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海洋食品酶解物改善精神疲劳功能性评价的综合实验设计

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实验技术与管理 2024年第8期41卷 36-42页

作者：张恬恬马磊邹宏宇王成成薛长湖王玉明中国海洋大学食品科学与工程学院山东青岛266404

该文设计了以小鼠模型评价海洋食品酶解物对改善精神疲劳营养功能的创新性综合实验,以海参和牡蛎两种海洋生物资源为原料,通过酶解法制备了海参酶解物和牡蛎酶解物,测定了两种酶解物的氨基酸组成,探究了两种酶解物对皮质酮所致精神疲劳... 详细信息

该文设计了以小鼠模型评价海洋食品酶解物对改善精神疲劳营养功能的创新性综合实验,以海参和牡蛎两种海洋生物资源为原料,通过酶解法制备了海参酶解物和牡蛎酶解物,测定了两种酶解物的氨基酸组成,探究了两种酶解物对皮质酮所致精神疲劳模型小鼠耐力、焦虑样行为、抑郁样行为以及神经递质水平的影响。结果表明海参酶解物和牡蛎酶解物均可显著提高精神疲劳小鼠的耐力表现,并改善焦虑样行为和抑郁情绪,其中,海参酶解物在改善焦虑样行为方面更显著,而牡蛎酶解物在改善抑郁情绪方面更显著。进一步研究发现两种酶解物通过调节神经递质水平改善精神疲劳。该综合实验结合了海洋生物资源精深加工技术、食品检验与分析、食品营养学、功能性食品等多领域实验技术,有助于提高学生的学习积极性、深化学生所学理论知识、培养其创新思维和实验技能。

关键词：海洋食品精神疲劳高值化利用综合实验

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An efficient method for chitin production from crab shells by a natural deep eutectic solvent

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Marine Life Science & Technology 2022年第3期4卷 384-388页

作者： Wen‑Can Huang Dandan Zhao changhu xue Xiangzhao Mao College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts of Qingdao Pilot National Laboratory for Marine Science and Technology(Qingdao)Qingdao 266200China

Crab shells are an important feedstock for chitin ***,their highly compact structure significantly limits their use for the production of chitin under mild ***,a green and efficient approach using a natural deep eutectic solvent(NADES)to produce chitin from crab shells was *** effectiveness in isolating chitin was *** results showed that most proteins and minerals were removed from crab shells and the relative crystallinity of the isolated chitin reached 76%.The quality of the obtained chitin was comparable to chitin isolated by the acid–alkali *** is the first report on a green method for efficient chitin production from crab *** study is expected to open new avenues for green and efficient production of chitin from crab shells.

关键词： Chitin Food wastes Natural deep eutectic solvents Crab shells Polysaccharides

Physiological Responses of Clam(Ruditapes philippinarum)to Transport Modes with Different Temperatures

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Journal of Ocean University of China 2023年第2期22卷 517-526页

作者： BI Shijie xue changhu XU Lili WEN Yunqi WANG Lihao LI Zhaojie LIU Hongying College of Food Science and Technology Hebei Agricultural UniversityBaoding 071000China College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Ocean College of Hebei Agricultural University Qinhuangdao 066000China

Given the increased circulation time after fishing,a series of changes take place in live clams,leading to a deterioration in quality even after ***,in this study,we aimed to explore the optimal mode of transportation of *** container for holding clams was reformed,and a water circulation temperature control system was *** physiological responses of clams during anhydrous and watery transportation at two temperatures(4 and 15℃)were investigated based on the aforementioned *** comparing the transportation patterns after 3 d of transport,a higher survival rate was observed at 4℃(97%)than at 15℃(63%)in the anhydrous transportation groups and a lower survival rate was observed at 4℃(93%)than at 15℃(99%)in the watery transportation *** addition,the glycogen content,condition index(CI),and adenylate energy charge(A.E.C)value were higher at4℃((40.87±0.99)mg g^(-1),13.71%±0.50%and 57.45%±1.60%)than at 15℃((30.54±0.81)mg g^(-1),9.09%±0.30%and 43.12%±1.65%)in the anhydrous transportation *** the watery transportation groups,a lower glycogen content,CI,and *** were observed at 4℃((33.78±0.84)mg g^(-1),9.78%±0.50%and 64.65%±1.25%)than at 15℃((41.53±0.93)mg g^(-1),12.72%±0.83%and 71.58%±1.27%).Results from this study show that anhydrous transportation(4℃)is the optimal transport condition for clams to maintain a high quality and good physiological ***,this study will be particularly useful for establishing shellfish transportation systems.

关键词： watery transportation anhydrous transportation clam physiological response temperature

Dynamic changes of peptidome and release of polysaccharide in sea cucumber(Apostichopus japonicus) hydrolysates depending on enzymatic hydrolysis approaches

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Food Science and Human Wellness 2022年第5期11卷 1331-1341页

作者： Yanchao Wang Yan Song Yaoguang Chang Yanyan Liu Guangning Chen changhu xue College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266237China

Enzymatic hydrolysis has been widely used to produce bioactive hydrolysates from sea cucumber body ***,inspired by the clarification of Apostichopus japonicus genome,we investigated the enzymatic hydrolysis of sea cucumber body wall by using the omics *** proteins,including major yolk proteins,collagens,extracellular matrix glycoproteins and muscle proteins,were released from the body wall by different hydrolysis condition.A portfolio of 216 shared peptides were detected in the peptidome by papain with different hydrolysis time,while 32 shared peptides were detected in the peptidome by differing *** peptides and the relative abundance distribution profiles of shared peptides changed depending on hydrolysis approaches,indicating dynamic changes of peptidome during ***,release of sulfated fucan and fucosylated chondroitin sulfate changed with the hydrolysis *** monitoring of dynamic enzymatic hydrolysis process at a molecular scale would contribute to production and quality control of sea cucumber hydrolysates.

关键词： Sea cucumber Enzymatic hydrolysis Protein Peptidome Polysaccharide

Echinoside A from Pearsonothuria graeffei Exert the Cytotoxicity to MDA-MB-231 Cells via Mitochondrial Membrane and Modulation of PI3K/Akt/mTOR Pathway

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Journal of Ocean University of China 2023年第1期22卷 205-212页

作者： LI Hongyan CUI Huanhuan CONG Peixu XU Jie XIE Wancui WANG Yuming xue changhu College of Marine Science and Biological Engineering Qingdao University of Science and TechnologyQingdao 266042China College of Food Science and Engineering Ocean University of ChinaQingdao 266003China

A kind of triterpene glycosides echinoside A(EA)was extracted from sea cucumber Pearsonothuria graeffei,and its yield was about 0.78%.The purity of EA was 99.0%,and its molecular weight was 1206 *** was a linear tetrasaccharide attached to a pentacyclic triterpene *** inhibited the growth of MDA-MB-231 cells in *** antitumor effect was related to elevate ROS level,decrease mitochondrial membrane potential,enhance caspase-3 expression,induce cells apoptosis and arrest cell cycle at G2/M *** also dose-dependently suppressed the expressions of phophorylation proteins p-PI3K,p-Akt,and p-mTOR as analyzed by western *** results suggested that EA caused MDA-MB-231 cells apoptosis via intrinsic mitochondrial and PI3K/Akt/mTOR *** can be a potential anti-breast cancer agent to enhance the clinical efficacy.

关键词： Pearsonothuria graeffei echinoside A cytotoxicity PI3K/Akt/mTOR pathway

Docosahexaenoic acid-rich fish oil prevented insulin resistance by modulating gut microbiome and promoting colonic peptide YY expression in diet-induced obesity mice

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Food Science and Human Wellness 2022年第1期11卷 177-188页

作者： Wanxiu Cao Fang Liu Robert WLi Yaoxian Chin Yuming Wang changhu xue Qingjuan Tang College of Food Science and Engineering Ocean University of ChinaQingdao 266003China Key Laboratory of Marine Drugs Ministry of EducationSchool of Medicine and PharmacyOcean University of ChinaQingdao 266003China United States Department of Agriculture Agriculture Research Service(USDA-ARS)Animal Genomics and Improvement LaboratoryBeltsvilleMD 20705USA Hainan Tropical Ocean University Sanya 572022China Laboratory for Marine Drugs and Bioproducts Qingdao National Laboratory for Marine Science and TechnologyQingdao 266003China

It is unclear how docosahexaenoic acid(DHA)improves insulin resistance via modulating gut microbiome in obese *** used diet-induced obesity(DIO)mice as a model to study the effects of DHA-rich fish oil(DHA-FO)on host metabolic disorders and colonic ***-FO reduced fat deposition,regulated lipid profiles and alleviated insulin resistance in DIO *** because DHA-FO prevented the permeation of lipopolysaccharide across intestinal epithelial barrier,and promoted peptide YY(PYY)secretion via the mediation of short chain fatty acids receptor(FFAR2)in ***,DHA-FO might regulate PYY expression by reversing microbial dysbiosis,including increasing the abundance ofAkkermansia muciniphila and Lactobacillus,and suppressing the growth of ***-FO also altered gut microbial function(e.g."linoleic acid metabolism")associated with PYY expression(r>0.80,P<0.05).Herein,DHA-FO enhanced insulin action on glucose metabolism by altering gut microbiome and facilitating colonic PYY expression in DIO mice.

关键词： Docosahexaenoic acid Diet-induced obesity Insulin resistance Peptide YY Gut microbiome

脑卒中患者吞咽障碍恢复不良影响因素的Meta分析

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神经疾病与精神卫生 2024年第3期24卷 190-198页

作者：毛怡君薛常虎范惠李尔清张维杨薇陕西省咸阳市中心医院神经内科 712000 陕西省咸阳市中心医院护理部 712000 陕西省咸阳市中心医院介入导管室 712000

目的系统分析脑卒中患者吞咽障碍恢复不良的影响因素。方法系统检索中国知网、万方数据库、维普网、中国生物医学文献数据库、PubMed、Cochrane Library、Embase数据库,收集建库至2022年12月发表的脑卒中患者吞咽障碍恢复不良影响因素... 详细信息

目的系统分析脑卒中患者吞咽障碍恢复不良的影响因素。方法系统检索中国知网、万方数据库、维普网、中国生物医学文献数据库、PubMed、Cochrane Library、Embase数据库,收集建库至2022年12月发表的脑卒中患者吞咽障碍恢复不良影响因素相关研究,使用RevMan 5.4.1软件对纳入文献进行Meta分析。结果共纳入22篇文献,4248例患者。Meta分析结果显示,年龄≥70岁(OR=1.53,95%CI:1.29~1.80)、美国国立卫生研究院卒中量表(NIHSS)评分≥8分(OR=2.36,95%CI:1.53~3.63)、改良Rankin量表(mRS)评分>0分(OR=1.71,95%CI:1.40~2.09)、双侧卒中(OR=4.85,95%CI:2.62~8.97)、低密度脂蛋白(LDL)水平≥3 mg/L(OR=3.70,95%CI:2.46~5.56)、认知障碍(OR=6.23,95%CI:0.98~39.58)、误吸(OR=4.47,95%CI:3.28~6.11)、气管插管(OR=2.70,95%CI:1.58~4.63)是脑卒中患者吞咽功能恢复不良的危险因素(P18.5 kg/m2(OR=0.76,95%CI:0.67~0.86)、Barthel指数(BI)>60分(OR=0.37,95%CI:0.22~0.62)、功能独立性测评(FIM)评分>20分(OR=0.96,95%CI:0.94~0.99)是脑卒中患者吞咽功能恢复不良的保护因素(P<0.05)。结论高龄、高NIHSS评分、双侧卒中、低体重指数、高mRS评分、低BI、低FIM评分、高LDL水平、合并认知障碍、误吸、气管插管是脑卒中患者吞咽障碍恢复不良的危险因素,临床可结合影响因素完善吞咽障碍患者的管理措施。

关键词：脑卒中吞咽障碍恢复影响因素 Meta分析

分布式无线组网超宽带穿墙雷达系统设计与联合定位

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雷达学报（中英文） 2024年第4期13卷 747-760页

作者：刘新朱海滨刘宗强薛长虎穆雅鑫渠晓东叶盛波夏正欢方广有北京卫星信息工程研究所北京100095 北京信息科技大学机构北京100101 北京理工大学北京100081 中国科学院机构空天信息创新研究院北京100190

单发单收穿墙雷达具备便携、系统简单、可独立工作等优势,但难以实现目标二维定位与跟踪。该文基于便携式单发单收雷达设计了一套分布式无线组网穿墙雷达系统,同时提出了一种目标联合定位方法,能够兼顾系统便携、低成本和目标二维信息... 详细信息

单发单收穿墙雷达具备便携、系统简单、可独立工作等优势,但难以实现目标二维定位与跟踪。该文基于便携式单发单收雷达设计了一套分布式无线组网穿墙雷达系统,同时提出了一种目标联合定位方法,能够兼顾系统便携、低成本和目标二维信息估计。首先,设计了基于互补格雷码发射波形的超宽带雷达系统,解决了同频段多台雷达同时工作互相干扰的问题;分布式无线组网超宽带穿墙雷达系统包括3个雷达节点,并通过无线模块与数据处理中心通信。其次,提出了一种基于行为认知理论和模板匹配相结合的数据同步方法,通过识别各雷达数据中的相同运动状态来解决无线组网雷达慢时同步问题,摆脱了传统同步方法对硬件的苛刻要求。最后,提出基于Levenberg-Marquardt(L-M)最优化算法的雷达位置自估计和目标位置求解方法,实现了无先验雷达节点位置信息下的目标快速定位与跟踪。通过仿真分析与实验验证,该文设计的无线组网雷达系统可以实现目标二维定位与实时跟踪,雷达自身位置的估计精度优于0.06 m,对运动人体目标定位精度优于0.62 m。

关键词：超宽带穿墙雷达分布式组网无线组网数据同步联合定位

Structural characteristics of phenylboronic acid-modified astaxanthin ester and its effect on DSS-induced ulcerative colitis by blocking reactive oxygen species and maintaining intestinal homeostasis

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Food Science and Human Wellness 2023年

作者： Xing Qiao Hongyan Li Qun Gao Zhigao Wang Jie Xu Lu Yang changhu xue Marine Science and Biological Engineering， Qingdao University of Science and Technology Laboratory of Marine Drugs and Biological Products， Pilot National Laboratory for Marine Science and Technology (Qingdao) College of Food Science and Engineering， Ocean University of China

A novel and reactive oxygen species (ROS) responsive astaxanthin phenylboronic acid derivative (AstaD-PBA) was constructed by grafting phenylboronic acid (PBA) onto astaxanthin succinate diester (AstaD)， and its chemical structure and physicochemical property were identified. AstaD-PBA could effectively improve the ROS quenching ability in the lipopolysaccharide-induced RAW264.7 cell inflammation model. Then， the bioactivity of AstaD-PBA was studied by four zebrafish ROS-responsive inflammatory models induced by lipopolysaccharide (LPS)， copper (Cu2+)， high-fat diet， and dextran sodium sulfate (DSS). The results suggest that AstaD-PBA might have high biosafety and the best effect on ulcerative colitis (UC) induced by dextran sodium sulfate. Furtherly， AstaD-PBA significantly alleviated and treated weight loss and colonic shrinkage， inhibited inflammatory cytokines， and maintained microbiota homeostasis to improve UC in C57BL/6J mice. Alistipes and Oscillibacter were expected to be considered UC marker flora according to the Metastats analysis and Pearson correlation Mantel test (P < 0.01) of 16S rRNA gene sequencing data. In conclusion， AstaD-PBA has been promised to be a functional compound to improve UC and maintain intestinal microbiota homeostasis.

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