Screening of hepatocyte proteins binding to NS5ABP37 protein by yeast-two hybrid system

作     者：Lei Zhang1,Qing-yong Ma1,Xian-kui Meng1,Kang Li1,Jun Cheng21.The First Affiliated Hospital,Medical School of Xi’an Jiaotong University,Xi’an 710061 2.Institute of Infectious Diseases,Beijing Ditan Hospital,Beijing 100011,China 

作者机构：First Affiliated Hospital Medical School of Xi'an Jiaotong University Xi'an 710061 China Institute of Infectious Diseases Beijing Ditan Hospital Beijing 100011 China 

出 版 物：《Journal of Pharmaceutical Analysis》 (药物分析学报（英文版）)

年 卷 期：2009年第21卷第4期

页      面：234-237,251页

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

主　　题：NS5ABP37 yeast-two hybrid system hepatitis C virus(HCV) 

摘      要：Objective To investigate the biological function of NS5ABP37 and to look for proteins interacting with NS5ABP37 protein in *** We constructed bait plasmid expressing NS5ABP37 protein of hepatitis C virus(HCV)by cloning the gene of NS5ABP37 protein into pGBKT7,then the recombinant plasmid DNA was transformed into yeast AH109(α type).The transformed yeast AH109 was mated with yeast Y187(α type)containing liver cDNA library plasmid in 2×YPDA *** yeast was plated on synthetic dropout nutrient medium(SD/-Trp-Leu-His-Ade)containing X-α-gal for selection and *** extracting and sequencing of plasmids from positive(blue)colonies,we made a sequence analysis by *** We screened twenty-five proteins binding to NS5ABP37,including Homo sapiens cyclin I(CCNI)gene,Homo sapiens matrix metallopeptidase 25(MMP25)and Homo sapiens talin *** The yeast-two hybrid system is an effective method for identifying hepatocyte proteins interacting with NS5ABP37 of *** the biological function of NS5ABP37 may be associated with glycometabolism,lipid metabolism and apoptosis.