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重组大肠杆菌合成聚(3-巯基丙酸)和聚(3-羟基丁酸-3-巯基丙酸)的研究(英文)

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生物工程学报 2003年第2期19卷 195-199页

作者：刘双江 tina lütke-Eversloh Alexander Steinbüchel 中国科学院微生物研究所北京100080 Institute of Microbiology

利用Clostridiumacetobutylicum的丁酸激酶基因 (buk)和磷酸转丁酰基酶基因 (ptb) ,以及Thiocapsapfennigii的PHA合成酶基因 ,设计了一条能够合成多种聚羟基烷酸的代谢途径 ,用构建的质粒转化大肠杆菌 ,获得了重组大肠杆菌菌株。前期的... 详细信息

利用Clostridiumacetobutylicum的丁酸激酶基因 (buk)和磷酸转丁酰基酶基因 (ptb) ,以及Thiocapsapfennigii的PHA合成酶基因 ,设计了一条能够合成多种聚羟基烷酸的代谢途径 ,用构建的质粒转化大肠杆菌 ,获得了重组大肠杆菌菌株。前期的研究表明 ,在合适的前体物条件下 ,该重组大肠杆菌能够合成包括聚羟基丁酸、聚 (羟基丁酸戊酸 )等多种生物聚酯 [liuandSteinb櫣ｃｈｅｌ,*** .Microbiol.6 6 :739 743]。利用该重组大肠杆菌 ,通过生物催化作用合成了 3 巯基丙酸的同型共聚酯 ,同时利用该重组大肠杆菌还获得了含 3 巯基丙酸单体的多种异型共聚物。实验首先研究了 3 巯基丙酸对大肠杆菌生长的影响 ,在此基础上优化了培养过程中添加 3 巯基丙酸的时机和浓度 ,结果表明 ,在实验的条件下 ,细胞合成聚 (3 巯基丙酸 )可达 6 7% (占细胞干重 ) ,合成聚 (3 羟基丁酸— 3 巯基丙酸 ) (分子中 3 巯基丙酸 :3 羟基丁酸 =3:1)可达 2 4 3%。实验进一步研究了同时或分别表达以上 3个基因的重组大肠杆菌合成聚合物的能力 ,结果表明只有当 3个基因同时表达时才能合成聚合物 ,说明 3个基因对合成过程是必须的 ,从而表明了合成途径是按照设计的路线进行的。还通过GC MS、GPC、Ir等手段对合成的化合物进行了定性?

关键词：重组大肠杆菌聚3—巯基丙酸聚3—羟基丁酸—3—巯基丙酸生物合成聚羟基烷酸生物多聚物

Multiple response Optimization of Dimensional Accuracy of Nimonic Alloy Miniature Gear Machined on Wire Edm Using Entropy Topsis Andpareto Anova

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Computer Modeling in Engineering & Sciences 2021年第1期126卷 241-259页

作者： tina Chaudhary Arshad Noor Siddiquee Arindam Kumar Chanda Shafi Ahmad Irfan Anjum Badruddin Zahid A.Khan Department of Mechanical and Automation Engineering Indira Gandhi Delhi Technical University for WomenKashmere Gate110006India Department of Mechanical Engineering Jamia Millia Islamia(A Central University)New Delhi110025India Department of Mechanical and Automation Engineering G.B.Pant Engineering CollegeNew Delhi110020India Mechanical Engineering Department College of EngineeringKing Khalid UniversityAbha-61421AsirSaudi Arabia

The purpose of this research is to obtain the optimum cutting parameters to achieve the dimensional accuracy of Nimonic alloy miniature gear manufactured using Wire *** cutting parameters investigated in this study are current,pulse on time(PON),pulse off time(POFF),wire tension(WT)and dielectric *** glycol,nanopowder of alumina and oxygen are mixed to demineralized water to prepare novel dielectric *** in inner diameter,deviation in outer diameter,deviation in land and deviation in tooth width are considered to check the dimensional *** l_(16) is employed for experimental design and multiple response optimization is performed using Entropy TOPSIS and Pareto *** indicate that pulse on time is the most notable parameter for good dimensional accuracy followed by dielectric fluid,current,pulse off time and wire *** glycol mixed demineralized water is preferred for low dimensional *** optimum WEDM parameters are pulse on time at 20μs,Ethylene glycol mixed demineralized water dielectric fluid,current at 3 A,pulse off time at 4μs,and wire tension at 18 N.

关键词： Miniature gear multiple optimization dimensional accuracy entropy TOPSIS Pareto ANOVA

Trilineage Sequencing reveals Complex TCrβTranscriptomes in Neutrophils and Monocytes Alongside T Cells

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Genomics, Proteomics & Bioinformatics 2021年第6期19卷 926-936页

作者： tina Fuchs Kerstin Puellmann Chunlin Wang Jian Han Alexander W.Beham Michael Neumaier Wolfgang E.Kaminski Institute for Clinical Chemistry University of Heidelberg Medical Faculty MannheimD-68167 MannheimGermany Aesculabor Hamburg D-22769 HamburgGermany irepertoire Inc. HuntsvilleAL 35806USA HudsonAlpha Institute for Biotechnology HuntsvilleAL 35806USA Department of Surgery Hospital of SiegenD-57076 SiegenGermany Ingenium digital diagnostics D-87662 KaltentalGermany

recent findings indicate the presence of T cell receptor(TCr)-based combinatorial immune receptors beyond T cells in neutrophils and monocytes/*** this study,using a semiquantitative trilineage immune repertoire sequencing approach as well as under rigorous bioinformatic conditions,we identify highly complex TCrβtranscriptomes in human circulating monocytes and neutrophils that separately encode repertoire diversities one and two orders of magnitude smaller than that of T *** transcriptomic analyses reveal that neutrophils,monocytes,and T cells express distinct TCrβrepertoires with less than 0.1%overall trilineage repertoire *** comparison shows that in all three leukocyte lineages,the vast majority of the expressed TCrβvariants are *** also find that differentiation of monocytes into macrophages induces dramatic individual-specific repertoire shifts,revealing a surprising degree of immune repertoire plasticity in the monocyte *** results uncover the remarkable complexity of the two phagocyte-based flexible immune systems which until now has been hidden in the shadow of T cells.

关键词： Monocyte Neutrophil T cell TCrβtranscriptome Next-generation sequencing

Efficient first-principles prediction of solid stability:Towards chemical accuracy

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npj Computational Materials 2018年第1期4卷 591-596页

作者： Yubo Zhang Daniil A.Kitchaev Julia Yang tina Chen Stephen T.Dacek rafael A.Sarmiento-Pérez Maguel A.l.Marques Haowei Peng Gerbrand Ceder John P.Perdew Jianwei Sun Department of Physics and Engineering Physics Tulane UniversityNew OrleansLA 70118USA Department of Materials Science and Engineering MITCambridgeMA 02139USA Materials Science Division LBNLBerkeleyCA 94720USA Department of Materials Science and Engineering UC BerkeleyBerkeleyCA 94720USA Institute of Physical Chemistry and National Center for Computational Design and Discovery of Novel Materials Department of ChemistryUniversity of BaselKlingelbergstrBasel 804056Switzerland Institut für Physik Martin-Luther-Universität Halle-WittenbergHalle D-06099Germany Department of Physics Temple UniversityPhiladelphiaPA 19122USA Department of Chemistry Temple UniversityPhiladelphiaPA 19122USA

The question of material stability is of fundamental importance to any analysis of system properties in condensed matter physics and materials *** ability to evaluate chemical stability,i.e.,whether a stoichiometry will persist in some chemical environment,and structure selection,*** crystal structure a stoichiometry will adopt,is critical to the prediction of materials synthesis,reactivity and ***,we demonstrate that density functional theory,with the recently developed strongly constrained and appropriately normed(SCAN)functional,has advanced to a point where both facets of the stability problem can be reliably and efficiently predicted for main group compounds,while transition metal compounds are improved but remain a *** therefore offers a robust model for a significant portion of the periodic table,presenting an opportunity for the development of novel materials and the study of fine phase transformations even in largely unexplored systems with little to no experimental data.

关键词： properties stability chemical

Paleoenvironmental Implications from Biomarker Investigations on the Pliocene lower Sajau lignite Seam in Kasai Area, Berau Basin, Northeast Kalimantan, Indonesia

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Journal of Geoscience and Environment Protection 2015年第5期3卷 140-152页

作者： Khalda Az Zahra Ahmad Helman Hamdani r. tina rosmalina Department of Geological Engineering Padjadjaran University West Java Indonesia research Centre for Chemistry Indonesia Institute of Sciences Indonesia

Pliocene age lignites from lower Sajau seam, from borehole in Berau Basin, Northeast Kalimantan, Indonesia were investigated with respect to organic geochemistry by HPlC, GC and GC/MS. The analysis was conducted to drilling sequence of Kasai Coal Field, Berau Basin which has been applied to identify organic sources and maturity of organic matter. The result of normalized yields of the soluble organic matter (SOM) data indicates saturated and aromatic proportion of hydrocarbon are very low (under 10%), this was reflecting that lignite coal is still in immature stage. Moreover, biomarker hydrocarbon such as diterpenoids was not found from the sample that indicates absence of gymnosperm precursor in the paleo-peat. In contrast, there was great abundance of terpenoid biomarkers including lupane and oleanane showing domination of angiosperms indicate that angiosperm was dominated vegetation source. There also hopanoid biomarkers explaining acidic depositional environment in coal formation and microbes-affected conditions in peat formation process. ratio of Tm/Ts shows paleomire where ratios value of Tm/Ts is in range of oxide condition.

关键词： Paleoenvironment lignite Sajau Berau Basin Biomarker

Chinese Ageing in Minority Cultures： The role of Social Functioning

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Fudan Journal of the Humanities and Social Sciences 2011年第1期4卷 88-107页

作者： Chen Hong--lin Steven M. Shardlow tina l. rochelle

The increasing proportions of older people in the populations of many societies and the patterns of increasing global mobility make social services with ageing ethnic minorities of ever greater importance to social work practitioners. The paper reviews current knowledge and understanding of factors that contribute to social functioning within and outside the family among one migrant group： older ethnic Chinese in Western countries. The findings show that the capacity to maintain a functional social role varies across different groups of Chinese ethnic elders： some are marginalized and excluded from mainstream social engagement on a number of different levels. Some have difl!iculty in accessing public services; some are in a disadvantageous position with regard to wider social connections; and others are empowered in later life in terms of income, social engagement, autonomy and being in a collective, reciprocal relationship. The impact of these factors is complicated by the mixture of the older people＇s cultural heritage, degree of acculturation, socioeconomic status, educational achievements, patterns and history of immigration, refigious beliefs, and family dynamics. Similarly these factors must be understood by social service professionals for providing services to match the needs of ethnic Chinese older people.

关键词： ageing Chinese ethnic minorities social functioning

International narcotics control

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International narcotics control

Increased susceptibility for intrahepatic cholestasis of pregnancy and contraceptive-induced cholestasis in carriers of the 1331T>C polymorphism in the bile salt export pump

1000年

作者： Fraco l. Trujillo and tina r. Contreras editors.

来源：畅想之星图书光盘版（南... 评论

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World Journal of Gastroenterology 2008年第1期14卷 38-45页

作者： Yvonne Meier tina Zodan Carmen lang roland Zimmermann Gerd A Kullak-Ublick Peter J Meier Bruno Stieger Christiane Pauli-Magnus Division of Clinical Pharmacology and Toxicology Department of Internal Medicine University Hospital Zurich Zurich Switzerland Department of Gynecology and Obstetrics University Hospital Zurich Zurich Switzerland Division of Clinical Pharmacology and Toxicology Department of Internal Medicine University Hospital Zurich Zurich Switzerland Clinical Trial Unit University Hospital Basel Basel Switzerland

AIM： To study the association of three common ABCB11 and ABCC2 polymorphisms （ABCB11： 1331T〉C→V444A; ABCC2： 3563T〉A → V1188E and 4544G 〉A → C1515Y） with intrahepatic cholestasis of pregnancy （ICP） and contraceptive-induced cholestasis （CIC）. METHODS： ABCB11 and ABCC2 genotyping data were available from four CIC patients and from 42 and 33 ICP patients, respectively. Allele-frequencies of the studied polymorphisms were compared with those in healthy pregnant controls and Caucasian individuals. Furthermore, serum bile acid levels were correlated with the presence or absence of the 1331 C allele. rESUlTS： The ABCB11 1331T〉C polymorphism was significantly more frequent in cholestatic patients than in pregnant controls： C allele 76.2% （CI, 58.0-94.4） vs 51.3% （CI 35.8-66.7）, respectively （P = 0.0007）; and CC allele 57.1% （CI 36.0-78.3） vs 20% （CI 7.6-32.4）, respectively （P = 0.0065）. All four CIC patients were homozygous carriers of the C allele. In contrast, none of the studied ABCC2 polymorphism was overrepresented in ICP or CIC patients. Higher serum bile acid levels were found in carriers of the 1331CC genotype compared to carriers of the TT genotype. CONClUSION： Our data support a role for the ABCB11 1331T〉C polymorphism as a susceptibility factor for the development of estrogen-induced cholestasis, whereas no such association was found for ABCC2. Serum bile acid and 7-glutamyl transferase levels might help to distinguish ABCB4- and ABCB11-related forms of ICP and CIC.

关键词： Cholestasis of pregnancy Contraceptive-induced cholestasis Bile salt export pump Multidrugresistance associated protein 2 Pharmacogenetics

Statin use and cognitive function in middle-aged adults with type 1 diabetes

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World Journal of Diabetes 2017年第6期8卷 286-296页

作者： Karen A Nunley Trevor J Orchard Christopher M ryan rachel Miller tina Costacou Caterina rosano Department of Epidemiology University of PittsburghPA 15213United States

AIM To test associations between statin use and cognitive impairment in adults with childhood-onset type 1 diabetes(T1D).METHODS In 2010-13, n = 108 middle-aged participants from ongoing observational Pittsburgh Epidemiology of Diabetes Complications Study underwent neurocognitive assessment(mean age and T1 D duration of 49 and 41 years, respectively). All were diagnosed with childhoodonset(i.e., prior to age 18) T1 D between 1950 and 1980 and were seen within one year of diagnosis at Children's Hospital of Pittsburgh. Self-reported statin use(yes/no and if yes, name of statin) was collected biennially from parent study baseline(1986-1988) to time of neurocognitive testing. logistic regression models tested associations between statin use groups and cognitive impairment(defined as having two or more cognitive test scores 1.5SD or worse than published norms) while linear regression models tested associations between statin use groups and cognitive domain z-scores(domains: Verbal IQ, memory, executive function, psychomotor speed, and visuo-construction). All models controlled for education and age. To address confounding by indication, models were repeated using a propensity score for statin *** Of the 108 participants, 51 reported never using statins. Median duration of statin use among the 57 ever users was 6 years. These 57 ever statin users were split to create two groups(≤ or > median years of statin use): 1-6 years(n = 25), and 7-12 years(n = 32). Compared with never users, using statins 1-6 years tripled the odds of cognitive impairment(Or = 3.16; 95%CI: 0.93-10.72; P = 0.06) and using statins 7-12 years almost quintupled the odds of cognitive impairment(Or = 4.84; 95%CI: 1.63-14.44; P = 0.005). Compared with never users, using statins 1-6 or 7-12 years was related to worse performance in the memory domain(β =-0.52; P = 0.003, and-0.39; P = 0.014, respectively). Adjusting for coronary artery disease, low density lipoprotein cholesterol, and Apo E4 statu

关键词： Type 1 diabetes Cognitive impairment Memory Statin use Cohort study