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The Pichia pastoris enzyme production platform:From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass

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Journal of Bioresources and Bioproducts 2024年第1期9卷 43-57页

作者： Korbinian Sinzinger Ulrike Obst Samed Güner Manuel Döring Magdalena Haslbeck Doris Schieder volker Sieber Chair of Chemistry of Biogenic Resources Campus Straubing for Biotechnology and SustainabilityTechnical University of MunichStraubing 94315Germany Catalysis Research Center Technical University of MunichGarching 85748Germany SynBiofoundry@TUM Technical University of MunichStraubing 94315Germany School of Chemistry and Molecular Biosciences The University of QueenslandSt.Lucia 4072Australia

The demand for industrial enzymes is continually rising,fueled by the growing need to shift towards more sustainable industrial ***,making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a ***,the production of the enzymes themselves comes with unavoidable impacts,e.g.,the need to utilize secondary ***,we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris *** demonstrate these capabilities by producing a phytase secreted by *** that is grown on cyanobacterium hydrolysate and buffered glycerol-complex(BMGY)medium,with genetic expression conditions identified by high-throughput screening of a randomized se-cretion *** our best-performing strain is grown in a fed-batch fermentation on BMGY,we reach over 7000 U/mL in three days.

关键词： Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris

Regenerative capacity of Müller cells and their modulation as a tool to treat retinal degenerations

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Neural Regeneration Research 2023年第1期18卷 139-140页

作者： Federica M.Conedera volker Enzmann Advanced Microscopy Program Center for Systems BiologyMassachusetts General HospitalBostonMAUSA Wellman Center for Photomedicine Massachusetts General HospitalBostonMAUSA(Conedera FM)Department of OphthalmologyUniversity HospitalUniversity of BernBernSwitzerland Department of BioMedical Research University of BernBernSwitzerland

Vision is one of our most precious senses,and its impairment has a high socio-economic *** the industrialized world,degenerative diseases of the retina lead to vision loss,particularly among the *** degenerations include,for instance,retinitis pigmentosa,age-related macular degeneration,and diabetic *** treatments are evolving to manage late-stage symptoms of retinal degenerations,no effective therapies to recover vision loss *** degeneration often involves loss or damage to specialized neural cells,such as photoreceptors,and their death stimulates the activation and proliferation of Müller cells(Salman et al.,2021).

关键词： degeneration diseases impairment

Practice guidance documents for the diagnosis and management of non-alcoholic fatty liver disease-recent updates and open questions

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Hepatobiliary Surgery and Nutrition 2023年第5期12卷 780-784页

作者： volker M.Lauschke Dr Margarete Fischer-Bosch Institute of Clinical Pharmacology StuttgartGermany Department of Physiology and Pharmacology Karolinska InstitutetStockholmSweden University Tübingen TübingenGermany

Primarily driven by an increasingly sedentary lifestyle and hypercaloric nutritionally imbalanced diets,non-alcoholic fatty liver disease(NAFLD)has emerged as the most prevalent liver disease in the US and *** constitutes an umbrella term that includes a spectrum of disease from non-alcoholic fatty liver(NAFL)to non-alcoholic steatohepatitis(NASH),NASH fibrosis and NASH cirrhosis that are all characterized by≥5%of all hepatocytes being steatotic in patients with little alcohol intake and no apparent alternative causes.

关键词： Guidelines non-alcoholic steatohepatitis(NASH) fibrosis individualized medicine

A machine-learned interatomic potential for silica and its relation to empirical models

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npj Computational Materials 2022年第1期8卷 822-833页

作者： Linus C.Erhard Jochen Rohrer Karsten Albe volker L.Deringer Institute of Materials Science Technische Universität DarmstadtOtto-Berndt-Strasse 364287DarmstadtGermany Department of Chemistry Inorganic Chemistry LaboratoryUniversity of OxfordOxfordOX13QRUK

Silica(SiO_(2))is an abundant material with a wide range of *** much progress,the atomistic modelling of the different forms of silica has remained a *** we show that by combining density-functional theory at the SCAN functional level with machine-learning-based interatomic potential fitting,a range of condensed phases of silica can be accurately *** present a Gaussian approximation potential model that achieves high accuracy for the thermodynamic properties of the crystalline phases,and we compare its performance(and performance–cost trade-off)with that of multiple empirically fitted interatomic potentials for *** also include amorphous phases,assessing the ability of the potentials to describe structures of melt-quenched glassy silica,their energetic stability,and the high-pressure structural transition to a mainly sixfold-coordinated *** suggest that rather than standing on their own,machine-learned potentials for silica may be used in conjunction with suitable empirical models,each having a distinct role and complementing the other,by combining the advantages of the long simulation times afforded by empirical potentials and the near-quantum-mechanical accuracy of machine-learned *** way,our work is expected to advance atomistic simulations of this key material and to benefit further computational studies in the field.

关键词： potential empirical relation

Functional food additives/ingredients production by engineered Corynebacterium glutamicum

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Systems Microbiology and Biomanufacturing 2023年第1期3卷 110-121页

作者： Katarina Cankar Nadja A.Henke volker F.Wendisch Wageningen Plant Research Wageningen University&Research6708PB WageningenThe Netherlands Genetics of Prokaryotes Center for BiotechnologyBielefeld UniversityBielefeldGermany

Corynebacterium glutamicum is a microbial production host established in the industry 60 years *** is mainly used for production of feed and food amino *** *** strain development has been cutting edge since its discovery,it has been engineered for production of a plethora of valuable *** review will focus on recent developments of *** strain engineering for biotransformation and fermentation processes towards flavor and fragrance molecules as well as pigments and sweeteners.

关键词： Corynebacterium glutamicum Flavors Fragrances Pigments Sweeteners

Are fat NKT cells born that way?

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Cellular & Molecular Immunology 2017年第8期14卷 658-661页

作者： Joshua A vieth Derek B Sant'Angel Child Health Institute of New Jersey Rutgers Robert Wood Johnson Medical SchoolNew BrunswickNJ 08901USA Graduate School of Biomedical Sciences Rutgers Robert Wood Johnson Medical SchoolNew BrunswickNJ 08901USA Department of Pediatrics Rutgers Robert Wood Johnson Medical SchoolNew BrunswickNJ 08901USA

The role of natural killer T(NKT)cells in adipose tissue is a topic of much interest,as reports have suggested a correlation between depletion of the adipose-resident NKT(arNKT)cell population and chronic obesity.1 Fu... 详细信息

关键词： NKT killer obesity

Long-term assist device patients admitted to ICU: Tips and pitfalls

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Journal of Intensive Medicine 2023年第2期3卷 81-88页

作者： Michiel Morshuis Hendrik Fox volker Lauenroth Rene Schramm Heart and Diabetes Center North Rhine-Westphalia Bad Oeynhausen 32545Germany

Left ventricular assist device(LVAD)therapy is well-established in the treatment of end-stage cardiac *** are bridge to transplant(BTT),bridge to candidacy(BTC),bridge to recovery(BTR),and destination therapy(DT).The durability and adverse event(AE)rate of LVADs have improved over the ***,due to donor shortage,the duration of support in the BTT population has increased tremendously;similarly,DT patients are on the device for a long ***,the number of readmissions of long-term LVAD patients has *** cases of severe AEs,intensive care unit(ICU)treatment can be *** complications are the most common ***,embolic or hemorrhagic strokes can occur due to foreign surfaces,acquired von Willebrand syndrome,and anticoagulation *** consequence of the coagulative status,in combination with the continuous flow,are gastrointestinal bleeding ***,in most patients,an isolated LVAD is implanted,and this involves the risk of late right heart *** of pump speed and optimization of the volume status can help solve this *** arrhythmias,pre-existing or de novo after LVAD implantation,can be a life-threatening *** medical therapy or ablation are potential treatment *** for specific LVADs,the Medtronic HeartWare TM ventricular assist device(HVAD)is not manufactured and distributed currently;however,4000 patients are still on the *** thrombosis can occur,wherein thrombolytic therapy is the first-line treatment ***,the HVAD can fail to restart after controller exchange due to technical issues,and precautions must be *** Momentum 3 trial showed superior survival without pump exchange or disabling stroke in patients treated with the HeartMate 3■(HM3;Abbott,Abbott Park,IL,USA)device in comparison to the HeartMate II(HMII).However,in a few cases,a twisted graft or bio debris formation between the outflow graft and bend reli

关键词： Heart failure Left ventricular assist device(LVAD)therapy Adverse events

Capillaric field effect transistors

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Microsystems & Nanoengineering 2022年第2期8卷 59-71页

作者： Claude Meffan Julian Menges Fabian Dolamore Daniel Mak Conan Fee Renwick C.J.Dobson volker Nock Department of Electrical and Computer Engineering University of CanterburyChristchurch 8041New Zealand Department of Microengineering Kyoto University615-8540 KyotoJapan School of Biological Sciences University of CanterburyChristchurch 8041New Zealand Biomolecular Interaction Centre School of Biological SciencesUniversity of CanterburyChristchurch 8041New Zealand School of Product Design University of CanterburyChristchurch 8041New Zealand Department of Biochemistry and Molecular Biology Bio21 Molecular Science and Biotechnology InstituteUniversity of MelbourneParkvilleVIC 3010Australia The MacDiarmid Institute for Advanced Materials and Nanotechnology Wellington 6140New Zealand

Controlling fluid flow in capillaric circuits is a key requirement to increase their uptake for assay *** action off-valves provide such functionality by pushing an occluding bubble into the channel using a difference in capillary ***,we utilized the binary switching mode of this structure to develop a powerful set of fundamental fluidic valving *** this work,we study the transistor-like qualities of the off-valve and provide evidence that these structures are in fact functionally complementary to electronic junction field effect *** view of this,we propose the new term capillaric field effect transistor to describe these types of *** support this conclusion,we present a theoretical description,experimental characterization,and practical application of analog flow resistance *** addition,we demonstrate that the valves can also be *** show modulation of the flow resistance from fully open to pinch-off,determine the flow rate-trigger channel volume relationship and demonstrate that the latter can be modeled using Shockley's equation for electronic ***,we provide a first example of how the valves can be opened and closed repeatedly.

关键词： transistors effect field

Towards a standardisation for digital inputs and outputs of protection functions in IEC 60255 series

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Protection and Control of Modern Power Systems 2022年第1期7卷 382-392页

作者： volker Leitloff Hao Chen Dehui Chen Andrea Bonetti Lei Xu Ahmed Mohamed Carl Byman French Transmission Network Operator Rte ParisFrance State Grid Jiangsu Electric Power Co. Ltd.NanjingChina State Grid Electric Power Institute NanjingChina Royal Institute of Technology StockholmSweden NR Elec-tric Co. Ltd.NanjingChina Scottish and Southern Energy Network(SSEN) GlasgowUK Hitachi Energy VästeråsSweden.

Fully digitalized substations using IEC 61850 process bus are being introduced all over the *** utilities have featured pilot projects,demonstrators or even industrial scale deployment of these Fully Digital Protection,Auto-mation and Control Systems(FD-PACS).Product standards such as profiles for Instrument Transformers have been developed and published(IEC 61869-6 and IEC 61869-9 by TC 38-Instrument Transformers).This raises the question about the standards for digitally interfaced protection *** 2016,IEC TC 95(Measuring relays and protection equipment)charged a working group to investigate this subject and to elaborate recommendations concerning requirements and testing of protection IED with digital inputs and outputs for protection standards(IEC 60255-1xx series).For protection functions,publisher/subscriber based data streams are supposed to comply with IEC 61850 and IEC 61869 *** holds in particular for Sampled Values(SV)representing energising inputs of the protection function,and is also applicable to Generic Object Oriented Substation Event(GOOSE)which can be used for input or output of protection *** attributes of published data depend on the operational and connection status of the function and the hosting *** addition,protection functions have to take into account the information regarding the time synchronisation of the received SV and other *** paper gives an overview of these features and the proposed way to take them into account in the IEC 60255 standard *** describes the progress of WG 2 and relates it to existing standardization documents.

关键词： Process bus Protection function Digital interface SAMU LPIT Standards IEC 61850