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短小橘杆菌(Brevibacterium antarcticum)WN5代谢产物对烟苗的促生效应

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微生物学通报 2024年第8期51卷 3032-3040页

作者：李英民殷文闻柳霞刘京黄莺贵州大学烟草学院贵州贵阳550025 贵州省烟草品质研究重点实验室贵州贵阳550025 贵州省烟草公司遵义市公司贵州遵义563000

【背景】短小橘杆菌(Brevibacterium antarcticum)菌株WN5筛选自贵州植烟土壤,具有氨转化能力,但其对烟草促生应用效果研究的报道较少。【目的】研究菌株WN5代谢产物对烟苗的促生作用。【方法】在烟苗十字期施用菌株WN5培养液,通过对烟... 详细信息

【背景】短小橘杆菌(Brevibacterium antarcticum)菌株WN5筛选自贵州植烟土壤,具有氨转化能力,但其对烟草促生应用效果研究的报道较少。【目的】研究菌株WN5代谢产物对烟苗的促生作用。【方法】在烟苗十字期施用菌株WN5培养液,通过对烟苗外观农艺性状、根系形态结构及活性、氮素养分和抗逆性生理指标等数据的分析,探讨其促生效应。【结果】在菌株培养液中测定出了4种激素及激素合成前体:1-氨基环丙烷-1-羧酸、反式玉米素、吲哚-3-乙酸和水杨酸,将其施用在烟苗生长基质中,可促进地上部分生长,烟苗株高、茎围和鲜重分别增加42.74%、12.91%和5.00%,最大叶面积和叶片数也有增加趋势,但未达显著差异水平;烟苗叶绿素含量较对照增加32.86%;菌液处理根系的总面积、表面积、平均直径、平均长度、体积和根系活力分别显著增加35.42%、20.54%、18.22%、24.07%、36.56%和29.01%;对根系的促进作用大于地上部,根冠比显著提高41.67%。菌株WN5处理还提升了烟苗吸收养分的能力和抗逆能力,硝酸还原酶(nitrate reductase,NR)活性提升32.97%,过氧化氢酶(catalase,CAT)活性、超氧化物歧化酶(superoxide dismutase,SOD)活性和可溶性蛋白(soluble protein,SP)增加了36.31%、16.23%和18.50%。【结论】菌株WN5通过产生植物激素显著促进烟苗地上部与根系的生长,提高根系活力,增强对氮素的吸收能力和烟苗的抗氧化应激能力,具有广泛的应用潜力。

关键词：烟苗代谢产物促生菌促生效果

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健康教育在慢阻肺合并肺部感染治疗中的效果

健康教育与健康促进 2024年第4期19卷 419-420,433页

作者：殷雯雯夏雨威海市立第二医院威海264200

目的探究健康教育在慢阻肺并发肺部感染治疗中的应用效果。方法于2021年3月—2024年3月在某院选择110名慢阻肺合并肺部感染患者,采用随机数表将患者分为对照组和观察组,每组55例。两组均应用盐酸氨溴索治疗,对照组给予常规护理,观察组... 详细信息

目的探究健康教育在慢阻肺并发肺部感染治疗中的应用效果。方法于2021年3月—2024年3月在某院选择110名慢阻肺合并肺部感染患者,采用随机数表将患者分为对照组和观察组,每组55例。两组均应用盐酸氨溴索治疗,对照组给予常规护理,观察组在对照组基础上加以健康教育干预,比较两组患者的治疗效果。结果观察组的症状缓解时间、肺功能指标、炎症因子水平、健康知识得分和不良反应发生率均呈现明显改善(P<0.05)。结论在对慢阻肺合并肺部感染患者应用氨溴索盐酸治疗时实行健康教育可提高治疗效果、可降低不良反应的发生风险。

关键词：健康教育慢阻肺肺部感染

亚甲基四氢叶酸还原酶基因多态性与阿尔茨海默病的相关性临床研究

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安徽医科大学学报 2024年第6期59卷 1081-1088页

作者：游孟哲周霞尹文文万珂孙中武安徽医科大学第二附属医院康复医学科合肥230601 安徽医科大学第一附属医院神经内科合肥230022

目的探讨阿尔茨海默病(AD)进程中,亚甲基四氢叶酸还原酶(MTHFR)C677T多态性与疾病的相关性,以及是否受APOE基因影响。方法共纳入74例AD患者、85例遗忘型轻度认知障碍患者(aMCI)和81例健康对照者(HC),检测3组血清同型半胱氨酸(Hcy)、叶... 详细信息

目的探讨阿尔茨海默病(AD)进程中,亚甲基四氢叶酸还原酶(MTHFR)C677T多态性与疾病的相关性,以及是否受APOE基因影响。方法共纳入74例AD患者、85例遗忘型轻度认知障碍患者(aMCI)和81例健康对照者(HC),检测3组血清同型半胱氨酸(Hcy)、叶酸和维生素B12水平,以及MTHFR C677T及APOE基因型,通过Logistic回归分析比较MTHFR C677T不同等位基因和基因型分别与aMCI和AD风险之间的关联,以及在不同APOEε4亚组中的表现。结果AD组和aMCI组的血清Hcy水平均较对照组显著升高(P<0.001,P<0.001),而aMCI组血清叶酸水平较HC组明显降低(P=0.017)。与MTHFR CC、CT基因型者相比,TT基因型者的血清叶酸水平明显降低(P=0.038),血清Hcy水平明显升高(P=0.002)。回归分析结果显示:在APOEε4非携带者亚组中,MTHFR TT基因型可能增加aMCI患病风险(OR=3.670,95%CI=1.077-12.509,P=0.038),在APOEε4携带者中则无相应表现。结论MTHFR C677T多态性可导致血清Hcy水平增高和叶酸水平降低。在APOEε4非携带者中,MTHFR TT基因型可能增加aMCI的患病风险。

关键词：阿尔茨海默病遗忘型轻度认知障碍亚甲基四氢叶酸还原酶同型半胱氨酸

Effects of electroacupuncture versus nimodipine on long-term potentiation and synaptophysin expression in a rat model of vascular dementia

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Neural Regeneration Research 2011年第30期6卷 2357-2361页

作者： Dengming Wei Xuemin Jia Xiangxu yin wenwen Jiang Department of Pathology Medical School of Ningbo University Ningbo 315211 Zhejiang Province China

The present study stimulated Baihui （DU 20） and Dazhui （DU 14） acupoints in a rat model of vascular dementia with electroacupuncture to investigate changes in long-term potentiation and synaptophysin expression in the hippocampus. The results revealed that synaptophysin expression in brain tissues was increased after electroacupuncture. After high4requency stimulation, the population spike latency was shortened and the excitatory postsynaptic potential slope and population spike amplitude were increased. In addition, cognitive function was enhanced, similar to the effects of intragastric perfusion of nimodipine. The results indicated that electroacupuncture at Baihui and Dazhui acupoints can improve learning and memory functions of a rat model of vascular dementia by promoting synaptophysin expression, enhancing hippocampal synaptic plasticity and accelerating synaptic transmission.

关键词： vascular dementia electroacupuncture learning and memory ability long-term potentiation synaptophysin neural regeneration

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板栗新式复合饮品的消费者心理与行为研究

中国果菜 2024年第10期44卷 76-80,84页

作者：张文鞠佳桐尹雯雯吴莹瑶张美武汉工程大学环境生态与生物工程学院湖北武汉430205 武汉工程大学计算机科学与工程学院湖北武汉430205 武汉工程大学光电信息与能源工程学院数理学院湖北武汉430205 武汉工程大学法商学院湖北武汉430205 绿色化工过程教育部重点实验室(武汉工程大学) 湖北武汉430205 磷资源开发利用教育部工程研究中心湖北武汉430205 新型反应器与绿色化学工艺湖北省重点实验室湖北武汉430205

板栗是我国七大优势经济林树种之一,但我国板栗产业精深加工不足,与板栗相关的商品较为单一,缺乏多样性。为开发出消费者喜爱的板栗饮品,本研究对多个省区的消费者进行了问卷调查,收集主流社交平台上的用户评价,采用交叉分析、关联分析... 详细信息

板栗是我国七大优势经济林树种之一,但我国板栗产业精深加工不足,与板栗相关的商品较为单一,缺乏多样性。为开发出消费者喜爱的板栗饮品,本研究对多个省区的消费者进行了问卷调查,收集主流社交平台上的用户评价,采用交叉分析、关联分析、文本挖掘等方法,分析了消费者的消费倾向,生成消费者的用户画像,并针对不同年龄段提出相应的推广建议。结果显示,板栗牛奶与板栗绿茶是非常符合消费者倾向的板栗复合饮品,具有较好的市场接受度与市场前景,是目前开发潜力较大的两款产品。同时,各年龄段的消费群体的口味需求不同。18~25岁的年轻消费者偏向清爽香甜型产品,25~35岁的青年消费者偏向微苦提神型产品,35~45岁的壮年消费者偏向浓郁醇厚型产品,45岁以上的中老年及老年消费者偏向清香浓郁型产品。本研究为设计出更符合消费者偏好的板栗新式复合饮品提供了参考依据,以解决板栗贮藏困难的问题,提升板栗产品的附加值。

关键词：板栗复合饮品文本挖掘用户画像数据分析

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Novel benzamido derivatives as PTP1B inhibitors with anti-hyperglycemic and lipid-lowering efficacy

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Acta Pharmaceutica Sinica B 2018年第6期8卷 919-932页

作者： Yanbo Tang Xiaolin Zhang Zheng Chen wenwen yin Guanglei Nan Jinying Tian Fei Ye Zhiyan Xiao Beijing Key Laboratory of Active Substance Discovery and Druggability Evaluation Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College Beijing Key Laboratory of New Drug Mechanisms and Pharmacological Evaluation Study Institute of Materia Medica Chinese Academy of Medical Sciences and Peking Union Medical College

Based on a non-competitive and selective PTP1 B inhibitor reported by us previously, thirtynine benzamido derivatives were designed and synthesized as novel PTP1 B inhibitors. Among them,twelve compounds exhibited IC_(50) values at micromolar level against human recombinant PTP1 B, and most of them exhibited significant selectivity to PTP1 B over TC-PTP and CD45. Further evaluation of the most potent compound 27 on high-fat diet(HFD)-induced insulin-resistant(IR) obese mice indicated that27 could modulate glucose metabolism and ameliorate dyslipidemia simultaneously.& 2018 Chinese Pharmaceutical Association and Institute of Materia Medica, Chinese Academy of Medical Sciences. Production and hosting by Elsevier B.V. This is an open access article under the CC BY-NC-ND license(http://***/licenses/by-nc-nd/4.0/).

关键词： PTP1B inhibitors Benzamido derivatives Structureactivity relationship Anti-hyperglycemic Lipid-lowering

Elaborate Design of Two Novel Fluorescent Zinc-based Metal-Organic Frameworks for Highly Efficient Tetracycline Antibiotics Detection

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Chemical Research in Chinese Universities 2024年第3期40卷 499-507页

作者： TAO He JI Chengshan ZHANG Jian yin Yuanyuan JIA wenwen JIANG Xin XU Jie YANG Yulin MIT Key Laboratory of Critical Materials Technology for New Energy Conversion and Storage School of Chemistry and Chemical EngineeringHarbin Institute of TechnologyHarbin 150001P.R.China

The abuse of tetracycline antibiotics has caused great harm to human health and *** inexpensive,convenient and sensitive methods for the detection of tetracycline antibiotics is highly ***,based on the H_(4)ddp ligand[H_(4)ddp=3-(3,5-dicarboxyphenyl)pyridine-2,6-dicarboxylic acid],two novel zinc-based metal-organic frameworks(MOFs){[Zn_(3)(ddp)_(2)(H_(2)O)_(4)]·3H_(2)O}n(Zn1-ddp)and{[Zn_(3)(ddp)_(2)(H_(2)O)_(4)]·3H_(2)O}n(Zn2-ddp)were successfully designed by delicate structural *** Zn1-ddp and Zn2-ddp exhibited excellent water and chemical stability and showed excellent fluorescence quenching performance for tetracycline ***,the more advanced framework structure and better fluorescent performance make Zn1-ddp more sensitive than Zn2-ddp in fluorescent detection with a detection limit of 0.29µmol/L for tetracycline(TC),0.09µmol/L for doxycycline(DOX),0.10µmol/L for minocycline(MIN)and metacycline(MEL),0.19µmol/L for chlortetracycline(CTC),and 0.67µmol/L for oxytetracycline(OTC)among tetracycline *** fluorescence quenching mechanism of Zn1-ddp and Zn2-ddp for tetracycline antibiotics detection was deeply *** reasons for the superior detection performance of Zn1-ddp over Zn2-ddp were also analyzed in depth through Fourier transform infrared spectrophotometry(FTIR),X-ray photoelectron spectroscopy(XPS)analysis and framework structure *** developed method opens up a new perspective for antibiotics detection based on zinc-based MOFs.

关键词： Water system Metal-organic framework Fluorescent detection Tetracycline antibiotic

Interaction mechanism of cyanide with pyrite during the cyanidation of pyrite and the decyanation of pyrite cyanide residues by chemical oxidation

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International Journal of Minerals,Metallurgy and Materials 2024年第9期31卷 1996-2005页

作者： wenwen Han Hongying Yang Linlin Tong Key Laboratory for Ecological Metallurgy of Multimetallic Mineral(Ministry of Education) Northeastern UniversityShenyang 110819China School of Metallurgy Northeastern UniversityShenyang 110819China

The toxic cyanides in cyanide residues produced from cyanidation process for gold extraction are harmful to the *** is one of the main minerals existing in cyanide *** this work,the interaction of cyanide with pyrite and the decyanation of pyrite cyanide residue were *** revealed that high pH value,high cyanide concentration,and high pyrite dosage promoted the interaction of cyanide with *** cyanidation of pyrite was pseudo-second-order with respect to *** decyanation of pyrite cyanide residue by Na_(2)SO_(3)/air oxidation was *** cyanide removal efficiency was 83.9% after 1 h of reaction time under the optimal conditions of pH value of 11.2,SO_(3)^(2-) dosage of 22 mg·g^(-1),and air flow rate of 1.46 L·min^(-1).X-ray photoelectron spectroscopy analysis of the pyrite samples showed the formation of Fe(Ⅲ)and FeSO_(4) during the cyanidation *** cyanide that adsorbed on the pyrite surface after cyanidation mainly existed in the forms of free cyanide(CN^(-))and ferrocyanide(Fe(CN)_(6)^(4-)),which were effectively removed by Na_(2)SO_(3)/air *** the decyanation process,air intake promoted pyrite oxidation and weakened cyanide adsorption on the pyrite *** study has practical significance for gold enterprises aiming to mitigate the environmental impact related to cyanide residues.

关键词： pyrite cyanide decyanation sodium sulfite/air oxidation cyanide residue

An Improved UNet Lightweight Network for Semantic Segmentation of Weed Images in Corn Fields

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Computers, Materials & Continua 2024年第6期79卷 4413-4431页

作者： Yu Zuo wenwen Li School of Information and Control Engineering Jilin Institute of Chemical TechnologyJilin132022China School of Mechanical and Control Engineering Baicheng Normal UniversityBaicheng137000China

In cornfields,factors such as the similarity between corn seedlings and weeds and the blurring of plant edge details pose challenges to corn and weed *** addition,remote areas such as farmland are usually constrained by limited computational resources and limited collected ***,it becomes necessary to lighten the model to better adapt to complex cornfield scene,and make full use of the limited data *** this paper,we propose an improved image segmentation algorithm based on ***,the inverted residual structure is introduced into the contraction path to reduce the number of parameters in the training process and improve the feature extraction ability;secondly,the pyramid pooling module is introduced to enhance the network’s ability of acquiring contextual information as well as the ability of dealing with the small target loss problem;and lastly,Finally,to further enhance the segmentation capability of the model,the squeeze and excitation mechanism is introduced in the expansion *** used images of corn seedlings collected in the field and publicly available corn weed datasets to evaluate the improved *** improved model has a total parameter of 3.79 M and miou can achieve 87.9%.The fps on a single 3050 ti video card is about *** experimental results show that the network proposed in this paper can quickly segment corn weeds in a cornfield scenario with good segmentation accuracy.

关键词： Semantic segmentation deep learning UNet pyramid pooling module