Isolation of microsatellite loci and reliable genotyping using noninvasive samples of a critically endangered primate, Trachypithecus leucocephalus

作     者：Weiran WANG Yu QIAO Yitao ZHENG Meng YAO 

作者机构：School of Life SciencesPeking UniversityBeijingChina 

出 版 物：《Integrative Zoology》 (整合动物学（英文版）)

年 卷 期：2016年第11卷第4期

页      面：250-262页

核心收录：

学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学] 

基　　金：supported by the Natural Science Foundation of China(Grant No.31272329) Project 985 Research Fund to M.Y 

主　　题：Asian colobine conservation genetics FIASCO hair scatology 

摘      要：Genetic information can be critical in identifying conservation priorities and developing conservation *** is an urgent need for noninvasive genetic tools to study the wild populations of Asian colobine *** majority of these species are threatened with habitat destruction,population reduction and even extinction,but generally lack information on their genetic diversity and population *** sampling and tissue collection have been scarce in these species owing to strict regulations on manipulation of endangered species,and the difficulties and risks associated with capturing these arboreal and fast-moving monkeys in the challeng­ing environments that they *** difficulties have hindered the development of molecular genetic mark­ers,which are usually derived from tissues or *** this study,we present a method for de novo microsatel­lite isolation and genotyping using DNA from noninvasive origins of a critically endangered Asian colobine,the white-headed langur(Trachypithecus leucocephalus).Genomic DNA isolated from hair was shown to be suf­ficient for microsatellite enrichment and isolation,with similar isolation efficiencies as from tissue *** identified and characterized 20 polymorphic microsatellite loci,and evaluated their amplification success and genotyping reliability with 86 field-collected fecal *** results show that this panel of loci can pro­duce reliable genotypes from fecal samples,and represent a useful tool for noninvasive investigation of genetic structure,individual identification and kinship assessment in this highly endangered *** approach can be applied to conservation genetic studies of other wild species that lack sequence information and tissue sam­ples.