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Hetero-bimetallic transition metal-substituted Krebs-type polyoxometalate with N-chelating ligand as anticancer agents

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钨科技(英文) 2023年第2期5卷 225-234页

作者： Han-Zhi Zhang Hong-xi. Zhao Wen-Hui Chang xi.-Yuan Liu Peng Chen Ai-Qin Yu Aadil Nabi Chishti Yu-Zhen Zhang Lu-Bin ni xi.Qing Wang Yong-Ge wei School of Chemistry and Chemical Engineering Yangzhou UniversityYangzhou 225002China College of Bioscience and Biotechnology Yangzhou UniversityYangzhou 225009China School of Chemistry and Chemical Engineering Guangxi Minzu UniversityNo.158Daxue West RoadNanning 530006China Key Lab of Organic Optoelectronics and Molecular Engineering of Ministry of Education Department of ChemistryTsinghua UniversityBeijing 100084China

A novel hetero-bimetallic transition metal(Sb^(Ⅴ)/Mn^(Ⅱ))-substituted Krebs-type polyoxometalate(POM)with N-chelating ligand H_(4)Na_(4)[Mn_(4)(H_(2)O)_(4)(trz)_(2)(SbO_(2))_(2)(SbW_(9)O_(33))_(2)]-20H_(2)O(Sb_(2)Mn_(2))(1)(trz=1,2,4-triazole)built from trilacunary Keggin POM unitα-B-[SbW_(9)O_(33)]^(9-){SbW_(9)]has been synthesized and characterized by single-crystal/powder X-ray diffraction and a wide range of analytical methods,including powder X-ray diffraction,Fourier transform infrared spectroscopy,Raman,ultraviolet-visible spectroscopy,electrospray ionization-mass spectroscopy,and thermogravimetric *** further investigate the antitumor activity of the(Sb_(2)Mn_(2))(1)in human gastric cancer Human Gastric Adenocarcinoma Cells and Human Gastric Cancer Cell lines,in vitro cytotoxi.ity assay and flow cytometry analysis was *** results indicated that the IC_(50) values of(Sb_(2)Mn_(2))(1)on Human Gastric Adenocarcinoma(AGS)and Human Gastric Cancer(BGC-823)Cell Lines were 1.86±0.05μmol·L^(-1) and 14.61±0.55μmol·L^(-1),respectively.(Sb_(2)Mn_(2))(1)also exhibited high cytotoxi.ity on Human Gastric Cancer Cells and could induce cell apoptosis by inhibiting S and G_(2)/M cell ***,the result not only shows that this new POM-based inorganic-organic hybrid compound has high selectivity and low toxi.ity,but also provides an effective method for the development of potential transition metal-substituted POMs based antitumor drugs.

关键词： Polyoxometalate Inorganic-organic hybrid material-In vitro cytotoxi.ity Anticancer agent

Colonic vitamin D receptor expression is inversely associated with disease activity and jumonji domain-containing 3 in active ulcerative colitis

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World Journal of Gastroenterology 2020年第46期26卷 7352-7366页

作者： Hong-Qian Wang Wen-Hui Zhang Ya-Qi Wang xi.o-Pan Geng Ming-wei Wang Yuan-Yuan Fan Jing Guan Ji-Long Shen xi.Chen Department of Gastroenterology The First Affiliated Hospital of Anhui Medical UniversityHefei 230022Anhui ProvinceChina Department of Pathogen Biology Anhui Medical UniversityHefei 230032Anhui ProvinceChina

BACKGROUND The expression of jumonji domain-containing 3(Jmjd3)and trimethylated H3 lysine 27(H3K27me3)in active ulcerative colitis(UC)and the correlation between vitamin D receptor(VDR)and the Jmjd3 pathway are *** To study the relationship between VDR,Jmjd3 and H3K27me3 in patients with active *** One hundred patients with active UC and 56 healthy controls were enrolled in this *** patients with active UC were divided into groups according to mild(n=29),moderate(n=32)and severe(n=29)disease activity based on the modified Mayo *** D levels were measured by *** mucosal tissues from UC patients and controls were collected by *** expression of VDR,Jmjd3 and H3K27me3 in the intestinal mucosa was determined by immunohistochemistry *** Patients with active UC had lower levels of serum vitamin D(13.7±2.8 ng/mL,Png/mL).In the UC cohort,serum vitamin D level was negatively correlated with disease activity(r=-0.323,P=0.001).VDR expression in the mucosa of UC patients was reduced compared to that in normal tissues(Pxi.al to the anus(defined as UC normal)(Pnificantly increased in UC patients(Pme3 expression in UC patients was significantly down-regulated when compared with normal tissues(Pme3(r=-0.341,P=0.002),while VDR level was positively correlated with H3K27me3(r=0.473,P<0.001).CONCLUSION Serum vitamin D and VDR were inversely correlated with

关键词： Vitamin D Ulcerative colitis Disease activity Vitamin D receptor Jumonji domain-containing 3 Trimethylated H3 lysine 27

Thermal Aspects for Face Grinding of Ceramics

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厦门大学学报（自然科学版） 2002年第S1期41卷 123-124页

作者： SHEN Jian-yun, ZEng wei-ming, HUAng Hui, XU xi.peng (College of mechanical Engineering and Automation, Huaqiao University , Quanzhou 362011, China)

To meet the increasing demand on the quality and co st of precision structural components, extensive studies on high efficiency and pr ecision machining of ceramic materials, including face grinding, have been condu cted over the past several years. However, there are few reports about the mecha nism in face grinding of ceramics, especially the thermal aspects during the gri nding process. In the present study, experiments of face grinding two typical ce ramics were carried out to study themal aspects at the wheel-workpiece contact zone. The present investigation was undertaken to experimentally study the energy part ition to workpiece and thermal characteristics during face grinding of two typic al ceramics. For this purpose, grinding temperature distribution in the ceramics workpiece was calculated with the classic moving heat source theory. The grindi ng temperature at the wheel-workpiece contact zone was measured under different machining conditions using a grindable foil thermocouple. Combining the analyti cal results and the experimental result for temperatures, the mechanism of energ y partition in face grinding of ceramic materials was discussed. According to the experimental results and the comparison of theoretic and measur ed temperature profiles, temperature characteristics and mechanism of energy par tition in face grinding of ceramic materials were discussed. Temperature rise in the workpiece and energy partition during the grinding process were found to be derictly related to the machining parameters and the mechanism of mateirial rem oval. The increasing of either v w or a p can lead to an increase of temperatu re. The temperature rise in Si 3N 4 is much lower than that in ZrO 2 under th e same condition, which might be attributed to the different material removal me chanisms. Both temperatures in dry grinding and in wet grinding are not high eno ugh to cause thermal damage to ceramic materials. The value of energy partition increases with the increa

关键词： ceramic face grinding grinding temperature

Micro/nano-mechanical behaviors of individual FCC,BCC and FCC/BCC interphase in a high-entropy alloy

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Journal of Materials Science & Technology 2022年第19期114卷 102-110页

作者： wei Zhang Zhichao Ma Chaofan Li Chaowei Guo Dongni Liu Hongwei Zhao Luquan Ren School of mechanical and Aerospace Engineering Jilin UniversityChangchun 130025China Key Laboratory of CNC Equipment Reliability Ministry of EducationJilin UniversityChangchun 130025China State Key Laboratory for mechanical Behavior of Materials Xi’an Jiaotong UniversityXi’an 710049China Key Laboratory of Bionic Engineering Ministry of Education Jilin UniversityChangchun 130025China weihai Institute for Bionics Jilin UniversityWeihai 264207China

Here,a systematic investigation was made on the interphase strengthening effects induced superior me-chanical performances of multiphase high-entropy alloys(HEAs)at micro/nano-scale,compared with sin-gle phase HEAs.A pillar compression test under a scanning electron microscope(SEM)was performed on the individual face centered cubic(FCC),body centered cubic(BCC),and mixed-phases with different di-ameters in a Fe_(24)Co_(25)ni_(24)Cr_(23)Al_(4)HEA using focused ion beam(FIB)milling and a nanoindenter equipped with a flat *** stress-strain response of pillar underneath the indenter was selected to explore the diameter/phase-dependent size effect,the periodically fluctuation of local stress,and strain *** was revealed that the pillars at the interphase exhibited significantly higher strength,compared with the FCC and BCC *** experiment also verified the coincident mechanical size effects independent with the type of *** stress responses in the mixed-phase pillars manifested as a distinct transition from the dramatic drop to the minor fluctuation during the post-yield stages with the increasing strain,indicating the propagation of Al-ni enriched solid solution phase(BCC1)under *** the BCC1 phase,numerous dislocations were observed in the post-deformed pillars,particularly serving as the major source to enhance the strain hardening of BCC pillars.

关键词： Size effect Stress fluctuation Compression Interphase High-entropy alloys

SRAP体系优化及在胡枝子亲缘关系中的应用

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华南农业大学学报 2011年第3期32卷 63-67页

作者：骈瑞琪倪慧群欧阳昆唏李伟陈晓阳华南农业大学林学院广东省森林植物种质创新与利用重点实验室广东广州510642 北京林业大学生物科学与技术学院北京100083 华南农业大学科技处广东广州510642

利用正交设计L16(45)对胡枝子SRAP-PCR反应体系的5种因素(模板、Mg2+、dNTPs、引物、Taq酶)在4个水平上进行优化试验,筛选出各因素的最佳水平,建立了胡枝子SRAP-PCR反应的最佳体系(25μL):模板DNA40 ng,dNTPs 0.16 mmol/L,Mg2+1.5 mmol/... 详细信息

利用正交设计L16(45)对胡枝子SRAP-PCR反应体系的5种因素(模板、Mg2+、dNTPs、引物、Taq酶)在4个水平上进行优化试验,筛选出各因素的最佳水平,建立了胡枝子SRAP-PCR反应的最佳体系(25μL):模板DNA40 ng,dNTPs 0.16 mmol/L,Mg2+1.5 mmol/L,Taq酶1.0 U,引物0.64μmol/L.采用优化的扩增体系对10种胡枝子进行了SRAP分子标记分析.筛选出的10对引物共扩增出274条带,其中244条为多态性带,占89.01%.平均每对24条.多态性条带最多的是引物组合me21/Em1和me6/Em9,均达到29条.10种胡枝子种间遗传距离的变异幅度为0.221～0.632.聚类分析表明以0.49为阀值,可以将10种胡枝子分为4大类.

关键词：胡枝子 SRAP 优化亲缘关系

溶剂对聚(对亚苯基亚乙炔基-alt-间亚苯基亚乙炔基)螺旋缠绕单壁碳纳米管的影响

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高分子学报 2022年第6期53卷 683-690页

作者：黄艳琴盛况倪慧琳刘坤林张瑞范曲立黄维南京邮电大学有机电子与信息显示国家重点实验室信息材料与纳米技术研究院南京210023 西北工业大学柔性电子研究院西安710072 东南大学附属中大医院眼科南京210009

水溶性聚(对亚苯基亚乙炔基-alt-间亚苯基亚乙炔基)(meta-PPEs)可与单壁碳纳米管(SWCNTs)组装成螺旋缠绕复合结构,从而改善SWCNTs的溶解性并实现选择性分离.为深入理解这种明确形态超分子结构的形成机理,本文利用阳离子型水溶性meta-PPE... 详细信息

水溶性聚(对亚苯基亚乙炔基-alt-间亚苯基亚乙炔基)(meta-PPEs)可与单壁碳纳米管(SWCNTs)组装成螺旋缠绕复合结构,从而改善SWCNTs的溶解性并实现选择性分离.为深入理解这种明确形态超分子结构的形成机理,本文利用阳离子型水溶性meta-PPE-N^(+)Et_(2)me与SWCNTs在不同比例二甲基亚砜/水(DMSO/H_(2)O)的混合溶剂中制备复合材料,深入探索聚合物结构、溶剂成分对复合结构形态和稳定性的影响.通过紫外-可见-近红外吸收光谱、荧光发射光谱、透射电镜、拉曼光谱和红外光谱等研究表明,当DMSO比例为70%时,该聚合物呈现从无规线团到螺旋构象的突变;此时自组装形成螺旋构象的驱动力较大,因此聚合物在自身开始形成螺旋疏水空腔的过程中对SWCNTs产生螺旋缠绕,疏水效应和π-π作用进一步增强,形成稳定的复合材料;然而在其他比例混合溶剂中并没有形成明显的螺旋缠绕超分子结构.该研究结果将为SWCNTs的可控修饰和应用提升提供一定的理论指导.

关键词：单壁碳纳米管水溶性聚(亚苯基亚乙炔基) 复合材料螺旋缠绕

NAA对葡萄果实中糖酸含量及相关基因表达的影响

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园艺学报 2015年第3期42卷 425-434页

作者：王西成吴伟民赵密珍钱亚明王壮伟江苏省农业科学院园艺研究所江苏省高效园艺作物遗传改良重点实验室南京210014

以‘里扎马特’葡萄为试材,于果实始熟期分别利用50、100和200 mg·L^(-1)的NAA喷施果实。结果表明:NAA处理可显著抑制果实中葡萄糖、果糖、蔗糖以及可溶性总糖的积累,阻碍酒石酸、苹果酸、柠檬酸、草酸及总酸的降解,且以200 mg·L^(-1... 详细信息

以‘里扎马特’葡萄为试材,于果实始熟期分别利用50、100和200 mg·L^(-1)的NAA喷施果实。结果表明:NAA处理可显著抑制果实中葡萄糖、果糖、蔗糖以及可溶性总糖的积累,阻碍酒石酸、苹果酸、柠檬酸、草酸及总酸的降解,且以200 mg·L^(-1)处理最为显著,但处理并未改变果实中的糖酸组分。NAA处理还可显著抑制可溶性糖代谢关键基因SS和SPS的表达,但对ni表现为前、中期抑制,后期促进,对FRK表现为前、中期促进,后期抑制。对于有机酸代谢相关基因而言,NAA处理显著促进MDH和PEPC的表达,抑制me基因的表达,但对IDH基因表达的影响并不显著。由此推断,外源NAA处理抑制果实成熟的原因可能在于其改变了部分糖酸代谢相关基因的表达,进而阻碍了可溶性糖的积累和有机酸酸的降解。

关键词：葡萄萘乙酸可溶性糖有机酸基因表达

头影测量评估福建地区中青年咬合垂直距离的探索性研究

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实用口腔医学杂志 2021年第5期37卷 646-649页

作者：倪涧钊许亮赖丹琳魏斌黄晓红福建医科大学附属第一医院福州350005

目的:研究福建地区中青年头影测量中5种垂直距离测量指标的精确性。方法:纳入福建地区142例中青年,拍摄头影侧位片并使用Sketchpad软件测量对各硬组织指标进行分析。对测量结果进行相关性分析以及多重线性回归分析。研究以(Ar-Go-me,Gon... 详细信息

目的:研究福建地区中青年头影测量中5种垂直距离测量指标的精确性。方法:纳入福建地区142例中青年,拍摄头影侧位片并使用Sketchpad软件测量对各硬组织指标进行分析。对测量结果进行相关性分析以及多重线性回归分析。研究以(Ar-Go-me,Gonial angle)、SNA、ANS-me、Go-me、ANS-me/N-me、S-N、(N-CF-ANS,CA)为基准指标,对5个垂直距离指标(ANS-Dc-me,VDO sup)、(ANS-Go-me,VDO inf)、(ANS-xi.Pm,VDO med)、(ANS-GOC-me,LA)、FMA,进行相关性分析以及多重线性回归分析。结果:相关性分析显示五项指标与Gonial angle、SNA、ANS-me、Go-me相关并有统计学意义。并且与ANS-me/N-me大部分相关(除FMA外)。CA与S-N于5项指标相关程度相对较少。多重线性回归模型拟合程度最好的是LA(调整R^(2)=0.902),其次是VDO inf(调整R^(2)=0.900)。结论:福建地区中青年可以使用LA以及VDO inf作为咬合垂直距离确定的参考指标。

关键词：咬合垂直距离头影测量咬合重建

多重富集定量PCR(me-qPCR)同时检测4种食源性病原弧菌

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中国农业科学 2016年第23期49卷 4619-4627页

作者：魏霜汪天杰龙阳周广彪林春贵黄帅吴希阳汕头出入境检验检疫局暨南大学理工学院食品科学与工程系广州出入境检验检疫局湛江出入境检验检疫局

【目的】溶藻弧菌、副溶血弧菌、创伤弧菌、霍乱弧菌是4种重要的食源性病原弧菌,能够造成人类多种疾病,建立同时检测这4种食源性病原弧菌的检测方法是保障食品安全的基础。本研究旨在建立同时检测溶藻弧菌、副溶血弧菌、创伤弧菌、霍乱... 详细信息

【目的】溶藻弧菌、副溶血弧菌、创伤弧菌、霍乱弧菌是4种重要的食源性病原弧菌,能够造成人类多种疾病,建立同时检测这4种食源性病原弧菌的检测方法是保障食品安全的基础。本研究旨在建立同时检测溶藻弧菌、副溶血弧菌、创伤弧菌、霍乱弧菌的多重富集定量PCR(multiplex enrichment quantitative PCR,me-q PCR)方法,并含扩增内标用于指示PCR反应的假阴性,创新一种高通量、高灵敏度、具备定量能力的检测方法,为这4种食源性病原弧菌的检测提供新方法。【方法】以细菌16S r RNA为扩增内标靶序列设计引物,并针对副溶血弧菌的collagenase、溶藻弧菌的gyr B、霍乱弧菌的omp W、创伤弧菌的vvh A,分别设计内、外2对特异性引物,首先将所有内、外引物混合,进行一个循环数较少(10—20 cycles)的高通量多重富集PCR将靶基因富集出来,由于循环数较少,各个基因得到均匀的扩增,每个基因均有4种可能的产物,每1种产物均能作为第二轮巢氏荧光定量PCR的模板,这增加了靶基因从模板中被富集出来的概率,然后将产物稀释后作为模板,利用内引物分别进行巢式荧光定量PCR检测各个基因,最后根据扩增曲线和熔融曲线分析结果。通过设置不同的第一轮多重富集PCR循环数(10、15和20个循环),优化me-q PCR第一轮循环数。采用14株标准菌株的基因组DNA作为模板,评价me-q PCR的特异性。并以4种弧菌基因组DNA混合物梯度稀释的样品(100、10、1、0.1、0.01和0.001 ng·μL-1)作为模板,对建立的me-q PCR进行灵敏度和定量能力的评价。并将该方法应用于已分离到的69株疑似弧菌菌落的鉴定中,与传统生理生化鉴定结果进行对比。【结果】优化后,me-q PCR的第一轮循环数确定为15,特异性评价结果显示该方法特异性强,灵敏度达0.001 ng,高于普通荧光定量PCR约1个数量级,并能有效指示PCR反应的假阴性,且拥有与普通荧光定量PCR相同的定量能力,扩增效率和R2符合定量的要求;将建立的me-q PCR方法应用于69株疑似弧菌菌株的鉴定,24个绿色菌落为副溶血弧菌,22个黄色菌落为溶藻弧菌,1个黄色菌落为创伤弧菌,没有检出霍乱弧菌,其结果与生理生化鉴定结果一致。【结论】该方法能够定量、快速准确地检测副溶血弧菌、溶藻弧菌、霍乱弧菌和创伤弧菌这4种食源性病原弧菌,灵敏度高,并能有效指示PCR反应的假阴性,结果无需凝胶电泳,适用于食品中4种常见病原弧菌的快速筛检。

关键词：霍乱弧菌创伤弧菌副溶血弧菌溶藻弧菌扩增内标多重富集定量PCR