Identification of stable internal control genes for accurate normalization of real-time quantitative PCR data in testicular tissue from two breeds of cattle

作     者：Pradeep Nag Ankur Sharma Elango Kamaraj Arumugam Kumaresan Tirtha Kumar Datta Ayyasamy Manimaran Nilendu Paul Sakthivel Jeyakumar Kerekoppa P.Ramesha Pradeep Nag;Ankur Sharma;Elango Kamaraj;Arumugam Kumaresan;Tirtha Kumar Datta;Ayyasamy Manimaran;Nilendu Paul;Sakthivel Jeyakumar;Kerekoppa P. Ramesha

作者机构：Theriogenology LaboratoryVeterinary Gynaecologyand ObstetricsSouthern Regional Station of ICAR-National Dairy Research InstituteBengaluru–560030 KarnatakaIndia Animal Genomics LaboratoryICAR-National Dairy Research InstituteKarnal–132001 HaryanaIndia Southern Regional Station of ICAR-National Dairy Research InstituteBengaluru–560030 KarnatakaIndia 

出 版 物：《Asian pacific Journal of Reproduction》 (亚太生殖杂志（英文版）)

年 卷 期：2020年第9卷第5期

页      面：247-255页

学科分类：0710[理学-生物学] 1002[医学-临床医学] 0905[农学-畜牧学] 0906[农学-兽医学] 0901[农学-作物学] 100214[医学-肿瘤学] 0902[农学-园艺学] 10[医学] 

基　　金：The present study was carried out under the project“Molecular markers for improving reproduction in cattle and buffaloes”under the funding of Bill and Melinda Gates Foundation USA and Indian Council of Agricultural Research-National Dairy Research Institute 

主　　题：Cattle bulls Gene expression Internal control genes Normalization qPCR Testis 

摘      要：Objective:To assess the stability of 10 candidate internal control genes(ICGs),namely GAPDH,ACTB,RPL23,RPS15A,ATPSF1,GLUT5,HMBS,ATP2B4,PPIA,and BRP to normalize the transcriptional data from testes samples of Zebu and crossbred ***:Total RNA was isolated from testicular tissue of Zebu and crossbred bulls(n=6 each)between 2-8 years of *** was synthesized,and the quantitative real-time polymerase chain reaction(PCR)was *** cycle threshold values were used for the analysis of the stability of *** different statistical algorithms:geNorm,Normfinder,BestKeeper,and RefFinder,were used to assess the stability of these ***:ATPSF1,HMBS,PPIA,and RPS15A were the most reliable and stable ICGs for Zebu testes,and ATPSF1,RPL23,and PPIA for crossbred ***:A panel of stable ICGs(ATPSF1,HMBS,PPIA,RPS15A for Zebu and ATPSF1,RPL23,and PPIA for crossbred)for normalization of gene expression data in testes samples can be helpful for researchers to conduct functional genomics studies at the testicular level in cattle bulls.