Identification and effective regulation of scarb1 gene involved in pigmentation change in autotetraploid Carassius auratus

作     者：Xi-Dan Xu Yue Zhou Chong-Qing Wang Xu Huang Kun Zhang Xiao-Wei Xu Li-Wen He Xin-Yue Zhang Xin-Zhu Fu Ming Ma Qin-Bo Qin Shao-Jun Liu 

作者机构：State Key Laboratory of Developmental Biology of Freshwater FishEngineering Research Center of Polyploid Fish Reproduction and Breeding of the State Education MinistryCollege of Life SciencesHunan Normal UniversityChangshaHunan 410081China Nansha-South China Agricultural University Fishery Research InstituteGuangzhouGuangdong 511458China Hunan Yuelu Mountain Science and Technology Co.Ltd.for Aquatic BreedingChangshaHunan 410081China College of Chemistry and Chemical EngineeringHunan Normal UniversityChangshaHunan 410081China 

出 版 物：《Zoological Research》 (动物学研究（英文）)

年 卷 期：2024年第45卷第2期

页      面：381-397页

核心收录：

学科分类：0710[理学-生物学] 0908[农学-水产] 09[农学] 

基　　金：supported by the National Natural Science Foundation of China (32172972,U19A2040) Science and Technology Innovation Program of Hunan Province (2021RC4028) Earmarked Fund for China Agriculture Research System (CARS-45) Hunan Provincial Science and Technology Department (2019RS5001) Special Funds for Construction of Innovative Provinces in Hunan Province (2021NK1010) Special Science Found of Nansha-South China Agricultural University Fishery Research Institute,Guangzhou (NSYYKY202305,NSYYKY202306) Aid Program for Science and Technology Innovative Research Team in Higher Educational Institutions of Hunan Province 

主　　题：Autopolyploidization Coloration change scarb1 Functional differentiation Genetic changes 

摘      要：The autotetraploid Carassius auratus(4nRR,4n=200,RRRR)is derived from whole-genome duplication of Carassius auratus red var.(RCC,2n=100,RR).In the current study,we demonstrated that chromatophores and pigment changes directly caused the coloration and variation of 4nRR skin(red in RCC,brownish-yellow in4nRR).To further explore the molecular mechanisms underlying coloration formation and variation in 4nRR,we performed transcriptome profiling and molecular functional verification in RCC and *** revealed that scarb1,associated with carotenoid metabolism,underwent significant down-regulation in *** editing of this candidate pigment gene provided clear evidence of its significant role in RCC ***,we identified four divergent scarb1 homeologs in 4nRR:two original scarb1 homeologs from RCC and two duplicated ***,three of these homeologs possessed two highly conserved alleles,exhibiting biased and allelespecific expression in the ***,after precise editing of both the original and duplicated scarb1homeologs and/or alleles,4nRR individuals,whether singly or multiply mutated,displayed a transition from brownishyellow skin to a cyan-gray ***,the proportional areas of the cyan-gray regions displayed a gene-dose *** findings illustrate the subfunctionalization of duplicated scarb1,with all scarb1genes synergistically and equally contributing to the pigmentation of *** is the first report concerning the functional differentiation of duplicated homeologs in an autopolyploidfish,substantiallyenrichingour understanding of coloration formation and change within this group of organisms.