Relationship between cytokine mRNA expression and organ damage following cecal ligation and puncture
Relationship between cytokine mRNA expression and organ damage following cecal ligation and puncture作者机构：Institute of surgical Research General Hospital of PLA Beijing 100853 China.
出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)
年 卷 期：2002年第8卷第1期
页 面：131-134页
核心收录：
学科分类：1002[医学-临床医学] 100210[医学-外科学(含：普外、骨外、泌尿外、胸心外、神外、整形、烧伤、野战外)] 10[医学]
基 金：the National Natural Science Foundation of China No.39870796
主 题：化脓性感染 细胞质mRNA 育肠穿刺 动物模型 育肠结扎术 器官损害
摘 要：AIM: To investigate the role of cytokine gene expression inorgan damage at different tissue sites during ***: Male NIH mice were subjected to cecal ligationand puncture (CLP) or sham operetion ( Sham ). Pro-inflammatory cytokine (TNFα, IL-1β and IL-6) and anti-inflammatory cytokine (IL-4) gene expression in the liverand lung tissue were assessed by RT-PCR. The permeabilityof microvascular and water content in the lungs and liverwere also ***: Significant increase in TNFα, IL-1β and IL-6 geneexpression was observed at 3 and 12 h after CLP both in theliver and lungs ( P 0.01). The level of IL-4 gene expressionwas not changed after CLP in the lungs, but increased at 12h after CLP ( P 0.01) in the liver tissue. Both the liver andlungs showed a significant increase in microcirculatorypermeability at 12 h after CLP (P 0.01), and the increasein the lungs was higher than that in the liver. The watermaes fractions in the liver ( P 0.05) and lungs ( P 0.01)were increased after CLP, and the increase in the lungshappened earlier and more severely than that in the ***: The inflammatory response in the liver andlungs wes different during sepsis. At the early stage ofsepsis, pro-inflammatory reaction dominates both in theliver and lungs. But at the later stage of sepsis, induction ofcompensatory anti-inflammatory response was seen in theliver but not in the lungs. This difference in situ activity maycontribute to the different vulnerability of organ damageduring sepsis. The strategy of systemic administration ofanti-inflammatory drugs to sepsis should be reconsidered.
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