Neural stem cell-derived exosomes regulate cell proliferation,migration,and cell death of brain microvascular endothelial cells via the miR-9/Hes1 axis under hypoxia

作     者：Xiaojun Deng Xiaoyi Hu Shang Wang Hui Zhao Yaqin Wei Jiaqi Fu Wenhui Wu Jinming Liu Caicai Zhang Lili Wang Ping Yuan Xiaojun Deng;Xiaoyi Hu;Shang Wang;Hui Zhao;Yaqin Wei;Jiaqi Fu;Wenhui Wu;Jinming Liu;Caicai Zhang;Lili Wang;Ping Yuan

作者机构：Department of Critical Care MedicineShanghai Sixth People's HospitalShanghai Jiao Tong UniversityShanghaiChina Department of Cardio-Pulmonary CirculationShanghai Pulmonary HospitalSchool of MedicineTongji UniversityShanghaiChina Institute of Bismuth ScienceUniversity of Shanghai for Science and TechnologyShanghaiChina Department of PhysiologyHainan Medical University HaikouHainanChina Department of Clinical Medical Laboratory CenterHangzhou Red Cross HospitalZhejiangChina 

出 版 物：《Animal Models and Experimental Medicine》 (动物模型与实验医学（英文）)

年 卷 期：2024年第7卷第1期

页      面：24-35页

核心收录：

学科分类：10[医学] 

基　　金：Program of Natural Science Foundation of Shanghai,Grant/Award Number:21ZR1453800 and 22ZR1452400 Program of National Natural Science Foundation of China,Grant/Award Number:82370057 Fundamental Research Funds for the Central Universities,Grant/Award Number:22120220562 Program of Shanghai Municipal Health Commission,Grant/Award Number:20204Y0384 Program of National Key Research and Development Project of China,Grant/Award Number:2023YFC2509500 

主　　题：brain microvascular endothelial cells exosomes Hes1 miR-9 neural stem cells 

摘      要：Background:Our previous study found that mouse embryonic neural stem cell(NSC)-derived exosomes(EXOs)regulated NSC differentiation via the miR-9/Hes1 ***,the effects of EXOs on brain microvascular endothelial cell(BMEC)dysfunction via the miR-9/Hes1 axis remain ***,the current study aimed to determine the effects of EXOs on BMEC proliferation,migration,and death via the miR-9/Hes1 ***:Immunofluorescence,quantitative real-time polymerase chain reaction,cell counting kit-8 assay,wound healing assay,calcein-acetoxymethyl/propidium iodide staining,and hematoxylin and eosin staining were used to determine the role and mechanism of EXOs on ***:EXOs promoted BMEC proliferation and migration and reduced cell death under hypoxic *** overexpression of miR-9 promoted BMEC prolifera-tion and migration and reduced cell death under hypoxic ***,miR-9 downregulation inhibited BMEC proliferation and migration and also promoted cell ***1 silencing ameliorated the effect of amtagomiR-9 on BMEC proliferation and migration and cell *** structures were observed in the regions of the hippocampus and cortex in hypoxia-induced ***,EXO treatment improved cerebrovascular ***:NSC-derived EXOs can promote BMEC proliferation and migra-tion and reduce cell death via the miR-9/Hes1 axis under hypoxic ***,EXO therapeutic strategies could be considered for hypoxia-induced vascular injury.