Identification of differentially expressed genes in esophageal cancer through SSH in combination with high throughput reverse Northern screening

作     者：ZHOU Jin LIU Zhihua WANG Xiuqin DING Fang ZHOU Chuannong WU Min 

作者机构：National Laboratory of Molecular OncologyDepartment of Cell BiologyCancer InstitutePeking Union Medical College and Chinese Academy of Medical SciencesBeijing 100021China 

出 版 物：《Chinese Science Bulletin》 (Chin. Sci. Bull.)

年 卷 期：2001年第46卷第14期

页      面：1195-1198页

核心收录：

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This work was co-supported by the State Key Basic Research Program (Grant Nos. G1998051021 and G1998051205) and the National Natural Science Foundation of China (Grant Nos. 39993420 and 39990570) 

主　　题：esophageal cancer suppression subtractive hybridization (SSH) differential expression lipocortin cystatin. 

摘      要：To understand the molecular mechanisms of carcinogenesis of esophagus and to isolate genes with different expression levels in esophageal cancer, suppression sub-tractive hybridization (SSH) was combined with PCR-based cDNA synthesis and reverse Northern on the cancer tissues and matched almost normal mucosa using 5 microgram of total RNA as starting marterial. Eight genes were found expressed differentially in esophageal cancer, in which 5 were known genes and 3 were novel ones; and 6 were down-regulated in cancer tissues, while 2 were up-regulated; 6 were of mid-high abundance and 2 were of low abundance in esophagus. The results revealed that alteration in expression level of multiple genes underlied the initiation and development of esophageal cancer. The differentially expressed genes identified in this study such as liporcotin I, cystatin A, cystatin B, cytokeratin 13 may play roles in dedifferentiation, transformation and malignant proliferation of esophageal cancer. The combination of SSH with