miR398b negatively regulates cotton immune responses to Verticillium dahliae via multiple targets

作     者：Yuhuan Miao Kun Chen Jinwu Deng Lin Zhang Weiran Wang Jie Kong Steven J.Klosterman Xianlong Zhang Alifu Aierxi Longfu Zhu Yuhuan Miao;Kun Chen;Jinwu Deng;Lin Zhang;Weiran Wang;Jie Kong;Steven J.Klosterman;Xianlong Zhang;Alifu Aierxi;Longfu Zhu

作者机构：National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhan 430070HubeiChina Hubei University of Chinese MedicineWuhan 430065HubeiChina Oil Crops Research InstituteChinese Academy of Agricultural SciencesWuhan 430062HubeiChina Institute of Economic CropsXinjiang Academy of Agricultural SciencesUrumchi 842000XinjiangChina United States Department of AgricultureAgricultural Research ServiceSalinasCA 93905USA 

出 版 物：《The Crop Journal》 (作物学报（英文版）)

年 卷 期：2022年第10卷第4期

页      面：1026-1036页

核心收录：

学科分类：09[农学] 0904[农学-植物保护] 090401[农学-植物病理学] 090402[农学-农业昆虫与害虫防治] 

基　　金：supported by the National Key Research and Development Program of China(2018YFD0100403) the project from the Ministry of Science and Technology of China(KY201702009) 

主　　题：Cotton miR398b NBS-LRR ROS homeostasis Verticillium dahliae 

摘      要：MicroRNAs(miRNAs)play essential roles in plant defense responses,although such roles have not been identified in cotton in response to the plant pathogenic fungus Verticillium *** this study,the functions of miR398b and its target genes in *** interaction were *** transcript levels of miR398b were down-regulated by *** infection and miR398b overexpression in cotton made the plants more susceptible to *** results suggest that miR398b negatively regulates cotton resistance to *** may occur by miR398b repression of some CC-NBS-LRR genes via translational inhibition,interfering with defense responses and leading to cotton susceptibility to ***,miR398b may guide the cleavage of the mRNAs of GhCSD1,GhCSD2 and GhCCS,each of which functions in reactive oxygen species(ROS)regulation and homeostasis,thereby causing excessive ROS accumulation in miR398b-overexpressing plants in response to *** *** study suggests conserved and novel roles of miR398b in the cotton–*** *** discoveries may be coupled with new strategies in cotton breeding programs to improve resistance to ***.