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Neural stem cell-derived exosomes regulate cell proliferation,migration,and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia

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Animal Models and Experimental Medicine 2024年第1期7卷 24-35页

作者： Xiaojun Deng Xiaoyi Hu shang wang Hui Zhao Yaqin Wei Jiaqi Fu Wenhui Wu Jinming Liu Caicai Zhang Lili wang Ping Yuan Department of Critical Care Medicine Shanghai Sixth People's HospitalShanghai Jiao Tong UniversityShanghaiChina Department of Cardio-Pulmonary Circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Institute of Bismuth Science University of Shanghai for Science and TechnologyShanghaiChina Department of Physiology Hainan Medical University HaikouHainanChina Department of Clinical Medical Laboratory Center Hangzhou Red Cross HospitalZhejiangChina

Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 ***,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain ***,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 ***:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on ***:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic *** overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic ***,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell ***1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell *** structures were observed in the regions of the hippocampus and cortex in hypoxia-induced ***,EXO treatment improved cerebrovascular ***:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic ***,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.

关键词： brain microvascular endothelial cells exosomes Hes1 miR-9 neural stem cells

CircPMS1 promotes proliferation of pulmonary artery smooth muscle cells,pulmonary microvascular endothelial cells,and pericytes under hypoxia

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Animal Models and Experimental Medicine 2024年第3期7卷 310-323页

作者： Xiaoyi Hu shang wang Hui Zhao Yaqin Wei Ruowang Duan Rong Jiang Wenhui Wu Qinhua Zhao Sugang Gong Lan wang Jinming Liu Ping Yuan Department of Cardio-Pulmonary Circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Institute of Bismuth Science University of Shanghai for Science and TechnologyShanghaiChina Department of Geriatrics Shanghai Institute of GeriatricsHuadong HospitalFudan UniversityShanghaiChina Department of Anesthesiology Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina

Background:Circular RNAs(circRNAs)have been recognized as significant regulators of pulmonary hypertension(PH);however,the differential expression and function of circRNAs in different vascular cells under hypoxia remain ***,we identified co-differentially expressed circRNAs and determined their putative roles in the proliferation of pulmonary artery smooth muscle cells(PASMCs),pulmonary microvascular endothelial cells(PMECs),and pericytes(PCs)under ***:Whole transcriptome sequencing was performed to analyze the differential expression of circRNAs in three different vascular cell *** analysis was used to predict their putative biological *** real-time polymerase chain reaction,Cell Counting Kit-8,and EdU Cell Proliferation assays were carried out to determine the role of circular postmeiotic segregation 1(circPMS1)as well as its potential sponge mechanism in PASMCs,PMECs,and ***:PASMCs,PMECs,and PCs exhibited 16,99,and 31 differentially expressed circRNAs under hypoxia,***1 was upregulated in PASMCs,PMECs,and PCs under hypoxia and enhanced the proliferation of vascular ***1may upregulate DEP domain containing 1(DEPDC1)and RNA polymerase II subunit D expression by targeting microRNA-432-5p(miR-432-5p)in PASMCs,upregulate MAX interactor 1(MXI1)expression by targeting miR-433-3p in PMECs,and upregulate zinc finger AN1-type containing 5(ZFAND5)expression by targeting miR-3613-5p in ***:Our results suggest that circPMS1 promotes cell proliferation through the miR-432-5p/DEPDC1 or miR-432-5p/POL2D axis in PASMCs,through the miR-433-3p/MXI1 axis in PMECs,and through the miR-3613-5p/ZFAND5 axis in PCs,which provides putative targets for the early diagnosis and treatment of PH.

关键词： circular postmeiotic segregation 1 circular RNAs hypoxia pulmonary hypertension vascular cells

Comparing the efficacy and safety of low,medium,and high dosages of selexipag for treating pulmonary hypertension:A systematic review and meta-analysis

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Animal Models and Experimental Medicine 2024年第1期7卷 56-70页

作者： shang wang Yi Yan Jian Zhang Ping Yuan Ci-Jun Luo Hong-Ling Qiu Hui-Ting Li Jian Xu Lan wang Tian-Lan Li Rong Jiang Department of Cardio-Pulmonary Circulation Shanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Heart Center and shanghai Institute of Pediatric Congenital Heart Disease Shanghai Children's Medical CenterNational Children's Medical CenterShanghai Jiaotong University School of MedicineShanghaiChina Department of Respiratory and Critical Care Medicine The 416 Hospital of Nuclear IndustryThe Second Affiliated Hospital of Chengdu Medical CollegeChengduChina Department of Hematology The Affiliated Hospital of Qingdao UniversityQingdaoChina

Background:The maintenance dosage of selexipag is categorized as low,medium or *** order to assess the efficacy and safety of different dosages of selexipag for the risk stratification of pulmonary arterial hypertension(PAH),we performed a sys-tematic review and ***:Studies assessing PAH risk stratification indices,such as the World Health Organization functional class(WHO-FC),six-minute walk distance(6MWD),N-terminal pro-B-type natriuretic peptide(NT-proBNP)level,right atrial pressure(RAP),cardiac index(CI)and mixed venous oxygen saturation(SvO2),were ***:Thirteen studies were *** led to improvements in the 6MWD(MD:24.20 m,95%CI:10.74-37.67),NT-proBNP(SMD:-0.41,95%CI:-0.79-0.04),CI(MD:0.47 L/min/m^(2),95%CI:0.17-0.77)and WHO-FC(OR:0.564,95%CI:0.457-0.697).Subgroup analysis demonstrated that all three dosages improved the 6MWD.A moderate dosage led to improvements in the CI(MD:0.30 L/min/m^(2),95%CI:0.15-0.46)and WHO-FC(OR:0.589,95%CI:0.376-0.922).Within 6 months of treatment,only the WHO-FC and CI were significantly improved(OR:0.614,95%CI:0.380-0.993;MD:0.30 L/min/m^(2),95%CI:0.16-0.45,respectively).More than 6 months of treatment significantly improved the 6MWD,WHO-FC and NT-proBNP(MD:40.87 m,95%CI:10.97-70.77;OR:0.557,95%CI:0.440-0.705;SMD:-0.61,95%CI:-1.17-0.05,respectively).Conclusions:Low,medium,and high dosages of selexipag all exhibited good *** treatment lasted for more than 6 months,selexipag exerted obvious effects,even in the low-dosage *** finding is important for guiding individualized treatments.

关键词： individualized treatments meta-analysis prostacyclin receptor agonist risk stratification systematic review

干湿-冻融循环下黄土力学特性及损伤机制研究

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岩土力学 2024年第4期45卷 1092-1102页

作者：郅彬王尚杰西安科技大学建筑与土木工程学院陕西西安710054

为深入研究干湿-冻融循环对原状黄土力学特性的影响及细观损伤演化规律,通过不同次数干湿-冻融循环条件下的固结排水三轴剪切试验(consolidation drainage triaxial sheartest,CD)和核磁共振试验,从宏-细观角度分析其应力-应变曲线及强... 详细信息

为深入研究干湿-冻融循环对原状黄土力学特性的影响及细观损伤演化规律,通过不同次数干湿-冻融循环条件下的固结排水三轴剪切试验(consolidation drainage triaxial sheartest,CD)和核磁共振试验,从宏-细观角度分析其应力-应变曲线及强度指标变化规律和细观孔隙的损伤变化规律。在此基础之上,假定黄土微元强度分布服从复合函数,建立了黄土的损伤统计本构模型,并验证其适用性。研究结果表明:土体的应力-应变曲线表现为应变软化,并且随着循环次数的增大软化程度逐渐减弱。偏应力峰值随循环次数减小并逐渐趋于稳定,在循环2次时其衰减程度最大,不同围压下分别衰减了17.6%、23.2%、24.5%和18.1%。土体内胶结块在循环作用下发生破损,使得内部孔隙面积逐渐增大,主要为小孔隙向中大孔隙的转变,随着循环次数的增大,内部结构逐渐趋于稳定。

关键词：干湿-冻融循环黄土力学特性细观损伤损伤统计本构模型

基于FEM的引力参考传感器自引力计算与补偿

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中国空间科学技术（中英文） 2024年第2期44卷 89-97页

作者：高志勇王上王智国科大杭州高等研究院基础物理与数学科学学院杭州310012 中国科学院大学北京100049 中国科学院长春光学精密机械与物理研究所长春130033

空间引力波探测太极计划将利用激光干涉的方法,测量两个检验质量之间的距离变化反演引力波信息。在0.1 mHz处,要求检验质量在敏感轴方向的总残余加速度保持在3×10^(-15)m·s^(-2)/√Hz以下。由航天器载荷静引力、热形变和质量波动引起... 详细信息

空间引力波探测太极计划将利用激光干涉的方法,测量两个检验质量之间的距离变化反演引力波信息。在0.1 mHz处,要求检验质量在敏感轴方向的总残余加速度保持在3×10^(-15)m·s^(-2)/√Hz以下。由航天器载荷静引力、热形变和质量波动引起的自引力噪声是检验质量的残余加速度噪声主要来源之一,要求检验质量在敏感轴方向的自引力加速度小于1×10^(-10)m/s^(2),引力梯度小于5×10^(-8)s^(-2)。为了计算检验质量处的自引力大小和引力梯度,针对检验质量与引力源几何形状的不规则性,基于有限元法编写程序计算了引力参考传感器中的引力源作用在检验质量上的线加速度、角加速度和引力梯度。为了缩短计算时间,提出“类自适应”网格划分方法以减小网格数量,并设计了配重以补偿自引力。计算结果显示,经过补偿后的检验质量在敏感轴方向的自引力加速度为9.2377×10^(-12)m/s^(2),引力梯度为-2.5691×10^(-8)s^(-2),满足设计要求。本研究能够为航天器和引力参考传感器的设计与引力补偿提供参考与指导。

关键词：空间引力波探测引力参考传感器自引力引力梯度引力补偿有限元法

Advancements in research on transcutaneous electrical acupoint stimulation for alleviating pelvic pain in women

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Gynecology and Obstetrics Clinical Medicine 2024年第1期4卷 65-72页

作者： YI Ling wang Xin Lei Lu shang wang Xiao Hong Chang Rong Zhang Hong Lan Zhu Department of Obstetrics and Gynecology Peking University People's HospitalBeijingChina Peking University Neuroscience Research Institute BeijingChina Department of Neurobiology Peking University School of Basic Medical SciencesBeijingChina

Background Pelvicpain isacommon complaint of discomfort in women,with an increasing incidence and seriously affecting patients'quality of *** etiology involves multiple systems and disciplines,and therefore treatment modalities are *** electrical acupoint stimulation(TEAS)therapy is a classic analgesic means,and electrical stimulation of local acupoints can *** to discuss the application of TEAS for the treatment of various types of pelvic pain,the selection of optimal parameters and the renewal of *** Result TEAS fortreatmentof pelvic pain in women has been reported mainly for endometriosis,pelvic inflammatory disease(PID),intrauterine adhesion(IUA),pelvic stasis syndrome,interstitial cystitis,primary dysmenorrhoea and other *** for parameter selection,the most used of frequency is dilatational wave,the most used stimulation intensity is 10-20 mA,and the acupoint selection should be based on the specific conditions of *** The analgesic mechanism of TEAS has not been fully elucidated,but the efficacy of the treatment is remarkable and thereare no significant adverse *** selection of the parameters for TEAS and the combinationof acupoints fordifferent pains are stillthe maindirections offutureresearch.

关键词： stimulation pain pelvic

Precise capture of circulating endometrial cells in endometriosis

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中华医学杂志(英文版) 2024年第14期137卷 2024 Apr 29页

作者： wang shang Cheng Hongyan Zhu Honglan Yu Xiaoming Ye Xue Chang Xiaohong Department of Obstetrics and Gynecology Peking University People’s Hospital Beijing China Reproductive Medicine Center Peking University People’s Hospital Beijing China

Background: Endometriosis (EM) is a complex benign gynecological disease, but it has malignant biological behavior and can invade any part of the body. Clinical manifestations include pelvic pain, dysmenorrhea, infertility, pelvic nodules, and masses. Our previous study successfully detected circulating endometrial cells (CECs) in the peripheral blood of patients with EM. The purpose of this study is to overcome the limitation of cell size in the previous microfluidic chip method, to further accurately capture CECs, understand the characteristics of these cells, and explore the relationship between CECs and the clinical course characteristics of patients with ***: Human peripheral venous blood used to detect CECs and circulating vascular endothelial cells (CVECs) was taken from EM patients (n = 34) hospitalized in the Peking University People’s Hospital. We used the subtraction enrichment and immunostaining fluorescencein situ hybridization (SE-iFISH) method to exclude the interference of red blood cells, white blood cells, and CVECs, so as to accurately capture the CECs in the peripheral blood of patients with EM. Then we clarified the size and ploidy number of chromosome 8 of CECs, and a second grouping of patients was performed based on clinical characteristics to determine the relationship between CECs and clinical course ***: The peripheral blood of 34 EM patients and 12 non-EM patients was evaluated by SE-iFISH. Overall, 34 eligible EM patients were enrolled. The results showed that the detection rates of CECs were 58.8% in EM patients and 16.7% in the control group. However, after classification according to clinical characteristics, more CECs could be detected in the peripheral blood of patients with rapidly progressive EM, with a detection rate of 94.4% (17/18). In total, 63.5% (40/63) of these cells were small cells with diameters below 5 μm, and 44.4% (28/63) were aneuploid cells. No significant association was found between the

关键词： Endometriosis Circulating endometrial cells Clinical symptoms Dysmenorrhea Circulating vascular endothelial cells

CoZn-MOF衍生多级孔取向碳载CoP及其析氢性能

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化工进展 2024年第1期43卷 447-454页

作者：汪尚姚瑶王佳董迪迪常刚刚武汉理工大学化学化工与生命科学学院湖北武汉430070 武汉东湖学院生命科学与化学学院湖北武汉430212

在“双碳”背景下,高效、清洁的氢能源成为代替传统化石能源的重要选择,而电解水产氢是开发氢能源需要攻克的关键技术之一。目前,电解水产氢催化活性较高的催化剂主要以贵金属催化剂为主,然而贵金属价格高昂、资源稀缺严重阻碍了它们在... 详细信息

在“双碳”背景下,高效、清洁的氢能源成为代替传统化石能源的重要选择,而电解水产氢是开发氢能源需要攻克的关键技术之一。目前,电解水产氢催化活性较高的催化剂主要以贵金属催化剂为主,然而贵金属价格高昂、资源稀缺严重阻碍了它们在清洁能源领域的大规模应用。因此,开发高效、耐用、廉价和地球资源丰富的电催化剂是实现电解水产氢的迫切需求。本文以无定形的锌钴双金属有机框架为前体,通过酸溶液处理制备了一种具有独特多面体结构的新型骨架材料(ZnCo-MOF),然后通过逐级碳化磷化过程,在保留原有形态的基础上得到了CoP负载的具有规则形貌的多级孔碳(H-CoP/C)。研究表明:多级孔碳作载体不仅能够负载更多活性位点,而且有利于活性位点的暴露、材料的导电性和催化过程中的传质。凭借有利的结构特征、充足的活性位点,所获得的电催化剂H-CoP/C在碱性介质中展现出优异的HER性能,驱动10mA/cm^(2)的电流密度只需168mV的过电位,优于大多数的非贵金属电催化剂。

关键词：电解水析氢 CoP 金属有机骨架多级孔碳

基于试验子结构恢复力修正的不完整边界条件混合试验方法

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工程力学 2024年第5期41卷 77-86页

作者：王尚长杨格王贞吴斌肖嘉俊武汉理工大学土木工程与建筑学院湖北武汉430070 武汉理工大学三亚科教创新园海南三亚572000 中国地震局地震工程综合模拟与城乡抗震韧性重点实验室(天津大学) 天津300072

相比于传统拟静力和拟动力试验,混合试验通常存在较为复杂的试验子结构边界条件。受到实验室加载条件的限制,子结构的边界条件往往难以完全实现,这将对混合试验的保真度产生很大影响,即混合试验中的不完整边界条件问题。为解决不完整边... 详细信息

相比于传统拟静力和拟动力试验,混合试验通常存在较为复杂的试验子结构边界条件。受到实验室加载条件的限制,子结构的边界条件往往难以完全实现,这将对混合试验的保真度产生很大影响,即混合试验中的不完整边界条件问题。为解决不完整边界条件问题,提出一种基于试验子结构恢复力修正的不完整边界条件混合试验方法。该方法为试验子结构建立两套数值模型,并通过两套数值模型修正试验子结构的不完整恢复力。两套数值模型的区别在于边界条件的设定:第一套数值模型具有完整的边界条件;第二套数值模型具有与试验子结构相同的边界条件,为不完整边界条件。该研究分析了边界自由度耦合程度、数值模型误差对该方法精度的影响,验证了该方法对存在模型误差的数值模型具有较好的鲁棒性;最后,采用所提方法对一座两层两跨抗弯框架结构进行了抗震性能数值分析。结果表明:与常规混合试验方法和弱耦合混合试验方法相比,该方法不仅具有解决不完整边界条件问题的能力,而且也表现出较高的仿真精度。

关键词：混合试验不完整边界条件试验子结构恢复力修正辅助数值模型数值模拟