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Open globe injury in Hospital Universiti Sains Malaysia- a 10-year review

International Journal of Ophthalmology(English edition) 2014年第3期7卷 486-490页

作者： Madhusudhan A/L Paramananda Evelyn-tai Li Min Zamri Noordin Adil Hussein wan-Hazabbah wan Hitam Department of Ophthalmology School of Medical SciencesHealth Campus Universiti Sains Malaysia Kubang KerianKelantan 16150 Malaysia Hospital Universiti Sains Malaysia Kubang KerianKelantan 16150 Malaysia

AIM:To identify the aetiology of open globe injuries at Hospital Universiti Sains Malaysia over a period of 10y and the prognostic factors for visual ***:Retrospective review of medical records of open globe injury cases that presented from January2000 to December *** of open globe injury was based on the Birmingham Eye Trauma Terminology(BETT).Records were obtained with hospital permission via the in-house electronic patient management system,and the case notes of all patients with a diagnosis of open globe injury were *** with prior ocular trauma,pre-existing ocular conditions affecting the visual acuity,contrast sensitivity,central vision or corneal thickness,as well as those with a history of previous intraocular or refractive surgery were *** of data was with SPSS *** logistic regression analysis was used to examine the association between prognostic factors and visual ***:This study involved 220 patients(n=222eyes).The most common place of injury was the home(51.8%),followed by the workplace(23.4%).Among children aged less than 16y of age,domestic-related injury was the predominant cause(54.6%),while in those aged 16y and above,occupational injuries were the most common cause(40.0%).Most eyes(76.5%)had an initial visual acuity worse than 3/60,and in half of these,the visual acuity *** visual outcome was found to be significantly associated with the initial visual acuity(P<0.005),posterior extent of wound(P<0.001),length of wound(P<0.001),presence of hyphaema(P<0.001)and presence of vitreous prolapse(P<0.005).CONCLUSION:The most common causes of open globe injury are domestic accidents and occupational *** prognostic factors for final visual outcome in patients with open globe injury are initial visual acuity,posterior extent and length of wound,presence of hyphaema and presence of vitreous *** of the factors predicting

关键词： eye injuries occupational injuries vision disability visual impairment accidents intraocular foreign body

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Short-term toxic effects of chlorobenzenes on broadbean (Vicia faba) seedlings

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Science China(Life Sciences) 2005年第z1期48卷 33-39页

作者： LIU wan LI Peijun ZHOU Qixing SUN Tieheng tai Peidong XU Huaxia Key Laboratory of Terrestrial Ecological Process Institute of Applied EcologyChinese Academy of SciencesShenyang 110016China

The root growth,changes in superoxide dismutase(SOD,EC 1.***.1.1)activity,malonyldialdehyde(MDA)and total soluble protein level of broadbean(Vicia faba)seedlings were researched at different soil concentrations of chlorobenzene(CB),1,2,4-trichlorobenzene(TCB)and hexachlorobenzene(HCB).The results showed that root growth of seedlings was interrupted after 5d of 50-200μg·g^(-1)TCB *** a 3 d of recovery period,root growth was,however,restored to some extent although there was a delay in returning to the control *** total soluble protein content in seedlings increased with TCB concentration and duration of *** of TCB stress on SOD activity in seedlings displayed a significant dose-effect relationship for 1-5 d of 50-200μg·g^(-1)*** broadbean seedlings were placed in clean tap water for 3 d following exposure to 5 d of TCB stress to clear tap water for 3 d,SOD activity at 50μg·g^(-1)TCB recovered towards control level(P>0.05)while a significant increase in SOD activity was observed at 100 and 200μg·g^(-1)TCB compared to control(P<0.05).The experiments also revealed that a significant increase of MDA level in seedlings occurred after 3 and 5 d of 100 and 200μg·g^(-1)TCB treatment(P<0.05 and P<0.01),and there was a positive correlation between TCB concentration and MDA *** the above results showed that SOD activity and MDA level of broadbean seedlings might be proposed as the biomarkers for short-term TCB contamination in *** to TCB,the toxicity of 50-1000μg·g^(-1)CB or HCB in soil to broadbean seedlings was not observed after a 3 d exposure.

关键词： soil chlorobenzenes contamination broadbean SOD activity Biomarkers recovery

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Comparison of peripapillary retinal nerve fiber layer thickness between myopia severity groups and controls

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International Journal of Ophthalmology(English edition) 2018年第2期11卷 274-278页

作者： Evelyn Li Min tai Jiunn Loong Ling Eng Hui Gan Hussein Adil wan-Hiram wan-Hazabbah Department of Ophthalmology School of Medical SciencesHealth CampusUniversiti Sains MalaysiaKubang Kerian 16150KelantanMalaysia Hospital Universiti Sains Malaysia Kubang Kerian 16150KelantanMalaysia

AIM： To compare the peripapillary retinal nerve fiber layer（RNFL） thickness measured via optical coherence tomography（OCT） between different groups of myopia severity and controls. METHODS： This was a prospective cross-sectional study. All subjects underwent a full ophthalmic examination, refraction, visual field analysis and A-scan biometry. Myopic patients were classified as low myopia（LM） [spherical equivalent（SE） from greater than-0.5 D, up to-3.0 D], moderate myopia（MM; SE greater than-3.0 D, up to-6.0 D） and high myopia（HM; SE greater than-6.0 D）. The control group consisted of emmetropic（EM） patients（SE from ＋0.5 D to-0.5 D）. A Zeiss Cirrus HD-OCT machine was used to measure the peripapillary RNFL thickness of both eyes of each subject. The mean peripapillary RNFL thickness between groups was compared using both analysis of variance and analysis of ***： A total of 403 eyes of 403 subjects were included in this study. The mean age was 31.48±10.23 y. There were 180（44.7%） eyes with EM, 124（30.8%） with LM, 73（18.1%） with MM and 26（6.5%） with HM. All groups of myopia severity had a thinner average RNFL than the EM group, but after controlling for gender, age, and axial eye length, only the HM group differed significantly from the EM group（P=0.017）. Likewise, the superior, inferior and nasal RNFL was thinner in all myopia groups compared to controls, but after controlling for confounders, only the inferior quadrant RNFL was significantly thinner in the HM group, when compared to the EM group（P=0.017）. CONCLUSION： The average and inferior quadrant RNFL is thinner in highly myopic eyes compared to emmetropic eyes. Refractive status must be taken into consideration when interpreting the OCT of myopic patients, as RNFL thickness varies with the degree of myopia.

关键词： optical coherence tomography retinal nerve fiber layer myopia

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Mental retardation,seizures and language delay caused by new SETD1B mutations:Three case reports

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World Journal of Clinical Cases 2024年第2期12卷 383-391页

作者： Le Ding Li-wan Wei tai-Song Li Jing Chen Department of Neurology Children’s Hospital of Nanjing Medical UniversityNanjing 210000Jiangsu ProvinceChina Chigene(Beijing)Translational Medical Research Center Co.Ltd.Beijing 101111China

BACKGROUND The SETD1B gene is instrumental in human intelligence and nerve *** in the SETD1B gene have been linked in recent studies to neurodevelopmental disorders,seizures,and language *** SUMMARY This study aimed to analyze the clinical manifestations and treatment of three patients suffering from mental retardation,epilepsy,and language delay resulting from a new mutation in the SETD1B *** individuals with these symptoms were selected,and their clinical symptoms,gene test results,and treatment were *** article discusses the impact of the SETD1B gene mutation on patients and outlines the treatment *** the three patients(two females and one male,aged 8,4,and 1,respectively),all exhibited psychomotor retardation,attention deficit,and hyperactivity disorder,and two had *** treatment with sodium tripolyvalproate halted the seizures in the affected child,although mental development remained somewhat *** exome sequencing revealed new mutations in the SETD1B gene for all patients,specifically with c.5473C>T(***1825trp),c.4120C>T(***1374*,593),c.14_15insC(***5Hisfs*33).CONCLUSION Possessing the SETD1B gene mutation may cause mental retardation accompanied by seizures and language *** the exact mechanism is not fully understood,interventions such as drug therapy,rehabilitation training,and family support can assist patients in managing their symptoms and enhancing their quality of ***,genetic testing supplies healthcare providers with more precise diagnostic and therapeutic guidance,informs families about genetic disease risks,and contributes to understanding disease pathogenesis and drug research and development.

关键词： Neurodevelopmental disorder Seizure SETD1B gene Whole-exome sequencing New mutation Case report

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A FIASCO-Based Approach for Detection and Diagnosis of Puccinia graminis *** in China

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Journal of Integrative Agriculture 2014年第11期13卷 2438-2444页

作者： LIU tai-guo wanG Xi GAO Li LIU Bo CHEN wan-quan XIANG Wen-sheng State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences College of Life Science Northeast Agricultural University

Stem or black rust of wheat, caused by the fungus Puccinia graminis Pers. f. sp. tritici Eriks.＆E. Henn. （Pgt）, has historically caused severe losses to wheat （Triticum aestivum L.） production worldwide. In the Fujian and Guangdong provinces of China, six moderate-to-severe epidemics of wheat stem rust have occurred, which caused destructive losses of wheat between 1949 and 1966, although these were brought under control by integrated management. A rapid and reliable detection of the pathogen will contribute to the accurate forecast and seasonal control of this disease. The objective of this study was to develop a diagnostic molecular marker generated from simple sequence repeats （SSR） for the early rapid identiifcation of P. graminis. The genomic DNA of P. graminis, Puccinia striiformis, Puccinia triticina and seven other species was ampliifed by a pair of SSR primers generated by the FIASCO （fast isolation by AFLP sequences containing repeats） enrichment protocol. The primer set Pgtw （f）/Pgtw （r） generated a polymorphic pattern displaying a 330-bp DNA fragment speciifc for P. graminis whereas no DNA fragment was obtained from other non-target wheat fungal pathogens. The detection limit of the primer was 1 ng DNA in a 25-mL PCR reaction. The SSR markers of P. graminis can also be used to detect the presence of latent hyphae in Pgt-infected wheat leaves as early as 30 h post-inoculation. A rapid approach to distinguish P. graminis from similar pathogenic fungi would be anticipated in further study.

关键词： wheat stem rust Puccinia graminis f. sp. tritci microsatellite FIASCO PCR detection

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High-resolution ARPES endstation for in situ electronic structure investigations at SSRF

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Nuclear Science and Techniques 2021年第3期32卷 94-106页

作者： Yi-Chen yang Zheng-tai Liu Ji-Shan Liu Zhong-Hao Liu wan-Ling Liu Xiang-Le Lu Hong-Ping Mei Ang Li Mao Ye Shan Qiao Da-Wei Shen State Key Laboratory of Functional Materials for Informatics Shanghai Institute of Microsystem and Information TechnologyChinese Academy of SciencesShanghai 200050China University of Chinese Academy of Sciences Beijing 100049China

Angle-resolved photoemission spectroscopy(ARPES)is one of the most powerful experimental techniques in condensed matter *** ARPES,which uses photons with high flux and continuously tunable energy,has become particularly ***,an excellent synchrotron ARPES system must have features such as a small beam spot,super-high energy resolution,and a user-friendly operation interface.A synchrotron beamline and an endstation(BL03 U)were designed and constructed at the Shanghai Synchrotron Radiation *** beam spot size at the sample position is 7.5(V)μm×67(H)μm,and the fundamental photon range is 7-165 eV;the ARPES system enables photoemission with an energy resolution of 2.67 meV at21.2 *** addition,the ARPES system of this endstation is equipped with a six-axis cryogenic sample manipulator(the lowest temperature is 7 K)and is integrated with an oxide molecular beam epitaxy system and a scanning tunneling microscope,which can provide an advanced platform for in situ characterization of the fine electronic structure of condensed matter.

关键词： Synchrotron ARPES In situ VUV laser Quantum materials

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Preparation,Crystal Structure and Fungicidal Activity of N-(5-(benzofuranol-7-oxymethyl)-1,3,4-thiadiazol-2-yl)amide Compounds

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Chinese Journal of Structural Chemistry 2022年第3期41卷 211-217,I0013页

作者： wanG Chun-Nong ZENG tai-Ning LI Sheng-Nan LI wan LI Long-Fei CAO Feia yang Zi-Hui College of Pharmaceutical Sciences Key Laboratory of Analytical Science and Technology of Hebei ProvinceKey Laboratory of Medicinal Chemistry and Molecular Diagnosis of Ministry of EducationHebei UniversityBaoding 071002China Shan Dong Jinhuahai Biotechnology Co. Ltd Jinan 251400China

A variety of new N-(5-(benzofuranol-7-oxymethyl)-1,3,4-thiadiazol-2-yl)amide compounds(8a-i)were synthesized through four steps from benzofuranol as raw *** crystal structure of compound 8a(C17H21N3O3S,Mr=347.43)was measured by X-ray diffraction,which was classified as monoclinic system,Z=4,V=1742.72(8)Å3,Dc=1.324 Mg/m3,F(000)=736,S=1.03,μ=0.21 mm-1,space group P21 with a=9.9177(3),b=8.9519(2),c=19.8679(5)Å,the final R=0.035 and wR=0.105 for 3873 observed reflections(I>2σ(I)).The X-ray structure presented N(3)-H(3)···N(2)and C(6)-H(6)···O(3)intermolecular hydrogen bonds,which acted as an important role in stabilizing the crystal ***,preliminary biological assay on compound 8a showed good fungicidal activity in vivo,with the inhibition of 75%against Pseudoperonospora cu-bensis at 200 mg/L.

关键词： N-(5-(benzofuranol-7-oxymethyl)-1,3,4-thiadiazol-2-yl)amide,preparation,crystal structure,antifungal activity

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Shade adaptive response and yield analysis of different soybean genotypes in relay intercropping systems

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Journal of Integrative Agriculture 2017年第6期16卷 1331-1340页

作者： WU Yu-shan yang Feng GONG wan-zhuo Shoaib Ahmed FAN Yuan-fang WU Xiao-ling YONG tai-wen LIU Wei-guo SHU Kai LIU Jiang DU Jun-bo yang Wen-yu College of Agronomy Sichuan Agricultural University Chengdu 611130 P.R. China Sichuan Engineering Research Center for Crop Strip Intercropping System Chengdu 611130 P.R. China Key Laboratory of Crop Ecophysiology and Farming System in Southwest Ministry of Agriculture Chengdu 611130 P.R. China Characteristic Crops Research Institute Chongqing Academy of Agricultural Sciences Chongqing 402160 P.R. China

Soybean is one of the major oil seed crops,which is usually intercropped with other crops to increase soybean production area and ***,soybean is highly sensitive to *** is unclear if soybean morphology responds to shading（i.e.,shade tolerance or avoidance）and which features may be suitable as screening materials in relay strip ***,in this study,various agronomic characteristics of different soybean genotypes were analyzed under relay intercropping *** soybean materials used in this study exhibited genetic diversity,and the coefficient of variations of the agronomic parameters ranged from 13.84 to 72.08%during the shade period and from 6.44 to 52.49%during the maturity *** ratios of shading to full irradiance in stem mass fraction（SMF）were almost greater than 1,whereas opposite results were found in the *** with full irradiance,the average stem length（SL）,leaf area ratio（LAR）and specific leaf area（SLA）for the two years（2013 and 2014）increased by 0.78,0.47 and 0.65 under shady conditions,***,the stem diameter（SD）,total biomass（TB）,leaf area（LA）,number of nodes（NN）on the main stem,and number of branches（BN）all *** the shady period,the SL and SMF exhibited a significant negative correlation with yield,and the SD exhibited a significant positive correlation with *** correlation between the soybean yield and agronomic parameters during the mature period,except for SL,the first pod height（FPH）,100-seed weight（100-SW）,and reproductive growth period（RGP）,were significant（P〈0.01）,especially for seed weight per branch（SWB）,pods per plant（PP）,BN,and vegetative growth period（VGP）.These results provide an insight into screening the shade tolerance of soybean varieties and can be useful in targeted breeding programs of relay intercropped soybeans.

关键词： intercropping light morphological parameters shade avoidance soybean

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Population genetic structure of Chinese Puccinia triticina races based on multi-locus sequences

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Journal of Integrative Agriculture 2018年第8期17卷 1779-1789页

作者： LIU tai-guo GE Run-jing MA Yu-tong LIU Bo GAO Li CHEN wan-quan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant Protection Chinese Academy of Agricultural Sciences

Puccinia triticina, the causal agent of wheat leaf rust, is one of the most devastating rust fungi attacking wheat worldwide. Seventy-six isolates of the wheat leaf rust pathogen from Yunnan, Sichuan, Gansu and Henan provinces, China, were tested on wheat leaf rust differentials and the population structure was analyzed using four presumably neutral partial sequence markers such as elongation factor-1α(EF-1α), glyceraldehyde-3-phosphate dehydrogenase(GAPDH), β-tubulin(TUB) and the second largest RNA polymerase subunit(RPB2). The phenotypic diversity of Yunnan and Sichuan populations was higher than that of Gansu and Henan populations. The four populations were separated into two clusters based on the pathogenic data. A total of 12 single nucleotide polymorphisms(SNPs) and 32 haplotypes were identified among the four sequences. The 32 haplotypes were divided into two clusters in a neighbor-joining tree. Bayesian analyses also identified two clusters. Pairwise FST between populations in different regions were significantly different(P<0.05). Analysis of molecular variance(AMOVA) indicated that 68% of the total genetic variation was within populations.

关键词： population structure polymorphism virulence wheat leaf rust Puccinia triticina

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Jellyfish Green Fluorescent Protein(GFP) as a Reporter for Fusarium gramminearum Development on Wheat

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Journal of Integrative Agriculture 2014年第10期13卷 2177-2183页

作者： QI Jun-xian LIU tai-guo XU Ying CHEN Huai-gu GAO Li LIU Bo CHEN wan-quan State Key Laboratory for Biology of Plant Diseases and Insect Pests Institute of Plant ProtectionChinese Academy of Agricultural Sciences Institute of Plant Protection Jiangsu Academy of Agricultural Sciences

The plasmid pGPDGFP under the control ofpgpdA promotor was used together with vector pAN7-1 containing the hygromycin resistance cassette to co-transform protoplasts of HG1, Fusarium graminearum from Hubei Province, China. Twelve out of 14 hygromycin-resistant transformants showed green signal under the UV light and contained one or several copies ofgfp, as indicated by Southern analysis of genomic DNA digested with different restriction enzymes and hybridized to the gfp probe. A single gfp copy transformant （HG1C5） was selected for further evaluation of 80 Chinese wheat cultivars or advanced lines. The results showed different resistance type to F. graminearum were observed. GFP signals observed in the rachis and adjacent spikes of 70 Chinese wheat lines such as Chuanchongzu 104 indicated both type I （host resistance to the initial infection by the fungus） and type II （resistance to the spread of FHB symptoms within an infected spike） were not observed. While other 10 lines showed type II resistance to F. graminearum with GFP signals only in inoculated spikelets. Development of the mycelium can be intuitively observed and the resistance of wheat to F. graminearum can be identified at 7 days post inoculation （dpi） in this way. The results showed no differences were evaluated between the transformed HG1C5 and the non-transgene artificial inoculation by SAS paired chi-square test and McNernar＇s test （P=-0.0625）.

关键词： Fusarium head blight (FHB) green fluorescent protein (GFP) resistance evaluation

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