检索结果-南通市图书馆

Role of hsa_circ_0007482 in pterygium development: insights into proliferation, apoptosis, and clinical correlations

引用

International Journal of Ophthalmology(English edition) 2024年第8期17卷 1387-1395页

作者： Li-Yun Zhang xin Liu Shuo Zheng Wen-Qun xi Xue-Ping Wu Dan-Yao Nie Hui-Ling Hu Jian-Tao wang xin-Hua Liu Jing Zhang Shenzhen Eye Hospital Jinan UniversityShenzhen Eye InstituteShenzhen 518000Guangdong ProvinceChina

AIM:To investigate the impact of hsa_circ_0007482 on the proliferation and apoptosis of human pterygium fibroblasts(HPFs)and its correlation with the severity grades of ***:Pterygium and normal conjunctival tissues were collected from the superior area of the same patient’s eye(n=33).The correlation between pterygium severity and hsa_circ_0007482 expression using quantitative reversetranscription polymerase chain reaction(RT-qPCR)were *** distinct siRNA sequences targeting hsa_circ_0007482,along with a negative control sequence,were transfected into *** proliferation was assessed using the cell counting *** levels of Ki67,proliferating cell nuclear antigen(PCNA),Cyclin D1,Bax,B-cell lymphoma-2(Bcl-2),and Caspase-3 were measured via *** staining was employed to detect Ki67 and vimentin *** was evaluated using flow ***:Hsa_circ_0007482 expression was significantly higher in pterygium tissues compared to normal conjunctival tissues(P<0.001).Positive correlations were observed between hsa_circ_0007482 expression and pterygium severity,thickness,and vascular *** of hsa_circ_0007482 inhibited cell proliferation,reducing the mRNA expression of Ki67,PCNA,and Cyclin D1 in ***_circ_0007482 knockdown induced apoptosis,increasing mRNA expression levels of Bax and Caspase-3,while decreasing Bcl-2 expression in ***,hsa_circ_0007482 knockdown attenuated vimentin expression in ***:The downregulation of hsa_circ_0007482 effectively hampers cell proliferation and triggers apoptosis in *** are discernible positive correlations detected between the expression of hsa_circ_0007482 and the severity of pterygium.

关键词： hsa_circ_0007482 circular RNA pterygium cell proliferation apoptosis

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

佛经翻译与中古时期的言意之辩

引用

西北大学学报（哲学社会科学版） 2004年第1期34卷 169-171页

作者：王挺陕西师范大学外国语学院陕西西安710062

佛经翻译与中古文论之间形成了一种长期互动的密切关系。佛经翻译与当时的文学思潮中都存在着言意之辩,二者同受魏晋玄学的影响,因而形成了一种因缘颇深的关系,为魏晋时代文论的繁荣发展和走向独立起到了重要作用。

关键词：佛经翻译中古文学言意之辩魏晋玄学

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

老年前期男性红细胞压积正常参考值与环境因素的曲线模型分析

引用

西南师范大学学报（自然科学版） 2004年第3期29卷 429-433页

作者：王志奎刘倩葛淼陕西师范大学化学与材料科学学院陕西西安710062 西安电子科技大学理学院应用数学系陕西师范大学旅游与环境学院陕西西安710062

为制定中国老年前期男性红细胞压积正常参考值的统一标准提供科学依据,收集了中国270个单位用温氏法测定的28803例健康老年前期男性红细胞压积正常参考值,用曲线回归分析的方法研究了其与海拔高度之间的关系.结果发现:随着海拔高度的逐... 详细信息

为制定中国老年前期男性红细胞压积正常参考值的统一标准提供科学依据,收集了中国270个单位用温氏法测定的28803例健康老年前期男性红细胞压积正常参考值,用曲线回归分析的方法研究了其与海拔高度之间的关系.结果发现:随着海拔高度的逐渐增大,老年前期男性红细胞压积正常参考值按指数律也在逐渐的增大,相关性很显著(r=0 901);推导出了一个曲线回归模型:Y∧=44 8×1 000064x±4 5.如果知道了中国某地的海拔高度,就可以用回归模型估算该地区的老年前期男性红细胞压积正常参考值.依据老年前期男性红细胞压积正常参考值与地理因素的依赖关系把中国分为青藏区、中部区、东部区等3个区.

关键词：红细胞压积正常参考值海拔高度回归分析

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Novel miR-490-3p/hnRNPA1-b/PKM2 axis mediates the Warburg effect and proliferation of colon cancer cells via the PI3K/AKT pathway

引用

World Journal of Gastrointestinal Oncology 2024年第5期16卷 2038-2059页

作者： xiang-Hui Wan Guo-Bing Jin Qun Yang Ji-Long Hu Zhi-Liang Liu Jun Rao Can Wen Peng-Ling Li xi-Mei Yang Bo Huang xiao-Zhong wang Jiangxi Medical College Nanchang UniversityNanchang 330029Jiangxi ProvinceChina Department of Clinical Laboratory Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Jiangxi Key Laboratory of Translational Research for Cancer Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Abdominal Surgery Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Pathology Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Science and Education Section Jiangxi Cancer HospitalNanchang 330029Jiangxi ProvinceChina Department of Clinical Laboratory Jiangxi Children’s HospitalNanchang 330006Jiangxi ProvinceChina Department of Clinical Laboratory The Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina Jiangxi Province Key Laboratory of Laboratory Medicine The Second Affiliated Hospital of Nanchang UniversityNanchang 330006Jiangxi ProvinceChina

BACKGROUND Heterogeneous ribonucleoprotein A1(hnRNPA1)has been reported to enhance the Warburg effect and promote colon cancer(CC)cell proliferation,but the role and mechanism of the miR-490-3p/hnRNPA1-b/PKM2 axis in CC have not yet been *** To investigate the role and mechanism of a novel miR-490-3p/hnRNPA1-b/PKM2 axis in enhancing the Warburg effect and promoting CC cell proliferation through the PI3K/AKT *** Paraffin-embedded pathological sections from 220 CC patients were collected and subjected to immunohistochemical analysis to determine the expression of *** relationship between the expression values and the clinicopathological features of the patients was *** in mRNA expression were analyzed using quantitative real-time polymerase chain reaction,while differences in protein expression were analyzed using western *** proliferation was evaluated using the cell counting kit-8 and 5-ethynyl-2’-deoxyuridine assays,and cell cycle and apoptosis were detected using flow cytometric *** targeted binding of miR-490-3p to hnRNPA1-b was validated using a dual luciferase reporter *** Warburg effect was evaluated by glucose uptake and lactic acid production *** The expression of hnRNPA1-b was significantly increased in CC tissues and cells compared to normal controls(P<0.05).Immunohistochemical results demonstrated significant variations in the expression of the hnRNPA1-b antigen in different stages of CC,including stage I,II-III,and ***,the clinicopathologic characterization revealed a significant correlation between hnRNPA1-b expression and clinical stage as well as T ***1-b was found to enhance the Warburg effect through the PI3K/AKT pathway,thereby promoting proliferation of HCT116 and SW620 ***,the proliferation of HCT116 and SW620 cells was inhibited when miR-490-3p targeted and bound to hnRNPA1-b,effectively blocking the Warburg ***

关键词： Heterogeneous ribonucleoprotein A1-b MiR-490-3p Colon cancer Alternative splicing Warburg effect

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

总体比例的分级Bayes估计

引用

陕西师范大学学报（自然科学版） 2002年第2期30卷 121-122页

作者：王鹏刘新平陕西师范大学数学与信息科学学院陕西西安710062

在总体比例的Bayes估计中 ,引入超先验 ,得到总体比例的多层先验分布 .在此先验下 ,给出总体比例的分级Bayes估计量 .当总体比例值较小时。

关键词：总体比例分级Bayes估计 Bayes估计量多层先验分布分级Bayes估计量样本比例样本容量

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

FOXP3 expression and clinical characteristics of hepatocellular carcinoma

引用

World Journal of Gastroenterology 2010年第43期16卷 5502-5509页

作者： Wei-Hua wang, Jun-Zhi wang, National Center for Safety Evaluation of Drugs, National Institute for the Control of Pharmaceutical and Biological Products, Beijing 100050, china Wei-Hua wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, Biotechnology Center, School of Pharmacy, the Fourth Military Medical university, xi’an 710032, shaanxi Province, china Wei-Hua wang, Chang-Li Jiang, Cun Zhang, Bo Yan, Wei Zhang, Wei Han, Ying-Qi Zhang, State Key Laboratory of Cancer Biology, xi’an 710032, shaanxi Province, china Wei Yan, Department of Pathology, xijing Hospital, The Fourth Military Medical university, xi’an 710032, shaanxi Province, china Yu-Hai Zhang, Department of Health Statistics, The Fourth Military Medical university, xi’an 710032, shaanxi Province, china Jiang-Tao Yang, Department of Pathology, xi’an Gaoxin Hospital, xi’an 710032, shaanxi Province, china National Center for Safety Evaluation of Drugs National Institute for the Control of Pharmaceutical and Biological Products Beijing 100050 China.

AIM: To study the biological and clinical characteristics of transcription factor forkhead box protein 3 (FOXP3) in hepatocellular carcinoma (HCC). METHODS: We analyzed the expression and localization of FOXP3 in HCC tissues and cell lines to evaluate its biological features. The relationship between FOXP3 staining and clinical risk factors of HCC was assessedto identify the clinical characteristics of FOXP3 in HCC. RESULTS: The mRNA and protein expression of FOXP3 were found in some hepatoma cell lines. Immunohistochemical (IHC) analysis of HCC sections revealed that 48% of HCC displayed FOXP3 staining, but we did not find any FOXP3 staining in normal liver tissues and para-tumor tissues. IHC and Confocal analysis showed that the expressions of FOXP3 were mainly present in the nucleus and cytoplasm of tumor cells in tissues or cell lines. In HCC, the distribution of FOXP3 was similar to that of the cirrhosis, but not to the hepatitis B virus. Those findings implicate that FOXP3 staining seems to be associated with the high risk of HCC. CONCLUSION: The clinical characteristics of FOXP3 in HCC warrants further studies to explore its functions and roles in the cirrhosis and development of HCC.

关键词： Forkhead box protein 3 Hepatocellular carcinoma Tumor differentiation Cirrhosis Hepatitis B virus

来源：

维普期刊数据库评论

在线全文

维普期刊数据库

学校读者我要写书评

暂无评论

试论唐人身居陋室的心理及其原因

引用

陕西师范大学学报(哲学社会科学版) 2001年第1期30卷 130-135页

作者：王雪玲陕西师范大学历史文化学院!陕西西安710062

在唐代社会上层 ,当部分人纷纷营建豪宅美第、奢侈浪费渐成风气的同时 ,却有一部分人反其道而行之 ,甘愿选择陋室而居 ,构成唐代特有的陋室文化现象。透过这个现象 ,我们不难发现 ,人在陋室 ,心系天下是部分唐人所追求的一种理想的生活... 详细信息

在唐代社会上层 ,当部分人纷纷营建豪宅美第、奢侈浪费渐成风气的同时 ,却有一部分人反其道而行之 ,甘愿选择陋室而居 ,构成唐代特有的陋室文化现象。透过这个现象 ,我们不难发现 ,人在陋室 ,心系天下是部分唐人所追求的一种理想的生活境界 ;陋室又是部分唐人博取声名、完善自我的一个载体 ;同时它也是唐代法令制度的产物 ,是唐代中后期社会不稳定等诸多因素影响的结果

关键词：唐人住宅住宅文化唐人心理

来源：

同方期刊数据库评论

在线全文

同方期刊数据库

学校读者我要写书评

暂无评论

模糊推理与模糊逻辑

引用

系统工程学报 1998年第2期13卷 1-16页

作者：王国俊陕西师范大学数学研究所

论证了当赋值集为［０，１］时经典命题演算中的定理、重言式、ＭＰ规则以及ＨＳ规则之间关系的不协调性．建立了关于模糊命题演算的准形式演绎系统，证明了相应的可靠性定理．修正了模糊取式（ＦｕｚｚｙＭｏｄｕｓＰｏｎｅｎｓ，ＦＭ... 详细信息

论证了当赋值集为［０，１］时经典命题演算中的定理、重言式、ＭＰ规则以及ＨＳ规则之间关系的不协调性．建立了关于模糊命题演算的准形式演绎系统，证明了相应的可靠性定理．修正了模糊取式（ＦｕｚｚｙＭｏｄｕｓＰｏｎｅｎｓ，ＦＭＰ）与模糊拒取式（ＦｕｚｚｙＭｏｄｕｓＴｏｌｅｎｓ，ＦＭＴ）概念，并明确了ＦＭＰ与ＦＭＴ的模糊逻辑依据．

关键词：模糊推理模糊逻辑准形式演绎系统模糊系统模糊控制

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Identification and effective regulation of scarb1 gene involved in pigmentation change in autotetraploid Carassius auratus

引用

Zoological Research 2024年第2期45卷 381-397页

作者： xi-Dan Xu Yue Zhou Chong-Qing wang Xu Huang Kun Zhang xiao-Wei Xu Li-Wen He xin-Yue Zhang xin-Zhu Fu Ming Ma Qin-Bo Qin Shao-Jun Liu State Key Laboratory of Developmental Biology of Freshwater Fish Engineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education MinistryCollege of Life SciencesHunan Normal UniversityChangshaHunan 410081China Nansha-South china Agricultural university Fishery Research Institute GuangzhouGuangdong 511458China Hunan Yuelu Mountain Science and Technology Co.Ltd.for Aquatic Breeding ChangshaHunan 410081China College of Chemistry and Chemical Engineering Hunan Normal UniversityChangshaHunan 410081China

The autotetraploid Carassius auratus(4nRR,4n=200,RRRR)is derived from whole-genome duplication of Carassius auratus red var.(RCC,2n=100,RR).In the current study,we demonstrated that chromatophores and pigment changes directly caused the coloration and variation of 4nRR skin(red in RCC,brownish-yellow in4nRR).To further explore the molecular mechanisms underlying coloration formation and variation in 4nRR,we performed transcriptome profiling and molecular functional verification in RCC and *** revealed that scarb1,associated with carotenoid metabolism,underwent significant down-regulation in *** editing of this candidate pigment gene provided clear evidence of its significant role in RCC ***,we identified four divergent scarb1 homeologs in 4nRR:two original scarb1 homeologs from RCC and two duplicated ***,three of these homeologs possessed two highly conserved alleles,exhibiting biased and allelespecific expression in the ***,after precise editing of both the original and duplicated scarb1homeologs and/or alleles,4nRR individuals,whether singly or multiply mutated,displayed a transition from brownishyellow skin to a cyan-gray ***,the proportional areas of the cyan-gray regions displayed a gene-dose *** findings illustrate the subfunctionalization of duplicated scarb1,with all scarb1genes synergistically and equally contributing to the pigmentation of *** is the first report concerning the functional differentiation of duplicated homeologs in an autopolyploidfish,substantiallyenrichingour understanding of coloration formation and change within this group of organisms.

关键词： Autopolyploidization Coloration change scarb1 Functional differentiation Genetic changes

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

Influence of He-Ne laser irradiation on the excision repair of cyclobutyl pyrimidine dimers in the wheat DNA

引用

Chinese Science Bulletin 2002年第10期47卷 818-821页

作者： HAN Rong, wang Xunling & YUE MingSchool of Life Science, Northwest university, xi’an 710069, china School of Life Science, Shanxi normal university, Linfen 041004, china School of Life Science, Lanzhou university, Lanzhou 730000, china School of Life Science Northwest University Xi’an China School of Life Science Shanxi Normal University Linfen China School of Life Science Lanzhou University Lanzhou China

Using agarose gel electrophoresis and T4-endo-deoxyribonuclease-V, which is a kind of restriction endonu-clease of cyclobutyl pyrimidine dimer (CPD), the impacts of He-Ne laser (5mW·mm-2) irradiation on DNA excision repair capacity in damaged wheat cells induced by enhanced ultraviolet-B (10.08kJ·m-2·d-1) radiation were studied. The results indicated that the content of endonuclease sensitive sites (ESS) was reduced by He-Ne laser irradiation, which formed in cells irradiated by enhanced ultraviolet-B. With the irradiation of He-Ne laser, the excision of CPDs and the reduction of single strand breaks (SSB) contents which were the endonuclease sensitive sites (ESS) digested by T4-endodeoxyribonuclease-Y had been stimulated in the wheat cells.

关键词： wheat ultraviolet-B He-Ne laser DNA excision repair.

来源：

维普期刊数据库

同方期刊数据库评论

在线全文

学校读者我要写书评

暂无评论

欢迎您,

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案：

请选择收藏分类：

通借通还

欢迎您,

建议与咨询 留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

时间限定

文献类型

馆藏选择

核心期刊

语言

文献类型

帮助

文字说明：

检索规则说明：

检索范例：

分类表

所选分类

限定检索结果

文献类型

馆藏范围

日期分布

学科分类号

主题

机构

作者

语言

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

在线全文

请选择保存的检索档案： 新增检索档案 确定 取消

请选择收藏分类： 新增自定义分类 确定 取消

通借通还

建议与咨询留下您的常用邮箱和电话号码，以便我们向您反馈解决方案和替代方法

请选择保存的检索档案：

请选择收藏分类：