Microfluidic Extreme PCR: <1 Minute DNA Amplification in a Thin Film Disposable
Microfluidic Extreme PCR: <1 Minute DNA Amplification in a Thin Film Disposable作者机构：Department of Pathology University of Utah School of Medicine Salt Lake City UT USA
出 版 物：《Journal of Biomedical Science and Engineering》 (生物医学工程（英文）)
年 卷 期：2017年第10卷第5期
页 面：219-231页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：PCR Microfluidics Diagnostics Point-of-Care
摘 要：Point-of-care diagnostic testing using PCR requires a device that is fast, economical, and practical. Sub-minute amplification has been demonstrated using high concentrations of primers and polymerase in glass capillaries, but its platform is limited to research use. A system using heated copper blocks to clamp a microfluidic flow-through PCR card fabricated from thin film polycarbonate was modeled, fabricated, and tested. Models show that fluid flowing through a thin-film device clamped between temperature-controlled copper blocks equilibrates to a temperature change in 250 milliseconds. A 2-step, 35 cycle PCR with 1.06 second cycles specifically amplified a 69-base pair fragment from a 450-base pair synthetic DNA template of random sequence with the same performance as the glass capillary system. This system demonstrates the feasibility of 1 minute PCR in an inexpensive, disposable sample container.
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