Non-transcriptional regulatory activity of SMAX1 and SMXL2 mediates karrikin-regulated seedling response to red light in Arabidopsis
作者机构：Key Laboratory of Seed InnovationCenter for Agricultural Resources ResearchInstitute of Genetics and Developmental BiologyChinese Academy of SciencesShijiazhuangHebei050021China Key Laboratory of Seed InnovationInstitute of Genetics and Developmental BiologyThe Innovative Academy for Seed DesignChinese Academy of SciencesBeijing100101China University of Chinese Academy of SciencesBeijing 100049China Yazhouwan National LaboratorySanyaHainan 572024China
出 版 物：《Molecular Plant》 (分子植物（英文版）)
年 卷 期：2024年第17卷第7期
页 面：1054-1072页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：the National Natural Science Foundation of China(32170320,32122012,and 32270327) the Hebei Natural Science Foundation(C2022503003) the Youth Innovation Promotion Association of the Chinese Academy of Sciences(Y2023025)
主 题：karrikin signaling SMAX1 and SMXL2 non-transcriptional repression activity PIF4 and PIF5 gene expression red light
摘 要：Karrikins and strigolactones govern plant development and environmental responses through closely related signaling *** transcriptional repressor proteins SUPPRESSOR OF MAX21(SMAX1),SMAX1-like2(SMXL2),and D53-like SMXLs mediate karrikin and strigolactone signaling by directly binding downstream genes or byinhibiting the activities of transcription *** this study,we characterized the non-transcriptional regulatory activities of SMXL proteins in *** discovered that SMAX1 and SMXL2 with mutations in their ethylene-responsefactor-associated amphiphilic repression(EAR)motif had undetectable or weak transcriptional repression activities but still partially rescued the hypocotyl elongation defects and fully reversed the cotyledon epinasty defects of the smax1 smxl2 ***1 and SMXL2 directly interact with PHYTOCHROME INTERACTION FACTOR4(PIF4)and PIF5 to enhance their protein stability by interacting with phytochrome B(phyB)and suppressing the association of phyB with PIF4 and *** karrikin-responsive genes were then identified by treatment with GR24ent-ssa,GR24 analog showing karrikin ***,INDOLE-3-ACETIC ACID INDUCIBLE 29(IAA29)expression was repressed by GR24^(ent-5D)streatment in a PIF4-and PIF5-dependent and EARindependent manner,whereas KARRIKIN UPREGULATED F-BOX 1(KUF1)expression was induced in a PIF4-and PIF5-independent and EAR-dependent ***,the non-transcriptional regulatory activity of SMAX1,which is independent of the EAR motif,had a global effect on gene *** together,these results indicate that non-transcriptional regulatory activities of SMAX1 and SMXL2 mediate karrikin-regulated seedling response to red light.
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