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The Strawberry Fruit Fra a Allergen Functions in Flavonoid Biosynthesis

Molecular Plant 2010年第1期3卷 113-124页

作者： Cristina Munoz Thomas Hoffmann Nieves Medina Escobar Felix Ludemann Miguel A. Botella victoriano valpuesta Wilfried Schwab Departamento de Biologia Molecular y Bioquimica Universidad de Malaga 29071 Malaga Spain Biomolecular Food Technology Technische Universit~t M0nchen Hochfeldweg 1 85354 Freising Germany

The strawberry Fra a I allergen is a homolog of the major birch pollen allergen Bet v 1. It is synthesized by red ripe fruits of Fragaria x ananassa while white fruits of a mutant genotype, which is known to be tolerated by individuals affected by allergy, are devoid of it. Proteomic analyses have shown that Fra a 1 is down-regulated in the tolerated white- fruited genotype along with enzymes of the anthocyanin pigment pathway. In this study, we report the spatial and tem- poral expression of three Fra a genes that encode different isoforms, and the transient RNAi-mediated silencing of the Fra a genes in strawberry fruits of the red-fruited cultivar Elsanta with an ihpRNA construct. As a consequence of reduced levels of Fra a mRNAs, fruits were obtained that produced significantly decreased levels of anthocyanins and upstream metabolites. This effect is consistent with the parallel down-regulation of the phenylalanine ammonia lyase （FaPAL） and to a lesser extent of the chalcone synthase （FaCHS） transcript levels also found in these fruits. In naturally occurring white- fruited genotypes of F. chiloensis and F. vesca, Fra a transcript levels are higher than those of the red-fruited varieties, likely to compensate for the low expression levels of FaPAL and FaCHS in these mutant genotypes. The results demonstrate that Fra a expression is directly linked to flavonoid biosynthesis and show that the Fra a allergen has an essential biological function in pigment formation in strawberry fruit.

关键词： Fragaria flavonoid anthocyanin phenylpropanoid allergen pathogenesis-related proteins.

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Auxin biosynthesis by the YUCCA6 flavin monooxygenase gene in woodland strawberry

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Journal of Integrative Plant Biology 2014年第4期56卷 350-363页

作者： Hong Liu Wei-Fa Xie Ling Zhang victoriano valpuesta Zheng-Wen Ye Qing-Hua Gao Ke Duan Forestry and Fruit Tree Research Institute Shanghai Key Laboratory of Protected Horticultural Technology Shanghai Academy of Agricultural Sciences (SAAS) Shanghai Key Laboratory of Agricultural Genetics and Breeding Institute of Biotechnology Department of Molecular Biology and Biochemistry Universidad de Málaga

Auxin has been regarded as the main signal molecule coordinating the growth and ripening of fruits in strawberry, the reference genomic system for Rosaceae. The mechanisms regulating auxin biosynthesis in strawberry are largely elusive. Recently, we demonstrated that two YUCCA genes are involved in flower and fruit development in cultivated strawberry. Here, we show that the woodland strawberry （Fragaria vesca L.） genome harbors nine loci for YUCCA genes and eight of them encode functional proteins. Transcription pattern in different plant organs was different for all eight FvYUCs. Functionality of the FvYUC6 gene was studied in transgenic strawberry over- expressing FvYUC6, which showed typical high-auxin pheno- types. Overexpression of FvYUC6 also delayed flowering and led to complete male sterility in F. vesta. Additionally, specific repression of FvYUC6 expression by RNA interference signifi- cantly inhibited vegetative growth and reduced plant fertility. The development of leaves, roots, flowers, and fruits was greatly affected in FvYUC6-repressed plants. Expression of a subset of auxin-responsive genes was well correlated with the changes of FvYUC6 transcript levels and free indole-3-acetic acid levels in transgenic strawberry. These observations are consistent with an important role of FvYUC6 in auxin synthesis, and support a main role of the gene product in vegetative and reproductive development in woodland strawberry.

关键词： Auxin biosynthesis Fragaria vesta plant development RNAinterference YUCCA genes

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Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response

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Horticulture Research 2021年第1期8卷 777-789页

作者： Begona Orozco-Navarrete Jina Song Ana Casanal Rosangela Sozzani Victor Flors Jose F.Sanchez-Sevilla Johanna Trinkl Thomas Hoffmann Catharina Merchante Wilfried Schwab victoriano valpuesta Laboratorio de Bioquimica y Biotecnologia Vegetal Instituto de Hortofruticultura Subtropical y Mediterranea(IHSM)Universidad de Malaga-Consejo Superior de Investigaciones CientificasDepartamento de Biologia Molecular y BioquimicaFacultad de CienciasUMAMalagaSpain Department of Plant and Microbial Biology North Carolina State UniversityRaleighNCUSA Metabolic Integration and Cell Signalling Group Plant Physiology SectionDepartment of Ciencias Agrarias y del Medio NaturalUniversitat Jaume ICastelloSpain Departamento de Genomica y Biotecnologia IFAPAMalagaSpain Biotechnology of Natural Products Technische Universität MünchenLiesel-Beckmann-Str.185354 FreisingGermany

The strawberry Fra a 1 proteins belong to the class 10 Pathogenesis-Related(PR-10)*** strawberry,a large number of members have been identified,but only a limited number is expressed in the *** this organ,Fra a 1.01 and Fra a 1.02 are the most abundant Fra proteins in the green and red fruits,respectively,however,their function remains *** know the function of Fra a 1.02 we have generated transgenic lines that silence this gene,and performed metabolomics,RNA-Seq,and hormonal *** studies associated Fra a 1.02 to strawberry fruit color,but the analysis of anthocyanins in the ripe fruits showed no diminution in their content in the silenced *** ontology(GO)analysis of the genes differentially expressed indicated that oxidation/reduction was the most represented biological *** state was not apparently altered since no changes were found in ascorbic acid and glutathione(GSH)reduced/oxidized ratio,but GSH content was reduced in the silenced *** addition,a number of glutathione-S-transferases(GST)were down-regulated as result of Fra a *** highly represented GO category was transport which included a number of ABC and MATE *** the regulatory genes differentially expressed WRKY33.1 and WRKY33.2 were down-regulated,which had previously been assigned a role in strawberry plant defense.A reduced expression of the VQ23 gene and a diminished content of the hormones JA,SA,and IAA were also *** data might indicate that Fra a 1.02 participates in the defense against pathogens in the ripe strawberry fruits.

关键词： straw content fruits

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Identification of quantitative trait loci and candidate genes for primary metabolite content in strawberry fruit

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Horticulture Research 2019年第1期6卷 1638-1654页

作者： JoséG.Vallarino Delphine M.Pott Eduardo Cruz-Rus Luis Miranda Juan J.Medina-Minguez victoriano valpuesta Alisdair R.Fernie JoséF.Sánchez-Sevilla Sonia Osorio Iraida Amaya Department of Molecular Biology and Biochemistry Instituto de Hortofruticultura Subtropical y Mediterránea“La Mayora”University of Málaga–Consejo Superior de Investigaciones Científicas(IHSM-UMA-CSIC)Campus de Teatinos29071 MálagaSpain Max-Planck-Institut für Molekulare Pflanzenphysiologie Am Mühlenberg 114476 Potsdam-GolmGermany Genómica y Biotecnología Centro de MálagaInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)29140 MálagaSpain Ingeniería y Tecnología Agroalimentaria Centro Las Torres-TomejilInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)Alcaládel RíoSevillaSpain Ingeniería y Tecnología Agroalimentaria Centro de HuelvaInstituto Andaluz de Investigación y Formación Agraria y Pesquera(IFAPA)HuelvaSpain

Improvement of nutritional and organoleptic quality of fruits is a key goal in current strawberry breeding *** ratio of sugars to acids is a determinant factor contributing to fruit liking,although different sugars and acids contribute in varying degrees to this complex trait.A segregating F1 population of 95 individuals,previously characterized for several fruit quality characters,was used to map during 2 years quantitative trait loci(QTL)for 50 primary metabolites,L-ascorbic acid(L-AA)and other related traits such as soluble solid content(SSC),titratable acidity(TA),and pH.A total of 133 mQTL were detected above the established thresholds for 44 *** 12.9%of QTL were detected in the 2 years,suggesting a large environmental influence on primary metabolite *** objective of this study was the identification of key metabolites that were associated to the overall variation in SSC and *** it was observed in previous studies,a number of QTL controlling several metabolites and traits were co-located in homoeology group V(HG V).mQTL controlling a large variance in raffinose,sucrose,succinic acid,and L-AA were detected in approximate the same chromosomal regions of different homoeologous linkage groups belonging to HG *** genes for selected mQTL are proposed based on their co-localization,on the predicted function,and their differential gene expression among contrasting F1 progeny ***-seq analysis from progeny lines contrasting in L-AA content detected 826 differentially expressed genes and identified Mannose-6-phosphate isomerase,FaM6PI1,as a candidate gene contributing to natural variation in ascorbic acid in strawberry fruit.

关键词： straw breeding trait

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Involvement of Vesicular Trafficking in Abiotic Stress Tolerance

Involvement of Vesicular Trafficking in Abiotic Stress Toler...

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The Second International Symposium on Plant Neurobiology

作者： Miguel A.Botella Arnaldo Schapire David Posé Abel Rosado victoriano valpuesta Laboratory of Plant Biochemistry and Biotechnology Department of Biochemistry and Molecular Biology Facultad de CienciasUniversidad de MálagaMálagaSpain Laboratory of Plant Biochemistry and Biotechnology Department of Biochemistry and Molecular Biology Facultad de CienciasUniversidad de MálagaMálagaSpain Laboratory of Plant Biochemistry and Biotechnology Department of Biochemistry and Molecular Biology Facultad de CienciasUniversidad de MálagaMálagaSpain Laboratory of Plant Biochemistry and Biotechnology Department of Biochemistry and Molecular Biology Facultad de CienciasUniversidad de MálagaMálagaSpain Laboratory of Plant Biochemistry and Biotechnology Department of Biochemistry and Molecular Biology Facultad de CienciasUniversidad de MálagaMálagaSpain

＜正＞We have identified a number of Arabidopsis mutants that show hypersensitivity to several abiotic stresses. Two of these mutants suggest that membrane trafficking is important for abiotic stress tolerance. The 746 mutant was identified in a screening of plants that showed hypersensitivity to NaCl stress. Identification of the gene altered in this mutant encodes for a protein with homology to synaptotagmins. Synaptotagmins are proteins that share a common structure: an N-terminal transmembrane domain, a

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ON CONSERVATIVE APPROXIMATION BY LINEAR POLYNOMIAL OPERATORS AN EXTENSION OF THE BERNSTEIN'S OPERATOR

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Analysis in Theory and Applications 1995年第1期11卷 62-71页

作者： Francisco-Javier Munoz-Delgado victoriano Ramirez-Gonzalez Paul Sablonniere Universidad de Jaen SpainUniversidad de Granada SpainInstitut National des Sciences Appliquees France

In this work we slwly linear polynomial operators preserving some consecutive i-convexities and leaving in-verant the polynomtals up to a certain degree. First we study the existence of an incompatibility between the conservation of cenain i-cotivexities and the invariance of a space of polynomials. Interpolation properties are obtained and a theorem by Berens and DcVore about the Bernstein's operator ts extended. Finally, from these results a genera'ized Bernstein's operator is obtained.

关键词： LPO ON CONSERVATIVE APPROXIMATION BY LINEAR POLYNOMIAL OPERATORS AN EXTENSION OF THE BERNSTEIN’S OPERATOR

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Antitumor effects of electrochemical treatment

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Chinese Journal of Cancer Research 2013年第2期25卷 223-234页

作者： Héctor Manuel Camué Ciria Maraelys Morales González Lisset Ortíz Zamora Luis Enrique Bergues Cabrales Gustavo victoriano Sierra González Luciana Oliveira de Oliveira Rodrigo Zanella Antonio Carlos Buzaid Orlando Parise Luciana Macedo Brito Cesar Augusto Antunes Teixeira Marina das Neves Gomes Gleyce Moreno Venicio Feo da Veiga Marcos Telló Carla Holandino Research Department National Centre of Applied Electromagnetism Oriente University Pharmacy Department Oriente University Finlay Institute La lisa. La Habana Cuba Veterinary Teaching Hospital Federal University of Rio Grande do Sul. Porto Alegre Sírio e Libanês Hospital Faculty of Pharmacy Medicine Department Federal University of Rio de Janeiro Professor Paulo de Góes Institute of Microbiology Federal University of Rio de Janeiro Pontifical Catholic University of Rio Grande do Sul. Porto Alegre

Electrochemical treatment is an alternative modality for tumor treatment based on the application of a low intensity direct electric current to the tumor tissue through two or more platinum electrodes placed within the tumor zone or in the surrounding areas. This treatment is noted for its great effectiveness, minimal invasiveness and local effect. Several studies have been conducted worldwide to evaluate the anfitumoral effect of this therapy. In all these studies a variety of biochemical and physiological responses of tumors to the applied treatment have been obtained. By this reason, researchers have suggested various mechanisms to explain how direct electric current destroys tumor ceils. Although, it is generally accepted this treatment induces electrolysis, electroosmosis and electroporation in tumoral tissues. However, action mechanism of this alternative modality on the tumor tissue is not well understood. Although the principle of Electrochemical treatment is simple, a standardized method is not yet available. The mechanism by which Electrochemical treatment affects tumor growth and survival may represent more complex process. The present work analyzes the latest and most important research done on the electrochemical treatment of tumors. We conclude with our point of view about the destruction mechanism features of this alternative therapy. Also, we suggest some mechanisms and strategies from the thermodynamic point of view for this therapy. In the area of Electrochemical treatment of cancer this tool has been exploited very little and much work remains to be done. Electrochemical treatment constitutes a good therapeutic option for patients that have failed the conventional oncology methods.

关键词： Direct electric current electrochemical treatment antimmoral treatment tumor tumor electrolysis rumor entropy dissipative structure

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Effect of Aerobic Exercise on Protein Expression in Muscle of Obese Mexican Adolescents: A Proteomic and Bioinformatic Analysis

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Natural Science 2014年第9期6卷 641-650页

作者： Maciste H. Macías-Cervantes Juan M. Guzmán-Flores Katya Vargas-Ortiz Francisco J. Díaz-Cisneros Joel Ramírez-Emiliano victoriano Pérez-Vázquez Departmento de Ciencias Aplicadas al Trabajo División de Ciencias de la Salud Universidad de Guanajuato León México Departmento de Ciencias Médicas División de Ciencias de la Salud Universidad de Guanajuato León México

The beneficial effects of exercise have been recognized for many years yet the molecular mechanisms by which exercise show benefits on health are still elusive. A combination of experimental and bioinformatics approaches can be an invaluable tool to increase the cellular understanding of the molecular mechanisms that underlie the aerobic exercise in obese adolescents. Muscle skeletal proteins were separated by 2D-PAGE and changes in protein expression were revealed by ImageMaster 2D Platinum analysis software. Proteins with expression changes after aerobic exercise were identified by comparison with 2D maps from SWISS-2DPAGE and interactions of proteins were analyzed with STRING and DAVID databases. After aerobic exercise, all participants decreased glucose, insulin, total cholesterol, body mass index and waist circumference. Proteomic differential analysis revealed 10 overexpressed proteins after aerobic training, which correspond to carbonic anhydrase III, beta enolase, creatine kinase, ATP synthase beta subunit, aldolase A, glyceraldehyde-3-phosphate dehydrogenase, triosephosphate isomerase, pyruvate kinase, lactate dehydrogenase and adenylate kinase. Exercise increased glycolysis and oxidative phosphorylation pathways, which is likely to be regulated by ubiquitin c protein. In conclusion, our analysis suggested that obese adolescents show changes in their body mass index, waist circumference and serum glucose after aerobic exercise through improved protein expression patterns that aid, especially, glucose metabolism and increase the oxidative phosphorylation. The most likely explanation for the observed pattern is that ubiquitin c protein keeps the glycolytic enzymes bound within skeletal muscle, obtaining a non-degradative role in cell signaling.

关键词： Glycolysis Oxidative Phosphorylation Ubiquitin C Protein Proteomics Exercise

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Dyslipidaemias and Physical Activity in Children with Cerebral Palsy

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Health 2014年第10期6卷 1030-1037页

作者： Maciste Habacuc Macías-Cervantes Martha Susana Arriola-Nunez Francisco J.Díaz-Cisneros Antonio E.Rivera-Cisneros Jose María de la Roca-Chiapas victoriano Pérez-Vázquez Departamento de Ciencias Medicas Division de Ciencias de la SaludUniversidad de GuanajuatoLeonMexico Universidad del Valle de Atemajac GuadalajaraMexico Departamento de Ciencias Aplicadas al Trabajo Division de Ciencias de la SaludUniversidad de GuanajuatoLeonMexico Division de Ciencias de la Salud Universidad de GuanajuatoLeonMexico Departamento Psicología Division de Ciencias de la SaludUniversidad de GuanajuatoLeonMexico

Cerebral palsy people present movement difficulty and are liable to develop disorders associated with sedentary lifestyles such as dyslipidaemias and cardiovascular diseases. This study aimed to assess physical activity and the prevalence of lipid abnormalities in 29 children with cerebral palsy who were being treated in two care centers in the state of Guanajuato, Mexico. Physical activity was calculated using a survey. Blood glucose, total cholesterol, HDL-C, LDL-C, and triglycerides were determined. Forty-eight percent had at least one dyslipidaemia. The most frequent lipid abnormalities were hypertriglyceridaemia and low HDL-C. High prevalence of the atherogenic index was found. The physical activity of children was lower than recommended for their age. Sixty-two percent attended rehabilitation sessions, 10% attended sports classes, 14% rehabilitation plus sports and 14% did not engage in intentional physical activity. There is high frequency of lipid disorders in children with cerebral palsy. Greater participation in physical activity should be promoted in children to improve their metabolic status and quality of life, especially those suffering from cerebral palsy.

关键词： Hypertriglyceridaemia HDL-C Atherogenic Index

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Intense exercise increases HDL level in children regardless of body weight

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Health 2013年第12期5卷 1949-1953页

作者： Daniel Romero-Gamboa victoriano Pérez-Vázquez Katya Vargas-Ortiz Francisco J. Díaz-Cisneros Claudia Martínez-Cordero Maciste H. Macías-Cervantes Apply Work Science Department Health Science Division University of Guanajuato León México Department of Medical Sciences Health Science Division University of Guanajuato León México Hospital Regional de Alta Especialidad del Bajio León México

Childhood obesity seems to be increasing at a disturbing rate. Exercise and diet are recommended to combat this epidemic. Light and moderate physical activity is associated with health benefits, whereas intense physical activity is associated with normal BMI and low cardiovascular risk. The aim of this study was to assess the metabolic response to a graded exercise test (GXT) in children with different body weight. Eight boys and nine girls underwent a GXT on a treadmill according to Blake protocol. These children were 7-9 years old. The participants were divided into three groups according to their body weight: normal-weight, overweight, and obese. After GXT, lactate and HDL levels increased significantly in all three groups

关键词： Obesity Insulin Physical Activity

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