检索结果-南通市图书馆

黄芩苷通过抑制ROS介导的PI3K/Akt/FoxO3a信号通路诱导HepG2细胞铁死亡

在线全文

学校读者我要写书评

暂无评论

中国中药杂志 2024年第5期49卷 1327-1334页

作者：周季青黎华建曾煜豪陈画画梁炜瑜张进丁富平广州中医药大学基础医学院中西医结合基础研究中心广东广州510006 广州中医药大学护理学院广东广州510006

运用网络药理学和体外实验探究黄芩苷是否诱导HepG2细胞发生铁死亡并探讨潜在机制。体外培养HepG2细胞,CCK-8法检测细胞活力。TCGA数据库获取肝癌转录组测序数据,FerrDb V2数据库获取铁死亡基因数据。使用DEG2程辑包筛选差异表达基因,... 详细信息

运用网络药理学和体外实验探究黄芩苷是否诱导HepG2细胞发生铁死亡并探讨潜在机制。体外培养HepG2细胞,CCK-8法检测细胞活力。TCGA数据库获取肝癌转录组测序数据,FerrDb V2数据库获取铁死亡基因数据。使用DEG2程辑包筛选差异表达基因,并与铁死亡基因取交集,得到介导铁死亡调节肝癌进程的靶向基因。通过基因本体数据库(Gene Ontology,GO)与京都基因与基因组百科全书数据库(Kyoto Encyclopedia of Genes and Genomes,KEGG)以P0.5为标准,分析模块相关的分子功能及结构。DCFH-DA探针检测细胞活性氧(reactive oxygen species,ROS)水平变化。试剂盒检测细胞还原型谷胱甘肽(glutathione,GSH)、Fe^(2+)水平。实时荧光定量聚合酶链式反应(realtime quantitative PCR,RT-PCR)检测谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)、溶质载体家族7成员11(solute carrier family 7 member 11,SLC7A11)mRNA表达。蛋白质印迹法检测GPX4、SLC7A11、PI3K、p-PI3K、Akt、p-Akt、FoxO3a、p-FoxO3a蛋白表达。CCK-8检测结果显示,200μmol·L-1黄芩苷干预48 h,能显著抑制HepG2细胞活力。网络药理学结果显示,可通过PI3K/Akt信号通路调节肝癌中铁死亡的发生。细胞实验结果显示,黄芩苷可下调SLC7A11,降低GSH水平和GPX4表达,诱导HepG2细胞ROS累积,增加Fe^(2+)产生。铁死亡抑制剂(ferrostatin-1,Fer-1)可减少黄芩苷诱导的ROS累积,上调SLC7A11、GSH和GPX4表达,减弱PI3K、Akt、FoxO3a磷酸化。综上,黄芩苷可通过抑制ROS介导的PI3K/Akt/FoxO3a通路诱导HepG2细胞铁死亡。

关键词：肝细胞癌黄芩苷铁死亡 PI3K/Akt/FoxO3a信号通路

Eukaryotic initiation factor 5A2 and human digestive system neoplasms

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastrointestinal Oncology 2019年第6期11卷 449-458页

作者： Qing-Bin Meng Jing-Jing Peng Zi-wei Qu Xiao-Min Zhu Zhang Wen wei-Ming Kang Department of Gastrointestinal Surgery the First Hospital of Wuhan CityWuhan 430022Hubei ProvinceChina Department of Gastroenterology General Hospital of the Yangtze River ShippingWuhan 430015Hubei ProvinceChina 不详 Department of Hepato-Biliary-Pancreatic Surgery and Liver Transplantation the First Affiliated Hospital of Guangxi Medical UniversityNanning 530021Guangxi Zhuang Autonomous RegionChina Department of General Surgery Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China

Eukaryotic initiation factor 5A2(eIF5A2),as one of the two isoforms in the family,is reported to be a novel oncogenic protein that is involved in multiple aspects of many types of human *** or gene amplification of EIF5A2 has been demonstrated in many *** evidence shows that eIF5A2 initiates tumor formation,enhances cancer cell growth,increases cancer cell metastasis,and promotes treatment resistance through multiple means,including inducing epithelial–mesenchymal transition,cytoskeletal rearrangement,angiogenesis,and metabolic *** of eIF5A2 in cancer correlates with poor survival,advanced disease stage,as well as metastasis,suggesting that eIF5A2 function is crucial for tumor development and maintenance but not for normal tissue *** these studies suggest that eIF5A2 is a useful biomarker in the prediction of cancer prognosis and serves as an anticancer molecular *** review focuses on the expression,subcellular localization,post-translational modifications,and regulatory networks of eIF5A2,as well as its biochemical functions and evolving clinical applications in cancer,especially in human digestive system neoplasms.

关键词： Eukaryotic translation initiation factor 5A2 Hypusine modification Acetylation modification Drug resistance Cancer therapeutics

中医药干预非酒精性脂肪性肝病随机对照临床研究结局指标的现状分析

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中西医结合肝病杂志 2024年第2期34卷 136-139页

作者：姚伟伟刘可佳吉静赵欣宇程发峰赵静洁首都医科大学附属北京友谊医院中医科北京100050 北京中医药大学中医学院首都医科大学代谢相关脂肪性肝病中西医结合诊疗与研究中心首都医科大学附属北京友谊医院流行病学与循证医学研究室

目的:总结并评价中医药干预非酒精性脂肪性肝病(NAFLD)随机对照临床试验(RCTs)中的结局指标使用现状,为该领域临床研究的结局指标选择提供参考。方法:系统检索英文数据库Medline、EMbase、Cochrane Library,中文数据库中国知网(CNKI)、... 详细信息

目的:总结并评价中医药干预非酒精性脂肪性肝病(NAFLD)随机对照临床试验(RCTs)中的结局指标使用现状,为该领域临床研究的结局指标选择提供参考。方法:系统检索英文数据库Medline、EMbase、Cochrane Library,中文数据库中国知网(CNKI)、维普(VIP)和万方(Wanfang),时间为2018年1月1日至2022年12月31日发表的中医药治疗NAFLD的RCTs相关文献,采用定性分析方法,描述纳入研究的结局指标情况。结果:初步检索出相关文献2537篇,最终纳入78篇。78项临床研究的结局指标主要分为6类、15种,包括临床疗效评价类指标、肝脏无创检查指标、生化相关指标、人体测量学及生活质量相关指标、安全性指标和其他类。结论:中医药治疗NAFLD的RCTs结局指标的选择存在较多问题,针对复杂疾病的多维度评价应该借鉴国际核心指标集的创建方法,构建符合中医药治疗措施、疗效特色的核心结局指标集。

关键词：非酒精性脂肪性肝病结局指标中医药随机对照临床研究

Unexpected guests in the tumor microenvironment:microbiome in cancer

在线全文

学校读者我要写书评

暂无评论

Protein & Cell 2021年第5期12卷 426-435页

作者： Abigail Wong-Rolle Haohan Karen wei Chen Zhao Chengcheng Jin Thoracic and Gastrointestinal Malignancies Branch National Cancer InstituteNational Institutes of Health4 Memorial DriveBethesdaMD 20892USA Department of Cancer Biology Perelman School of MedicineUniversity of Pennsylvania421 Curie Blvd.PhiladelphiaPA 19104USA

Although intestinal microbiome have been established as an important biomarker and regulator of cancer development and therapeutic response, less is known about the role of microbiome at other body sites in cancer. Emerging evidence has revealed that the local microbiota make up an important part of the tumor microenvironment across many types of cancer, especially in cancers arising from mucosal sites, including the lung, skin and gastrointestinal tract. The populations of bacteria that reside specifically within tumors have been found to be tumor-type specific, and mechanistic studies have demonstrated that tumor-associated microbiota may directly regulate cancer initiation, progression and responses to chemo- or immuno-therapies. This review aims to provide a comprehensive review of the important literature on the microbiota in the cancerous tissue, and their function and mechanism of action in cancer development and treatment.

关键词： microbiome tumor lung cancer immune system tumor-associated microbiota cancer immunotherapy

Efficacy of immunosuppression monotherapy after liver transplantation:A meta-analysis

在线全文

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2014年第34期20卷 12330-12340页

作者： Xiang Lan Meng-Gang Liu Hong-Xu Chen Hong-Ming Liu wei Zeng Dong wei Ping Chen Department of Hepatobiliary Surgery Daping Hospitalthe Third Military Medical University

AIM: To assess the advantages and disadvantages of immunosuppression monotherapy after transplantation and the impact of monotherapy on hepatitis C virus (HCV) recurrence.

关键词： Liver transplantation Immunosuppression monotherapy Cytomegalovirus Diabetes Meta-analysis

Efficacy of Guhong injection versus Butylphthalide injection for mild ischemic stroke: A multicenter controlled study

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of clinical Cases 2022年第21期10卷 7265-7274页

作者： wei-wei Zhang Jiang Xin Guang-Yu Zhang Qi-Jin Zhai Hua-Min Zhang Cheng-Si Wu Department of Neurology The Seventh Medical Center of PLA General HospitalBeijing 100039China Department of Neurology Liaoning Provincial People’s HospitalShenyang Liaoning Provincial People's HospitalShenyang 110016Liaoning ProvinceChina Department of Neurology Cangzhou City People’s HospitalCangzhou 061000Hebei ProvinceChina Department of Neurology Huai’an Second People’s HospitalHuai’an 223002Jiangsu ProvinceChina Department of Neurology Ganyu People’s Hospital of LianyungangGanyu 222100Jiangsu ProvinceChina Department of Neurology The First Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina

BACKGROUND Most studies on Guhong injection have involved a single center with a small sample size,and the level of clinical evidence is *** To assess the safety and efficacy of Guhong injection for mild ischemic stroke(IS).METHODS A total of 399 IS patients treated at six hospitals from August 2018 to August 2019 were retrospectively *** patients were given Guhong injection(experimental group)or Butylphthalide injection(control group).Changes in National institutes of Health Stroke Scale(NIHSS)and modified Rankin Scale(mRS)scores were observed before treatment and at 1,2,and 3 wk after treatment in each *** efficacy and safety of Guhong injection for IS were *** medications taken by the patients were confounding factors for efficacy *** factors were controlled by propensity score matching,and the results were further analyzed based on the *** The marked response rates at three follow-up visits were 64.64%,74.7%,and 66.7%in the experimental group,and 48.26%,45.4%,and 22.2%in the control *** marked response rates increased significantly in the experimental group compared with the control group(P0.05)and 64.0%and 47.7%in the control group(P<0.05)at the first

关键词： Guhong injection Ischemic stroke Propensity score matching National institutes of Health Stroke Scale

Effects of early enteral nutrition on immune function of severe acute pancreatitis patients

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of Gastroenterology 2013年第6期19卷 917-922页

作者： Jia-Kui Sun Xin-wei Mu wei-Qin Li Zhi-Hui Tong Jing Li Shu-Yun Zheng Department of Intensive Care Unit Nanjing First HospitalNanjing Medical University Department of General Surgery Jinling HospitalNanjing University School of Medicine

AIM:To investigate the effects of early enteral nutrition (EEN) on the immune function and clinical outcome of patients with severe acute pancreatitis (SAP).METHODS:Patients were randomly allocated to receive EEN or delayed enteral nutrition (DEN).Enteral nutrition was started within 48 h after admission in EEN group,whereas from the 8 th day in DEN *** the immunologic parameters and C-reactive protein (CRP) levels were collected on days 1,3,7 and 14 after *** clinical outcome variables were also ***:Sixty SAP patients were enrolled to this *** CD4+ T-lymphocyte percentage,CD4+/CD8+ ratio,and the CRP levels in EEN group became significantly lower than in DEN group from the 7 th day after *** contrast,the immunoglobulin G(IgG) levels and human leukocyte antigen-DR expression in EEN group became significantly higher than in DEN group from the 7 th day after *** difference of CD8+ T-lymphocyte percentage,IgM and IgA levels was found between the two *** incidences of multiple organ dysfunction syndrome,systemic inflammatory response syndrome,and pancreatic infection as well as the duration of intensive care unit stay were significantly lower in EEN group than in DEN ***,there was no difference of hospital mortality between the two ***:EEN moderates the excessive immune response during the early stage of SAP without leading to subsequent *** can improve the clinical outcome,but not decrease the hospital mortality of SAP patients.

关键词： Early enteral nutrition Immune Severe acute pancreatitis

Endoscopic diagnosis and treatment of an appendiceal mucocele:A case report

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

World Journal of clinical Cases 2021年第16期9卷 3936-3942页

作者： Ting-Ting Wang Jia-Jun He Ping-Hong Zhou wei-wei Chen Chao-Wu Chen Jun Liu Department of Gastroenterology The First Clinical Medical CollegeDalian Medical UniversityDalian 116044Liaoning ProvinceChina Department of General Surgery Zhongshan HospitalFudan UniversityShanghai 200032China Endoscopy Center Department of GastroenterologyClinical Medical CollegeYangzhou UniversityYangzhou 225009Jiangsu ProvinceChina

BACKGROUND Appendiceal mucocele is a rare disease that causes obstructive dilatation of the appendix due to the intraluminal accumulation of *** report a case of endoscopic diagnosis and treatment of an appendiceal *** SUMMARY A 47-year-old man presented with a protrusion around the orifice of the appendix discovered by colonoscopy *** was admitted to our hospital for a routine checkup without any *** computed tomography showed a cystic mass approximately 3 cm in diameter with fat *** preoperative diagnosis was non-neoplastic appendiceal mucocele,and endoscopic treatment was *** endoscopic findings and pathological results supported our preoperative *** endoscopic treatment of appendiceal mucocele was feasible and effective,which was confirmed by repeated endoscopy and post-operative computed tomography after 7 *** Endoscopic therapy provides a new method for the treatment of appendiceal mucocele.

关键词： Appendiceal mucocele Endoscopy Colonoscopy Diagnosis Treatment Case report

腹腔镜同期双侧肾上腺切除术治疗甲状腺髓样癌致异位ACTH综合征1例报告并文献复习

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

中华泌尿外科杂志 2024年第4期45卷 299-305页

作者：沃奇军赵瑜杨伟段婷寿佳沣杨云开祁小龙张大宏浙江省人民医院、杭州医学院附属人民医院泌尿外科、浙江省内分泌腺体疾病诊治研究重点实验室杭州310014 浙江省人民医院、杭州医学院附属人民医院老年医学中心内分泌科杭州310014 浙江省人民医院、杭州医学院附属人民医院检验中心杭州310014 浙江省人民医院、杭州医学院附属人民医院病理科杭州310014

目的按探讨腹腔镜同期双侧肾上腺切除术(BA)治疗甲状腺髓样癌(MTC)致异位促肾上腺皮质激素综合征(EAS)的安全性和疗效。方法患者男,56岁,2023年10月5日因MTC术后15个月,全身乏力7个月入院。既往因MTC行两次甲状腺开放手术。病理诊断:甲... 详细信息

目的按探讨腹腔镜同期双侧肾上腺切除术(BA)治疗甲状腺髓样癌(MTC)致异位促肾上腺皮质激素综合征(EAS)的安全性和疗效。方法患者男,56岁,2023年10月5日因MTC术后15个月,全身乏力7个月入院。既往因MTC行两次甲状腺开放手术。病理诊断:甲状腺髓样癌合并上纵隔、纵隔等处淋巴结转移,CRH(-)、ACTH(胞质弱+)。患者术后出现糖尿病、高血压、低钾血症。入院查体:血压200/95mmHg(1mmHg=0.133kPa),体质量61.5kg,身高160cm,体质量指数24.02kg/cm^(2),腰围83cm,多血质外貌,皮肤变薄。实验室检查:血钾2.71mmol/L,血钙1.47mmol/L,甲状旁腺素6.0pg/ml,空腹血糖10.51mmol/L,糖化血红蛋白8.2%,血降钙素>2000pg/ml,癌胚抗原70.8μg/L。内分泌激素检查:8:00、16:00、24:00时血浆ACTH分别为189.0、125.0、65.0pg/ml;8:00、16:20、24:00时血清皮质醇分别为429.30、408.14、446.61μg/L;24h尿游离皮质醇1200μg/24h;1mg过夜地塞米松抑制试验后8:00时ACTH183.0pg/ml、皮质醇538.27μg/L;非卧位2h醛固酮8.2pg/ml;血、尿儿茶酚胺和甲状腺功能均未见明显异常。^(18)F-FDG-PET/CT检查示颈部等多发淋巴结转移。腹部CT检查示双侧肾上腺增生。垂体增强MR检查示垂体变薄。肺CT、痰培养检查示双肺散在多发感染。经全院多学科讨论,诊断为EAS、MTC术后转移、糖尿病、高血压病、低钾血症、肺部感染、轻度贫血、肝功能不全、甲状旁腺功能减退症、低钙血症。全麻下行经腹腔入路腹腔镜BA。术中先切除左侧肾上腺,翻转体位,再切除右侧肾上腺。结果本例手术顺利。手术时间约60min。术中出血量约20ml。围术期未发生并发症。病理检查:(双侧)肾上腺皮质结节性增生;(双侧)肾上腺髓质增生,ACTH(-)。术后随访3个月,血降钙素仍>2000pg/ml。术后1周,以及1、3个月血ACTH分别为183.0、220.0、731.0pg/ml。但高血压、糖尿病、低钾血症等均迅速缓解。术后1个月血压100/80mmHg,空腹血糖4.4mmol/L,血钾3.87mmol/L;肺部感染好转。未发生肾上腺危像。糖皮质激素替代治疗方案:氢化可的松晨起20mg、午后10mg。甲状腺激素替代治疗方案:左甲状腺素100μg每日1次。基因检测示Ret基因杂合突变,患者应用Ret抑制剂临床试验性治疗。结论对于无法根治的MTC转移病灶导致的EAS,采取腹腔镜同期BA是安全、有效的,可纠正高血压、糖尿病和低钾血症,增加了MTC的治疗机会。

关键词：异位促肾上腺皮质激素综合征甲状腺髓样癌库欣综合征双侧肾上腺切除术