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Construction and characterization of calreticulin-HBsAg fusion gene recombinant adenovirus expression vector

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World Journal of Gastroenterology 2010年第24期16卷 3078-3082页

作者： Ma, Chun-Ling wang, gui-bin Gu, Run-Guo wang, Fang Shandong Med Coll Immunol Lab Linyi 276002 Shandong Peoples R China Renmin Hosp Linyi City Imaging Div Linyi 276003 Shandong Peoples R China Nanjing Med Univ Affiliated Hosp 1 Dept Lab Med Nanjing 210029 Jiangsu Prov Peoples R China

AIM: To generate recombinant adenoviral vector con-taining calreticulin (CRT)-hepatitis B surface antigen (HBsAg) fusion gene for developing a safe, effective and HBsAg-specific therapeutic ***: CRT and HBsAg gene were fused using polymerase chain reaction (PCR), endonuclease diges-tion and ligation methods. The fusion gene was cloned into pENTR/D-TOPO transfer vector after the base pairs of DNA (CACC) sequence was added to the 5′ end. Adenoviral expression vector containing CRT-HBsAg fusion gene was constructed by homologous recombinan-tion. The human embryo kidney (HEK) 293A cells were transfected with linearized DNA plasmid of the recombi-nant adenoviral vector to package and amplify recombi-nant adenovirus. The recombinant adenovirus titer was characterized using the end-dilution assay. The expres-sion of the CRT/HBsAg fusion protein in Ad-CRT/HBsAg infected 293A cells was detected by Western ***: The CRT-HBsAg fusion gene was char-acterized by PCR and sequencing and its length and sequence were confirmed to be accurate. The CRT-HB-sAg fusion gene recombinant pENTR/D-TOPO transfer vector was constructed. The recombinant adenoviral vector, Ad-CRT/HBsAg, was generated successfully. The titer of Ad-CRT/HBsAg was characterized as 3.9 × 1011 pfu/mL. The CRT-HBsAg fusion protein was ex-pressed by HEK 293A cells correctly. CONCLUSION: CRT/HBsAg fusion gene recombinant replication-defective adenovirus expression vector is constructed successfully and this study has provided an experimental basis for further studies of Hepatitis B vi-rus gene therapy.

关键词： Calreticulin Hepatitis B virus Hepatitis B surface antigen Adenovirus expression vector Fusion protein Therapeutic vaccine

Synthesis and Properties of Poly(aryl ether ketone) Copolymers Containing 2,7-Naphthalene Moieties

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Chemical Research in Chinese Universities 2006年第1期22卷 114-117页

作者： LIU Si-jie NIU Ya-ming ZHU Xiao-liang wang gui-bin JIANG Zhen-hua Alan G. MacDiarmid Institute College of Chemistry Jilin University Changchun 130012 P.R. China

Poly（ aryl ether ketone） copolymers containing 2,7-naphthylene and hydroquinone（HQ） moieties with various compositions were prepared by the reaction of 4,4＇-difluorobenzophenone（DFB） 2,7-naphthylene and hydroquinone in the presence of sodium carbonate and potassium carbonate （ 19： 1, mass ratio） in diphenyl sulfone （DPS）. The thermal analysis results of the copolymers show that the glass transition temperatures（Tgs） increase, while the melting temperatures（Tra） and tile 5.0% weight loss temperatures （ Td ） decrease with the increase of the 2,7-naphthalene moieties. For the copolymers synthesized at a molar fraction of 2,7-naphthalene in the difluoro monomers being over 0.30, no peaks corresponding to the cold crystallization temperature and the melting temperature could be detected, indicating that these copolymers are almost amorphous. The crystal structures of the copolymers with a molar fraction of 2,7-naphthalene being less than 0. 30 are rhombic.

关键词： Poly( aryl ether ketone) Naphthalene derivatives Friedel-crafts acylation

Integrative analysis of the metabolome and transcriptome reveals seed germination mechanism in Punica granatum L.

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Journal of Integrative Agriculture 2021年第1期20卷 132-146页

作者： FU Fang-fang PENG Ying-shu wang gui-bin Yousry A.EL-KASSABY CAO Fu-liang Co-Innovation Center for Sustainable Forestry in Southern China Nanjing Forestry UniversityNanjing 210037P.R.China Department of Forest and Conservation Sciences Faculty of ForestryThe University of British ColumbiaVancouverB.C.V6T 1Z4Canada

We conducted an integrative system biology of metabolome and transcriptome profile analyses during pomegranate(Punica granatum L.) seed germination and utilized a weighted gene co-expression network analysis(WGCNA) to describe the functionality and complexity of the physiological and morphogenetic processes as well as gene expression and metabolic differences during seed germination stages. In total, 489 metabolites were detected, including 40 differentially accumulated metabolites. The transcriptomic analysis showed the expression of 6 984 genes changed significantly throughout the whole germination process. Using WGCNA, we identified modules related to the various seed germination stages and hub genes. In the initial imbibition stage(stage 1), the pivotal genes involved in RNA transduction and the glycolytic pathway were most active, while in the sprouting stage(stage 4), the pivotal genes were involved in multiple metabolic pathways. In terms of secondary metabolic pathways, we found flavonoid 4-reductase genes of anthocyanin biosynthesis pathway are most significantly affected during pomegranate seed germination, while the flavonol synthase gene was mainly involved in the regulation of isoflavonoid biosynthesis.

关键词： seed germination stages weighted gene co-expression network analysis(WGCNA) metabolome transcriptome flavonoid pathway

Crystallization Kinetics and Melting Behavior of PA1010/Ether-based TPU Blends

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Chemical Research in Chinese Universities 2007年第3期23卷 370-373页

作者： ZHANG Shu-ling ZHAO Yan SUN Xiao-bo JIANG Zhen-hua WU Zhong-wen wang gui-bin Alan G. MacDiarmid Lab. College of Chemistry Jilin University Changchun 130012 P. R. China

Polyamide 1010 （PA1010）/thermoplastie poly （ether urethane） elastomer （ether-based TPU） blends were prepared via melt extrusion. The crystallization kinetics and melting behavior of PA1010/ether-based TPU blends were systematically investigated using differential scanning calorimetry. The crystallization kinetics results show that the addition of ether-based TPU hinders the crystallization of PA1010, and the hindrance effect increases with the increase of the concentration of ether-based TPU. Both pure PA1010 and PA1010/ether-based TPU blends exhibit double melting peaks in the process of nonisothermal crystallization. The double melting peaks change differently with the variation of cooling rate and blend composition. The cooling rate only influences the lower melting peak; however, the blend composition influences not only the lower melting peak but also the higher melting peak. The reason for the phenomenon must be the interaction between the two compositions.

关键词： Kinetics Melting point Nylon TPU Differential scanning calorimetry(DSC)

Synthesis of Poly(aryl ether ketone) Copolymers Containing Adamantyl-substituted Naphthalene Rings

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Chemical Research in Chinese Universities 2009年第2期25卷 261-264页

作者： ZHU Xiao-liang ZHANG Shu-ling REN Dian-fu GUAN Shao-wei wang gui-bin JIANG Zhen-hua College of Chemistry Jilin University Changchun 130012 P R. China

High performance polymers have received considerable attention over the past decade owing to their increased demands as replacements for metals or ceramics in automotive, aerospace, and microelectronic industries. Poly（aryl ether ketone）s（PAEKs） are a class of important high-performance aromatic polymers with excellent mechanical properties, good solvent resistance, size-accuracy, electrical characteristics, and superior thermal stability. However,

关键词： Adamantyl group Naphthalene Poly(aryl ether ketone) Synthesis

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模型指导的多维GPU软件低功耗优化方法

计算机学报 2012年第5期35卷 979-989页

作者：王桂彬国防科学技术大学计算机学院并行与分布处理国家重点实验室长沙410073

作为众核体系结构的典型代表,GPU(Graphics Processing Units)芯片集成了大量并行处理核心,其功耗开销也在随之增大,逐渐成为计算机系统中功耗开销最大的组成部分之一,而软件低功耗优化技术是降低芯片功耗的有效方法.文中提出了一种模... 详细信息

作为众核体系结构的典型代表,GPU(Graphics Processing Units)芯片集成了大量并行处理核心,其功耗开销也在随之增大,逐渐成为计算机系统中功耗开销最大的组成部分之一,而软件低功耗优化技术是降低芯片功耗的有效方法.文中提出了一种模型指导的多维低功耗优化技术,通过结合动态电压/频率调节和动态核心关闭技术,在不影响性能的情况下降低GPU功耗.首先,针对GPU多线程执行模型的特点,建立了访存受限程序的功耗优化模型;然后,基于该模型,分别分析了动态电压/频率调节和动态核心关闭技术对程序执行时间和能量消耗的影响,进而将功耗优化问题归纳为一般整数规划问题;最后,通过对9个典型GPU程序的评测以及与已有方法的对比分析,验证了该文提出的低功耗优化技术可以在不影响性能的情况下有效降低芯片功耗.

关键词：低功耗优化 GPU 动态核心关闭动态电压/频率调节

Mechanism Research on Standardized Development of Rural Private Finance

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Asian Agricultural Research 2010年第8期2卷 27-30页

作者： ZOU Xin-yang wang gui-bin Department of Economic Management Southwest UniversityChongqing 400716China English Department Southwest UniversityChongqing 400716China

In generalizing the researching conditions of researchers on private finance,the paper introduces the connoted meaning of rural private finance broadly and *** paper states the forms of rural private finance(including private loaning,private bank,rural private collecting,financing organizations,cooperatives,NGO,small loaning organizations and so on),the relations between rural private finance and rural economic relations,pointing out that it is the combination of the strong and the weak,which may generate benefits with the operation of marketing *** paper analyzes the historical causes,supervision causes and cultural causes of rural private finance,discussing mechanisms of standardized development of rural private finance:firstly,standardize the organization management mechanisms,including scaled controlling mechanisms and bank management mechanisms;secondly,complete finance supervision mechanisms;thirdly,moderate government intervention,including affording liberal policy environment and reducing the improper intervention;fourthly,upgrading qualities mechanisms,including cultivating the rural credit culture and improving the quality of regulatory personnel.

关键词： Rural private finance Rural economy Financial supervision Standardized development China

基于通信感知任务划分的异构系统低功耗优化方法

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小型微型计算机系统 2011年第12期32卷 2504-2510页

作者：王桂彬国防科学技术大学计算机学院并行与分布处理国家重点实验室长沙410073

针对由通用微处理器和专用加速部件构成的异构并行系统,提出结合通信感知的并行任务划分和动态电压频率调节技术的异构系统能耗优化方法,该方法旨在将并行任务图划分并映射在异构处理单元,在满足性能约束的条件下最小化系统能耗.在目前... 详细信息

针对由通用微处理器和专用加速部件构成的异构并行系统,提出结合通信感知的并行任务划分和动态电压频率调节技术的异构系统能耗优化方法,该方法旨在将并行任务图划分并映射在异构处理单元,在满足性能约束的条件下最小化系统能耗.在目前典型异构并行系统中,主处理器与加速部件大都通过系统总线连接,必然引入不可忽略的通信开销,因此通信感知的任务划分技术是该问题的关键.提出了基于整数线性规划的静态最优能耗优化方法和基于遗传算法的动态能耗优化方法.并通过一个典型科学计算应用验证了本文方法的有效性.

关键词：低功耗优化异构并行系统任务划分动态电压频率调节

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一种面向异构并行系统的最大功耗管理方法

软件学报 2013年第10期24卷 2460-2472页

作者：王桂彬杜静唐滔国防科学技术大学计算机学院湖南长沙410073 电子信息系统复杂电磁环境效应国家重点实验室河南洛阳471000

高功耗已成为制约高性能计算机发展的重要问题之一.近年来,大量研究关注于如何在满足系统功耗约束的条件下优化系统执行性能.然而,已有方法大都针对同构系统,未考虑异构处理器之间的功耗或速度差异,难以高效应用于基于加速器的异构系统... 详细信息

高功耗已成为制约高性能计算机发展的重要问题之一.近年来,大量研究关注于如何在满足系统功耗约束的条件下优化系统执行性能.然而,已有方法大都针对同构系统,未考虑异构处理器之间的功耗或速度差异,难以高效应用于基于加速器的异构系统.对当前异构并行系统执行模型进行了抽象,并提出了融合两级功耗控制机制的系统功耗管理框架,自顶向下依次为系统级功耗控制器和异构处理引擎功耗控制器.在异构处理引擎功耗控制中,针对类OpenMP并行循环,首先分析了异构多处理器在满足功耗约束条件下达到性能最优的条件.基于该结果,给出了功耗受限的并行循环划分算法,该方法通过协调并行循环调度和动态电压频率调节技术以优化异构并行处理.在系统级功耗控制中,建立了异构处理引擎效能评估方法,以此作为功耗划分的依据,在兼顾并发应用公平性的同时,提高系统整体执行效能.最后,基于典型CPU-GPU异构系统验证了方法的有效性.

关键词：异构并行系统最大功耗管理任务调度动态电压频率调节