Down-regulation of Fra a 1.02 in strawberry fruits causes transcriptomic and metabolic changes compatible with an altered defense response
作者机构：Laboratorio de Bioquimica y Biotecnologia VegetalInstituto de Hortofruticultura Subtropical y Mediterranea(IHSM)Universidad de Malaga-Consejo Superior de Investigaciones CientificasDepartamento de Biologia Molecular y BioquimicaFacultad de CienciasUMAMalagaSpain Department of Plant and Microbial BiologyNorth Carolina State UniversityRaleighNCUSA Metabolic Integration and Cell Signalling GroupPlant Physiology SectionDepartment of Ciencias Agrarias y del Medio NaturalUniversitat Jaume ICastelloSpain Departamento de Genomica y BiotecnologiaIFAPAMalagaSpain Biotechnology of Natural ProductsTechnische Universität MünchenLiesel-Beckmann-Str.185354 FreisingGermany
出 版 物：《Horticulture Research》 (园艺研究（英文）)
年 卷 期：2021年第8卷第1期
页 面：777-789页
核心收录：
学科分类：09[农学] 0902[农学-园艺学] 090201[农学-果树学]
基 金：This work was supported by grants from the Spanish Ministry of Economy and Competitiveness(MINECO,BIO2013-44199-R) B.O-N.was supported by a FPI fellowship and C.M.by the RYC program of Spanish Ministry of Economy and Competitiveness The authors would like to thank and acknowledge the support of this work by the EU funded GoodBerry project(funded from the European Union’s Horizon 2020 research and innovation programme under Grant Agreement No 679303)
摘 要：The strawberry Fra a 1 proteins belong to the class 10 Pathogenesis-Related(PR-10)*** strawberry,a large number of members have been identified,but only a limited number is expressed in the *** this organ,Fra a 1.01 and Fra a 1.02 are the most abundant Fra proteins in the green and red fruits,respectively,however,their function remains *** know the function of Fra a 1.02 we have generated transgenic lines that silence this gene,and performed metabolomics,RNA-Seq,and hormonal *** studies associated Fra a 1.02 to strawberry fruit color,but the analysis of anthocyanins in the ripe fruits showed no diminution in their content in the silenced *** ontology(GO)analysis of the genes differentially expressed indicated that oxidation/reduction was the most represented biological *** state was not apparently altered since no changes were found in ascorbic acid and glutathione(GSH)reduced/oxidized ratio,but GSH content was reduced in the silenced *** addition,a number of glutathione-S-transferases(GST)were down-regulated as result of Fra a *** highly represented GO category was transport which included a number of ABC and MATE *** the regulatory genes differentially expressed WRKY33.1 and WRKY33.2 were down-regulated,which had previously been assigned a role in strawberry plant defense.A reduced expression of the VQ23 gene and a diminished content of the hormones JA,SA,and IAA were also *** data might indicate that Fra a 1.02 participates in the defense against pathogens in the ripe strawberry fruits.
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