The Pichia pastoris enzyme production platform:From combinatorial library screening to bench-top fermentation on residual cyanobacterial biomass
作者机构：Chair of Chemistry of Biogenic ResourcesCampus Straubing for Biotechnology and SustainabilityTechnical University of MunichStraubing 94315Germany Catalysis Research CenterTechnical University of MunichGarching 85748Germany SynBiofoundry@TUMTechnical University of MunichStraubing 94315Germany School of Chemistry and Molecular BiosciencesThe University of QueenslandSt.Lucia 4072Australia
出 版 物：《Journal of Bioresources and Bioproducts》 (生物质资源与工程（英文）)
年 卷 期：2024年第9卷第1期
页 面：43-57页
核心收录：
学科分类：0710[理学-生物学] 071001[理学-植物学] 07[理学]
基 金：Interreg European Commission, EC European Regional Development Fund, ERDF
主 题：Protein secretion Phytase Cyanobacterial biomass Fed-batch fermentation Pichia pastoris
摘 要：The demand for industrial enzymes is continually rising,fueled by the growing need to shift towards more sustainable industrial ***,making efficient enzyme production strains and identifying optimal enzyme expression conditions remains a ***,the production of the enzymes themselves comes with unavoidable impacts,e.g.,the need to utilize secondary ***,we take a more holistic view of bioprocess development and report an integrative approach that allows us to rapidly identify improved enzyme expression and secretion conditions and make use of cyanobacterial waste biomass as feed for supporting Pichia pastoris *** demonstrate these capabilities by producing a phytase secreted by *** that is grown on cyanobacterium hydrolysate and buffered glycerol-complex(BMGY)medium,with genetic expression conditions identified by high-throughput screening of a randomized se-cretion *** our best-performing strain is grown in a fed-batch fermentation on BMGY,we reach over 7000 U/mL in three days.
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