Short-term toxic effects of chlorobenzenes on broadbean (Vicia faba) seedlings

作     者：LIU Wan LI Peijun ZHOU Qixing SUN Tieheng TAI Peidong XU Huaxia 

作者机构：Key Laboratory of Terrestrial Ecological ProcessInstitute of Applied EcologyChinese Academy of SciencesShenyang 110016China 

出 版 物：《Science China(Life Sciences)》 (中国科学(生命科学英文版))

年 卷 期：2005年第48卷第z1期

页      面：33-39页

核心收录：

学科分类：07[理学] 08[工学] 

基　　金：This work was supported by the National Natural Science Foundation of China(Grant Nos.20377043,20225722,2004CB418506 and 20337010) the Ecological Experimental Station Foundation of the Chinese Academy of Sciences 

主　　题：soil chlorobenzenes contamination broadbean SOD activity Biomarkers recovery 

摘      要：The root growth,changes in superoxide dismutase(SOD,EC 1.***.1.1)activity,malonyldialdehyde(MDA)and total soluble protein level of broadbean(Vicia faba)seedlings were researched at different soil concentrations of chlorobenzene(CB),1,2,4-trichlorobenzene(TCB)and hexachlorobenzene(HCB).The results showed that root growth of seedlings was interrupted after 5d of 50-200μg·g^(-1)TCB *** a 3 d of recovery period,root growth was,however,restored to some extent although there was a delay in returning to the control *** total soluble protein content in seedlings increased with TCB concentration and duration of *** of TCB stress on SOD activity in seedlings displayed a significant dose-effect relationship for 1-5 d of 50-200μg·g^(-1)*** broadbean seedlings were placed in clean tap water for 3 d following exposure to 5 d of TCB stress to clear tap water for 3 d,SOD activity at 50μg·g^(-1)TCB recovered towards control level(P0.05)while a significant increase in SOD activity was observed at 100 and 200μg·g^(-1)TCB compared to control(P0.05).The experiments also revealed that a significant increase of MDA level in seedlings occurred after 3 and 5 d of 100 and 200μg·g^(-1)TCB treatment(P0.05 and P0.01),and there was a positive correlation between TCB concentration and MDA *** the above results showed that SOD activity and MDA level of broadbean seedlings might be proposed as the biomarkers for short-term TCB contamination in *** to TCB,the toxicity of 50-1000μg·g^(-1)CB or HCB in soil to broadbean seedlings was not observed after a 3 d exposure.