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International Journal of Agricultural and Biological Engineering 2024年第4期17卷 288-296页

作者： guanying wang Gang Zhang Xiongjun Shao State Key Laboratory of Biocatalysis and Enzyme Engineering/Hubei Key Laboratory of Industrial Biotechnology School of Life SciencesHubei UniversityWuhan 430062China

Lime pretreatment on maize stover was conducted with various pretreatment conditions selected by central composite *** and lignin contents in pretreated maize stover were relatively *** of glucose monomer and oligomers decreased while concentration of xylooligomers increased in the pretreatment hydrolysate with increasing pretreatment *** overall carbohydrate recovery was at least 85%for the conditions *** removal during pretreatment was found to have a linear correlation with lignin *** had a higher effect in enhancing carbohydrate solubilization for enzymatic hydrolysis than that for *** *** all the pretreated solids,Clostridium thermocellum fermentation was found to result in much higher carbohydrate solubilization than enzymatic hydrolysis with a cellulase loading of 8 mg/g solids and a xylanase loading of 2 mg/g *** solubilization was found to have a linear correlation with lignin removal during lime pretreatment for both enzymatic hydrolysis and *** *** the current challenges,this research provides a new idea for the industrial application of lignocellulosic biorefinery.

关键词： central composite design lime pretreatment enzymatic hydrolysis clostridium thermocellum carbohydrate solubilization

Knock-out of GhPDCT with the CRISPR/Cas9 system increases the oleic acid content in cottonseed oil

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Journal of Integrative Agriculture 2024年第10期23卷 3468-3471页

作者： Tingwan Li Lu Long Yingchao Tang Zhongping Xu guanying wang Man Jiang Shuangxia Jin Wei Gao National Key Laboratory of Cotton Bio-breeding and Integrated Utilization/School of Life Science Henan UniversityKaifeng475004China Hubei Hongshan Laboratory/National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan430070China

Cotton is a pivotal economic crop for natural textile fibers that also serves as an important source of edible oil(Long et al.2023).Cottonseed oil contains approximately14%oleic acid and 59%linoleic *** increase in monounsaturated fatty acids,particularly oleic acid,enhances the oxidative stability and nutritional value of edible oil(Chen et al.2021).

关键词： CRISPR/Cas9 fibers system

CRISPR-Cas9-mediated construction of a cotton CDPK mutant library for identification of insect-resistance genes

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Plant Communications 2024年第11期5卷 23-40页

作者： Fuqiu wang Sijia Liang guanying wang Tianyu Hu Chunyang Fu Qiongqiong wang Zhongping Xu Yibo Fan Lianlian Che Ling Min Bo Li Lu Long Wei Gao Xianlong Zhang Shuangxia Jin Hubei Hongshan Laboratory National Key Laboratory of Crop Genetic ImprovementHuazhong Agricultural UniversityWuhan 430070China Academy of Industry Innovation and Development Huanghuai UniversityZhumadianHenan 463000China Xinjiang Key Laboratory of Crop Biotechnology Institute of Nuclear and Biological TechnologyXinjiang Academy of Agricultural SciencesUrumqi 830091 XinjiangChina National Key Laboratory of Cotton Bio-breeding and Integrated Utilization School of Life ScienceHenan UniversityHenan 475004China These authors contributed equally to this article

Calcium-dependent protein kinases(CDPKs)act as key signal transduction enzymes in plants,especially in response to diverse stresses,including *** this study,a comprehensive analysis of the CDPK gene family in upland cotton revealed that GhCPKs are widely expressed in multiple cotton tissues and respond positively to various biotic and abiotic *** developed a strategy for screening insect-resistance genes from a CRISPR-Cas9 mutant library of *** library was created using 246 single-guide RNAs targeting the GhCPK gene family to generate 518 independent T0 *** average target-gene coverage was 86.18%,the genome editing rate was 89.49%,and the editing heritability was 82%.An insect bioassay in the field led to identification of 14 GhCPK mutants that are resistant or susceptible to *** mutant that showed the clearest insect resistance,cpk33/74(in which the homologous genes GhCPK33 and GhCPK74 were knocked out),was selected for further *** secretions from Spodoptera litura induced a rapid influx of Ca^(2+) in cpk33/74 leaves,resulting in a significant increase in jasmonic acid content.S-adenosylmethionine synthase is an important protein involved in plant stress response,and protein interaction experiments provided evidence for interactions of GhCPK33 and GhCPK74 with GhSAMS1 and *** addition,virus-induced gene silencing of GhSAMS1 and GhSAM2 in cotton impaired defense against *** study demonstrates an effective strategy for constructing a mutant library of a gene family in a polyploid plant species and offers valuable insights into the role of CDPKs in the interaction between plants and herbivorous insects.

关键词： cotton CDPKs mutant library CRISPR-Cas9 Ca^(2+)influx insect resistance

A near-complete genome assembly of Catharanthus roseus and insights into its vinblastine biosynthesis and high susceptibility to the Huanglongbing pathogen

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Plant Communications 2023年第6期4卷 216-220页

作者： Zhongping Xu guanying wang Qiongqiong wang Xiaoting Li Guangyu Zhang Ali Qurban Can Zhang Yi Zhou Huan Si Lisong Hu Fuqiu wang Ying wang Zhitao Tian Wei Chen Shuangxia Jin Fang Ding Hubei Key Laboratory of Plant Pathology College of Plant Science and TechnologyHuazhong Agricultural UniversityWuhanHubeiChina National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhanHubeiChina Tobacco Research Institute Chinese Academy of Agricultural SciencesQingdaoShandongChina Spice and Beverage Research Institute Chinese Academy of Tropical Agricultural SciencesWanningHainanChina National Key Laboratory of Crop Genetic Improvement and National Center of Plant Gene Research(Wuhan) Huazhong Agricultural UniversityWuhanHubeiChina Key Laboratory of Horticultural Plant Biology of MOE(Ministry of Education) Huazhong Agricultural UniversityWuhanHubeiChina

Dear Editor,Catharanthus roseus,an important medicinal plant native to Madagascar,is the sole source of the two important anticancer drugs vinblastine and vincristine,which are synthesized by the monoterpene-derived indole alkaloid(MIA)pathway(Das et al.,2020).It is also an important model plant for the study of Huanglongbing(HLB),a devastating citrus disease that causes severe economic losses worldwide(Ma et al.,2022).Vincristine is an FDA-approved drug that has been used for acute lymphocytic leukemia,lymphoid blast crisis of chronic myeloid leukemia,and Hodgkin’s/non-Hodgkin’s lymphoma(Below and M Das,2021).

关键词： drugs lymphocytic

Merging polymers of intrinsic microporosity and porous carbon-based zinc oxide composites in novel mixed matrix membranes for efficient gas separation

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Green Energy & Environment 2025年第1期10卷 203-213页

作者： Muning Chen Jiemei Zhou Jing Ma Weigang Zheng guanying Dong Xin Li Zhihong Tian Yatao Zhang Jing wang Yong wang School of Chemical Engineering Zhengzhou UniversityZhengzhou450001HenanChina State Key Laboratory of Materials-Oriented Chemical Engineering and College of Chemical Engineering Nanjing Tech UniversityNanjing211816JiangsuChina Singapore Membrane Technology Centre Nanyang Environment and Water Research InstituteNanyang Technological UniversitySingapore637141Singapore Engineering Research Center for Nanomaterials Henan UniversityKaifeng475004HenanChina State Key Laboratory of Coking Coal Resources Green Exploitation Zhengzhou UniversityZhengzhou 450001China School of Energy and Environment Southeast UniversityNanjing210096JiangsuChina

Mixed matrix membranes(MMMs)have demonstrated significant promise in energy-intensive gas separations by amalgamating the unique properties of fillers with the facile processability of ***,achieving a simultaneous enhancement of permeability and selectivity remains a formidable challenge,due to the difficulty of achieving an optimal match between polymers and *** this study,we incorporate a porous carbon-based zinc oxide composite(C@ZnO)into high-permeability polymers of intrinsic microporosity(PIMs)to fabricate *** dipole–dipole interaction between C@ZnO and PIMs ensures their exceptional compatibility,mitigating the formation of non-selective voids in the resulting ***,C@ZnO with abundant interconnected pores can provide additional low-resistance pathways for gas transport in *** a result,the CO_(2) permeability of the optimized C@ZnO/PIM-1 MMMs is elevated to 13,215 barrer,while the CO_(2)/N_(2) and CO_(2)/CH4 selectivity reached 21.5 and 14.4,respectively,substantially surpassing the 2008 Robeson upper ***,molecular simulation results further corroborate that the augmented membrane gas selectivity is attributed to the superior CO_(2) affinity of C@*** summary,we believe that this work not only expands the application of MMMs for gas separation but also heralds a paradigm shift in the application of porous carbon materials.

关键词： Mixed matrix membranes Polymers of intrinsic microporosity CO_(2)separation Porous carbon materials

A NEW KOSSEL ORIENTATION METHOD OF CRYSTAL

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Acta Metallurgica Sinica(English Letters) 1989年第9期2卷 205-209页

作者： HUANG Rong wang guanying Shanghai Institute of Metallurgy,Academia Sinica,Shanghai,China HUANG Rong Shanghai Institute of Metallurgy,Academia Sinica,Shanghai,China

A new Kossel orientation method of crystal is *** the distances among three in- tersections of the Kossel lines on the indexed Kossel patterns observed are *** de- termining the ***,the error may be eliminated and the... 详细信息

关键词： Kossel orientation method

Solution of false alarm and slow response in flame detector

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Journal of Southeast University(English Edition) 2019年第2期35卷 174-178页

作者： Song Wengang Zhang Lijun Zheng Zhanqi wang guanying Zhang Jianming Institute of Microelectronics Chinese Academy of SciencesBeijing 100029China School of Microelectronics University of Chinese Academy of SciencesBeijing 100190China Wuhan Binhu Electronic Limited Liability Company Wuhan 430205China

A new flame detector with one ultraviolet and two infrared detectors is designed. The ultraviolet detector is of rapid response(≤10 μs) while the two infrared detectors usually have a response time of more than 5 ms. The ultraviolet detector is applied to deal with the flame of large scales. When facing the flame of mid or small scales, the three detectors cooperate. Employing the high-order derivatives of the sample data of the infrared circuits to improve the sensitivity, the response speed is greatly improved. The data of the temperature sensor is used to adjust circuit parameters in real time, thus reducing the effect of temperature drift. The flame detectors are tested at different distances and the response time is as rapid as 0.65 ms. The test results show that the new flame detector has the characteristics of high speed and a low rate of false alarms.

关键词： flame detector rapid response low false alarm rate derivative

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汉悬泉置遗址出土元与子方帛书信札考释

中国国家博物馆馆刊 1998年第1期 58-61页

作者：王冠英中国历史博物馆

汉悬泉置遗址出土元与子方帛书信札考释王冠英图１１９９７年９月至１２月国家文物局委托中国历史博物馆举办《全国考古新发现精品展》，甘肃省文物考古研究所送展西汉帛书信札一件（图１）。该信札１９９０年甘肃省敦煌市甜水井汉悬泉... 详细信息

汉悬泉置遗址出土元与子方帛书信札考释王冠英图１１９９７年９月至１２月国家文物局委托中国历史博物馆举办《全国考古新发现精品展》，甘肃省文物考古研究所送展西汉帛书信札一件（图１）。该信札１９９０年甘肃省敦煌市甜水井汉悬泉置遗址出土。出土时呈纵三折、横七折...

关键词：帛书遗址悬泉敦煌丈人信札汉简居延下级军官八分书

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Ross修复理论和利率期限结构（英文）

南开大学学报（自然科学版） 2016年第2期49卷 88-102页

作者：叶露王冠英南开大学数学科学学院天津300071 浙江理工大学经济管理学院生态文明研究中心浙江杭州310018 天津大学管理与经济学部天津300072

基于Ross提出的修复理论,预测了债券市场实际收益率的分布.给出了修复理论的数学理论依据.拓展了修复理论到无界扩散过程上。证实修复理论可以应用于典型的利率模型.进一步地,分析修复理论在多维扩散过程,带跳过程的应用.

关键词： Ross修复理论离散马氏过程测度变换状态价格

基于国债收益率曲线的从属过程参数估计（英文）

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南开大学学报（自然科学版） 2015年第6期48卷 18-26页

作者：叶露王冠英南开大学数学科学学院天津300071 浙江理工大学经济管理学院生态文明研究中心浙江杭州310018 天津大学管理与经济学部天津300072

分别基于零息票债券的连续复利到期收益率(简称零息收益率)、零息票债券的半年复利平价收益率(简称平价收益率)和瞬时远期利率,对一类轨道随时间非减的Lévy过程――从属过程进行了参数估计和模型之间的比较.对于转移函数已知的从属过程... 详细信息

分别基于零息票债券的连续复利到期收益率(简称零息收益率)、零息票债券的半年复利平价收益率(简称平价收益率)和瞬时远期利率,对一类轨道随时间非减的Lévy过程――从属过程进行了参数估计和模型之间的比较.对于转移函数已知的从属过程,用极大似然估计得到了参数的估计.对于转移函数未知的从属过程,可以利用鞍点逼近的方法得到转移函数的近似表达式,从而利用极大似然函数进行参数估计.Gamma过程对三类收益率曲线的描述上优于稳定从属过程和泊松随机积分过程.相比较零息收益率和平价收益率,稳定从属过程和泊松随机积分过程在对瞬时远期利率的描述上要吻合一点.

关键词：从属过程国债收益率曲线参数估计