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Isolation of microsatellite loci and reliable genotyping using noninvasive samples of a critically endangered primate, Trachypithecus leucocephalus

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Integrative Zoology 2016年第4期11卷 250-262页

作者： weiran wang Yu QIAO Yitao ZHENG Meng YAO School of Life Sciences Peking UniversityBeijingChina

Genetic information can be critical in identifying conservation priorities and developing conservation *** is an urgent need for noninvasive genetic tools to study the wild populations of Asian colobine *** majority of these species are threatened with habitat destruction,population reduction and even extinction,but generally lack information on their genetic diversity and population *** sampling and tissue collection have been scarce in these species owing to strict regulations on manipulation of endangered species,and the difficulties and risks associated with capturing these arboreal and fast-moving monkeys in the challenging environments that they *** difficulties have hindered the development of molecular genetic markers,which are usually derived from tissues or *** this study,we present a method for de novo microsatellite isolation and genotyping using DNA from noninvasive origins of a critically endangered Asian colobine,the white-headed langur(Trachypithecus leucocephalus).Genomic DNA isolated from hair was shown to be sufficient for microsatellite enrichment and isolation,with similar isolation efficiencies as from tissue *** identified and characterized 20 polymorphic microsatellite loci,and evaluated their amplification success and genotyping reliability with 86 field-collected fecal *** results show that this panel of loci can produce reliable genotypes from fecal samples,and represent a useful tool for noninvasive investigation of genetic structure,individual identification and kinship assessment in this highly endangered *** approach can be applied to conservation genetic studies of other wild species that lack sequence information and tissue samples.

关键词： Asian colobine conservation genetics FIASCO hair scatology

co-Condensation Synthesis of Salicylaldimine Calcium Complex Containing Mesoporous Silica Nanoparticles as Carriers for Drug Release

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Chemical Research in Chinese Universities 2014年第4期30卷 531-537页

作者： TANG Duihai ZHANG weiran wang Yifan MIAO Jing QIAO Zhen'an HUO Qisheng ZHANG Lirong State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun 130012 P. R. China

A series of functional mesoporous silica nanoparticles（MSNs） was synthesized by a one-step simple synthesis approach involving co-condensation of tetraethoxysilane（TEOS） and salicylaldimine ligand（Sal-Si） in the presence of cetyltrimethylammonium chloride（CTAC） under basic *** target MSNs with different sizes （50,100 and 200 nm,respectively） were ***,the Ca^2＋ cations were also introduced into *** prepared nanoparticles were characterized by means of infrared（IR） spectra,thermogravimetric analysis（TGA）,inductively coupled plasma（ICP）,CHN elemental analysis,nitrogen adsorption-desorption,scanning electron microscope（SEM） and transmission electron microscope（TEM）.Ibuprofen（IBU） which contains carboxyl groups was selected as a model *** results of drug loading and release reveal that the loading capacities and release behaviors of the model drug are highly dependent on the Ca^2＋ cations in *** release of IBU from the MSNs functionalized by Ca^2＋ cations is found to be effectively controlled when compared to the release from the MSNs without the functionalization of Ca^2＋ cations,which is due to the ionic interaction between carboxyl groups in IBU and Ca^2＋ cations in MSNs.

关键词： Mesoporous silica nanoparticle co-Condensation method Release of ibuprofen Calcium ion

The Prospects of the Eurasian Economic Union

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Contemporary International Relations 2015年第5期25卷 91-104,5页

作者： wang weiran wang Jingliang Tang Tianbo Research Center on China(Xinjiang) and the Central Asian Regional Economic Cooperation Xinjiang University of Finance and Economy the Business School Xinjiang Normal University the Finance and Tax School Hebei University of Economics and Business

Russia, after realizing that it will not be truly accepted as a member of the western world, has shifted towards promoting the integration of the Eurasian areas in order to lay the geopolitical foundations for its renewed ambition as a great power. In January 1995, the three countries of Russia, Belarus and Kazakhstan established a customs union. In March 1996, Kyrgyzstan officially announced that it would be joining the union, and the four-country customs union was established. It was aimed at coordinating the economic reform pace of the four member countries, as well as accelerating economic integration between them. In February 1999, Tajikistan also joined, and in 2000, the aforementioned five countries signed the treaty that transformed the customs union into the Eurasian Economic Community. On May 31, 2001, the Interstate Council of the Eurasian Economic Community held its first meeting in Minsk, the capital of Belarus, declaring the official founding of the Community.

关键词：经济改革联盟经济一体化经济共同体白俄罗斯吉尔吉斯斯坦哈萨克斯坦塔吉克斯坦

Attenuation of ethylene signaling increases cotton resistance to a defoliating strain of Verticillium dahliae

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The Crop Journal 2023年第1期11卷 89-98页

作者： Tianyi wang Muhammad Shaban Junhui Shi weiran wang Shiming Liu Xinhui Nie Yu Yu Jie Kong Steven J.Klosterman Xianlong Zhang Alifu Aierxi Longfu Zhu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Department of Plant Breeding and Genetics University of Agricu Institute of Economic Crops Xinjiang Academy of Agricultural SciencesKuqa 842000XinjiangChina Key Laboratory of Oasis Eco-agriculture of the Xinjiang Production and Construction Crops College of AgronomyShihezi UniversityShihezi 832000XinjiangChina Xinjiang Academy of Agricultural&Reclamation Sciences Shihezi 832000XinjiangChina United States Department of Agriculture Agricultural Research ServiceSalinasCA 93905USA

The severity of Verticillium wilt on cotton caused by defoliating strains of Verticillium dahliae has gradually increased and threatens production worldwide. Identification of the molecular components of leaf defoliation may increase cotton tolerance to V. dahliae. Ethylene, a major player in plant physiological processes, is often associated with senescence and defoliation of plants. We investigated the cotton–*** interaction with a focus on the role of ethylene in defoliation and defense against V. *** plants inoculated with V. dahliae isolate V991, a defoliating strain, accumulated more ethylene and showed increased disease symptoms than those inoculated with a non-defoliating strain. In cotton with a transiently silenced ethylene synthesis gene(GhACOs) and signaling gene(GhEINs) during cotton–V. dahliae interaction, ethylene produced was derived from cotton and more ethylene increased cotton susceptibility and defoliation rate. Overexpression of AtCTR1, a negative regulator in ethylene signaling, in cotton reduced sensitivity to ethylene and increased plant resistance to V. ***, the results indicated precise regulation of ethylene synthesis or signaling pathways improve cotton resistant to Verticillium wilt.

关键词： Cotton Verticillium dahilae Ethylene Defoliation

miR398b negatively regulates cotton immune responses to Verticillium dahliae via multiple targets

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The Crop Journal 2022年第4期10卷 1026-1036页

作者： Yuhuan Miao Kun Chen Jinwu Deng Lin Zhang weiran wang Jie Kong Steven J.Klosterman Xianlong Zhang Alifu Aierxi Longfu Zhu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Hubei University of Chinese Medicine Wuhan 430065HubeiChina Oil Crops Research Institute Chinese Academy of Agricultural SciencesWuhan 430062HubeiChina Institute of Economic Crops Xinjiang Academy of Agricultural SciencesUrumchi 842000XinjiangChina United States Department of Agriculture Agricultural Research ServiceSalinasCA 93905USA

MicroRNAs(miRNAs)play essential roles in plant defense responses,although such roles have not been identified in cotton in response to the plant pathogenic fungus Verticillium *** this study,the functions of miR398b and its target genes in *** interaction were *** transcript levels of miR398b were down-regulated by *** infection and miR398b overexpression in cotton made the plants more susceptible to *** results suggest that miR398b negatively regulates cotton resistance to *** may occur by miR398b repression of some CC-NBS-LRR genes via translational inhibition,interfering with defense responses and leading to cotton susceptibility to ***,miR398b may guide the cleavage of the mRNAs of GhCSD1,GhCSD2 and GhCCS,each of which functions in reactive oxygen species(ROS)regulation and homeostasis,thereby causing excessive ROS accumulation in miR398b-overexpressing plants in response to *** *** study suggests conserved and novel roles of miR398b in the cotton–*** *** discoveries may be coupled with new strategies in cotton breeding programs to improve resistance to ***.

关键词： Cotton miR398b NBS-LRR ROS homeostasis Verticillium dahliae

The Microscopic Surgical Treatment for Tumor of Posterior Cranial Fossa in Children

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Chinese Journal of Clinical Oncology 2009年第2期6卷 95-99页

作者： Duo Chen Xiangtai Wei Qiang Yin Junhong Guan weiran Pan Chenglin wang Yunhui Liu Department of Neurosurgery Shengjing Hospital of China Medical University Shenyang110004 Liaoning Province China

OBJECTIVE To analyze and discuss about the clinicalcharacteristics, pathological types, surgical modalities andtechniques, and postoperative complications in children withtumor of posterior cranial fossa .METHODS Retrospective study was conducted on 102 cases ofpediatric tumor of posterior cranial fossa, admitted and treated inour hospital during the period of January 1996 to January *** patients underwent microscopic surgical treatment. Fifty-eight were male and 44 cases were female. The age ranged from 9months to 14 years old, with an average of 6.1 ± 0.5 of age. CranialCT or MRI examination was conducted before and after thesurgery on all *** The primary manifestations for this group of patientswere increased intracranial pressure and/or ataxia. Postoperativepathological diagnoses showed: 46 cases of medulloblastoma, 43cases of astrocytoma, 11 cases of ependymoma (including 1 caseof degenerative ependymoma), 1 case of dermoid cyst, and 1 caseof teratoma. In this group of the patients, radical surgery wasused in 68 cases and subtotal surgical removal used in 31 cases,while surgical removal of large section was performed on 3 *** were no deaths from surgery reported. Ninety-one casesshowed significant symptomatic improvement when comparedwith preoperative conditions, while 11 cases showed either noimprovement or more severely affected afterward. For 6 cases,postoperative ventriculoperitoneal shunt was performed within7 days to 2 months after the surgery. Sixty-three patients gainedfollow-up for 3 to 60 months in duration. Thirty-nine patientsregained normal life and were able to learn well, while there were7 patients who could not live normally on their own. During thefollow-up period, there were 17 cases of recurrence and 7 casesof death. In 23 cases of medulloblastoma in children with age of3 years old or above, 2 cases who underwent surgical removal ofintracranial ependymoma received small dosage of postoperativeX-ray radiotherapy on th

关键词： tumor of posterior cranial fossa surgicaI treatment children.

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例析高考生物学试题中的科学探究核心素养

生物学教学 2020年第2期45卷 51-54页

作者：王魏然北京市十一学校

科学探究是2017版课程标准明确提出的核心素养之一。以2018年北京高考理综29题为例,提炼高考生物学试题中科学探究的基本思路;以近年高考题和北京各区县模拟题为例,整理基本流程外的多种变式。通过对以上规律的总结提高学生对此类试题... 详细信息

科学探究是2017版课程标准明确提出的核心素养之一。以2018年北京高考理综29题为例,提炼高考生物学试题中科学探究的基本思路;以近年高考题和北京各区县模拟题为例,整理基本流程外的多种变式。通过对以上规律的总结提高学生对此类试题的把握和解答能力并提升其科学探究等核心素养。

关键词：高考生物学试题科学探究核心素养

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面向服务的虚拟企业模型及其一致性检查机制

高技术通讯 2011年第10期21卷 1076-1083页

作者：王伟然范玉顺清华大学自动化系北京100084

针对面向服务的虚拟企业业务运作对企业建模的需求，提出了面向服务的虚拟企业模型（SOVEM），并研究了其一致性检查机制。在分析面向服务的虚拟企业业务模式及其特点的基础上，提出了SOVEM的概念模型和形式化定义，讨论了其建模过程和... 详细信息

针对面向服务的虚拟企业业务运作对企业建模的需求，提出了面向服务的虚拟企业模型（SOVEM），并研究了其一致性检查机制。在分析面向服务的虚拟企业业务模式及其特点的基础上，提出了SOVEM的概念模型和形式化定义，讨论了其建模过程和成员企业模型映射方法，继而讨论了模型的一致性检查机制，定义了一致性约束并给出了模型验证算法，以保证模型的有效性，并且通过面向服务的虚拟企业建模原型系统的实现和实例检验验证了SOVEM及其一致性检查机制的可行性和有效性。该模型着眼于面向服务的业务模式，反映了网络化制造的特点，可为面向服务的虚拟企业提供一致的、全范围、多视图的集成化描述，以支持其构建和运行。

关键词：面向服务的企业(SOE) 虚拟企业企业模型服务建模一致性

IMPLEMENTING SECURITY FOR ACTIVE NETWORKS IN INTERNET

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Journal of Electronics(China) 2003年第4期20卷 305-308页

作者： Tang Yin wang weiran (College of Electronic Engineering, UEST of China, Chengdu 610054) College of Electronic Engineering UEST of China Chengdu

Active networks is primarily a Defense Advanced Research Projects Agency(DARPA)-funded project focusing on the research of mechanisms, applications, and operating systems to develop a reconfigurable network infrastructure. This letter proposes an Secure Active Tracing System (SATS) to implementing security for active networking in Internet. Unlike currently existing schemes, SATS reduces the computational overloads by executing the filtering operation on selected packet streams only when needed.

关键词： Active networks Security Distributed Denial of Service (DDoS) Active network Backbone(ABone) Active Network Transport System(ANTS) Active Signaling Protocol (ASP)