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Trophic Mode-Dependent Proteomic Analysis Reveals Functional Significance of Light- Independent Chlorophyll Synthesis in Synechocystis sp. PCC 6803

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Molecular Plant 2017年第1期10卷 73-85页

作者： Longfa Fang Haitao Ge xiahe huana Ye Liu Min Lu Jinlong Wang Weiyang Chen Wu Xu Yingchun Wang State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental Biology Chinese Academy of Sciences No.1 West Beichen Road Beijing 100101 China Department of Chemistry University of Louisiana at Lafayette Lafayette LA 70504 USA These authors contributed equally to this article

The photosynthetic model organism Synechocystis sp. PCC 6803 can grow in different trophic modes, depending on the availability of light and exogenous organic carbon source. However, how the protein pro- file changes to facilitate the cells differentially propagate in different modes has not been comprehensively investigated. Using isobaric labeling-based quantitative proteomics, we simultaneously identified and quantified 45% Synechocystis proteome across four different trophic modes, i.e., autotrophic, heterotro- phic, photoheterotrophic, and mixotrophic modes. Among the 155 proteins that are differentially expressed across four trophic modes, proteins involved in nitrogen assimilation and light-independent chlorophyll synthesis are dramatically upregulated in the mixotrophic mode, concomitant with a dramatic increase of PII phosphorylation that senses carbon and nitrogen assimilation status. Moreover, functional study us- ing a mutant defective in light-independent chlorophyll synthesis revealed that this pathway is important for chlorophyll accumulation under a cycled light/dark illumination regime, a condition mimicking day/night cycles in certain natural habitats. Collectively, these results provide the most comprehensive information on trophic mode-dependent protein expression in cyanobacterium, and reveal the functional significance of light-independent chlorophyll synthesis in trophic growth.

关键词： proteomics phosphorylation Synechocystis light-independent chlorophyll synthesis nitrogenassimilation

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Non-transcriptional regulatory activity of SMAX1 and SMXL2 mediates karrikin-regulated seedling response to red light in Arabidopsis

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Molecular Plant 2024年第7期17卷 1054-1072页

作者： Wenwen Chang Qiao Qiao Qingtian Li Xin Li Yanyan Li xiahe Huang Yingchun Wang Jiayang Li Bing Wang Lei Wang Key Laboratory of Seed Innovation Center for Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuangHebei050021China Key Laboratory of Seed Innovation Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing100101China University of Chinese Academy of Sciences Beijing 100049China Yazhouwan National Laboratory SanyaHainan 572024China

Karrikins and strigolactones govern plant development and environmental responses through closely related signaling *** transcriptional repressor proteins SUPPRESSOR OF MAX21(SMAX1),SMAX1-like2(SMXL2),and D53-like SMXLs mediate karrikin and strigolactone signaling by directly binding downstream genes or byinhibiting the activities of transcription *** this study,we characterized the non-transcriptional regulatory activities of SMXL proteins in *** discovered that SMAX1 and SMXL2 with mutations in their ethylene-responsefactor-associated amphiphilic repression(EAR)motif had undetectable or weak transcriptional repression activities but still partially rescued the hypocotyl elongation defects and fully reversed the cotyledon epinasty defects of the smax1 smxl2 ***1 and SMXL2 directly interact with PHYTOCHROME INTERACTION FACTOR4(PIF4)and PIF5 to enhance their protein stability by interacting with phytochrome B(phyB)and suppressing the association of phyB with PIF4 and *** karrikin-responsive genes were then identified by treatment with GR24ent-ssa,GR24 analog showing karrikin ***,INDOLE-3-ACETIC ACID INDUCIBLE 29(IAA29)expression was repressed by GR24^(ent-5D)streatment in a PIF4-and PIF5-dependent and EARindependent manner,whereas KARRIKIN UPREGULATED F-BOX 1(KUF1)expression was induced in a PIF4-and PIF5-independent and EAR-dependent ***,the non-transcriptional regulatory activity of SMAX1,which is independent of the EAR motif,had a global effect on gene *** together,these results indicate that non-transcriptional regulatory activities of SMAX1 and SMXL2 mediate karrikin-regulated seedling response to red light.

关键词： karrikin signaling SMAX1 and SMXL2 non-transcriptional repression activity PIF4 and PIF5 gene expression red light

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GreenPhos,a universal method for in-depth measurement of plant phosphoproteomes with high quantitative reproducibility

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Molecular Plant 2024年第1期17卷 199-213页

作者： Xiaoxiao Duan Yuanya Zhang xiahe Huang Xiao Ma Hui Gao Yan Wang Zhen Xiao Chengcheng Huang Zhongshu Wang Bolong Li Wenqiang Yang Yingchun Wang State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBejing 100101China University of Chinese Academy of Sciences Bejing 100049China Key Laboratory of Photobiology Instute of BotanyChinese Academy of SciencesBejing 100093China

Protein phosphorylation regulates a variety of important cellular and physiological processes in ***-depth profiling of plant phosphoproteomes has been more technically challenging than that of animal *** is largely due to the need to improve protein extraction efficiency from plant cells,which have a dense cell wall,and to minimize sample loss resulting from the stringent sample clean-up steps required for the removal of a large amount of biomolecules interfering with phosphopeptide purification and mass spectrometry *** this end,we developed a method with a streamlined workflow for highly efficient purification of phosphopeptides from tissues of various green organisms including Arabidopsis,rice,tomato,and Chlamydomonas reinhardtii,enabling in-depth identification with high quantitative reproducibility of about 11000 phosphosites,the greatest depth achieved so far with single liquid chromatography-mass spectrometry(LC-MS)runs operated in a data-dependent acquisition(DDA)*** mainstay features of the method are the minimal sample loss achieved through elimination of sample clean-up before protease digestion and of desalting before phosphopeptide enrichment and hence the dramatic increases of time-and *** method,named GreenPhos,combined with single-shot LC-MS,enabled in-depth quantitative identification of Arabidopsis phosphoproteins,including differentially phosphorylated spliceosomal proteins,at multiple time points during salt stress and a number of kinase substrate *** is expected to serve as a universal method for purification of plant phosphopeptides,which,if samples are further fractionated and analyzed by multiple LC-MS runs,could enable measurement of plant phosphoproteomes with an unprecedented depth using a given mass spectrometry technology.

关键词： plant phosphoproteomics phosphopeptides LC–MS Arabidopsis salt stress

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Long-chain acyl-CoA synthetase regulates systemic lipid homeostasis via glycosylation-dependent lipoprotein production

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Life Metabolism 2024年第2期3卷 38-54页

作者： Jie Li Yue Dong Tianxing Zhou He Tian xiahe Huang Yong Q.Zhang Yingchun Wang Sin Man Lam Guanghou Shui State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100101China Lipidall Technologies Company Limited ChangzhouJiangsu 213000China

Interorgan lipid transport is crucial for organism development and the maintenance of physiological ***,we demonstrate that Drosophila long-chain acyl-CoA synthetase(dAcsl),which catalyzes the conversion of fatty acids into acyl-coenzyme As(acyl-CoAs),plays a critical role in regulating systemic lipid *** deficiency in the fat body led to the ectopic accumulation of neutral lipids in the gut,along with significantly reduced lipoprotein contents in both the fat body and *** aberrant phenotypes were rescued by fat body-specific overexpression of apolipophorin.A multi-omics investigation comprising lipidomics,metabolomics,and proteomics in conjunction with genetic screening revealed that glycosylation processes were suppressed in dAcsl knockdown *** of CG9035,human ortholog of which is implicated in the congenital disorder of glycosylation,ame-liorated gut lipid accumulation in *** lipoprotein glycosylation led to accelerated proteasome-related degradation and induced ER stress in dAcsl knockdown flies,impairing lipoprotein release into the circulation which compromised interorgan lipid transport between the fat body and the *** of ubiquitin-proteasome-dependent degradation alleviated the phenotype of gut ectopic fat accumulation in dAcsl knockdown ***,we verified that ACSL4,the human homolog of dAcsl,also regulated lipoprotein levels in HepG2 cells,indicating that the role of dAcsl in modulating lipoprotein secretion and systemic lipid homeostasis is possibly conserved in humans.

关键词： lipid homeostasis apolipoprotein glycosylation lipidomics metabolomics proteomics

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Computational insights into different regioselectivities in the Ir-porphyrin-catalyzed C–H insertion reaction of quinoid carbene

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Organic Chemistry Frontiers 2022年第4期9卷 1143-1151页

作者： xiahe Chen Chaoqin Cao Yun-Fang Yang Yuan-Bin She College of Chemical Engineering Zhejiang University of TechnologyHangzhouZhejiang 310014China

The mechanisms and regioselectivities of the Ir-porphyrin-catalyzed C–H insertion reaction of quinoid carbene(QC)were investigated by density functional theory(DFT)*** competing catalytic cycles were identified as the hydrogen-atom abstraction by quinoid oxygen(HAA-O)and hydrogen-atom abstraction by carbene carbon(HAA-C)catalytic cycles.

关键词： porphyrin insertion catalyzed

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全程多学科营养模式在老年患者髋部手术中的应用研究

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中国现代医生 2024年第22期62卷 17-21页

作者：陈夏荷吕杨训陈琦凡林洲廖飞飞温州市中心医院骨科浙江温州325000

目的探讨全程多学科营养模式在老年患者髋部手术中的应用效果。方法选取2021年10月至2023年12月于温州市中心医院行髋部手术的老年患者90例,根据随机数字表法将其分为对照组和研究组,每组各45例。对照组患者应用饮食管理模式,研究组患... 详细信息

目的探讨全程多学科营养模式在老年患者髋部手术中的应用效果。方法选取2021年10月至2023年12月于温州市中心医院行髋部手术的老年患者90例,根据随机数字表法将其分为对照组和研究组,每组各45例。对照组患者应用饮食管理模式,研究组患者应用全程多学科营养模式。比较两组患者的恢复情况、营养状况、生活质量、髋关节功能和满意度。结果研究组患者的住院时间、骨折愈合时间均显著短于对照组(P<0.05)。干预后,两组患者的前白蛋白(prealbumin,PA)、白蛋白(albumin,Alb)、血红蛋白(hemoglobin,Hb)、健康调查量表36(36-item short form health survey,SF-36)各维度评分、Harris髋关节评分均显著高于本组干预前(P<0.05),且研究组患者的PA、Alb、Hb、SF-36各维度评分、Harris髋关节评分均显著高于对照组(P<0.05)。研究组患者的满意度显著高于对照组(χ^(2)=5.075,P=0.024)。结论老年患者髋部手术中应用全程多学科营养模式可改善其营养状态,提升生活质量,增强髋关节功能,有利于术后恢复。

关键词：多学科团队营养模式髋部手术

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基于车路协同大流量高速公路多车道管控策略研究

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山西建筑 2024年第18期50卷 116-121页

作者：任延凯俞顺刘正伟吕夏合徐亚辉河南交通投资集团有限公司河南郑州450000 河南交投中原高速郑洛建设有限公司河南郑州450000 河南中原高速公路股份有限公司航空港分公司河南郑州450000 郑州市规划勘测设计研究院有限公司河南郑州450000 河南省交通规划设计研究院股份有限公司河南郑州450000

随着信息技术的飞速发展,车路协同技术成为发展智慧公路的重要技术手段。应用主动式交通管理以提供更加实时有效的信息已成为行业新共识。通过对大流量交通流场景下的高速公路交通特征及道路通行能力进行分析,提出基于车路协同大流量影... 详细信息

随着信息技术的飞速发展,车路协同技术成为发展智慧公路的重要技术手段。应用主动式交通管理以提供更加实时有效的信息已成为行业新共识。通过对大流量交通流场景下的高速公路交通特征及道路通行能力进行分析,提出基于车路协同大流量影响下的多车道级控制策略。本次仿真实验利用VISSIM软件二次开发搭建高速公路主线大流量交通场景,考虑车路协同场景下车路信息交互,搭建车辆跟驰模型及其换道模型。通过分析河南省高速公路交通数据,建立微观交通仿真环境,对不同管控措施下道路占有率、平均延误时间、平均车辆速度以及道路通行时间等交通流特征进行模型仿真分析。结果表明:相较于无管控道路状态,文中提出的面向车路协同环境的动态控制车辆速度以及硬路肩开放的管控措施能够更加有效地消除高速公路大流量瓶颈区域拥堵,提升道路通行效率及其道路安全性。

关键词：车路协同高速公路动态限速跟驰模型主动式交通管控

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OsMPK4 promotes phosphorylation and degradation of IPA1 in response to salt stress to confer salt tolerance in rice

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Journal of Genetics and Genomics 2022年第8期49卷 766-775页

作者： Meiru Jia Nan Luo Xiangbing Meng Xiaoguang Song Yanhui Jing Liquan Kou Guifu Liu xiahe Huang Yingchun Wang Jiayang Li Bing Wang Hong Yu State Key Laboratory of Plant Genomics and National Center for Plant Gene Research Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China State Key Laboratory of Molecular Developmental Biology Institute of Genetics and Developmental BiologyInnovation Academy for Seed DesignChinese Academy of SciencesBeijing 100101China

Salt stress adversely affects plant growth,development,and crop ***(Oryza sativa L.)is one of the most salt-sensitive cereal crops,especially at the early seedling ***-activated protein kinase(MAPK/MPK)cascades have been shown to play critical roles in salt response in ***,the roles of the MPK cascade signaling in rice salt response and substrates of Os MPK remain largely ***,we report that the salt-induced Os MPK4-Ideal Plant Architecture 1(IPA1)signaling pathway regulates the salt tolerance in *** salt stress,Os MPK4 could interact with IPA1 and phosphorylate IPA1 at Thr180,leading to degradation of *** evidence shows that IPA1 is a negative regulator of salt tolerance in rice,whereas Os MPK4 promotes salt response in an IPA1-dependent *** together,our results uncover an Os MPK4-IPA1 signal cascade that modulates the salt stress response in rice and sheds new light on the breeding of salt-tolerant rice varieties.

关键词： Oryza sativa MPK OsMPK4 IPA1 Salt tolerance

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Microstructure induced galvanic corrosion evolution of SAC305 solder alloys in simulated marine atmosphere

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Journal of Materials Science & Technology 2020年第16期47卷 40-53页

作者： Mingna Wang Chuang Qiao Xiaolin Jiang Long Hao xiahe Liu Department of Physics Hebei Normal University of Science&TechnologyQinhuangdao 066004China School of Metallurgy Northeastern UniversityShenyang 110819China Environmental Corrosion Centre of Materials Institute of Metal ResearchChinese Academy of SciencesShenyang 110016China School of Material Science and Engineering University of Science and Technology of ChinaHefei 230026China

Motivated by the increasing use of Sn-3.0 Ag-0.5 Cu(SAC305)solder in electronics worked in marine atmospheric environment and the uneven distribution of Ag3Sn and Cu6Sn5 intermetallic compounds(IMCs)inβ-Sn matrix,comb-like electrodes have been designed for in-situ EIS measurements to study the microstructure induced galvanic corrosion evolution of SAC305 solder in simulated marine atmosphere with high-temperature and *** indicate that in-situ EIS measurement by comb-like electrodes is an effective method for corrosion evolution behavior study of SAC305 ***,the galvanic effect between Ag3Sn IMCs andβ-Sn matrix can aggravate the corrosion of both as-received and furnace-cooled SAC305 solder as the exposure time proceeds in spite of the presence of corrosion product *** corrosion can be preferentially found on furnace-cooled SAC305 with larger Ag3Sn grain ***,the generated inner stress during phases transformation process with Sn3O(OH)2Cl2 as an intermediate and the possible hydrogen evolution at local acidified sites are supposed to be responsible for the loose,porous,cracked,and non-adherent corrosion product *** findings clearly demonstrate the corrosion acceleration behavior and mechanism of SAC305 solder,and provide potential guidelines on maintenance of microelectronic devices for safe operation and longer in-service duration.

关键词： SAC305 solder Marine atmosphere Galvanic corrosion In-situ EIS Comb-like electrode

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An engineered platform for reconstituting functional multisubunit SCF E3 ligase in vitro

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Molecular Plant 2022年第8期15卷 1285-1299页

作者： Huihui Liu Simiao Liu Hong Yu xiahe Huang Yingchun Wang Liang Jiang Xiangbing Meng Guifu Liu Mingjiang Chen Yanhui Jing Feifei Yui Bing Wang Jiayang Li State Key Laboratory of Plant Genomics and National Center for Plant Gene Research(Beijing) Institute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing 100101China University of Chinese Academy of Sciences Beijing 100049China

Multisubunit SKP1/Cullin1/F-box(SCF)E3 ligases play essential roles in regulating the stability of crucial regulatory factors and controlling growth and development in *** E3 ligase activity in vitro is important forexploring the molecular mechanism of protein ***,in vitro ubiquitination assay systems for multisubunit E3 ligases remain difficult to achieve,especially in plants,mainly owing to difficulties in achieving active components of multisubunit E3 ligases with high purity and characterizing specific E2 and E3 *** this study,we characterized components of the rice ScFDiwARF3(SCFDs)E3 ligase,screened the coordinated E2,and reconstituted active ScFD3 E3 ligase in *** further engineered SCFD3 E3 ligase using a fused SKP1-Cullin1-RBX1(eSCR)protein and found that both the wild-type SCFD3 E3 ligase and the engineered SCFD3 E3 ligase catalyzed ubiquitination of the substrate D53,which is the key transcriptional repressor in strigolactone ***,we replaced D3 with other F-box proteins from rice and humans and reconstituted active escF E3 ligases,including escFaID2,escFBxL1s,and escFcDC4 E3 *** work reconstitutes functional SCF E3 ligases in vitro and generates an engineered system with interchangeable F-box proteins,providing a powerful platform for studying the mechanisms of multisubunit SCF E3 ligases in eukaryotes.

关键词： rice SCF E3 ligase ubiquitination strigolactones DWARF53 Sic1

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