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Absolute copy number differences of Y chromosomal genes between crossbred (Bos taurus×Bos indicus) and Indicine bulls

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Journal of Animal Science and Biotechnology 2013年第4期4卷 257-263页

作者： Ayan Mukherjee Gulshan Dass Jagan Mohanarao G Moloya Gohain Biswajit Brahma tirtha kumar datta Sachinandan De Animal Genomics Lab Animal Biotechnology Centre National Dairy Research Institute Department of Veterinary Biochemistry College of Veterinary Sciences & Animal Husbandry Central Agricultural University

Background： The Y chromosome in mammal is paternally inherited and harbors genes related to male fertility and spermatogenesis. The unique intra-chromosomal recombination pattern of Y chromosome and morphological difference of this chromosome between Bos taurus and Bos indicus make it an ideal model for studying structural variation, especially in crossbred （Bos taurus x Bos indicus） bulls. Copy Number Variation （CNV） is a type of genomic structural variation that gives information complementary to SNP data. The purpose of this study was to find out copy number differences of four Y chromosomal spermatogenesis-related candidate genes in genomic DNA of crossbred and purebred Indicine bulls. Result： Four Y chromosomal candidate genes of spermatogenesis namely, sex determining gene on Ychromosome （SRY）, DEAD box po/ypeptide 3-Y chromosome （DDX3 Y）, Ubiquidn specific peptidase 9, Y-linked （ usPgY）, testis-specific protein on Y chromosome （TSPY） were evaluated. Absolute copy numbers of Y chromosomal genes were determined by standard curve-based quantitative real time PCR. Copy numbers of SRYand TSPYgenes per unit amount of genomic DNA are higher in crossbred than Indicine bulls. However, no difference was observed in DDX3Yand usPgYgene copy numbers between two groups. Conclusion： The present study demonstrates that the structural organization of Y chromosomes differs between crossbred and Indicine bulls which are reproductively healthy as observed from analysis of semen attributes. The absolute copy numbers of SRY and TSPY genes in unit mass of genomic DNA of crossbred bulls are significantly higher than Indicine bulls. No alteration in absolute copies ofDDX3Yand usPgYgene was found between the genome of crossbred and Indicine bulls. This study suggests that the DDX3Yand USPgYare likely to be single copy genes in the genome of crossbred and Indicine bulls and variation in Y chromosome length between crossbred and Indicine bulls may be due to the copy number variation of

关键词： Absolute copy SRY DDX3Y usPgY TSPY Crossbred

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Effect of exogenous progesterone on cumulus characteristics of buffalo oocytes by allowing passage of more number of sperm through cumulus but not essentially fertilization

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Asian pacific Journal of Reproduction 2018年第2期7卷 79-86页

作者： Madhusmita Panda Asmita Sandeep kumar Purusottam Mishra Mahesh Chandra Sahu Sachinandan De tirtha kumar datta Rakesh kumar Nodal Officer Chief District Veterinary OfficeKadambedaBhadrak756100OdishaIndia Institute of Microbial Technology Council of Scientific and Industrial ResearchChandigarhIndia BSL-4 National Institute of VirologySus RoadPashanPune-411021India Goat Breeding Farm AT/PO-SalapadaDistrict-KeonjharPin code-758020State-OdishaIndia Directorate of Medical Research IMS and SUM HospitalSiksha'O'Anusandhan UniversityK8Kalinga NagarBhubaneswar-751003OdishaIndia Animal Biotechnology Centre National Dairy Research InstituteKarnal132001HaryanaIndia

Objective:To understand the level of progesterone (P4) in different quality of buffalo cumulus oocyte complexes (COCs) and further to evaluate the effect of exogenous P4 supplementation on maturation and subsequent developmental ability of poor quality brilliant cresyl blue (BCB-)***: Progesterone secreted by different quality of buffalo oocytes was estimated by enzyme linked immunosorbent assay and the concentration differences were translated into P4 doses to be incorporated in the maturation medium of BCB-ve COCs followed by expression analysis of genes involved in the cumulus expansion, extracellular matrix disintegration and progesterone receptor signalling. In addition, the study also evaluated the effect of exogenous P4 on sperm-cumulus interaction. Results: More than 10-fold up-regulated expression of progesterone receptor in P4 supplemented oocytes signified that P4 might be acting predominantly through this receptor. Also, exogenous P4 supplementation had significant effect on transcatheter arterial chemoembolization protease regulated by P4-progesterone receptor pathway which in turn had an important role in extracellular matrix disintegration. On the contrary, cumulus expansion genesHAS2,TNFAIP6,AREG were not altered upon P4 supplementation. Also, it was observed that P4 addition did facilitate passage of significantly more number of spermatozoa through P4 treated cumulus cells. Further, incorporation of different doses of P4 did not improve significantly the cleavage and blastocyst rates of BCB-ve ***: Different qualities of buffalo COCs secrete substantially diverse levels of P4, and its supplementation has a role in oocyte maturation via modulation of cumulus characteristics but perhaps not fertilization.

关键词： BCB screening Progesterone Sperm cumulus interaction Fertilizing ability

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Identification of stable internal control genes for accurate normalization of real-time quantitative PCR data in testicular tissue from two breeds of cattle

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Asian pacific Journal of Reproduction 2020年第5期9卷 247-255页

作者： Pradeep Nag Ankur Sharma Elango Kamaraj Arumugam kumaresan tirtha kumar datta Ayyasamy Manimaran Nilendu Paul Sakthivel Jeyakumar Kerekoppa P.Ramesha Theriogenology Laboratory Veterinary Gynaecologyand ObstetricsSouthern Regional Station of ICAR-National Dairy Research InstituteBengaluru–560030 KarnatakaIndia Animal Genomics Laboratory ICAR-National Dairy Research InstituteKarnal–132001 HaryanaIndia Southern Regional Station of ICAR-National Dairy Research Institute Bengaluru–560030 KarnatakaIndia

Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and crossbred ***:Total RNA was isolated from testicular tissue of Zebu and crossbred bulls(n=6 each)between 2-8 years of *** was synthesized,and the quantitative real-time polymerase chain reaction(PCR)was *** cycle threshold values were used for the analysis of the stability of *** different statistical algorithms:geNorm,Normfinder,BestKeeper,and RefFinder,were used to assess the stability of these ***:ATPSF1,HMBS,PPIA,and RPS15A were the most reliable and stable ICGs for Zebu testes,and ATPSF1,RPL23,and PPIA for crossbred ***:A panel of stable ICGs(ATPSF1,HMBS,PPIA,RPS15A for Zebu and ATPSF1,RPL23,and PPIA for crossbred)for normalization of gene expression data in testes samples can be helpful for researchers to conduct functional genomics studies at the testicular level in cattle bulls.

关键词： Cattle bulls Gene expression Internal control genes Normalization qPCR Testis

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Evolution of tectonic landscapes and deformation in the southeast Kumaun and western Nepal Himalaya

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Journal of Mountain Science 2024年第8期21卷 2592-2617页

作者： Khayingshing LUIREI Girish Ch.KOTHYARI Param K GAUTAM Ambar SOLANKI Atul kumar PATIDAR Sentisenla JAMIR Anirudh datta Tanupriya CHOUDHURY Wadia Institute of Himalayan Geology Dehradun 248001India Department of Petroleum Engineering and Earth Sciences University of Petroleum and Energy StudiesDehradun248007India Department of Geology Nagaland UniversityKohima 797004India Academy of Scientific and Innovative Research Ghaziabad 201002India CSE Department Graphic Era deemed to be UniversityDehradun 248002India CSE Department Symbiosis Institute of TechnologySymbiosis International(Deemed University)Pune 412114India

The area of the present investigation’s expanse constitutes the southernmost extent of the southeast Kumaun Himalaya and western Nepal *** approaches have been employed to understand the landforms associated with tectonic deformation,through detailed field investigation supplemented by the geodetic,chronological,and morphometric *** morphogenic expressions of the Main Boundary Thrust(MBT)are reflected in the form of~25 km long E-W trending north dipping fault *** deformation along the strike length of the Himalayan Frontal Thrust(HFT)is noticed in the form of uplifted and incised fill terraces,and strath *** deformation within the fluvial sequences in the study area can be visualized in the form of sheared boulders and pebbles,tilted and faulted terrace ***,the chronological data of fluvial landforms in the study area suggests two major phases of tectonic deformations that have occurred around 58.7±10.8 ka and 3.88±0.4 *** chronology of late-Quaternary landforms advocates that the initial stage of aggradations in the Himalayan foothills commenced around 75.1±0.58 *** aggradational landforms resulted from the diverse depositional regime as evident from the nature of the sediment sequences from clasts dominated to thick mud *** rate of deformation in the southeastern Kumaun and western Nepal Himalaya is±7 mm/yr,as per the data obtained from the Persistent Scatterer Interferometric Synthetic Aperture Radar(PSInSAR).The landform deformation pattern,phase of incision and aggradation,frequent occurrence of landslides,and recent past earthquake activity within the wide zone of the HFT,the MBT,and Ramgarh Thrust suggests that the southernmost front of the Kumaun Himalaya is active and has potential for future *** foothill zone of Himalayan towns are actively growing in terms of population and infrastructural ***,such intradisciplinary studies for tectonically active r

关键词： MBT HFT Fault trace Quaternary deposits sheared boulders

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Therapeutic potential and pharmacological mechanism of visnagin

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Journal of Integrative Medicine 2024年第4期22卷 399-412页

作者： Poonam Yadav Sumeet kumar Singh Sayantap datta Saloni Verma Aarti Verma Arnab Rakshit Anjana Bali Jasvinder Singh Bhatti Amit Khurana Umashanker Navik Department of Pharmacology School of Health SciencesCentral University of PunjabBathindaPunjab 151401India Department of Pharmacological and Pharmaceutical Sciences College of PharmacyUniversity of HoustonHoustonTX 77204-5000USA Department of Human Genetics and Molecular Medicine School of Health SciencesCentral University of PunjabBathindaPunjab 151401India

Visnagin is a furanochromone and one of the most important compound in the Ammi visnaga(L.)Lam(a synonym of Visnaga daucoides Gaertn.)plant,which is used to cure various *** investigations into the bioactive properties of visnagin have been studied to *** literature on visnagin demonstrates its biological properties,including anti-inflammatory,anti-diabetic,and beneficial effects in cardiovascular and renal ***,visnagin improves sperm quality parameters,stimulates steroidogenesis,and increases serum gonadotropins and testosterone levels,while decreasing proinflammatory cytokines,oxidative damage,genomic instability,and it modulates ***,visnagin has emerged as an exciting lead for further research,owing to its potential in various unmet clinical *** current review summarized its basic structure,pharmacokinetics,and pharmacological effects,focusing on its mechanisms of *** review will help to understand the potential of visnagin as an alternative treatment strategy for several diseases and provide insight into research topics that need further exploration for visnagin’s safe clinical use.

关键词： Visnagin Phytochemical Anti-inflammatory Ammi visnaga L. Visnaga daucoides Gaertn Cancer

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Effect of recombinant and native buffalo OVGP1 on sperm functions and in vitro embryo development: a comparative study

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Journal of Animal Science and Biotechnology 2018年第1期9卷 99-110页

作者： Suman Choudhary A.kumaresan Manish kumar Shivani Chhillar Hrudananda Malik Sudarshan kumar Jai K.Kaushik tirtha K.datta Ashok K.Mohanty Animal Biotechnology Centre National Dairy Research Institute Livestock and Production Management Section National Dairy Research Institute

Background： An oviduct-specific glycoprotein, OVGP1, is synthesized and secreted by non-ciliated epithelial cells of the mammalian oviduct which provides an essential milieu for reproductive functions. The present study reports the effects of recombinant buffalo OVGP1 that lacks post-translational modifications, and native Buffalo OVGP1 isolated from oviductal tissue, on frozen-thawed sperm functions and in vitro embryo ***： The proportion of viable sperms was greater（P 〈 0.05） in the recombinant OVGP1-treated group compared to the native OVGP1-treated group at 2 h, 3 h, and 4 h of incubation. The proportion of motile sperms at3 h and 4 h of incubation; and membrane-intact sperms at 4 h was greater（P 〈 0.05） in the native OVGP1-treated group compared to the control and recombinant OVGP1-treated groups. The proportion of capacitated and acrosome-reacted sperms was greater（P 〈 0.05） in the native OVGP1-treated group compared to the recombinant OVGP1 group at 4 h. The rates of cleavage of embryos and their development to the blastocyst stage were greater（P 〈 0.05） in the presence of either native or recombinant OVGP1 in comparison to control at 10 μg/mL concentration as compared to 5 or 20 μg/***： The study suggests that both native and recombinant OVGP1 impart a positive effect on various sperm features and in vitro embryo development. However, native OVGP1 was found to have a more pronounced effect in comparison to recombinant non-glycosylated OVGP1 on various sperm functions except viability. Hence,our current findings infer that glycosylation of OVGP1 might be essential in sustaining the sperm functions but not the in vitro embryo development.

关键词： Blastocyst Capacitation Fertilization Glycoprotein In vitro fertilization Oestrus Semen Sperm

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Heat Transfer and Thermal Characteristics Effects on Moving Plate Impinging from Cu-Water Nanofluid Jet

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Journal of Thermal Science 2020年第1期29卷 182-193页

作者： datta Abanti kumar Sonal HALDER Pabitra Department of Aerospace Engineering and Applied Mechanics Indian Institute of Engineering Science and Technology

The present article is focused on modelling of flow and heat transfer behaviour of Cu-water nanofluid in a confined slot jet impingement on hot moving *** parameters such as various moving plate velocities,nanoparticles at various concentrations,variation in turbulent Reynolds number and jet nozzle to plate distance have been considered to study the flow field and convective heat transfer performance of the *** of distribution of local and average Nusselt number and skin friction coefficients at the plate surface are shown to elucidate the heat transfer and fluid flow *** analysis of both stream function and isotherm contours are carried out to perceive the flow pattern and heat transfer mechanism due to moving *** results revealed that average Nusselt number significantly rises with plate velocity in addition with jet inlet Reynolds *** of the average Nusselt numbers are presented.

关键词： Cu-water nanofluid,multiphase mixture model moving plate jet impingement CFD

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Effects of patch size, disturbances on diversity and structural traits of tropical semi-evergreen forest in the lowland Indo Burma hotspot: implication on conservation of the threatened tree species

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Journal of Mountain Science 2016年第8期13卷 1397-1410页

作者： Koushik MAJUMDAR Badal kumar datta Plant Taxonomy and Biodiversity Laboratory Department of Botany Tripura University

Information on geographic distribution, population and threat status of most globally red listed species in the Indo Burma zone are inadequate. Given the increase in the prevalence of isolated forest patches in the part of this hotspot, evaluation is necessary on how size of the remnant forest patches and on-going disturbances affects structure and diversity of remaining habitats including the population of some globally threatened tree species. Quantitative vegetation inventory by 500 m long and lO m wide （0.5 ha） line transects were randomly set in nine fragmented tropical semi-evergreen forest patches in Tripura, Northeast India. The studied forests patches grouped into small, medium and large based on their respected sizes. Spatial variability was analysed in woody species diversity and structure along the patch size gradients and their response to both patch size and anthropogenic disturbances. Out of 167 species, 13 identified as red-listed species by IUCN under the present inventory. Most of the diversity and structural parameters showed statistically significant positive response with the forest patch size, except for stand dominance, disturbance, sapling density and number of aggregated distribution pattern decreased when patch size increased. In addition, most of the diversity and structural attributes showed negative response with disturbances. Present baseline data would serve as an effective tool for management and protection of this important forest type. Future habitat restoration programme and strategies for relocation of additional population and re-introduction of those globally threatened trees are recommended.

关键词： Forest diversity Semi-evergreen forest Threatened species Population composition IndoBurma zone

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Changes of woody species diversity, horizontal and vertical distribution of stems across interior to outside within a primate rich habitat of Northeast India

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Journal of Forestry Research 2016年第4期27卷 787-798页

作者： Koushik Majumdar Bal Krishan Choudhary Badal kumar datta Plant Taxonomy and Biodiversity Lab. Department of Botany Tripura University

The interior forest of protected area（PA）recover quickly in terms of greater diversity and structural complexity than peripheral and outsides, which may be due to high plant-frugivore interactions in the interior forest than the disturbed outsides. To describe the structural and functional differences in tree communities from interior to outside forests with in small PA, we quantitatively analyzed the vegetation of Trishna Wildlife Sanctuary, a rich primate habitat of Northeast India. Vegetation was sampled at C10 cm girth at breast height by 10 m 9 500 m sized20 line transects（10 ha） laid in the interior（N = 7）,peripheral（N = 7） and outside（N = 6） zones of this sanctuary. All transects were ordinated by Principal Component Analysis based on correlation between diversity and existing disturbance indices. We found significant differences（P ／ 0.05） in taxonomic richness and diversity indices between the habitat. Van diagram confirmed greater unique species richness in the interior zone（64） than peripheral（28） and outside（6） zones. Overall density（ha-1） did not differed across the zones, but basal area（m2ha-1） was significantly（P ／ 0.01） high in peripheral zone. Vertical distribution of stem density was linearly declined across zones（r2adj[ 0.70; P ／ 0.01） with increase in the canopy height. Horizontal distribution of adult stems showed significant inverse quadratic relationship（r2adj[ 0.80; P ／ 0.001）, which suggests very low density of canopy forming voluminous trees in the interior *** protection, restoration and management of interior regions are required to preserve local plant genetic diversity and also to maintain suitable habitat for threatened wildlife.

关键词： interior outside habitat restoration Northeast disturbance quickly inverse preserve quadratic

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Fractional-Order Model of the Disease Psoriasis:A Control Based Mathematical Approach

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Journal of Systems Science & Complexity 2016年第6期29卷 1565-1584页

作者： CAO Xianbing datta Abhirup AL BASIR Fahad ROY Priti kumar College of Science Beijing Technology and Business University Centre for Mathematical Biology and Ecology Department of MathematicsJadavpur University

Autoimmune diseases are generated through irregular immune response of the human body. Psoriasis is one type of autoimmune chronic skin diseases that is differentiated by T-Cells mediated hyper-proliferation of epidermal Keratinocytes. Dendritic Cells and CD8＋ T-Cells have a significant role for the occurrence of this disease. In this paper, the authors have developed a mathematical model of Psoriasis involving CD4＋ T-Cells, Dendritic Ceils, CD8＋ T-Cells and Keratinocyte cell populations using the fractional differential equations with the effect of Cytokine release to observe the impact of memory on the cell-biological system. Using fractional calculus, the authors try to explore the suppressed memory, associated with the cell-biological system and to locate the position of Keratinocyte cell population as fractional derivative possess non-local property. Thus, the dynamics of Psoriasis can be predicted in a better way using fractional differential equations rather than its corresponding integer order model. Finally, the authors introduce drug into the system to obstruct the interaction between CD4＋ T-Cells and Keratinocytes to restrict the disease Psoriasis. The authors derive the Euler-Lagrange conditions for the optimality made through Matlab by developing iterative of the drug induced system. Numerical simulations are schemes.

关键词： Fractional differential equations (FDEs) memory optimal control problem pMHC Pso-riasis.

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