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TGR5 expression in benign, preneoplastic and neoplastic lesions of Barrett's esophagus: Case series and findings

World Journal of Gastroenterology 2017年第8期23卷 1338-1344页

作者： Shivali Marketkar Dan Li Dongfang Yang Weibiao Cao Department of Pathology Rhode Island Hospital and Warren Alpert Medical School of Brown University Department of Medicine Rhode Island Hospital and Warren Alpert Medical School of Brown University

AIM To examined the bile acid receptor TGR5 expression in squamous mucosa, Barrett's mucosa, dysplasia and esophageal adenocarcinoma(EA). METHODS Slides were stained with TGR5 antibody. The staining intensity was scored as 1+, 2+ and 3+. The extent of staining(percentage of cells staining) was scored as follows: 1+, 1%-10%, 2+, 11%-50%, 3+, 51%-100%. A combined score of intensity and extent was calculated and categorized as negative, weak, moderate and strong staining. TGR5 m RNA was measured by real time PCR.RESULTS We found that levels of TGR5 mR NA were significantly increased in Barrett's dysplastic cell line CP-D and EA cell line SK-GT-4, when compared with Barrett's cell line CP-A. Moderate to strong TGR5 staining was significantly higher in high-grade dysplasia and EA cases than in Barrett's esophagus(BE) or in low-grade dysplasia. Moderate to strong staining was slightly higher in low-grade dysplasia than in BE mucosa, but there is no statistical significance. TGR5 staining had no significant difference between high-grade dysplasia and EA. In addition, TGR5 staining intensity was not associated with the clinical stage, the pathological stage and the status of lymph node metastasis.CONCLUSION We conclude that TGR5 immunostaining was much stronger in high-grade dysplasia and EA than in BE mucosa or low-grade dysplasia and that its staining intensity was not associated with the clinical stage, the pathological stage and the status of lymph node metastasis. TGR5 might be a potential marker for the progression from BE to high-grade dysplasia and EA.

关键词： TGR5 食道的腺癌胆汁酸

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大直径盾构隧道新型纵缝接头抗弯性能试验研究

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地下空间与工程学报 2024年第2期20卷 497-506页

作者：柳献师一鸣胡秋斌曹伟飚李文勇同济大学土木工程学院上海200092 上海申通地铁集团有限公司上海201102

纵缝接头是盾构隧道受力性能的薄弱部位,管片接头的形式直接影响盾构隧道的力学性能。目前国内的盾构隧道纵缝接头使用的连接件多为螺栓,新型纵缝接头使用了一种新的连接件—滑入式连接件,此种接头在大直径盾构隧道中的受力性能有待研... 详细信息

纵缝接头是盾构隧道受力性能的薄弱部位,管片接头的形式直接影响盾构隧道的力学性能。目前国内的盾构隧道纵缝接头使用的连接件多为螺栓,新型纵缝接头使用了一种新的连接件—滑入式连接件,此种接头在大直径盾构隧道中的受力性能有待研究。本文以新型纵缝接头为研究对象,针对两种不同型号的滑入式连接件,采用模型试验的方法探究了大直径盾构隧道新型纵缝接头的受力性能,通过理论分析计算了新型纵缝接头的极限承载力,并比较分析了传统螺栓纵缝接头和新型纵缝接头的受力性能。结果表明:新型接头衬砌管片的薄弱部位在连接件周围的混凝土区域;滑入式连接件的型号直接影响新型纵缝接头的受力性能,两种接头中连接件尺寸较大的接头转角刚度相较于连接件尺寸较小的接头转角刚度增加了7.2~169.5%,极限承载能力增加了69.9%;新型纵缝接头比螺栓接头有更高的转角刚度,受力性能更好,适用于大直径盾构隧道。

关键词：盾构隧道管片接头滑入式连接件抗弯刚度受力性能

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Application of SPE-GC/MS in resolution of metabolism pattern of higher alcohols in rat plasma

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Food Science and Human Wellness 2023年

作者： Yufei Liu Xiaonian cao Zhilei Zhou Qingxi Ren Zhongwei Ji Min Gong Yuezheng Xu weibiao Zhou Shuguang Chen Jian Mao Department of Food Science and Technology National University of Singapore Luzhou Laojiao Co. Ltd. Department of General Surgery Peking Union Medical College Hospital Chinese Academy of Medical Sciences and Peking Union Medical College (CAMS & PUMC) Shaoxing Key Laboratory of Traditional Fermentation Food and Human Health Jiangnan University (Shaoxing) Industrial Technology Research Institute Jiangsu Provincial Engineering Research Center for Bioactive Product Processing Jiangnan University National Engineering Research Center of Cereal Fermentation and Food Biomanufacturing School of Food Science and Technology Jiangnan University National Engineering Research Center for Huangjiu Zhejiang Guyuelongshan Shaoxing Wine Co. Ltd Zhejiang Shaoxing Huangjiu Industry Innovation Service Complex

Higher alcohols are key factors affecting sensory quality and post-drinking comfort of alcoholic beverages. A strategy combining solid-phase extraction and gas chromatography-mass spectrometry (SPE-GC/MS) was established to analyze the metabolism pattern of higher alcohols in rat plasma after gavage of four common alcoholic beverages including huangjiu， baijiu， wine and brandy. Seven milliliter of dichloromethane was determined as the optimal extraction condition， and eight higher alcohols were precisely quantified with detection limits of 1.82-11.65 μg/L， recoveries of 89.07%-110.89% and fine repeatability. The fastest absorption and elimination rates of plasma total higher alcohols were observed in baijiu and huangjiu group， respectively， and the highest peak concentration was found in brandy group. Additionally， the metabolic rate of plasma isoamyl alcohol in huangjiu group was faster than that in wine group at the same intragastric administration dosage. This study may provide potential insight for evaluation of alcoholic beverage quality.

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采用预埋承插式接头的大直径盾构管片设计方案研究

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隧道与轨道交通 2023年第4期 46-50,70页

作者：蒋征张旭 cao weibiao 上海申通地铁建设集团有限公司上海市隧道工程轨道交通设计研究院不详

目前盾构法隧道普遍使用装配式管片作为隧道的承载结构,而接头连接件形式是装配式衬砌的关键。螺栓作为连接件安装直观可靠,可以保证要求的紧固力;但螺栓手孔对管片结构造成削弱,易引起孔边开裂,进而影响衬砌结构整体耐久性;螺栓的紧固... 详细信息

目前盾构法隧道普遍使用装配式管片作为隧道的承载结构,而接头连接件形式是装配式衬砌的关键。螺栓作为连接件安装直观可靠,可以保证要求的紧固力;但螺栓手孔对管片结构造成削弱,易引起孔边开裂,进而影响衬砌结构整体耐久性;螺栓的紧固依赖人工作业,施工质量受人为因素影响大,易造成错台,影响成型隧道质量。2019年,上海轨道交通18号线出入段线工程首次在外径为?6.6 m的盾构法区间隧道中试验性地应用了预埋承插式接头管片,隧道成型后质量优良,基本无病害,有效地降低了后期运营维护压力和成本。为了将预埋承插式接头管片进行扩展应用,依托上海轨道交通崇明线越江区间工程,开展了大直径隧道管片接头试验研究,探索了大直径过江隧道采用预埋承插式接头管片的设计新思路。

关键词：盾构法隧道预埋承插式连接件自动化接头受力性能

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Absence of FHIT expression is associated with apoptosis inhibition in colorectal cancer

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Frontiers of Medicine 2007年第2期1卷 147-156页

作者： cao Jie CHEN Xiaoping LI Wanglin XIA Jie DU Hong TANG weibiao CHEN Shanming WANG Hui CHEN Xiwen XIAO Huanqing LI Yuyuan Department of Gastrointestinal Surgery Affiliated Guangzhou First People’s HospitalGuangzhou Medical CollegeGuangzhou 510180China Center of Hepatic Surgery Tongji HospitalTongji Medical CollegeHuazhong University of Science and TechnologyWuhan 430030China Department of Pathology Affiliated Guangzhou First People’s HospitalGuangzhou Medical CollegeGuangzhou 510180China Department of Scientific Research and Education Affiliated Guangzhou First People’s HospitalGuangzhou Medical CollegeGuangzhou 510180China Department of Gastroenterology Affiliated Guangzhou First People’s HospitalGuangzhou Medical CollegeGuangzhou 510180China

The fragile histidine triad(FHIT)gene,a candidate tumor suppressor gene located at 3p14.2,has been shown to be involved in the carcinogenesis of many human tissues,including digestive tract tissues.However,the expression and the role of the FHIT in the initiation and the development of the colorectal cancer(CRC)are poorly understood.We have shown that the FHIT gene exhibits significantly decreased expression in human CRC compared to colorectal adenoma and normal colorectal tissue by tissue microarray(TMA).The positive rate of FHIT gene expression in normal colorectal tissue,adenoma and adenocarcinoma were 93.75%,68.75%and 46.25%,respectively.We show this decreased expression to be significantly correlated with the progression of colorectal carcinoma(P<0.05)as well as with differentiation and lymph node metastasis(P<0.05).We detected two somatic alterations in the FHIT gene in human CRC.The presence of this mutation correlated significantly with decreased FHIT expression in the human CRC.In our present study we tested the hypothesis that the decreased FHIT expression resulted in apoptosis inhibition associated with abnormal expression of apoptosis related proteins.To test this hypothesis we did a series of experiments.In the first test,we assessed apoptosis status using a standard TUNEL(terminal deoxynucleotidyl transferase-mediated dUTP nick-end-labeling)assay by comparing FHIT-positive CRC vs.FHIT-negative CRC.In the second experiment,the protein expression of the FHIT and other apoptosis related proteins(Bax,Bcl-2 and Survivin)were measured in human CRC by TMA.Our combined results demonstrate the mutation in the FHIT gene significantly reduced FHIT expression in human CRC.Both TUNEL and TMA experiments demonstrated significantly inhibited apoptosis by down-regulation of Bax and the up-regulation of Survivin and Bcl-2.Collectively,these studies identify the mechanism by which an important tumor suppressor gene,FHIT is inactivated specifically in human CRC contributing to our understanding of the mechanism of col

关键词： fragile histidine triad expression apoptosis colorectal cancer

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Absence of FHIT expression is associated with apoptosis inhibition in colorectal cancer

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The Chinese-German Journal of Clinical Oncology 2007年第1期6卷 44-51页

作者： Jie cao Xiaoping Chen Wanglin Li Jie Xia Hong Du weibiao Tang Hui Wang Xiwen Chen Huanqing Xiao Yuyuan Li Center of Hepatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Science and Technology Wuhan 430030 China Department of Gastrointestinal Surgery Affiliated Guangzhou First People＇s Hospital Guangzhou Medical College Guangzhou 510180 China Department of Pathology Affiliated Guangzhou First People＇s Hospital Guangzhou Medical College Guangzhou 510180 China Department of Gastroenterology Affiliated Guangzhou First People＇s Hospital Guangzhou Medical College Guang- zhou 510180 China

Objective: To investigate the expression of fragile histidine triad (FHIT) protein in normal colorectal tissue, colorectal adenoma and colorectal cancer (CRC), and to study the relationships between the expression of FHIT protein and the clinical pathology, the apoptosis-associated protein (Bcl-2, Bax, Survivin), apoptosis in colorectal cancer. Methods: Tissue microarray (TMA) and immunohistochemistry SP were used to detect the expression of FHIT gene, Bcl-2, Bax and Survivin in 16 cases of the normal colorectal tissue, 16 cases of colorectal adenoma and 80 cases of the colorectal cancer. TUNEL was used to detect the apoptosis index (AI) in 80 cases of the colorectal cancer. Results: (1) The positive rates of FHIT gene expression in normal colorectal tissue, colorectal adenoma and adenocancer were 93.75%, 68.75% and 46.25% respectively. There were no significant differences in the relationships between the FHIT gene expression and histological types, the gender as well as the age (P>0.05). There were significant relationships between FHIT gene expression and lymph node metastasis, histological grades, Duke’s system as well as the 5-year survival rate after operation. (2) The positive rates of Bax, Bcl-2 and Survivin in colorectal adenocancer were 72.50%, 51.25%, 77.50% respectively. The expression of FHIT gene was positively correlated with that of Bcl-2, Bax and Survivin. (3) The mean AI in FHIT negative tumors was significantly lower than that in FHIT positive tumors (P<0.01). Conclusion: FHIT gene may play a role in the oncogenesis and progression of colorectal cancer. The abnormal regulation of apoptosis may play an important role in the pathogenesis of colorectal cancer.

关键词：结直肠癌 FHIT 表达缺失凋亡抑制相关性

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地铁列车荷载作用下砂土液化动三轴试验研究

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路基工程 2023年第6期 66-72页

作者：沈张勇林想曹伟飚马险峰曹明洋上海市隧道工程轨道交通设计研究院上海200235 同济大学土木工程学院上海200092

为研究高速地铁列车穿越可液化地层情况下,采用动三轴试验的方法,探究隧道周边土体在列车运营荷载下液化的可能性问题。通过采集现场土进行试验,其中动荷载幅值大小依据现场实测振动数据建立的单洞双线通车数值模拟计算结果确定。分析... 详细信息

为研究高速地铁列车穿越可液化地层情况下,采用动三轴试验的方法,探究隧道周边土体在列车运营荷载下液化的可能性问题。通过采集现场土进行试验,其中动荷载幅值大小依据现场实测振动数据建立的单洞双线通车数值模拟计算结果确定。分析在振动荷载作用下试样孔压的发展,依据孔压与围压的相对大小关系进行液化判别,并提出处理措施建议。

关键词：地铁列车荷载隧道周围土层砂土液化围压动荷载幅值振动频率

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持续气道正压通气治疗睡眠呼吸暂停合并肺心病呼吸衰竭

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中华结核和呼吸杂志 1994年第S1期17卷 79-81页

作者：黄席珍吴全有曹伟标丁彦北京协和医院

报告6例慢性阻塞性肺疾病(COPD)合并睡眠呼吸暂停综合征(OSAS)(即重叠综合征)并发肺心病呼吸衰竭的患者,用持续气道正压(CPAP)通气6～10cmH2O (1cmH2O = 0.098kPa)治疗后,多导睡眠图各项指标及症状均有明显好转,5例带CPAP通气治疗机回... 详细信息

报告6例慢性阻塞性肺疾病(COPD)合并睡眠呼吸暂停综合征(OSAS)(即重叠综合征)并发肺心病呼吸衰竭的患者,用持续气道正压(CPAP)通气6～10cmH2O (1cmH2O = 0.098kPa)治疗后,多导睡眠图各项指标及症状均有明显好转,5例带CPAP通气治疗机回家长期坚持治疗,6例患者随诊半年～4年,5例带机回家长期治疗者,症状明显改善,3例恢复上班,2例退休在家,生活质量改善,无肺心病心力衰竭发生。另1例未长期用CPAP治疗,出院后半年肺心病心力衰竭、呼呼衰竭再次加重住院抢救。强调重叠综合征患者可能出现有更严重与睡眠有关的低氧血症和更明显的肺动脉高压,更容易发展成肺源性心脏病呼吸衰竭,用CPAP治疗可获较好的治疗效果。

关键词：持续气道正压通气睡眠呼吸暂停综合征肺疾病,阻塞性肺心病呼吸衰竭

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承插式接头管片衬砌结构受力性能现场测试研究

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隧道与轨道交通 2023年第4期 7-11,68页

作者：张明海曹伟飚柳献李文勇上海隧道工程公司上海市隧道工程轨道交通设计研究院同济大学

上海轨道交通18号线试验段(沈梅路站—工作井)盾构法区间隧道采用了承插式新型接头管片。为了解应用该新型管片的盾构法隧道衬砌结构的受力性能及变形情况,采用水土压力计和钢筋应力计对衬砌结构拼装过程中的外荷载和钢筋应力进行现场... 详细信息

上海轨道交通18号线试验段(沈梅路站—工作井)盾构法区间隧道采用了承插式新型接头管片。为了解应用该新型管片的盾构法隧道衬砌结构的受力性能及变形情况,采用水土压力计和钢筋应力计对衬砌结构拼装过程中的外荷载和钢筋应力进行现场测试。研究结果表明:一是脱出盾尾阶段外侧荷载分布不均匀,整体上试验环底部荷载较大;同步注浆工况下外荷载更均匀,注浆时各测点荷载增加比较均匀,且注浆时的荷载峰值随盾构机的推进呈减小趋势;二是管片轴力与外荷载呈正相关,而弯矩分布规律并不明显,荷载稳定后弯矩基本对称分布。

关键词：盾构法隧道承插式接头管片衬砌结构外荷载内力响应现场测试

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地铁盾构隧道复合腔体构件性能试验研究

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城市轨道交通研究 2022年第6期25卷 31-36,42页

作者：邓世舜沈张勇柳献许文曹伟飚张磊刘旭阳南京地铁运营有限责任公司南京210008 上海市隧道工程轨道交通设计研究院上海200235 同济大学地下建筑与工程系上海200092

复合腔体是一种新型的加固盾构隧道的结构材料,它能够为隧道结构提供额外的承载力,控制结构变形。对灌浆复合腔体、空腔复合腔体和空腔钢管的抗弯、抗压性能进行研究。分析了复合腔体抗压和抗弯的破坏机理,得到其受压承载力、受弯承载... 详细信息

复合腔体是一种新型的加固盾构隧道的结构材料,它能够为隧道结构提供额外的承载力,控制结构变形。对灌浆复合腔体、空腔复合腔体和空腔钢管的抗弯、抗压性能进行研究。分析了复合腔体抗压和抗弯的破坏机理,得到其受压承载力、受弯承载力和刚度的变化规律。结果表明,外层碳纤维间绷断为复合腔体构件丧失抗压、抗弯承载能力的标志;碳纤维材料的约束作用显著提高了试件的截面抗弯刚度和承载能力,但降低了构件的延性,砂浆材料显著提高了试件的抗压承载能力;碳纤维、砂浆、钢管三者共同作用效果欠佳,模拟中需考虑三者材料间的滑移。

关键词：地铁盾构隧道复合腔体构件性能试验

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