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Isolation of microsatellite loci and reliable genotyping using noninvasive samples of a critically endangered primate, Trachypithecus leucocephalus

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Integrative Zoology 2016年第4期11卷 250-262页

作者： weiran wang Yu QIAO Yitao ZHENG Meng YAO School of Life Sciences Peking UniversityBeijingChina

Genetic information can be critical in identifying conservation priorities and developing conservation *** is an urgent need for noninvasive genetic tools to study the wild populations of Asian colobine *** majority of these species are threatened with habitat destruction,population reduction and even extinction,but generally lack information on their genetic diversity and population *** sampling and tissue collection have been scarce in these species owing to strict regulations on manipulation of endangered species,and the difficulties and risks associated with capturing these arboreal and fast-moving monkeys in the challenging environments that they *** difficulties have hindered the development of molecular genetic markers,which are usually derived from tissues or *** this study,we present a method for de novo microsatellite isolation and genotyping using DNA from noninvasive origins of a critically endangered Asian colobine,the white-headed langur(Trachypithecus leucocephalus).Genomic DNA isolated from hair was shown to be sufficient for microsatellite enrichment and isolation,with similar isolation efficiencies as from tissue *** identified and characterized 20 polymorphic microsatellite loci,and evaluated their amplification success and genotyping reliability with 86 field-collected fecal *** results show that this panel of loci can produce reliable genotypes from fecal samples,and represent a useful tool for noninvasive investigation of genetic structure,individual identification and kinship assessment in this highly endangered *** approach can be applied to conservation genetic studies of other wild species that lack sequence information and tissue samples.

关键词： Asian colobine conservation genetics FIASCO hair scatology

Effects of L-lactic acid and D,L-lactic acid on viability and osteogenic differentiation of mesenchymal stem cells

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Chinese Science Bulletin 2013年第20期58卷 2404-2412,1-3页

作者： HE Yao wang weiran DING JianDong State Key Laboratory of Molecular Engineering of Polymers Department of Macromolecular Science Advanced Materials Laboratory Fudan University

Poly(lactic acid) (PLA) and other aliphatic polyesters containing the unit of lactic acid are very popular biodegradable materials. While the degradation products, lactic acids, have been worried to bring with negative influence on biocompatibility, the focused experimental studies are less reported. This study is aimed at an in vitro examination of cytotoxicity of both L-lactic acid and D,L-lactic acid. Mesenchymal stem cells (MSCs) derived from rat bone marrow are employed to test the cytotoxicity of the lactic acids. Considering that the addition of lactic acids not only introduces lactate groups but also alters medium pH and ion strength, these three candidate effects are examined in a decoupled way by setting different comparison groups. The results confirm that the change of medium pH is the predominant factor. It has also been found that D-lactate is more cytotoxic than L-lactate at high concentrations. Yet, either L-or D,L-lactic acids seem acceptable in most of medical applications, because the cytotoxicity is significant only when the concentrations are as high as 20 mmol/L for both of them.

关键词：骨髓间充质干细胞 L-乳酸成骨分化细胞毒作用能力细胞毒性可生物降解生物相容性

基于连续-离散耦合方法的隧道围岩变形破坏分析

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兰州工业学院学报 2025年第1期32卷 35-40页

作者：刘先声刘洋王蔚然董瑾玥安徽建筑大学安徽省城市建设和地下空间工程技术研究中心安徽合肥230601 安徽建筑大学土木工程学院安徽合肥230601

隧道开挖后围岩的变形破坏机理对设计和施工具有重要意义。基于有限差分理论和颗粒流理论,建立二维平面隧道连续-离散耦合数值模型进行分析,分别利用FLAC^(2D)和PFC^(2D)构建连续区域与非连续区域,研究不同围压条件下隧道围岩的变形特... 详细信息

隧道开挖后围岩的变形破坏机理对设计和施工具有重要意义。基于有限差分理论和颗粒流理论,建立二维平面隧道连续-离散耦合数值模型进行分析,分别利用FLAC^(2D)和PFC^(2D)构建连续区域与非连续区域,研究不同围压条件下隧道围岩的变形特性及破坏机制。结果表明:随着地应力的增长,围岩的变形逐渐加剧,裂纹数量逐渐增多,裂纹的产生先快后慢,从8 MPa到10 MPa地应力条件下的裂纹数量有一个突变的过程,断裂和塌陷主要集中在隧道顶部;围岩的变形和裂纹数量随侧压力系数的增大而增大,当侧压力系数小于1.0时,围岩破坏程度较轻,变形主要集中在隧道两侧;当侧压力系数大于1.0时,围岩的变形程度以及裂纹数量均出现明显增长,破坏主要集中在顶板和底板。

关键词：连续-离散耦合不同围压破坏裂纹数量

co-Condensation Synthesis of Salicylaldimine Calcium Complex Containing Mesoporous Silica Nanoparticles as Carriers for Drug Release

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Chemical Research in Chinese Universities 2014年第4期30卷 531-537页

作者： TANG Duihai ZHANG weiran wang Yifan MIAO Jing QIAO Zhen'an HUO Qisheng ZHANG Lirong State Key Laboratory of Inorganic Synthesis and Preparative Chemistry College of Chemistry Jilin University Changchun 130012 P. R. China

A series of functional mesoporous silica nanoparticles（MSNs） was synthesized by a one-step simple synthesis approach involving co-condensation of tetraethoxysilane（TEOS） and salicylaldimine ligand（Sal-Si） in the presence of cetyltrimethylammonium chloride（CTAC） under basic *** target MSNs with different sizes （50,100 and 200 nm,respectively） were ***,the Ca^2＋ cations were also introduced into *** prepared nanoparticles were characterized by means of infrared（IR） spectra,thermogravimetric analysis（TGA）,inductively coupled plasma（ICP）,CHN elemental analysis,nitrogen adsorption-desorption,scanning electron microscope（SEM） and transmission electron microscope（TEM）.Ibuprofen（IBU） which contains carboxyl groups was selected as a model *** results of drug loading and release reveal that the loading capacities and release behaviors of the model drug are highly dependent on the Ca^2＋ cations in *** release of IBU from the MSNs functionalized by Ca^2＋ cations is found to be effectively controlled when compared to the release from the MSNs without the functionalization of Ca^2＋ cations,which is due to the ionic interaction between carboxyl groups in IBU and Ca^2＋ cations in MSNs.

关键词： Mesoporous silica nanoparticle co-Condensation method Release of ibuprofen Calcium ion

Attenuation of ethylene signaling increases cotton resistance to a defoliating strain of Verticillium dahliae

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The Crop Journal 2023年第1期11卷 89-98页

作者： Tianyi wang Muhammad Shaban Junhui Shi weiran wang Shiming Liu Xinhui Nie Yu Yu Jie Kong Steven J.Klosterman Xianlong Zhang Alifu Aierxi Longfu Zhu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Department of Plant Breeding and Genetics University of Agricu Institute of Economic Crops Xinjiang Academy of Agricultural SciencesKuqa 842000XinjiangChina Key Laboratory of Oasis Eco-agriculture of the Xinjiang Production and Construction Crops College of AgronomyShihezi UniversityShihezi 832000XinjiangChina Xinjiang Academy of Agricultural&Reclamation Sciences Shihezi 832000XinjiangChina United States Department of Agriculture Agricultural Research ServiceSalinasCA 93905USA

The severity of Verticillium wilt on cotton caused by defoliating strains of Verticillium dahliae has gradually increased and threatens production worldwide. Identification of the molecular components of leaf defoliation may increase cotton tolerance to V. dahliae. Ethylene, a major player in plant physiological processes, is often associated with senescence and defoliation of plants. We investigated the cotton–*** interaction with a focus on the role of ethylene in defoliation and defense against V. *** plants inoculated with V. dahliae isolate V991, a defoliating strain, accumulated more ethylene and showed increased disease symptoms than those inoculated with a non-defoliating strain. In cotton with a transiently silenced ethylene synthesis gene(GhACOs) and signaling gene(GhEINs) during cotton–V. dahliae interaction, ethylene produced was derived from cotton and more ethylene increased cotton susceptibility and defoliation rate. Overexpression of AtCTR1, a negative regulator in ethylene signaling, in cotton reduced sensitivity to ethylene and increased plant resistance to V. ***, the results indicated precise regulation of ethylene synthesis or signaling pathways improve cotton resistant to Verticillium wilt.

关键词： Cotton Verticillium dahilae Ethylene Defoliation

miR398b negatively regulates cotton immune responses to Verticillium dahliae via multiple targets

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The Crop Journal 2022年第4期10卷 1026-1036页

作者： Yuhuan Miao Kun Chen Jinwu Deng Lin Zhang weiran wang Jie Kong Steven J.Klosterman Xianlong Zhang Alifu Aierxi Longfu Zhu National Key Laboratory of Crop Genetic Improvement Huazhong Agricultural UniversityWuhan 430070HubeiChina Hubei University of Chinese Medicine Wuhan 430065HubeiChina Oil Crops Research Institute Chinese Academy of Agricultural SciencesWuhan 430062HubeiChina Institute of Economic Crops Xinjiang Academy of Agricultural SciencesUrumchi 842000XinjiangChina United States Department of Agriculture Agricultural Research ServiceSalinasCA 93905USA

MicroRNAs(miRNAs)play essential roles in plant defense responses,although such roles have not been identified in cotton in response to the plant pathogenic fungus Verticillium *** this study,the functions of miR398b and its target genes in *** interaction were *** transcript levels of miR398b were down-regulated by *** infection and miR398b overexpression in cotton made the plants more susceptible to *** results suggest that miR398b negatively regulates cotton resistance to *** may occur by miR398b repression of some CC-NBS-LRR genes via translational inhibition,interfering with defense responses and leading to cotton susceptibility to ***,miR398b may guide the cleavage of the mRNAs of GhCSD1,GhCSD2 and GhCCS,each of which functions in reactive oxygen species(ROS)regulation and homeostasis,thereby causing excessive ROS accumulation in miR398b-overexpressing plants in response to *** *** study suggests conserved and novel roles of miR398b in the cotton–*** *** discoveries may be coupled with new strategies in cotton breeding programs to improve resistance to ***.

关键词： Cotton miR398b NBS-LRR ROS homeostasis Verticillium dahliae

The Prospects of the Eurasian Economic Union

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Contemporary International Relations 2015年第5期25卷 91-104,5页

作者： wang weiran wang Jingliang Tang Tianbo Research Center on China(Xinjiang) and the Central Asian Regional Economic Cooperation Xinjiang University of Finance and Economy the Business School Xinjiang Normal University the Finance and Tax School Hebei University of Economics and Business

Russia, after realizing that it will not be truly accepted as a member of the western world, has shifted towards promoting the integration of the Eurasian areas in order to lay the geopolitical foundations for its renewed ambition as a great power. In January 1995, the three countries of Russia, Belarus and Kazakhstan established a customs union. In March 1996, Kyrgyzstan officially announced that it would be joining the union, and the four-country customs union was established. It was aimed at coordinating the economic reform pace of the four member countries, as well as accelerating economic integration between them. In February 1999, Tajikistan also joined, and in 2000, the aforementioned five countries signed the treaty that transformed the customs union into the Eurasian Economic Community. On May 31, 2001, the Interstate Council of the Eurasian Economic Community held its first meeting in Minsk, the capital of Belarus, declaring the official founding of the Community.

关键词：经济改革联盟经济一体化经济共同体白俄罗斯吉尔吉斯斯坦哈萨克斯坦塔吉克斯坦

Nighttime reactions of a series of unsaturated alcohols with NO_(3)•:Kinetics,products and mechanisms study

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Journal of Environmental Sciences 2025年第5期151卷 331-346页

作者： Yanyong Xu Shengrui Tong weiran Li Meifang Chen Lin Hu Hailiang Zhang Sufan wang Maofa Ge State Key Laboratory for Structural Chemistry of Unstable and Stable Species Chinese Academy of Sciences Research/Education Center for Excellence in Molecular SciencesInstitute of Chemistry Chinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China College of Chemistry and Material Science Anhui Normal UniversityWuhu 241000China College of Ecology and Environment Xinjiang UniversityUrumqi 830017China

Unsaturated alcohols are a class of Biogenic volatile organic compounds(BVOCs)emitted in large quantities by plants when damaged or under adverse environmental conditions,and studies on their atmospheric degradation at night are still *** used chamber experiments to study the gas-phase reactions of three unsaturated alcohols,E-2-penten-1-ol,Z-2-hexen-1-ol and Z-3-hepten-1-ol,with NO_(3)radicals(NO_(3)•)during the *** rate constants of these reactions were(11.7±1.76)×10^(−13),(8.55±1.33)×10^(−13)and(6.08±0.47)×10^(−13)cm^(3)/(molecule·s)at 298K and 760 Torr,*** contrast,the reaction rate of similar substances with ozone was about 10^(−18)cm^(3)/(molecule·s),which indicates that the reaction with NO_(3)•is themain oxidation pathway for unsaturated alcohols at *** molecule aldehydes and ketones were the main gas-phase organic products of the reaction of three aldehydes and ketones with NO_(3)•,and the total small molecule aldehydes and ketones yields can reach between 45%-60%.They mainly originate from the breakage of alkoxy radicals,and different breakage sites determine different product *** addition,the SOA yields of the three unsaturated alcohols with NO_(3)•were 7.1%±1.0%,12.5%±1.9%and 30.0%±4.5%,respectively,whichweremuch higher than those of similarly structured substances with O_(3)or OH radicals(•OH).The results of high-resolution mass spectrometry shows that the main components of Secondary organic aerosol(SOA)of the three unsaturated alcohols are dimeric compounds containing several nitrate groups,which are formed through the polymerization of oxyalkyl radicals.

关键词： Unsaturated alcohols Reaction rate Aldehyde and ketone products Secondary organic aerosol components Reaction mechanism

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改进YOLOX的轻量级多方向车牌检测算法

计算机工程与应用 2025年第4期61卷 230-240页

作者：雷景生章志豪钱小鸿王巍然杨胜英浙江科技学院信息与电子工程学院国网上海市电力公司信息通信公司

针对现有的车牌检测算法在复杂环境下检测多方向车牌效果不佳、实时性差以及模型参数量和计算量过大等问题，提出了一种基于YOLOX的轻量级多方向车牌检测算法。通过调整残差组件的数量，并采用大卷积核结合深度可分离卷积的方法，降低... 详细信息

针对现有的车牌检测算法在复杂环境下检测多方向车牌效果不佳、实时性差以及模型参数量和计算量过大等问题，提出了一种基于YOLOX的轻量级多方向车牌检测算法。通过调整残差组件的数量，并采用大卷积核结合深度可分离卷积的方法，降低了主干网络的参数量。引入通道注意力机制，以有效提取通道交互信息，减少噪声的干扰。使用深度可分离卷积和调整扩展率的方法对特征融合网络进行轻量化处理。设计了旋转解耦头，通过添加角度预测分支，使其能够更精准地预测多方向车牌的旋转边界框。采用旋转IoU损失代替水平IoU损失，提高检测的准确性。CCPD数据集上的实验结果表明，改进算法的参数量和计算复杂度分别为2.38×106和12.97 GFLOPs，相较于YOLOX-tiny分别减少了45%和33%，检测精度AP70为94.9%，每秒检测帧数为76.6 FPS。改进后的车牌检测模型能够在保持高精度的同时实时检测多方向车牌。

关键词：深度学习轻量化 YOLOX 目标检测注意力机制