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Determining the critical size of a rabbit rib segmental bone defect model

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Regenerative Biomaterials 2016年第5期3卷 323-328页

作者： Fengzhen Liu Kun Chen Lei Hou Keyi Li Dawei wang Bin Zhang xiumei wang Liaocheng People’s Hospital Medical College of Liaocheng UniversityLiaocheng 252000P.R.China Tsinghua University Beijing 100084P.R.China

In order to establish and standardize the rabbit rib segmental bone defect model,it is of vital importance to determine rabbit rib critical size defect(CSD).According to the general time needed for spontaneous long-bone regeneration,three-month observation period was set to determine the *** rabbit rib segmental bone defects with different sizes from 1 to 5cm with or without periosteum were performed in the eighth rib of 4-month-old male New Zealand rabbits and underwent Xray examinations at the 4th,8th and 12th weeks *** gross and histological examinations at postoperative week 12 were evaluated,which showed that the critical sizes in the rabbit rib models with and without periosteum were 5 and 2 cm,*** study provides prerequisite data for establishing rabbit rib CSD model and evaluating bonematerials using this model.

关键词： animal test bone critical size defect rib defect periosteum

Enhanced angiogenesis by the hyaluronic acid hydrogels immobilized with a VEGF mimetic peptide in a traumatic brain injury model in rats

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Regenerative Biomaterials 2019年第6期6卷 325-334页

作者： Jiaju Lu Fengyi Guan Fuzhai Cui Xiaodan Sun Lingyun Zhao Ying wang xiumei wang State Key Laboratory of New Ceramics and Fine Processing Key Laboratory of Advanced Materials of Ministry of EducationSchool of Materials Science and EngineeringTsinghua UniversityBeijing 100084China Beijing Center of Neutral Regeneration and Repair Key Laboratory for Neurodegenerative Disease of the Ministry of EducationCapital Medical UniversityBeijing 100069China

Angiogenesis plays an important role in brain injury repair,which contributes to the reconstruction of regenerative neurovascular niche for promoting axonal regeneration in the lesion *** a major component of developing brain extracellular matrix,hyaluronic acid(HA)has attracted more attention as a supporting matrix for brain *** the present study,HA-KLT hydrogel was developed via modifying HA with a VEGF mimetic peptide of KLT(KLTWQELYQLKYKGI).The characterization of the hydrogel shows that it could provide a porous,three-dimensional scaffold structure,which has a large specific surface area available for cell adhesion and *** with the unmodified HA hydrogel,the HA-KLT hydrogel could effectively promote the attachment,spreading and proliferation of endothelial cells in ***,the pro-angiogenic ability of hydrogels in vivo was evaluated by implanting them into the lesion cavities in the injured rat *** results showed that the hydrogels could form a permissive interface with the host tissues at 4 weeks after ***,they could efficiently inhibit the formation of glial scars at the injured *** HA-KLT hydrogel could significantly increase the expression of endoglin/CD105 and promote the formation of blood vessels,suggesting that HA-KLT hydrogel promoted angiogenesis in ***,the HA-KLT hydrogel has the potential to repair brain defects by promoting angiogenesis and inhibiting the formation of glial-derived scar tissue.

关键词： traumatic brain injury hyaluronic acid angiogenesis VEGF mimetic peptide

60及以下系列超低断面宽基轮胎的结构设计方法

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轮胎工业 2024年第2期44卷 79-81页

作者：张永光熊瑶王秀梅宫金荣李园园邱云学赵菲葛育坤青岛双星轮胎工业有限公司山东青岛266400

介绍60及以下系列超低断面宽基轮胎的结构设计方法。从宽基载重子午线轮胎半成品的结构、材料、宽度及各定位点等方面进行整体设计,重点介绍带束层、胎体、胎圈包布等钢丝帘布部件及胎面、胎侧、三角胶、垫胶等胶部件在材料分布图中的... 详细信息

介绍60及以下系列超低断面宽基轮胎的结构设计方法。从宽基载重子午线轮胎半成品的结构、材料、宽度及各定位点等方面进行整体设计,重点介绍带束层、胎体、胎圈包布等钢丝帘布部件及胎面、胎侧、三角胶、垫胶等胶部件在材料分布图中的厚度及其端点在材料分布图中合理的分布位置。成品轮胎性能试验结果表明,该设计方法可降低轮胎关键部位的生热,有效提高轮胎的耐久性能,减少胎体帘布弯曲、胎圈漏钢丝等问题的发生,明显提升生产一次合格率。

关键词：宽基轮胎载重子午线轮胎结构设计材料分布图

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基于生物材料的多模态组织工程策略

中国材料进展 2023年第7期42卷 525-533,573,574页

作者：杨嘉王秀梅清华大学材料学院再生医学与仿生材料研究所北京100084

作为组织工程三要素之一,生物材料在组织工程和再生医学中的重要作用备受关注。生物材料不仅仅是支撑结构以及细胞和生长因子的递送载体,同时可构建适宜的“再生微环境”,调控干细胞、免疫细胞、组织特异性细胞的细胞行为并激活组织再... 详细信息

作为组织工程三要素之一,生物材料在组织工程和再生医学中的重要作用备受关注。生物材料不仅仅是支撑结构以及细胞和生长因子的递送载体,同时可构建适宜的“再生微环境”,调控干细胞、免疫细胞、组织特异性细胞的细胞行为并激活组织再生潜能。此外,生物材料所具备的本征理化特性、生物活性,以及传递的外源刺激等不同模态的材料学信号均可调控细胞行为,激活基因表达,进而诱导组织再生。近年来,基于仿生设计原则,解码天然细胞外基质的材料学信号并揭示它对于细胞行为的调控作用和分子机制,指导多功能生物材料设计,诱导组织再生修复,已成为组织工程和再生医学研究的共性科学问题。因此,基于组织工程和再生医学策略中生物材料的重要地位,提出了一种基于生物材料的多模态组织工程策略,即设计开发“All-in-One”的多功能生物材料递送多模态的细胞调控信号,协同调控细胞命运,诱导组织再生修复。在此基础上,综合阐述了生物材料负载的材料学信号对于细胞行为的调控作用,并以神经组织工程为例介绍基于生物材料的多模态策略内涵,最后对该策略的前景和挑战进行了展望。

关键词：生物材料多模态组织工程细胞调控信号再生微环境组织再生

Effect of nanoheat stimulation mediated by magnetic nanocomposite hydrogel on the osteogenic differentiation of mesenchymal stem cells

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Science China(Life Sciences) 2018年第4期61卷 448-456页

作者： Zheng Cao Dan wang Yongsan Li Wensheng Xie Xing wang Lei Wao Yen Wei xiumei wang Lingyun Zhao State Key Laboratory of New Ceramics and Fine Processing School of Materials Science and Engineering Tsinghua University Beijing 100084 China School of Materials Science and Engineering Tsinghua University Beijing 100084 China Department of Chemistry Tsinghua University Beijing 100084 China The State Key Laboratory of Chemical Resource Engineering Beijing University of Chemical Technology Beijing 100029 China

Hyperthermia has been considered as a promising healing treatment in bone regeneration. We designed a tissue engineering hydrogel based on magnetic nanoparticles to explore the characteristics of hyperthermia for osteogenic regeneration. This nanocomposite hydrogel was successfully fabricated by incorporating magnetic Fe_3O_4 nanoparticles into chitosan/polyethylene glycol(PEG) hydrogel, which showed excellent biocompatibility and were able to easily achieve increasing temperatures under an alternative magnetic field(AMF). With uniformly dispersed nanoparticles, the composite hydrogel resulted in high viability of mesenchymal stem cells(MSCs), and the elevated temperature contributed to the highest osteogenic differentiation ability compared with direct heat treatment applied under equal temperatures. Therefore, the nanoheat stimulation method using the magnetic nanocomposite hydrogel under an AMF may be considered as an alternative candidate in bone tissue engineering regenerative applications.

关键词：干细胞磁性 Fe3O4 再生应用设计乙二醇温度 AMF

Neurogenic differentiation of human umbilical cord mesenchymal stem cells on aligned electrospun polypyrrole/ polylactide composite nanofibers with electrical stimulation

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Frontiers of Materials Science 2016年第3期10卷 260-269页

作者： Junfeng ZHOU Liang CHENG Xiaodan SUN xiumei wang ShouhongJIN Junxiang LI Qiong WU Key Laboratory of Advanced Materials of Ministry of Education of China School of Materials Science and Engineering Tsinghua University Beijing 100084 China School of Life Sciences Tsinghua University Beijing 100084 China

Adult central nervous system （CNS） tissue has a limited capacity to recover after trauma or disease. Recent medical cell therapy using polymeric biomaterial- loaded stem cells with the capability of differentiation to specific neural population has directed focuses toward the recovery of CNS. Fibers that can provide topographical, biochemical and electrical cues would be attractive for directing the differentiation of stem cells into electro-responsive cells such as neuronal cells. Here we report on the fabrication of an electrospun polypyrrole/polylactide composite nanofiber film that direct or determine the fate of mesenchymal stem cells （MSCs）, via combination of aligned surface topography, and electrical stimulation （ES）. The surface morphology, mechanical properties and electric properties of the film were characterized. Comparing with that on random surface film, expression of neurofilament-lowest and nestin of human umbilical cord mesenchymal stem cells （huMSCs） cultured on film with aligned surface topography and ES were obviously enhanced. These results suggest that aligned topography combining with ES facilitates the neurogenic differentiation of huMSCs and the aligned conductive film can act as a potential nerve scaffold.

关键词： human umbilical cord mesenchymal stem cells neurogenic differentiation conductive composite film electrospun nanofibers electrical stimulation

Directing neural stem cell fate with biomaterial parameters for injured brain regeneration

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Progress in Natural Science:Materials International 2013年第2期23卷 103-112页

作者： Shenglian Yao Xi Liu xiumei wang Antonio Merolli Xiongbiao Chen Fuzhai Cui Institute for Regenerative Medicine and Biomimetic Materials Key Laboratory of Advanced Materials School of Materials Science and Engineering Tsinghua University National Engineering Laboratory for Regenerative Implantable Medical Devices Orthopaedics & Hand Surgery Unit The Catholic University School of Medicine Division of Biomedical Engineering College of Engineering University of Saskatchewan

The discovery of neural stem cells (NSCs), which have the ability to self-renew and differentiate into all types of neural lineages, offers promising prospect for the treatment of brain neurological disorders such as stroke/cerebral ischemia, traumatic brain injury and neurodegenerative disorders. However, only limited number of NSCs could survive or propagate due to tissue inflammation or blood-brain barrier. Therefore, it is necessary to develop an appropriate culture system that highly mimics the natural NSCs niche to direct stem cell fate and behavior for nerve regeneration. Both biophysical and biochemical properties of the NSC niche, including topology, mechanical properties, bioactive molecules, and their spatial and temporal presentations should be considered for the design of functionalized scaffolds, which could not only serve as the delivery vehicles of NSCs but also stimulate specific cellular responses at the molecular level, such as support endogenous or exogenous cells proliferation, migration and homing, even promote the growth of axon at the injured brain site. This review attempts to outline the varieties of biomaterial

关键词： NSCs niche Biomaterial design Brain tissue engineering Nerve regeneration

Elevated Kallistatin promotes the occurrence and progression of non-alcoholic fatty liver disease

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Signal Transduction and Targeted Therapy 2024年第4期9卷 1730-1744页

作者： Zhenzhen Fang Gang Shen Yina wang Fuyan Hong xiumei Tang Yongcheng Zeng Ting Zhang Huanyi Liu Yanmei Li Jinhong wang Jing Zhang Anton Gao Weiwei Qi Xia Yang Ti Zhou Guoquan Gao Department of Biochemistry Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China Department of VIP Medical Center the Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Physical Examination Center the Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 510080China Department of Clinical Laboratory Guangzhou First People's HospitalGuangzhou 510080China Guangdong Key Laboratory of Liver Disease Research the Third Affiliated Hospital of Sun Yat-Sen UniversityGuangzhou 51008China Department of Health Sciences College of Health SolutionsArizona State UniversityTempeUSA Guangdong Engineering&Technology Research Center for Gene Manipulation and Biomacromolecular Products Sun Yat-Sen UniversityGuangzhou 510080China Guangdong Province Key Laboratory of Diabetology Guangzhou 510o80China Guangdong Province Key Laboratory of Brain Function and Disease Zhongshan School of MedicineSun Yat-Sen UniversityGuangzhou 510080China

Non-alcoholic fatty liver disease(NAFLD)is the most common chronic liver disease worldwide,and the development of nonalcoholic steatohepatitis(NASH)might cause irreversible hepatic ***(HLP)is the leading risk factor for *** study aims to illuminate the causative contributor and potential mechanism of Kallistatin(KAL)mediating HLP to NAFLD.221 healthy control and 253 HLP subjects,62 healthy control and 44 NAFLD subjects were *** plasma KAL was significantly elevated in HLP subjects,especially in hypertriglyceridemia(HTG)subjects,and positively correlated with liver ***,KAL levels of NAFLD patients were significantly *** transgenic mice induced hepatic steatosis,inflammation,and fibrosis with time and accelerated inflammation development in high-fat diet(HFD)*** contrast,KAL knockout ameliorated steatosis and inflammation in high-fructose diet(HFruD)and methionine and choline-deficient(MCD)diet-induced NAFLD ***,KAL induced hepatic steatosis and NASH by down-regulating adipose triglyceride lipase(ATGL)and comparative gene identification 58(CGl-58)by LRP6/Gas/PKA/GSK3βpathway through down-regulating peroxisome proliferator-activated receptor(PPARy)and up-regulating kruppel-like factor four(KLF4),***-58 is bound to NF-kB p65 in the cytoplasm,and diminishing CGl-58 facilitated p65 nuclear translocation and TNFa ***,hepatic CGl-58-overexpress reverses NASH in KAL transgenic ***,free fatty acids up-regulated KAL against thyroid hormone in ***,Fenofibrate,one triglyceride-lowering drug,could reverse hepatic steatosis by down-regulating *** results demonstrate that elevated KAL plays a crucial role in the development of HLP to NAFLD and may be served as a potential preventive and therapeutic target.

关键词： GSK3β inflammation elevated

Laves phase hydrogen storage alloys for super-high-pressure metal hydride hydrogen compressors

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Rare Metals 2011年第3期30卷 227-231页

作者： GUO xiumei wang Shumao LIU Xiaopeng LI Zhinian LU Fang MI Jing HAO Lei JIANG Lijun Energy Material and Technology Institute General Research Institute for Nonferrous Metals Beijing 100088 China

Ti-Cr- and Ti-Mn-based alloys were prepared to be low- and high-pressure stage metals for a double-stage super-high-pressure metal hydride hydrogen compressor. Their crystallographic characteristics and hydrogen storage properties were investigated. The alloy pair Ti0.9Zr0.1Mn1.4-Cr0.35V0.2Fe0.05/TiCr1.55Mn0.2Fe0.2 was optimized based on the comprehensive performance of the studied alloys. The product hydrogen with a pressure of 100 MPa could be produced from 4 MPa feed gas when hot oil was used as a heat reservoir.

关键词： hydrogen storage alloys hydrides Laves phase compressors hydrogen