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A machine learning approach to map crystal orientation by optical microscopy

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npj Computational Materials 2022年第1期8卷 70-78页

作者： Mallory wittwer Matteo Seita School of Mechanical and Aerospace Engineering Nanyang Technological UniversitySingaporeSingapore School of Materials Science and Engineering Nanyang Technological UniversitySingaporeSingapore

Mapping grain orientation in crystalline solids is essential to investigate the relationships between local microstructure and crystallography and interpret materials *** of the main techniques used to perform these studies is electron backscatter diffraction(EBSD).Due to the limited measurement throughput,however,EBSD is not suitable for characterizing samples with long-range microstructure heterogeneity,nor for building large material libraries that include numerous *** present a machine learning approach for high-throughput crystal orientation mapping,which relies on the optical technique called directional reflectance *** successfully apply our method on Inconel 718 specimens produced by additive manufacturing,which exhibit complex,spatially-varying *** results demonstrate that optical orientation mapping on a metal alloy is *** our method is data-driven,it can be easily extended to different alloy systems produced using different manufacturing processes.

关键词： alloy microstructure crystal

Microfluidic Extreme PCR: <1 Minute DNA Amplification in a Thin Film Disposable

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Journal of Biomedical Science and Engineering 2017年第5期10卷 219-231页

作者： James M. Trauba Carl T. wittwer Department of Pathology University of Utah School of Medicine Salt Lake City UT USA

Point-of-care diagnostic testing using PCR requires a device that is fast, economical, and practical. Sub-minute amplification has been demonstrated using high concentrations of primers and polymerase in glass capillaries, but its platform is limited to research use. A system using heated copper blocks to clamp a microfluidic flow-through PCR card fabricated from thin film polycarbonate was modeled, fabricated, and tested. Models show that fluid flowing through a thin-film device clamped between temperature-controlled copper blocks equilibrates to a temperature change in 250 milliseconds. A 2-step, 35 cycle PCR with 1.06 second cycles specifically amplified a 69-base pair fragment from a 450-base pair synthetic DNA template of random sequence with the same performance as the glass capillary system. This system demonstrates the feasibility of <1 minute PCR in an inexpensive, disposable sample container.

关键词： PCR Microfluidics Diagnostics Point-of-Care

Inositol Pyrophosphate lnsP8 Acts as an Intracellular Phosphate Signal in Arabidopsis

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Molecular Plant 2019年第11期12卷 1463-1473页

作者： Jinsong Dong Guojie Ma Liqian Sui Mengwei Wei Viswanathan Satheesh Ruyue Zhang Shenghong Ge Jinkai Li Tong-En Zhang Christopher wittwer Henning JJessen Huiming Zhang Guo-Yong An Dai-Yin Chao Dong Liu Mingguang Lei Shanghai Center for Plant Stress Biology CAS Center for Excellence in Molecular Plant SciencesChinese Academy of SciencesShanghai 200032China National Key Laboratory of Plant Molecular Genetics CAS Centre for Excellence in Molecular Plant SciencesShanghai Institute of Plant Physiology&EcologyChinese Academic of SciencesShanghai 200032China University of Chinese Academy of Sciences Beijing 100049China lnstitute of Organic Chemistry Albert-Ludwigs UniversityFreiburgAlbertstrasse 2179104 FreiburgGermany C旧SS-Centre for Integrative Biological Signalling Studies Albert-Ludwigs UniversityFreiburgAlbertstrasse 2179104 FreiburgGermany Key Laboratory of Plant Stress Biology School of Life SciencesHenan University.Kaifeng 475004China Center for Plant Biology School of Life SciencesTsinghua UniversityBeijing 100084China

The maintenance of cellular phosphate(Pi)homeostasis is of great importance in living *** SPX domain-containing protein 1(SPX1)proteins from both Arabidopsis and rice have been proposed to act as sensors of Pi *** molecular signal indicating the cellular Pi status and regulating Pi homeostasis in plants,however,remains to be identified,as Pi itself does not bind to the SPX ***,we report the identification of the inositol pyrophosphate lnsP8 as a signaling molecule that regulates Pi homeostasis in *** gel electrophoresis profiling of InsPs revealed that lnsP8 level positively correlates with cellular Pi *** demonstrated that the homologs of diphosphoinositol pentaki-sphosphate kinase(PPIP5K),VIH1 and VIH2,function redundantly to synthesize lnsP8,and that the vih1 vih2 double mutant overaccumulates ***1 directly interacts with PHR1,the central regulator of Pi starvation responses,to inhibit its function under Pi-replete ***,this interaction is compromised in the vih1 vih2 double mutant,resulting in the constitutive induction of Pi starvation-induced genes,indicating that plant cells cannot sense cellular Pi status without ***,we showed that lnsP8 could directly bind to the SPX domain of SPX1 and is essential for the interaction between SPX1 and ***,our study suggests that lnsP8 is the intracellular Pi signaling molecule serving as the ligand of SPX1 for controlling Pi homeostasis in plants.

关键词： nositol pyrophosphates lnsP8 phosphate signal SPX domain Pi homeostasis

Coronary surgery following prior pneumonectomy —A challenging clinical scenario with excellent results

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Advances in Lung Cancer 2013年第1期2卷 19-20页

作者： Stefanie Reutter Oliver Liakopoulos Yeong-Hoon Choi Thorsten Wahlers Thorsten wittwer Department of Cardiothoracic Surgery Heart Center University Hospital of Cologne Kolner Germany

Coronary artery bypass grafting (CABG) in patients with prior pneumonectomy is challenging because of the decreased pulmonary function and the anatomical alterations of the thoracic cavity. Due to extended morbidity and mortality in this special patient cohort, only very few reports in the world literature focus on this topic. Overall, no general recommendations exist with regard to the optimal management of this continuously growing patient cohort. We present our experience with another successful case.

关键词： Coronary Surgery Previous Pneumonectomy

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二十一世纪需要的农业研究与农业技术

江苏农业科学 1981年第3期 58-64页

作者： Sylvan H.wittwer 江苏省农科院情报研究室

四、水产养殖的潜力全世界年渔获量为7000万吨,其中水产养殖只有600万吨.目前水产养殖的产量比七十年代初期翻了一番多,对水产养殖的兴趣正在增长.水产养殖产量占渔获总量的比重以中国为最高,达40%.印度为38%,印尼为22%,菲律宾为10%,日... 详细信息

四、水产养殖的潜力全世界年渔获量为7000万吨,其中水产养殖只有600万吨.目前水产养殖的产量比七十年代初期翻了一番多,对水产养殖的兴趣正在增长.水产养殖产量占渔获总量的比重以中国为最高,达40%.印度为38%,印尼为22%,菲律宾为10%,日本为6%.人们对高质量蛋白质食物的需求日益增长.水产养殖也适合于发展中国家的小规模经营.要大大提高把饵料转化为鱼肉的效率.在某些情况下,水产动物的转化率可能比畜禽更高,因为它们毋需保持较高的体温.任何未受污染的淡水、咸水都可搞水产养殖.还可利用“废”水养殖.水产养殖也不象农业生产那样要消耗掉水.目前水质是一个严重的制约因素.但是适合水产养殖的水资源是有限度的,而对水资源的

关键词：农业土地经费食物生产二十一世纪

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二十一世纪需要的农业研究与农业技术

江苏农业科学 1981年第2期 59-64页

作者： Sylvan H.wittwer 江苏省农科院科技情报研究室场长密执安州农业试验场

目次前言一、影响粮食作物生产率的基本生物学过程（一）光合作用的效率与作物增产（二）生物固

关键词：农业

血管内皮生长因子基因心肌血运重建治疗冠心病

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中华胸心血管外科杂志 2000年第5期16卷 309-311页

作者：屈正张兆光首都医科大学附属北京安贞医院心脏外科 100029

目的分析上海松江区一个强直性肌营养不良(myotonic dystrophy,DM)家系分子检测的结果,以及CTG三棱苷酸重复拷贝数与临床表现的关系.方法用长模板扩展TMPCR法检测一个DM家系、23名受检者19q13.2-3上肌张力蛋白激酶基因(MTPK)3＇端非翻... 详细信息

目的分析上海松江区一个强直性肌营养不良(myotonic dystrophy,DM)家系分子检测的结果,以及CTG三棱苷酸重复拷贝数与临床表现的关系.方法用长模板扩展TMPCR法检测一个DM家系、23名受检者19q13.2-3上肌张力蛋白激酶基因(MTPK)3＇端非翻译区上CTG重复拷贝数.结果临床8例典型的DM患者中4例CTG重复序列异常扩展,另4例CTG重复拷贝数正常.8例临床可疑的DM患者中7例CTG重复序列过度扩展,确诊为DM患者,1名无症状个体为可疑DM患者.另6名家系成员有DM风险的个体及1名???????????????????

关键词：冠心病血管内皮生长因子心肌血动重建

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展望二十一世纪的新农业(下)

世界农业 1984年第4期 6-9页

作者： Sylvan H.wittwer ,未名

科学和新技术的影响遗传工程——细胞和分子生物学以及组织培养,原子能——原子释放,空间时代——地心吸力的摆脱,计算机革命——信息传递的电子化,以上四项科学领域的重大革命,构成了二十一世纪的特征。其第一和第四项与美国的农业科... 详细信息

科学和新技术的影响遗传工程——细胞和分子生物学以及组织培养,原子能——原子释放,空间时代——地心吸力的摆脱,计算机革命——信息传递的电子化,以上四项科学领域的重大革命,构成了二十一世纪的特征。其第一和第四项与美国的农业科学研究和将来的农业生产密切相关。电子计算机信息系统对农业生产和科学研究,在各种水平上,从大学、国家办的实验室、州立农事试验站、推广站、农场组织以至农场,都引起了一场技术革命。电子计算机和集成电路板将越来越小、越便宜、越多用而且容量扩大。这

关键词：二十一世纪电子化农业科学研究遗传工程基因工程生长调节剂信息传递情报传递反硝化过程分子生物学生长激素生产力生产方式牲畜家畜离子载体产量课题作物生长

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展望二十一世纪新农业(上)

世界农业 1984年第3期 13-16页

作者： Sylvan H.wittwer ,未名

1982年,全球农业生产获得创记录的丰收。这是一次空前的丰收,无论是总产量或按人平均的产量,都破了纪录。世界粮食总产量达到16.4亿吨,超过了头年的16.2亿吨。这一年生产的增长,主要来自美国。在美国,玉米的新纪录达到84亿蒲式耳,小麦... 详细信息

1982年,全球农业生产获得创记录的丰收。这是一次空前的丰收,无论是总产量或按人平均的产量,都破了纪录。世界粮食总产量达到16.4亿吨,超过了头年的16.2亿吨。这一年生产的增长,主要来自美国。在美国,玉米的新纪录达到84亿蒲式耳,小麦的达到28亿蒲式耳,大豆的达到23亿蒲式耳。增长主要来自单位面积产量的提高。欧洲共同体和加拿大的刷新纪录,阿根廷虽未能创造新纪录,但总产量也很可观。这些情况加在

关键词：粮食总产量农产品产量美国美利坚合众国北美洲农业资源食物供应劳动力大豆