ABP37 yeast-two hybrid system hepatitis C virus(HCV)

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Detection and evaluation of the mutations of embB gene in etham butol-susceptible and resistant Mycobacterium tuberculos isisolates from china

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中华医学杂志(英文版) 2005年第20期000卷 69-71页

作者： WU Xue-qiong, LIANG Jian-qin, ZHANG jun-xian, LU Yang, LI Hong-min, ZHANG G uang-yu, YAN Guo-rui and DING Bei-chuan Tuberculosis research Laboratory, Tuberculosis Centre, 309th hospital of pla , beijing 100091, china Clinical Laboratory, Thorax Disease hospital of Hebei Province, Shijiazhuang 050 041, china Clinical Laboratory, Thorax Disease hospital of Henan Province, Zhengzhou 450003 , china Clinical Laboratory, research institute of TB Control and Prevention of beijing, beijing 100035, china Tuberculosis research Laboratory Tuberculosis Centre 309th Hospital of PLA Beijing 100091 China Clinical Laboratory Thorax Disease Hospital of Hebei Province Shijiazhuang 050041 China Clinical Laboratory Thorax Disease Hospital of Henan Province Zhengzhou 450003 China Clinical Laboratory Research Institute of TB Control and Prevention of Beijing Beijing 100035 China

Potentiation of the lateral habenula-ventral tegmental area pathway underlines the susceptibility to depression in mice with chronic pain

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Science china(Life Sciences) 2024年第1期67卷 67-82页

作者： Chun-Kui Zhang Pan Wang Yuan-Yuan Ji Jian-Shuai Zhao jun-xiang Gu xian-Xia Yan Hong-Wei Fan Ming-Ming Zhang Yu Qiao Xiao-Die Liu Bao-Juan Li Ming-Hui Wang Hai-Long Dong Hao-Hong Li Peng-Cheng Huang Yun-Qing Li Wu-Gang Hou Jin-Lian Li Tao Chen Department of Anatomy and K.K.Leung Brain research Centre Fourth Military Medical UniversityXi’an 710032China beijing institute of Basic Medical Sciences Beijing 100850China Key Laboratory of Ministry of Public Health for Forensic Science Xi’an Jiaotong UniversityXi’an 710061China Department of Anesthesiology Xijing HospitalFourth Military Medical UniversityXi’an 710032China Department of Neurosurgery the Second Affiliated Hospital of Xian Jiaotong UniversityXi’an 710004China School of Biomedical Engineering Fourth Military Medical UniversityXi’an 710032China Affiliated Mental Health Centre and Hangzhou Seventh People’s hospital Zhejiang University School of MedicineHangzhou 310013China The MOE Frontier research Center of Brain&Brain-machine Integration Zhejiang University School of Brain Science and Brain MedicineHangzhou 310058China Department of Neurology The First Affiliated Hospital of Nanchang UniversityNanchang 330006China Department of Anatomy School of MedicineNorthwest UniversityXi’an 710069China

Chronic pain often develops severe mood changes such as ***,how chronic pain leads to depression remains elusive and the mechanisms determining individuals’responses to depression are largely *** we found that depression-like behaviors could only be observed in 67.9%of mice with chronic neuropathic pain,leaving 32.1%of mice with depression *** determined that the spike discharges of the ventral tegmental area(VTA)-projecting lateral habenula(LHb)glutamatergic(Glu)neurons were sequentially increased in sham,resilient and susceptible mice,which consequently inhibited VTA dopaminergic(DA)neurons through a LHbGlu-VTAGABA-VTADA ***,the LHbGlu-VTADA excitatory inputs were dampened via GABAB receptors in a pre-synaptic *** of LHb-VTA pathway largely affected the development of depressive symptoms caused by chronic *** study thus identifies a pivotal role of the LHb-VTA pathway in coupling chronic pain with depression and highlights the activity-dependent contribution of LHbGlu-to-VTADA inhibition in depressive behavioral regulation.

关键词： chronic pain depression lateral habenula ventral tegmental area dopamine susceptibility

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Expression of vascular endothelial growth factor in cultured human dental follicle cells and its biological roles

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Acta Pharmacologica Sinica 2007年第7期28卷 985-993页

作者： Xue-peng CHEN~2,Hong QIAN~2,jun-jie WU~2,xian-wei MA~3,Ze-xu GU~2,Hai-yan SUN~4,Yin-zhong DUAN~(2,5),Zuo-lin JIN~(2,5)~2 Department of Orthodontics,Qindu Stomatological College,Fourth Military.Medical University,Xi'an 710032,china ~3 institute of Immunology,Second Military Medical University,Shanghai 200433,china,~4 Number 307 hospital of Chinese People's Liberation Army,beijing 100101china Department of Orthodontics Qindu Stomatological College Fourth Military Medical University Xi'an China Instute of Immunology Second Military Medical University Shanghai China Number 307 hospital of chinese People's Liberation Army Beijing China

Aim:To investigate the expression of vascular endothelial growth factor (VEGF)in cultured human dental follicle cells (HDFC),and to examine the roles of VEGF inthe proliferation,differentiation,and apoptosis of HDFC in ***:Immunocytochemistry,ELISA,and RT-PCR were used to detect the expressionand transcription of VEGF in cultured *** dose-dependent and the time-course effect of VEGF on cell proliferation and alkaline phosphatase (ALP) activ-ity in cultured HDFC were determined by MTT assay and colorimetric ALP assay,*** effect of specific mitogen-activated protein kinase (MAPK)inhibitors (PD98059 and U0126) on the VEGF-mediated HDFC proliferation wasalso determined by MTT *** effect of VEGF on HDFC apoptosis wasmeasured by flow ***:VEGF was transcribed and expressed incultured *** at 10-300 μg/L significantly increased HDFC proliferationand ALP activity compared to the *** 1,3,5,or 7 d of stimulation,VEGF induced a significant increase in HDFC proliferation compared with thecorresponding control,while VEGF was effective at increasing ALP activity at theincubation time point of 3,5,or 7 ***98059 and U0126 could attenuate theVEGF-mediated HDFC *** apoptotic cells were observed in theVEGF-treated groups compared to the controls,although the difference was notstatistically ***:VEGF is expressed in cultured HDFC,and ata proper concentration range can stimulate HDFC proliferation,induce HDFC todifferentiate in a"cementoblast/osteoblast"pathway and protect HDFC *** MAPK signaling pathway might be involved in the VEGF-medi-ated HDFC proliferation.

关键词： vascular endothelial growth factor human dental follicle cell proliferation differentiation apoptosis tooth eruption mitogenactivated protein kinase

Characterization of acute-on-chronic liver diseases: A multicenter prospective cohort study

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World Journal of Hepatology 2024年第5期16卷 809-821页

作者： Yuan-Yao Zhang Sen Luo Hai Li Shu-Ning Sun xian-Bo Wang Xin Zheng Yan Huang Bei-Ling Li Yan-Hang Gao Zhi-Ping Qian Feng Liu Xiao-Bo Lu jun-Ping Liu Hao-Tang Ren Yu-Bao Zheng Hua-Dong Yan Guo-Hong Deng Liang Qiao Yan Zhang Wen-Yi Gu Xiao-Mei xiang Yi Zhou Yi-Xin Hou Qun Zhang Yan Xiong Cong-Cong Zou jun Chen Ze-Bing Huang Xiu-Hua Jiang Ting-Ting Qi Yuan-Yuan Chen Na Gao Chun-Yan Liu Wei Yuan Xue Mei Jing Li Tao Li Rong-Jiong Zheng Xin-Yi Zhou jun Zhao Zhong-Ji meng Department of Infectious Disease Institute of Biomedical ResearchRegulatory Mechanism and Targeted Therapy for Liver Cancer Shiyan Key LaboratoryHubei provincial Clinical Research Center for Precise Diagnosis and Treatment of Liver CancerTaihe HospitalHubei University of MedicineShiyan 442000Hubei ProvinceChina Department of Gastroenterology Renji HospitalSchool of MedicineShanghai Jiao Tong UniversityShanghai 200120China Department of Infectious Diseases Southwest HospitalThe Third Military Medical University(Army Medical University)Chongqing 400000China Center of Integrative Medicine Beijing Ditan HospitalCapital Medical UniversityBeijing 100020China Department of Infectious Diseases Institute of Infection and ImmunologyUnion HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430020Hubei ProvinceChina Department of Infectious Diseases Hunan Key Laboratory of Viral HepatitisXiangya HospitalCentral South UniversityChangsha 110051Hunan ProvinceChina Hepatology Unit Department of Infectious DiseasesNanfang HospitalSouthern Medical UniversityGuangzhou 510515Guangdong ProvinceChina Department of Hepatology The First Hospital of Jilin UniversityChangchun 130021Jilin ProvinceChina Department of Liver Intensive Care Unit Shanghai Public Health Clinical CentreFudan UniversityShanghai 200093China Tianjin institute of Hepatology Nankai University Second People's HospitalTianjin 300192China Infectious Disease Center The First Affiliated Hospital of Xinjiang Medical UniversityUrumqi 830054Xinjiang Uygur Autonomous RegionChina Department of Infectious Diseases and Hepatology The Second Hospital of Shandong UniversityJinan 250000Shandong ProvinceChina State Key Laboratory for Diagnosis and Treatment of Infectious Diseases Collaborative Innovation Center for Diagnosis and Treatment of Infectious DiseaseThe First Affiliated HospitalZhejiang University School of MedicineHangzhou 310000Zhejiang ProvinceChina Department of Infectious Diseases The Thir

BACKGROUND Acute-on-chronic liver disease(AoCLD)accounts for the majority of patients hospitalized in the Department of Hepatology or Infectious *** To explore the characterization of AoCLD to provide theoretical guidance for the accurate diagnosis and prognosis of *** Patients with AoCLD from the Chinese Acute-on-Chronic Liver Failure(ACLF)study cohort were included in this *** clinical characteristics and outcomes,and the 90-d survival rate associated with each clinical type of AoCLD were analyzed,using the Kaplan-Meier method and the log-rank *** A total of 3375 patients with AoCLD were enrolled,including 1679(49.7%)patients with liver cirrhosis acute decompensation(LC-AD),850(25.2%)patients with ACLF,577(17.1%)patients with chronic hepatitis acute exacer-bation(CHAE),and 269(8.0%)patients with liver cirrhosis active phase(LC-A).The most common cause of chronic liver disease(CLD)was HBV infection(71.4%).The most common precipitants of AoCLD was bacterial infection(22.8%).The 90-d mortality rates of each clinical subtype of AoCLD were 43.4%(232/535)for type-C ACLF,36.0%(36/100)for type-B ACLF,27.0%(58/215)for type-A ACLF,9.0%(151/1679)for LC-AD,3.0%(8/269)for LC-A,and 1.2%(7/577)for *** HBV infection is the main cause of CLD,and bacterial infection is the main precipitant of *** most common clinical type of AoCLD is *** diagnosis and timely intervention are needed to reduce the mortality of patients with LC-AD or ACLF.

关键词： Acute-on-chronic liver disease Acute-on-chronic liver failure Liver cirrhosis Clinical features Prognosis