检索结果-南通市图书馆

Journal of Zhejiang University-Science B(Biomedicine & Biotechnology) 2011年第3期12卷 193-200页

作者： Shu-fang wang Hai-yan fang Hai-bin QU Pharmaceutical Informatics Institute Zhejiang University Hangzhou 310058 China

Central composite design(CCD),together with multiple linear regression,was successfully used to optimize the electrophoretic buffer system of micellar electrokinetic capillary chromatography(MEKC) for the determination of albiflorin,paeoniflorin,liquiritin,and glycyrrhizic acid in the traditional Chinese medicine(TCM) prescription,yangwei *** of sodium deoxycholate(SDC) and borate,and proportions of ammonia,acetonitrile,and methanol were *** total resolutions of peaks between the analytes and their adjacent peaks in real samples were integrated into the evaluation index of separation *** optimum electrophoretic buffer contained 80 mmol/L SDC,20 mmol/L borate,5%(v/v) methanol,0.5%(v/v) ammonia,and 5%(v/v) *** correlation coefficients(R 2 ) between the peak areas and the corresponding concentrations of analytes were greater than *** limits of detection(LODs) (S/N=3) of the analytes were 0.97-4.00μg/*** results indicate the superiority of CCD in optimizing the separation conditions of complex samples such as TCM prescriptions.

关键词： Micellar electrokinetic capillary chromatography Traditional Chinese medicine Central composite design yangwei granule

Synthesis of 2,4-Diarylcyclooctenopyridines with One-pot Four-component Reactions

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2009年第3期25卷 338-342页

作者： wang Qi-fang yan Chao-guo College of Chemistry ＆ Chemical Engineering Yangzhou University Yangzhou 225002 P. R. China

An efficient one-pot four-component reaction has been developed for the synthesis of 2,4-diarylcyclooctenopyridines, in moderate yields. This route is an effective modified two-step synthesis of Krohnke pyridine and involves a four-component tandem reaction of pyridinium bromide with aromatic aldehydes and cyclooctanone in a system of NHaOAc/HOAc under microwave irradiation.

关键词： Pyridine Cycloalkenopyridine Multicomponent reaction Microwave irradiation

Gene engineering in swine for agriculture

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2017年第12期16卷 2792-2804页

作者： wang yan-fang HUANG Jiao-jiao ZHAO Jian-guo Institute of Animal Science Chinese Academy of Agricultural Sciences Beijing 100193 P.R. China Institute of Zoology Chinese Academy of Sciences Beijing 100101 P.R.China University of Chinese Academy of Sciences Beijing 100049 P.R. China

Domestic pigs are the second most important source of meat world-wide, and the genetic improvement of economic traits, such as meat production, growth, and disease resistance, is a critical point for efficient production in pigs. Through conventional breeding and selection programs in pigs, which are painstakingly slow processes, some economic traits, such as growth and backfat, have been greatly improved over the past several decades. However, the improvement of many polygenetic traits is still very slow and challenging to be improved by conventional breeding strategies. The development of reproductive knowledge and a variety of techniques, including foreign gene transfer strategies, somatic cell nuclear transfer（SCNT） and particularly, recently developed nuclease-mediated genome editing tools, has provided efficient ways to produce genetically modified（GM） pigs for the dramatic improvement of economic traits. In this review, we briefly discuss the progress of genomic markers used in pig breeding program, trace the history of genetic engineering, mainly focusing on the progress of recently developed genome editing tools, and summarize the GM pigs which have been generated to aim at the agricultural purposes. We also discuss the specific challenges facing application of gene engineering in pig breeding, and future prospects.

关键词： gene engineering genome editing pig agricultural application

Efficient Synthesis of Dicycloalkenopyridines:One-pot Multicomponent Condensation of Aldehydes with Cyclic Ketones

在线全文

学校读者我要写书评

暂无评论

Chemical Research in Chinese Universities 2009年第5期25卷 657-661页

作者： CAI Xi-mei wang Qi-fang yan Chao-guo College of Chemistry ＆ Chemical Engineering Yangzhou University Yangzhou 225002 P. R. China

Under microwave irradiation, the one-pot multicomponent condensation reaction of three moles of aromatic aldehydes with two moles of cyclic ketones having free a,a＇-methylene positions such as cyclopentanone or cyclohexanone in the presence of ammonium acetate and acetic acid afforded dicycloalkenopyridines with two a-arylidene groups in high yields. Under the similar reaction condition, the reaction of aromatic aldehydes with 1-tetralone having only one a-methylene position alternatively resulted in 10-aryl-2,3：5,6-dibenzoacridines.

关键词： Pyridine Acridine Microwave irradiation Aromatic aldehyde Cyclic ketone Aldol condensation

Texture of Hot Rolled Strip for Fe-3Si Steel Produced by Thin Slab Casting and Rolling

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2010年第12期17卷 46-53页

作者： LI Chang-sheng yanG Hua wang yan-fang YU Yong-mei State Key Laboratory of Rolling and Automation Northeastern University Shenyang 110004 Liaoning China Shougang Qian'an Iron and Steel Corporation Qian'an 064404 Hebei China

Fe-3Si steel strip was produced by thin slab casting and rolling （TSCR） process in the laboratory. The microstructure and texture of hot rolled strip by different total reduction and rolling schedules were observed through EBSD technique and X-ray diffraction method. The rule of texture density for the a,ε and γ fibers was analyzed. When the total reduction was increased from 82.9% to 97.1%, the gradient of microstrueture and texture for A steel surface layer and center layer was found, {001}〈110〉 texture had higher intensity, and {001}〈110〉texture was changed to { 110 } 〈 001 〉 and { 112 } 〈 111 〉. The texture distribution with different reduction schedules for B steel was basically similar, but the Goss texture orientation density and volume fraction for B steel were higher, the hot rolling reduction schedule of C1 was effective for the formation of Goss texture. The research work was useful theoretical basis and reference data to develop electrical steel by thin slab continuous casting and direct rolling technology.

关键词： silicon steel texture hot rolling thin slab continuous casting direct rolling

Texture of Cold Rolled Strip of Fe-3Si Steel Produced by Thin Slab Casting and Rolling

在线全文

学校读者我要写书评

暂无评论

Journal of Iron and Steel Research International 2011年第3期18卷 40-46页

作者： LI Chang-sheng yanG Hua wang yan-fang YU Yong-mei State Key Laboratory of Rolling and Automation Northeastern UniversityShenyang 110004LiaoningChina Shougang Qian'an Iron and Steel Corporation Qian'an 064404HebeiChina

The hot rolled strip of Fe-3Si steel was experimentally produced by thin slab casting and rolling（TSCR） process in the *** texture evolution rule was investigated during hot rolling and cold *** texture distribution of cold rolled strips with four kinds of cold rolling reduction schedules was observed through X-ray diffraction method,and the orientation density variation of 1/16 layer,1/8 layer,1/4 layer,and 1/2 layer in thickness was *** cold rolled texture of steel A with four kinds of cold rolling reduction schedules was mainly composed of α and γ *** rolling reduction ratio of 70% was more beneficial for obtaining more γ *** γ fiber texture orientation density of {111}01-1 and {111}1-1-2 was the largest on the 1/8 layer and 1/4 layer and the least on the 1/16 layer and 1/2 layer for steel *** texture orientation density of {001}-{223}11-0 in the α fibers for steel C was increased,but the texture orientation density of {332}-{110}11-0 was low.

关键词： silicon steel texture hot rolling cold rolling thin slab continuous casting direct rolling

Efficacy and Mechanism of Buxue Yimu Pillson Gynecological Anemia:A Combination of Clinical and Network Pharmacology Study

在线全文

学校读者我要写书评

暂无评论

Chinese Journal of Integrative Medicine 2022年第12期28卷 1072-1080页

作者： wang yan-fang DENG yan ZHANG Su-ying LIU Dong LUO Bin wang Xue DENG Miao MA Rui-lin SUN Ai-jun Department of Obstetrics and Gynecology Peking Union Medical College HospitalChinese Academy of Medical Sciences and Peking Union Medical CollegeBeijing 100730China Department of Obstetrics and Gynecology the Second Affiliated Hospital of Hunan University of Chinese MedicineChangsha 410001China West China Second Hospital Key Laboratory of Birth Defects and Related Diseases of Women and ChildrenSichuan UniversityMinistry of EducationSichuan UniversityChengdu 610041China Department of Obstetrics and Gynecology Hangzhou Women's Hospital(Hangzhou Maternity and Child Health Care Hospital)Hangzhou 310008China

Objective:Tocompare the clinical efficacyand safety of oral administration of BuxueYimuPills(BYP),ferrous sulfate(FS),and the combination of BYP and FS on gynecological anemia,and investigate the mechanisms using network ***:A randomized,controlled,multi-center clinical trial was *** 150 patients with hemoglobin of 70-110 g/L due to gynecological conditions were recruited and randomized(using the block randomization method)into Buxue Yimu Pills group(24 g/d),oral iron group(FS Tablets,0.9 g/d),and combined treatment group(BYP,24g/d plus FS Tablets,0.9 g/d),50 patients in each *** the enrollment and 4-week treatment,complete blood count,serum iron indexes were *** events,liver and renal functions,as well as blood coagulation were *** pharmacology was conducted to identify the active ingredients and explore the potential mechanisms of ***:Ten(20%)and 7(14%)participants discontinued the therapy due to gastrointestinal symptoms in oral iron and combination treatment *** 3 groups showed elevated *** patients in the iron group exhibited typically elevated in serum iron and ferritin and decreased in total iron-binding *** change in iron indexes was observed in BYP *** patients in the combination treatment group neither showed significant changes in serum ferritin nortotal iron-binding *** significant adverse reactions were observed in the BYP *** network pharmacology identified 27 bioactive compounds and 145 targets of BYP on gynecological *** processes and pathways including regulation of inflammation,hormone,angiogenesis and hemostasis,responsetodecreased oxygen levels,effects on myeloma cell,and responseto metal ions were ***:BYP contributes to the practical improvement on gynecological anemia potentially through multi-target mechanisms and optimized iron re-distribution.

关键词： Chinese medicine Buxue Yimu Pills gynecological anemia network pharmacology multi-target mechanism

Effect of Half-dose and Standard-dose Conjugated Equine Estrogens Combined with Natural Progesterone or Dydrogesterone on Components of Metabolic Syndrome in Healthy Postmenopausal Women： A Randomized Controlled Trial

在线全文

学校读者我要写书评

暂无评论

Chinese Medical Journal 2016年第23期129卷 2773-2779页

作者： Wei Xue yan Deng yan-fang wang Ai-Jun Sun Department of Obstetrics and Gynecology Peking Union Medical College Hospital Peking Union Medical College Chinese Academy of Medical Science~ Beijing 100730 China

Background： Menopausal hormone therapy （MHT） has been proven to have beneficial effects on several components of metabolic syndrome. However, the effects vary according to different regimens, dosages, and duration of MHT. The aim of the study was to evaluate the effect of standard-dose 0.625 mg conjugated equine estrogen （CEE） and half-dose 0.3 mg CEE daily with different progestogens in a continuous sequential regimen on postmenopausal metabolic parameters in generally healthy postmenopausal women. Methods： A prospective, open-label, randomized controlled clinical trial was conducted between February 2014 and December 2015. Totally 123 Chinese postmenopausal women with climacteric symptoms were included in this study and were randomly assigned to three groups： Group A received CEE 0.3 mg/micronized progesterone （MP） 100 mg daily; Group B received CEE 0.625 mg/MP 100 mg daily; and Group C received CEE 0.625 mg/dydrogesterone 10 mg daily. Drugs were given in a continuous sequential pattern. The duration of treatment was 12 months. Clinical, anthropometrical, and metabolic variables were measured. Data were analyzed according to intention-to-treat analysis, using Student＇s t-test and analysis of variance. Results： A total of 107 participants completed the 12-month follow-up and were included in the data analysis. At 12 months of treatment, high-density lipoprotein cholesterol and apolipoprotein A significantly increased, and low-density lipoprotein cholesterol, fasting glucose, and glycosylated hemoglobin significantly decreased in Groups B and C, compared with baseline （all P 〈 0.05）. Among the three groups, only Group C showed significantly increased triglycerides compared with baseline （ 1.61 ± 0.80 mmol/L vs. 1.21 ± 0.52 mmol/L, P 0.026）. Each group showed a neutral effect on total cholesterol, lipoprotein A, apolipoprotein B, and fasting insulin levels. No cardiovascular and venous thromboembolic events occurred in the three groups. Conclusions： Among Chinese pos

关键词： Chinese Postmenopausal Women Conjugated Equine Estrogen Dydrogesterone Menopausal Hormone Therapy Micronized Progesterone

The effects of 5-lipoxygenase inhibitor and cysteinyl leukotriene receptor 1 antagonist on 1-methyl-4-phenylpyridine-induced neurotoxicity

在线全文

学校读者我要写书评

暂无评论

中国药理学与毒理学杂志 2016年第10期30卷 1022-1023页

作者： ZHANG Xiao-yan LI Chen-tan wang yan-fang ZHAO Jian-bo WEI Er-qing ZHANG Li-hui Department of Pharmacology Hangzhou Key Laboratory of Medical Neurobiology School of MedicineHangzhou Normal University Biotherapy Center The General Hospital of Beijing Military Command Department of Pharmacology School of MedicineZhejiang University

OBJECTIVE Previously we demonstrated the neuroprotective effect of 5-lipoxygenase(5-LOX)inhibitor as well as cysteinyl leukotriene receptor 1(Cys LT1)antagoniston rotenone-induced microglial activation and neuronal *** this study,we determined the effects of 5-LOX inhibitor zileuton and Cys LT1 antagonist montelukast on neurotoxicity induced by 1-methyl-4-phenylpyridine(MPP+)in an in vitro model of Parkinson disease(PD).METHODS The neurotoxicity of MPP+,a neurotoxin relevant to PD,on the PC12 cells was measured by MTT assay,lactate dehydrogenase(LDH)release and double fluorescence staining with Hoechst/propidiumiodide(PI).The protective effects of 5-LOX inhibitor zileuton and Cys LT1 antagonist montelukast were investigated by the above *** We found that exposure of PC12 cells to MPP+led to a reduced cell viability and an increased level of LDH in a concentration-dependent *** with zileuton and montelukast significantly attenuated viability loss and LDH release in MPP+-treated PC12 ***,MPP+increasednecrotic cell death in PC12 *** of montelukast significantly decreased MPP+-induced cell necrosis in PC12 *** The 5-LOX inhibitor zileuton and Cys LT1 antagonist montelukast have a neuroprotective effects on MPP+-induced neurotoxicity in PC12 *** 5-LOX inhibitor and Cys LT1 antagonist might raise a possibility as potential therapeutic agent for PD and other inflammation-related the central nervous system disorders.

关键词： MPP+ neurotoxicity PC12 cell 5-lipoxygenase cysteinyl leukotriene receptor

Understanding the metabolism of Mycoplasma mycoides subsp. capri in vitro by a transcriptomic analysis

在线全文

学校读者我要写书评

暂无评论

Journal of Integrative Agriculture 2018年第2期17卷 428-435页

作者： wang Xiao-hui wang yan-fang HUANG Hai-bi BAI Fan SHI Xiao-na MA Chang-jiao GAO Yuan ZHANG Jian-hua ZHANG Wen-guang HAO Yong-qing Key Laboratory of Microbiology & Immunology College of Veterinary Medicine Inner Mongolia Agricultural University Foundation Medicine College Baotou Medical School Key Laboratory of Animal Genetics and Genomics Inner Mongolia Agricultural University

It is generally known that the culture for mycoplasma is time-consuming and a variety of nutrients are needed in the culture medium. This brings a lot of difficulties to mycoplasma research and application, including Mycoplasma mycoides subsp. capri（Mmc）. Furthermore, little research on the characteristics of Mmc metabolism has been reported. In this study, Mmc PG3 strain cultures were investigated for dynamic gene expression. Culture samples were harvested during logarithmic phase（PG3-1）, stationary phase（PG3-2）, decline phase（PG3-3） and late decline phase（PG3-4）. Twelve RNA samples（three replicates for each of the four growth stages considered） from these cultures were collected and sequenced. Paired comparison between consecutive growth phases in the four growth stages showed 45 significant differentially expressed genes（P〈0.01） were linked to PG3 metabolism. The enzymes these genes coded were mainly involved in ATP synthase, pyrimidine metabolism, nicotinate and nicotinamide metabolism, arginine and proline metabolism. Among these, cytidylate kinase, fructose 1,6-bisphosphate aldolases Class II, nicotinate-nucleotide adenylyltransferase and dihydrolipoamide dehydrogenase play a key role in Mmc metabolism. These results provide a baseline to build our understanding of the metabolic pathway of Mmc.

关键词： metabolism Mycoplasma mycoides subsp.capri transcriptomic