Optimization of micellar electrokinetic capillary chromatography method using central composite design for the analysis of components in Yangwei granule

作     者：Shu-fang WANG Hai-yan FANG Hai-bin QU 

作者机构：Pharmaceutical Informatics Institute Zhejiang University Hangzhou 310058 China 

出 版 物：《Journal of Zhejiang University-Science B(Biomedicine & Biotechnology)》 (浙江大学学报（英文版）B辑（生物医学与生物技术）)

年 卷 期：2011年第12卷第3期

页      面：193-200页

核心收录：

学科分类：1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 10[医学] 

基　　金：Project supported by the National Science and Technology Major Project of Ministry of Science and Technology of China(No. 2009ZX09313-036) the Program for New Century Excellent Talents in University of Ministry of Education of China(No.NCET-06-0515) 

主　　题：Micellar electrokinetic capillary chromatography Traditional Chinese medicine Central composite design Yangwei granule 

摘      要：Central composite design(CCD),together with multiple linear regression,was successfully used to optimize the electrophoretic buffer system of micellar electrokinetic capillary chromatography(MEKC) for the determination of albiflorin,paeoniflorin,liquiritin,and glycyrrhizic acid in the traditional Chinese medicine(TCM) prescription,Yangwei *** of sodium deoxycholate(SDC) and borate,and proportions of ammonia,acetonitrile,and methanol were *** total resolutions of peaks between the analytes and their adjacent peaks in real samples were integrated into the evaluation index of separation *** optimum electrophoretic buffer contained 80 mmol/L SDC,20 mmol/L borate,5%(v/v) methanol,0.5%(v/v) ammonia,and 5%(v/v) *** correlation coefficients(R 2 ) between the peak areas and the corresponding concentrations of analytes were greater than *** limits of detection(LODs) (S/N=3) of the analytes were 0.97-4.00μg/*** results indicate the superiority of CCD in optimizing the separation conditions of complex samples such as TCM prescriptions.