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chinese Science Bulletin 1999年第17期44卷 1581-1587页

作者： xie dongxu, ding fang, wang xiuqin, liu zhihua, luo aiping and wu minnational laboratory of molecular oncology, department of cell biology, cancer institute , chinese academy of medical sciences and peking union medical college , beijing 100021, china National laboratory of molecular oncology Department of Cell Biology Cancer Institute Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

To elucidate molecular mechanisms of As2O3 induced apoptosis of cancer cells in vitro, Atlas human cDNA expression analysis has been used for the profile of the known genes expressed in the human esophageal squamous carcinoma cells before and after treated by As2O3. On treating EC8712 cells with As2O3, most of the oncogenes have been down-regulated, while some tumor suppressor genes, such as DCC, up-regulated. Cyclin H decreases,

关键词： arsenic trioxide esophageal squamous carcinoma cell apoptosis gene expression.

Chromosome analysis of esophageal squamous cell carcinoma cell line KYSE 410-4 by repetitive multicolor fluorescence in situ hybridization

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Journal of Genetics and Genomics 2008年第1期35卷 11-16页

作者： Yiling Yang a, b, Jiayou Chu a, Yupeng wu b, Manli luo b, Xin Xu b, Yaling Han b, Yan Cai b, Qimin Zhanb, Mingrong wang b, a department of Genetics, institute of medical biology, peking union medical college and chinese academy of medical sciences, Kunming 650118, china b State Key laboratory of molecular oncology, cancer institute (Hospital), peking union medical college and chinese academy of medical sciences, beijing 100021, china department of Genetics Institute of Medical Biology Peking Union Medical College and Chinese Academy of Medical Sciences Kunming 650118 China State Key laboratory of molecular oncology Cancer Institute (Hospital) Peking Union Medical College and Chinese Academy of Medical Sciences Beijing 100021 China

Chromosome aberrations are distinctive features of human malignant tumors. Analysis of chromosomal changes can illuminate the molecular mechanisms underlying the development and progression of cancer. To establish the technique of multicolor fluorescence in situ hybridization (M-FISH) for identifying chromosome aberrations in esophageal carcinoma cell line KYSE 410-4, four pools of 6-color whole-chromosome painting probes have been designed and hybridized on the same metaphase spread by four rounds of repetitive FISH. Repetitive 6-color M-FISH was successfully established and the cytogenetic abnormalities in KYSE 410-4 cells were characterized. Chromosome gains occurred at 2q, 3, 8, 17p, and X. An isochromosome 3q was visualized in the cell line, which might be one intermedi- ate mechanism leading to 3p losses and/or 3q gains. Furthermore, 16 structural arrangements were detected, including four derivative chromosomes. The rearrangement of the centromeric regions accounted for approximately 44% of all rearrangements. The results added a more complete and accurate information of the genetic alterations to the classical cytogenetic description of KYSE 410-4 and provided a detailed cytogenetic background data for appropriate use of the cell line. The established 6-color M-FISH was useful for analyzing chro- mosomes in the whole genome of human tumors.

关键词： multicolor fluorescence in situ hybridization KYSE 410-4 karyotype esophageal squamous cell carcinoma

A novel element (NIRS) par-ticipates in the regulation of interleukin 2 receptor α gene expression

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chinese Science Bulletin 2002年第8期47卷 659-663页

作者： MANG Rui, wu Ninghua & SHEN YufeiNational Key laboratory of medical molecular biology, institute of Basic medical sciences, chinese academy of medical sciences and peking union medical college, beijing 100005, china National Key laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences and Peking Union Medical College Beijing China

A novel element at -153/-143 bp in the inter-leukin 2 receptor α(IL-2Rα) gene has been coined as NRE-inverse repeat sequence (NIRS) due to its inversely repeated to the known negative regulatory element (NRE) further upstream of the gene. In order to explore the role of NIRS in the expression of IL-2Rα gene, luciferase reporter plasmids driven by 4 individually deleted IL-2Rα genes promoter regions were constructed. Transfection of the reporter plasmids into Jurkat cells and HeLa cells respectively, we found that both NIRS and NRE were critical for repressing the constitutive expression of IL-2Rα gene and were also necessary for promoter activity induced by PHA. EMSA results showed that double-stranded NRE- and NIRS-bind-ing proteins existed in both HeLa cells and Jurkat cells. However, single-stranded NIRS- and NRE-binding protein was only found in HeLa cells. Interestingly, the supershift band showed up in EMSA system with Jurkat cells (no matter whether activated or not) adding to the cell

关键词： IL-2Rα gene NRE NIRS transcriptional regulation.

一个可能与T细胞发育相关的新的C2H2型锌指蛋白基因的克隆(英文)

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生物化学与生物物理进展 2004年第7期31卷 600-605页

作者：杜占文张昕张俊武中国医学科学院中国协和医科大学基础医学研究所

一些在组织和细胞分化中起重要作用的蛋白质包含锌指结构域 .为了克隆分离和研究与造血细胞分化和发育成熟相关的蛋白基因 ,利用编码C2H2型锌指蛋白结构域中部分保守氨基酸序列设计简并引物 ,以骨髓cDNA为模板 ,进行PCR扩增 ,得到若干... 详细信息

一些在组织和细胞分化中起重要作用的蛋白质包含锌指结构域 .为了克隆分离和研究与造血细胞分化和发育成熟相关的蛋白基因 ,利用编码C2H2型锌指蛋白结构域中部分保守氨基酸序列设计简并引物 ,以骨髓cDNA为模板 ,进行PCR扩增 ,得到若干新的锌指蛋白基因EST .用其中一条为探针筛选人骨髓cDNA文库 ,获得了一个新的锌指蛋白基因全长cDNA ,GenBank收录号为AF2 4 6 12 6 ,长 3888bp ,包括一个完整阅读框 ,编码 6 86个氨基酸 ,包括 17个典型的和 2个非典型的C2H2模体 ,命名为HZF2 .RNA印迹、人多组织mRNA斑点杂交分析结果显示 ,其在T淋巴细胞发育和定居的器官组织胸腺、淋巴结中有较高表达 ,在脾脏、胎肝有中度表达 ,在B淋巴细胞发育的骨髓中表达很低 ,在外周血几个淋巴细胞系中仅有极微量的表达 ,提示HZF2可能对于T淋巴细胞发育和增殖有重要功能 .该基因也在脑组织的若干部位、胎盘及肾上腺有较高表达 ,在多种其他组织细胞有微量表达 ,说明其可能对维持这些组织细胞的生理功能也起一定作用 .将编码HZF2读框的DNA顺序克隆到pEGFP N1载体中 ,转染 3T3细胞 ,证明表达的HZF2 GFP融合蛋白定位于细胞核 ,这与根据HZF2蛋白结构推测其可能作为DNA结合蛋白行使调节基因转录的功能是一致的 .

关键词：锌指蛋白基因克隆 cDNA文库筛选表达谱 T细胞发育

Chromosomal localization of a novel retinoic acid induced gene RA28 and protein distribution of its encoded protein

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chinese Science Bulletin 2000年第20期45卷 1857-1862页

作者： liu zhihua luo aiping wang xiuqin wu Min National laboratory of molecular oncology Department of Cell BiologyCancer InstituteChinese Academy of Medical Sciences&Peking Union Medical CollegeBeijing 100021China

Gene RA28 is a retinoic acid induced novel gene isolated in our laboratory previously. All-trans retinoic acid (ATRA) was used to induce lung adenocarcinoma cell line GLC-82, and RA28 was obtained by subtractive hybridization. Green fluorescent protein (GFP) has emerged as a unique tool for examining introcellular phenomena in living cells. GFP possesses an intrinsic fluorescence at 488 nm that does not require other co-factors. In this report, an eukaryotic expression plasmid pEGFP-C1-RA28 was constructed and transfected with parental cell line GLC-82 to analyze protein expression and its distribution in living cells. Moreover, radiation hybrid (RH) technique was used to localize RA28 to the chromosome. The results show that gene RA28 is mapped to the chromosome 19q13.1 region, its encoded protein is distributed on cell membrane. All the results further demonstrate that GFP and RH techniques are accurate, fast, repetitive, and will be powerful methods for investigating the gene and protein

关键词： green fluorescent protein (GFP) radiation hybrid (RH) gene localization protein localization RA28.

A competitive RT-PCR-based approach to quantifying promoter activity of stress gene

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Progress in Natural Science:Materials International 2002年第10期 24-28页

作者： MO Zhicheng, LI Xiaoyan, LI Hongfan, CHENG Xiaokuan, wu Ninghua and SHEN Yufei(National laboratory of medical molecular biology, institute of Basic medical sciences, chinese academy of medical sciences & peking union medical college, beijing 100005, china) National laboratory of medical molecular biology Institute of Basic Medical Sciences Chinese Academy of Medical Sciences & Peking Union Medical College Beijing 100005 China

Chloramphenicol acetyltransferase (CAT) as a stable reporter has been broadly adopted to detect promoter activity of many stress genes in eukaryotic cells. A simplified, convenient and relatively accurate assay to reveal CAT activities driven by heat shock gene promoter upon cell stress is reported here. A CAT mutant control plasmid pMCAT is constructed out of pBLCAT3, with a DNA segment within the CAT gene deleted. The control plasmid pMCAT along with the wild type CAT reporter gene driven by the regulatory sequence of hsp90β gene are co-transfected into Jurkat cells. Following total cellular RNA extracted and reverse transcribed, PCR is carried out to amplify two individual fragments with the same pair of synthesized primers, which bind to the identical sequences in both pMCAT and the CAT reporter plasmids. The two PCR fragments amplified from the reporter and control RNAs are identified by their size differences in agarose gel electropboresis. The ratio of intensity of the two PCR bands in the same

关键词： chloramphenicol acetyltransferase mRNA promoter activity competitive RT-PCR.

Identification and characterization of human hematopoietic mesoderm

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Science china(Life sciences) 2024年第2期67卷 320-331页

作者： Yuqi Wen Jingjing Zhao Runqing Zhang Fan liu Xiaoyuan Chen Dan wu Mengge wang Cuicui liu Pei Su Panpan Meng Yiyue Zhang Xin Gao Lu wang Hongtao wang Jiaxi Zhou State Key laboratory of Experimental Hematology National Clinical Research Center for Blood DiseasesHaihe Laboratory of Cell EcosystemInstitute of Hematology&Blood Diseases HospitalChinese Academy of Medical Sciences&Peking Union Medical CollegeTianjin 300020China Tianjin institutes of Health Science Tianjin 301600China CAMS Center for Stem cell Medicine PUMC Department of Stem Cell and Regenerative MedicineTianjin 300020China Division of cell Developmental and Integrative BiologySchool of MedicineSouth China University of TechnologyGuangzhou 510006China

The embryonic mesoderm comprises heterogeneous cell subpopulations with distinct lineage *** is unclear whether a bias for the human hematopoietic lineage emerges at this early developmental *** this study,we integrated single-cell transcriptomic analyses of human mesoderm cells from embryonic stem cells and embryos,enabling us to identify and define the molecular features of human hematopoietic mesoderm(HM)cells biased towards hematopoietic *** discovered that BMP4 plays an essential role in HM specification and can serve as a marker for HM ***,BMP4 acts as a downstream target of HDAC1,which modulates the expression of BMP4 by deacetylating its *** of HDAC significantly enhances HM specification and promotes subsequent hematopoietic cell *** conclusion,our study identifies human HM cells and describes new mechanisms for human hematopoietic development.

关键词： hematopoietic mesoderm human pluripotent stem cells human embryos BMP4 HDAC1

Zinc finger protein HZF1 promotes the proliferation of K562 cells and inhibits its apoptosis

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Zinc finger protein HZF1 promotes the proliferation of K562 ...

Identification of differentially expressed genes in esophageal cancer through SSH in combination with high throughput reverse Northern screening

2009医学前沿论坛暨第十一届全国肿瘤药理与化疗学术会议

作者： Xiao-Bo Li,fang wang,Jun-wu Zhang (National laboratory of medical molecular biology,institute of Basic medical Science,peking union medical college & chinese academy of medical sciences, 5 Dong Dan San Tiao,beijing 100005,china.)

We have previously identified and characterized a novel zinc finger protein gene HZF1 and demonstrated its important role in erythroid and megakaryocytic differentiation of K562 cells (Leukemia,20:1109-1116,2006).We recently examined other functions of *** lentiviral vector-mediated gene transfer we obtained stable cell lines with HZF1 over-expression (K562-GFP-HZF1) and stable control cell lines that integrated GFP only(K562-GFP).The cell cycles of both cell lines were analyzed by detection of intracellular DNA and the result demonstrated that *** promoted the S/G2 phase transition of cell cycle. Furthermore,both cell lines were exposed to the same concentration of AsO and we found that the cells over-expressing HZF1(K562-GFP-HZF1) could more effectively resist apoptosis induced by AsO compared with the control cells(*** accordance with the prospect that HZF1 is analogous to some of anti-apoptosis proteins regulated by mir34c,we identified two potential sites for mir34c targeting within the 3’UTR of HZF1 mRNA *** dual-luciferase reporter experiment and Western-blot assay we further proved that mir34c could knockdown the HZF1 ***,our results showed that zinc finger protein HZF1 could promote cell proliferation by facilitating the S/G2 phase transition of cell cycle and inhibit cell apoptosis induced by AsO.

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chinese Science Bulletin 2001年第14期46卷 1195-1198页

作者： ZHOU Jin liu zhihua wang xiuqin ding fang ZHOU Chuannong wu Min National laboratory of molecular oncology Department of Cell BiologyCancer InstitutePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100021China

To understand the molecular mechanisms of carcinogenesis of esophagus and to isolate genes with different expression levels in esophageal cancer, suppression sub-tractive hybridization (SSH) was combined with PCR-based cDNA synthesis and reverse Northern on the cancer tissues and matched almost normal mucosa using 5 microgram of total RNA as starting marterial. Eight genes were found expressed differentially in esophageal cancer, in which 5 were known genes and 3 were novel ones; and 6 were down-regulated in cancer tissues, while 2 were up-regulated; 6 were of mid-high abundance and 2 were of low abundance in esophagus. The results revealed that alteration in expression level of multiple genes underlied the initiation and development of esophageal cancer. The differentially expressed genes identified in this study such as liporcotin I, cystatin A, cystatin B, cytokeratin 13 may play roles in dedifferentiation, transformation and malignant proliferation of esophageal cancer. The combination of SSH with

关键词： esophageal cancer suppression subtractive hybridization (SSH) differential expression lipocortin cystatin.

Targeting epigenetic and posttranslational modifications regulating ferroptosis for the treatment of diseases

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Signal Transduction and Targeted Therapy 2024年第1期9卷 105-149页

作者： Yumin wang Jing Hu Shuang wu Joshua S.Fleishman Yulin Li Yinshi Xu Wailong Zou Jinhua wang Yukuan Feng Jichao Chen Hongquan wang department of Respiratory and Critical Care Medicine Aerospace Center HospitalPeking University Aerospace School of Clinical MedicineBeijing 100049PR China department of Pathogen biology School of Basic Medical SciencesTianjin Medical UniversityTianjin 300060PR China department of Neurology Zhongnan Hospital of Wuhan UniversityWuhan 430000PR China department of Pharmaceutical sciences College of Pharmacy and Health SciencesSt.John’s UniversityQueensNY 11439USA department of Outpatient Aerospace Center HospitalPeking University Aerospace School of Clinical MedicineBeijing 100049PR China beijing Key laboratory of Drug Target and Screening Research Institute of Materia MedicaChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100050PR China department of Pancreatic cancer Tianjin Medical University Cancer Institute and HospitalNational Clinical Research Center for CancerTianjin’s Clinical Research Center for CancerKey Laboratory of Cancer Prevention and TherapyTianjin 300060PR China

Ferroptosis,a unique modality of cell death with mechanistic and morphological differences from other cell death modes,plays a pivotal role in regulating tumorigenesis and offers a new opportunity for modulating anticancer drug *** epigenetic modifications and posttranslational modifications(PTMs)promote anticancer drug resistance,cancer progression,and *** studies indicate that epigenetic modifications can transcriptionally and translationally determine cancer cell vulnerability to ferroptosis and that ferroptosis functions as a driver in nervous system diseases(NSDs),cardiovascular diseases(CVDs),liver diseases,lung diseases,and kidney *** this review,we first summarize the core molecular mechanisms of ***,the roles of epigenetic processes,including histone PTMs,DNA methylation,and noncoding RNA regulation and PTMs,such as phosphorylation,ubiquitination,SUMOylation,acetylation,methylation,and ADP-ribosylation,are concisely *** roles of epigenetic modifications and PTMs in ferroptosis regulation in the genesis of diseases,including cancers,NSD,CVDs,liver diseases,lung diseases,and kidney diseases,as well as the application of epigenetic and PTM modulators in the therapy of these diseases,are then discussed in *** the mechanisms of ferroptosis regulation mediated by epigenetic modifications and PTMs in cancer and other diseases will facilitate the development of promising combination therapeutic regimens containing epigenetic or PTM-targeting agents and ferroptosis inducers that can be used to overcome chemotherapeutic resistance in cancer and could be used to prevent other *** addition,these mechanisms highlight potential therapeutic approaches to overcome chemoresistance in cancer or halt the genesis of other diseases.

关键词： diseases epigenetic overcome