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Voltage-controlled reverse filament growth boosts resistive switching memory

Nano Research 2018年第8期11卷 4017-4025页

作者： Attilio Belmonte Umberto Celano Zhe Chen Janaki Radhaskrishnan' Augusto Redolfi Sergiu Clima Olivier Richard Hugo Bender Gouri Sankar Kar wilfried vandervorst Ludovic Goux IMEC Kapeldreef 75 B-3001 Heverlee (Leuven) Belgium Institute of Microelectronics Peking University Beijing 100871 China Department of Physics and Astronomy KU Leuven Celestijnenlaan2OOD 3001 Leuven Belgium Attilio Belmonte and Umberto Celano contributed equally to this work.

Nonvolatile memory devices based on filamentary resistance switching （KS） are among the frontrunners to fuel future devices and sensors of the internet of things （IoT） era. The capability of many two distinctive resistive states in response to an external electrical stimulus has been demonstrated. Through years of selection, cells based on the drift of metal ions, namely conductive-bridge memory devices, have shown a wide range of applications with nanosecond switching speeds, nanometer scalability, high-density, and low power-consumption. However, for low （sub-10-~A） current operation, a critical challenge is still represented by programming variability and by the stability of the conductive filament over time. Here, by introducing the concept of reverse filament growth （RFG）, we managed to control the structural reconfiguration of the conductive filament inside a memory cell with significant enhancements of each of the aforementioned properties. A first-in-class Cu-based switching device is demonstrated, with a dedicated stack that enabled us to systematically trigger RFG, thus tuning the device＇s properties. Along with nanosecond switching speeds, we achieved an endurance of up to 106 cycles with a 102 read window, with outstanding disturb immunity and optimal stability of the filament over time. Furthermore, by tuning the filament＇s shape, an excellent control of multi-level bit operations was achieved. Thus, this device offers high flexibility in memory applications.

关键词： filamentary resistiveswitching conductive bridge randomaccess memory (CBRA)VO conductive bridge negative set reverse filament growth

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Boosting the cellular potency of embryonic stem cells by spliceosome targeting

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Signal Transduction and Targeted Therapy 2021年第9期6卷 2613-2614页

作者： wilfried A.Kues Friedrich-Loeffler-lnstitut Federal Research Institute for Animal HealthBiotechnologyStem Cell PhysiologyNeustadtGermany

In recent work published in Cell,Shen et al.1 identified spliceosome inhibition in embryonic stem(ES)cells as a key mechanism for the transition from pluri-to *** inhibition,achieved by RNA interference or the chemical inhibitor pladienolide B,may gain widespread relevance to the culture of totipotent ES cells,in vitro differentiation of extra-embryonal tissue and organoids,translation to the maintenance of pluripotent cells of other mammal species,including humans,and a better molecular understanding of cellular potency in stem cells and cancer.

关键词： some culture

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Cocktails of defined chemical compounds:sufficient to induce totipotency in embryonic stem cells

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Signal Transduction and Targeted Therapy 2022年第10期7卷 3630-3631页

作者： wilfried A.Kues Dharmendra Kumar Friedrich-Loeffler-Institut Federal Research Institute for Animal HealthBiotechnologyStem Cell Physiology31535 NeustadtGermany Animal Physiology and Reproduction Division ICAR-Central Institute for Research on BuffaloesHisarHaryana 125001India

In a recent work published in Nature,Hu et al.1 report that a cocktail of three small chemicals is sufficient to induce totipotency in murine embryonic stem(ES)*** this year,two other groups identified different mixtures of chemicals,which also seem to do this job,2,3 taken together this opens a new approach to capture totipotency in vitro,and to understand the underlying pathways and their interaction.

关键词： interaction. al. induce

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Residual dynamics of thiacloprid in medical herbs marjoram,thyme,and camomile in soil

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Journal of Environmental Sciences 2007年第2期19卷 205-209页

作者： YU Yun-long WU Jia-lun STAHLER Matthias PESTEMER wilfried Department of Plant Protection College of Agriculture and Biotechnology Zhejiang University Hangzhou 310029 China Institute for Ecotoxicology and Ecochemistry in Plant Protection Federal Biological Research Centre for Agriculture and Forestry Koenigin-Luise-Strafle 19 D-14195 Berlin Germany

Thiacloprid is a new insecticide of the chloronicotinyl family. To assess its risk after application, residual characteristics of thiacloprid in marjoram, thyme, and camomile and in soil were studied under field conditions. The active ingredient was extracted from the plant material using a mixture of acetone-water. After filtration, the extract was concentrated to the aqueous phase, diluted with water, and portioned against ethyl acetate on a matrix solid phase dispersion column. Thiacloprid was extracted from soil using a mixture of methanol-water, filtered, and *** （clean up） with dichloromethane. The residues were quantified using HPLC-MS-MS. The methods were validated by recovery experiments. Thiacloprid residues in marjoram, thyme, and camomile and in soil persisted beyond 10, 14, 14, and 21 d but no residues were detected after 14, 21, 21, and 28 d, respectively. The data obtained in this study indicated that the biexponential model is more suitable than the first-order function to describe the decline of thiacloprid in fresh marjoram, fresh thyme, and dried camomile flowers with half-life （t1/2） of 1.1, 0.7, and 1.2 d, respectively. However, both the first-order function and biexponential model were found to be applicable for dissipation of thiacloprid in soil with almost the same t1/2 values of 3.5 and 3.6 d. The results indicated that thiacloprid dissipates rapidly and does not accumulate in the tested herbs and in soil.

关键词： thiacloprid marjoram (Majorana hortensis) thyme (Thymus vilgaris) camomile (Matricaria chamomilla) residue

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Prediction of the P-leaching potential of arable soils in areas with high livestock densities

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Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2006年第7期7卷 515-520页

作者： WERNER wilfried TRIMBORN Manfred PIHL Uwe Institute for Plant Nutrition University of Bonn D-531159 Bonn Germany

Due to long-term positive P-balances many surface soils in areas with high livestock density in Germany are over-supplied with available P, creating a potential for vertical P losses by leaching. In extensive studies to characterize the endangering of ground water to P pollution by chemical soil parameters it is shown that the available P content and the P concentration of the soil solution in the deeper soil layers, as indicators of the P-leaching potential, cannot be satisfactorily predicted from the available P content of the topsoils. The P equilibrium concentration in the soil solution directly above ground water table or the pipe drainage system highly depends on the relative saturation of the P-sorption capacity in this layer. A saturation index of <20% normally corresponds with P equilibrium concentrations of <0.2 mg P/L. Phytoremediation may reduce the P leaching potential of P-enriched soils only over a very long period.

关键词： P-balance P-accumulation P-saturation index Subsoil P equilibrium concentration Phytoremediation

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Applying Methods from Differential Geometry to Devise Stable and Persistent Air Layers Attached to Objects Immersed in Water

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Journal of Bionic Engineering 2009年第4期6卷 350-356页

作者： wilfried Konrad Christian Apeltauer Jrg Frauendiener Wilhelm Barthlott Anita Roth-Nebelsick Institute for Geosciences University of Tubingen D-72016 Tubingen Germany Department of Mathematics and Statistics University of Otago Dunedin 9054 New Zealand Centre of Mathematics for Applications University of Oslo NO-0317 Oslo Norway Nees Institute for Biodiversity of Plants University of Bonn D-53115 Bonn Germany State Museum of Natural History Stuttgart Rosenstein 1 D-70191 Stuttgart Germany

We describe a few mathematical tools which allow to investigate whether air-water interfaces exist(under prescribed conditions)and are mechanically stable and temporally *** terms of physics,air-water interfaces are governed by the Young-Laplace *** they are surfaces of constant mean curvature which represent solutions of a nonlinear elliptic partial differential *** explicit solutions of this equation can be obtained only in very special cases,it is -under moderately special circumstances-possible to establish the existence of a solution without actually solving the differential *** also derive criteria for mechanical stability and temporal persistence of an air *** we calculate the lifetime of a non-persistent air ***,we apply these tools to two examples which exhibit the symmetries of 2D *** examples can be viewed as abstractions of the biological model represented by the aquatic fern Salvinia.

关键词： interfaces air layers differential geometry stability persistence Salvinia

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Detection of leaf structures in close-range hyperspectral images using morphological fusion

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Geo-Spatial Information Science 2017年第4期20卷 325-332页

作者： Gladys Villegas Wenzhi Liao Ronald Criollo wilfried Philips Daniel Ochoa Department of Telecommunications and Information Processing Ghent University-imec Ghent Belgium Facultad de Ingeniería en Eléctrica y Computación ESPOL Polytechnic University Escuela Superior Politécnica del Litoral Guayaquil Ecuador

Close-range hyperspectral images are a promising source of information in plant biology,in particular,for in vivo study of physiological *** this study,we investigate how data fusion can improve the detection of leaf elements by combining pixel reflectance and morphological *** detection of image regions associated to the leaf structures is the first step toward quantitative analysis on the physical effects that genetic manipulation,disease infections,and environmental conditions have in *** tested our fusion approach on Musa acuminata (banana) leaf images and compared its discriminant capability to similar techniques used in remote *** results demonstrate the efficiency of our fusion approach,with significant improvements over some conventional methods.

关键词： Hyperspectral fusion morphology plant biology

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Attractive but Toxic： Emerging Roles of Glycosidically Bound Volatiles and Glycosyltransferases Involved in Their Formation

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Molecular Plant 2018年第10期11卷 1225-1236页

作者： Chuankui Song Katja Hartl Kate McGraphery Thomas Hoffmann wilfried Schwab State Key Laboratory of Tea Plant Biology and Utilization Anhui Agricultural University West Changjiang Road Hefei Anhui 230036 China Biotechnology of Natural Products Technische Universitat Munchen Liesel-Beckmann-Str. 1 Freising 85354 Germany

Plants emit an overabundance of volatile compounds, which act in their producers either as appreciated attractants to lure beneficial animals or as repellent toxins to deter pests in a species-specific and concentration-dependent manner. Plants have evolved solutions to provide sufficient volatiles without poisoning themselves. Uridine-diphosphate sugar-dependent glycosyltransferases （UGTs） acting on vola-tiles is one important part of this sophisticated system, which balances the levels of bioactive metabolites and prepares them for cellular and long-distance transport and storage but enables the remobilization of disarmed toxins for the benefit of plant protection. This review provides an overview of the research history of glycosidically bound volatiles （GBVs）, a relatively new group of plant secondary metabolites, and discusses the role of UGTs in the production of GBVs for plant protection.

关键词： glycosyltransferases terpenoids volatiles plant protection transport storage

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Does disability increase households’health financial risk:evidence from the Uganda demographic and health survey

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Global Health Research and Policy 2022年第1期7卷 577-584页

作者： wilfried Guets Deepak Kumar Behera Univ Lyon UniversitéLumière Lyon 2GATE UMR 582469130 EcullyFrance Manipal Academy of Higher Education Manipal 576104India

Background:In the last few years,there has been a worldwide commitment to protect the vulnerable individuals from higher financial risk through out-of-pocket(OOP)health *** study examines the influence of disability and socio-demographic factors on households’health financial risks in ***:We used nationally representative cross-sectional data from the Uganda Demographic and Health Survey(UDHS)collected in 2016 by the Uganda Bureau of Statistics(UBOS)in *** measured financial risk(households’health expenditure)by money paid for health care *** estimated the“probit”model to investigate the effect of disability on health financial ***:A total of 19,305 households were included in this *** 32%of households paid money for health care services access,among which 32%paid through *** 41%of household heads were affected by *** majority(73%)of families went to the public sector for health care *** mean age was 45 years(SD±15).We find that disability is significantly associated with the household financial risk(p<0.01).The private sector’s choice for health care services is likely to positively affect the financial risk compared to the public sector(p<0.01).The wealthier the household was,the more money paid for health service was(p<0.01).Conclusion:Our results indicated that disability and household socio-demographic characteristics were associated with health financial risk in *** families with disability and experiencing difficult living conditions constitute an entry point for health authorities to enhance health coverage progress in low and middle-income countries.

关键词： Disability Health financial risk Healthcare Payment Socio-Demographic Household Survey Uganda

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Potential of transposon-mediated cellular reprogramming towards cell-based therapies

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World Journal of Stem Cells 2020年第7期12卷 527-544页

作者： Dharmendra Kumar Taruna Anand Thirumala R Talluri wilfried A Kues Animal Physiology and Reproduction Division ICAR-Central Institute for Research on BuffaloesHisar 125001India NCVTC ICAR-National Research Centre on EquinesHisar 125001India Equine Production Campus ICAR-National Research Centre on EquinesBikaner 334001India Friedrich-Loeffler-Institut Institute of Farm Animal GeneticsDepartment of BiotechnologyMariensee 31535Germany

Induced pluripotent stem(iPS)cells present a seminal discovery in cell biology and promise to support innovative treatments of so far incurable *** translate iPS technology into clinical trials,the safety and stability of these reprogrammed cells needs to be *** recent years,different non-viral transposon systems have been developed for the induction of cellular pluripotency,and for the directed differentiation into desired cell *** this review,we summarize the current state of the art of different transposon systems in iPS-based cell therapies.

关键词： Transposons Induced pluripotent stem cells Clinical applications Cellular reprogramming Cell-based therapy Genetic correction

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