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Insights into Human Nature and Character of the Epic Through the Lens of Food

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2023年第3期17卷 241-250页

作者： wang chunlin School of Chinese Language and Literature Shanxi UniversityTaiyuan 030006China

Ge Liang's full-length novel Food Is Heaven depicts the legendary lives of figures such as Master Rong Yisheng,Uncle Wuju,the Seventh Young Master Xiang Xikun,and many others,in over 400,000 *** speaking,Food Is Heaven is a fictional work using imaginary characters as its ***,its hidden interplay with important historical events from the 20th century onwards is an undeniable *** non-fictional elements employed by Ge Liang are part of his artistic strategy of achieving literary authenticity in Food Is *** incorporation of major historical figures and events into artistic fiction highlights a certain“epic”quality in the narrative which emerges out of its full engagement with historical essence and historical facts.

关键词： Ge Liang Food Is Heaven epic

Using the Operatic Stage to Showcase the Changing Times and the Vicissitudes of Fate: A Study on The Protagonist by Chen Yan

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Frontiers of Literary Studies in China-Selected Publications from Chinese Universities 2023年第1期17卷 57-67页

作者： wang chunlin School of Chinese Language and Literature Shanxi UniversityTaiyuan 030006China

Chen Yan has emerged as an outstanding novelist thanks to his rich experience in theatrical *** latest novel,The Protagonist,focuses on the heroine Yi Qin'e's forty-year on the stage,and creates a group of characters living in obscurity under the social changes of the times,portraying a broader spectrum of life and society beyond the domain of drama and conveying his profound observations on life,the times,society,and the *** epic artistic nature of the novel and the interplay between the fate of the characters and the social changes taking place at the time in the novel,along with Chen Yan's deep insight into the complexity of humanity are the keys to the successful presentation of the art and ideology of The Protagonist.

关键词： Chen Yan The Protagonist epic nature

Long-term monitoring of the individual self-feeding behavior of rainbow trout Oncorhynchus mykiss

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Journal of Oceanology and Limnology 2019年第1期37卷 344-349页

作者： SHI Ce GAO Xiaolong LIU Ying wang chunlin Key Laboratory of Applied Marine Biotechnology Ministry of Education Faculty of Life Science and Biotechnology Ningbo University Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture Institute of Oceanology Chinese Academy of Sciences College of Marine Technology and Environment Dalian Ocean University

Self-feeding device is extensively used in aquaculture farms, but for salmonids the individual feeding behavior has seldom been continuously observed. In this article, the individual self-feeding behavior of 10 rainbow trout was continuously monitored with a PIT tag record for 50 days with three replicates. The?sh fell into three categories according to their feeding behavior, i.e. high triggering ?sh(trigger behavior more than 25% of the group, HT), low triggering ?sh(1%–25%, LT) and zero triggering ?sh(less than1%). The results showed that in a group of 10 individual 1–2 HT ?sh accounted for most of the self-feeding behavior(78.19%–89.14%), which was far more than they could consume. The trigger frequency of the?sh was signi?cantly correlated with the initial body weight( P 0.05). Cosinor analysis showed that the two HT ?sh in the same group had similar acrophase. Though some of the HT ?sh could be active for 50 d, there were also HT ?sh decreased triggering behavior around 40 d and the high trigger status was then replaced by other ?sh, which was ?rst discovered in salimonds. Interestingly, the growth of the group was not af fected by the alternation triggering ?sh. These results provide evidence that in the self-feeding system the HT ?sh didn't gain much advantage by their frequent self-feeding behavior, and high trigger status of the HT ?sh is not only an individual character but also driven by the demand of the group. In the self-feeding system, the critical individual should be closely monitored.

关键词： self-feeding system individual feeding behavior growth feeding rhythm

Music stimulus has a positive eff ect on survival and development of the larvae in swimming crab Portunus trituberculatus

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Journal of Oceanology and Limnology 2022年第3期40卷 1277-1285页

作者： Zhiming REN Jiechi wang chunlin wang Changkao MU Yangfang YE Ce SHI Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-effi ciency and Healthy Aquaculture Ningbo 315211China

The sound stimulus has a signifi cant impact on marine ***,many studies have focused on the negative impacts of sound on marine *** positive impacts of sound on marine animals,especially on marine invertebrates,were rarely *** the present study,we evaluated the eff ects of music on the survival and development of an economically important marine invertebrate,swimming crab Portunus *** pieces of classical music and a control group,i.e.,Eine Kleine Nachtmusik by Mozart(EK),Bach-Violin Concerto No.1(BV),Anonymous Romanza by Anonymous(AR),and environmental sound(EN)were *** sound pressure received were 163-dB root mean square(RMS)re 1μPa for EK,166-dB RMS re 1μPa for BV,165-dB RMS re 1μPa for AR,44-dB RMS re 1μPa for EN,*** larvae were subject to 3-h classical music transmission every day during the entire larval development *** result showed that BV and AR signifi cantly elevated the cumulative survival rate of the larva from 3.42%±1.77%(EN)to 10.65%±1.69%(BV)and 7.12%±1.37%(AR)respectively(P<0.05).Though music did not aff ect the cumulative development time of larvae from ZoeaⅠto ZoeaⅣ,it signifi cantly increased the molting rate of megalopa from 24.00%±11.78%(EN)to 44.00%±13.06%(AR)and 46.67%±10.50%(BV)(P<0.05).Musical exposure during critical growth intervals may contribute to the survival and development of larval *** an application point of view,music intervention is much economically *** observed music eff ects imply that music could further enhance growth,welfare,and production.

关键词： swimming crab Portunus trituberculatus sound classical music oxygen consumption

Expression and function of WNT4 involved in larvae development and limb regeneration in Portunus trituberculatus

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Journal of Oceanology and Limnology 2021年第1期39卷 306-316页

作者： Zhiming REN Yuanyuan FU Lei LIU Xiao LIU chunlin wang Key Laboratory of Applied Marine Biotechnology Ministry of EducationNingbo UniversityNingbo 315832China Collaborative Innovation Center for Zhejiang Marine High-effi ciency and Healthy Aquaculture Ningbo UniversityNingbo 315832China

The wingless-related integration site(WNT)proteins are a family of secreted glycoproteins that are evolutionarily conserved and are believed to be involved in evolution in vertebrates and *** signaling pathways may be associated with limb regeneration and development in ***,the detail mechanisms remain ***,the distribution of WNT4 in the hepatopancreas,muscle,hemocyte,ganglion,heart,eyestalk,gill tissue,and diff erent larvae development stages of the swimming crab(Portunus trituberculatus)were characterized using immunofl uorescence,real-time PCR,and Western *** cant PtWNT4 expression was detected in heart and *** addition,PtWNT4 was expressed in all larval stages of *** with a dynamic expression pattern,especially in the eyestalk and other organs in the carapace *** injection of WNT4 dsRNA into regenerative limbs signifi cantly decreased PtWNT4 mRNA levels in the eyestalk,heart,and muscle,resulting in 1.9-fold,2.2-fold,and 2.7-fold decreases compared with those detected in the group injected with crab saline(P<0.05),respectively,indicating successful gene ***,expression analysis on the WNT4 using RNAi provides an insight to its functional mechanism during limb regeneration in *** results not only demonstrated the requirement for WNT4 in limb regeneration of Crustaceans,but also suggested its ability to promote larval development at specifi c stages.

关键词： wingless-type MMTV integration site family member-4(WNT4) limb regeneration larvae development Portunus trituberculatus expression pattern RNAi

Postmortem Metabolite Profile Changes of Mud Crab(Scylla paramamosain)Under Different Storage Conditions

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Journal of Ocean University of China 2021年第3期20卷 608-618页

作者： ZHANG Limin GUO Liping MU Changkao YE Yangfang wang chunlin School of Marine Sciences Ningbo UniversityNingbo 315211China

Mud crab(Scylla paramamosain)is one of the most consumed crabs in ***,it is believed that dead mud crabs are not ***,we investigated the postmortem metabolite profile changes of the muscle and hepatopancreas of mud crab associated with storage method using nuclear magnetic resonance-based metabolomics coupled with multivariate data *** results showed that postmortem changes of trimethylamine,organic acids(lactate,formate,and succinate),as well as ATP and its breakdown products are closely affected by the storage *** metabolites can be used as indicators for monitoring loss of crab ***,dead mud crabs preserved an edible quality at least for 4 h at room temperature,12h on ice,and 16 d at−20℃,strongly suggesting that dead crab consumption quality should be determined by *** shouldn’t be discarded without considering the effects of different storage conditions on their freshness.

关键词： mud crab freshness metabolite profiling nuclear magnetic resonance

Enhanced efficiency of cadmium removal by Boehmeria nivea(L.) Gaud. in the presence of exogenous citric and oxalic acids

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Journal of Environmental Sciences 2014年第12期26卷 2508-2516页

作者： Huaying Li Yunguo Liu Guangming Zeng Lu Zhou Xin wang Yaqin wang chunlin wang Xinjiang Hu Weihua Xu College of Environmental Science and Engineering Hunan University Key Laboratory of Environmental Biology and Pollution Control Hunan University Ministry of Education College of Resources and Environmental Science Hunan Normal University

Boehmeria nivea（L.） Gaud. is a potential candidate for the remediation of Cd contaminated sites. The present investigation aims to explore Cd tolerance threshold and to quickly identify the role of exogenous organic acids in Cd uptake and abiotic metal stress *** Cd levels（0–10 mg/L） resulted in an obvious rise in Cd accumulation, ranging from268.0 to 374.4 in root and 25.2 to 41.2 mg/kg dry weight in shoot, respectively. Citric acid at1.5 mmol/L significantly facilitated Cd uptake by 26.7% in root and by 1-fold in shoot,respectively. Cd translocation efficiency from root to shoot was improved by a maximum of66.4% under 3 mmol/L of oxalic acid. Citric acid exhibited more prominent mitigating effect than oxalic acid due to its stronger ligand affinity for chelating with metal and avoiding the toxicity injury of free Cd ions more efficiently. The present work provides a potential strategy for efficient Cd remediation with B. nivea.

关键词： Heavy metal Phytoremediation Lipid peroxidation Antioxidant defense Translocation factor

Phospholipid Compositions in Portunus trituberculatus Larvae at Different Developmental Stages

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Journal of Ocean University of China 2022年第1期21卷 152-162页

作者： LU Zhibin SHI Ce LIU Lei MU Changkao YE Yangfang wang chunlin Key Laboratory of Applied Marine Biotechnology Ningbo UniversityChinese Ministry of EducationNingbo 315211China Collaborative Innovation Center for Zhejiang Marine High-Efficiency and Healthy Aquaculture Ningbo 315211China

Phospholipids are used to improve the growth and survival of Portunus trituberculatus,a widely cultured crab species in ***,only total phospholipids or several classes are applied in crab *** this study,we employed a targeted lipidomic method to investigate the comprehensive phospholipid composition in *** larvae and reveal the changing phospholipid profile over the larval *** showed that *** larvae contain 112 phospholipid species belonging to 10 phospholipid classes,in which phosphatidylcholine(PC)and phosphatidylethanolamine(PE)species are the most abundant,and PC,PE,phosphatidic acid,and phosphatidylserine(PS)are with high *** levels of all phospholipids significantly changed with larval development,which was highlighted by the downward parabolic changes in PE,phosphatidylglycerol,phosphatidylino-sitol,PS,lysophosphatidic acid,and sphingomyelin *** addition,nearly all phospholipid species were depleted at the M stage,which probably contributed to the mass mortality of crab *** findings on the composition and alterations of phospholipids in *** larvae provide novel perspectives for the targeted supplementation of phospholipids in crab *** work also highlights the use of targeted UHPLC-MS lipidomics in understanding the changes of phospholipids during crab development.

关键词： Portunus trituberculatus larval development phospholipid UHPLC-MS

Dynamic metabolite alterations of Portunus trituberculatus during larval development

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Journal of Oceanology and Limnology 2019年第1期37卷 361-372页

作者： SHI Ce ZENG Tinglan LI Ronghua wang chunlin YE Yangfang MU Changkao Key Laboratory of Applied Marine Biotechnology Ningbo University Chinese Ministry of Education Collaborative Innovation Center for Zhejiang Marine High-effi ciency and Healthy Aquaculture

A mass mortality often occurs from molting to the megalopa stage during the larval development of the swimming crab Portunus trituberculatus. Larvae with insuffcient nutrient accumulation during the zoeal stages are probably unable to develop into juvenile swimming crabs. However, the nutritional information such as the primary metabolites is scarce for P. trituberculatus larvae. The aim of this work is to obtain an insight into the metabolite traits of P. trituberculatus at early developmental stages. 1 H nuclear magnetic resonance spectroscopy coupled with multivariate data analysis was used to determine how the metabolite pro?les shift during larval development in P. trituberculatus. Our results show that the trend of total metabolites exhibited a rise from zoea 1 to zoea 3, followed by a drop from zoea 4 to megalopa and recovery during the ?rst juvenile stage. A large-scale depletion of total metabolites in the zoea 4 and megalopa stages suggests a deep depression of metabolic activity, which may be linked to the mass mortality from molting to the megalopa stage. These ?ndings provided essential metabolic information about the larval development of P. trituberculatus and important clues for understanding the nutritional requirements of P. trituberculatus in early developmental stages.

关键词： Portunus trituberculatus larval development metabolite phenotype nuclear magnetic resonance(NMR)