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Echocardiographic mapping of left ventricular resynchronization during cardiac resynchronization therapy procedures

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Chinese Medical Journal 2010年第13期123卷 1645-1651页

作者： CHAN Ngai-yin CHOY Chi-chung CHEUNG Kar-chun lau Chun-leung LO ying-keung CHU Pui-shan YUEN Ho-chuen lau Suet-ting CHOI Yuen-choi Department of Medicine & Geriatrics Princess Margaret Hospital 2-10 Princess Margaret Hospital Road Lai Chi Kok Kowloon Hong Kong China

Background Cardiac resynchronization therapy （CRT） is an effective electrical therapy for patients with moderate to severe heart failure and cardiac dyssynchrony. This study aimed to investigate the degree of acute left ventricular （LV） resynchronization with biventricular pacing （BVP） at different LV sites and to examine the feasibility of performing transthoracic echocardiography （TTE） to quantify acute LV resynchronization during CRT procedure. Methods Fourteen patients with NYHA Class Ⅲ-Ⅳ heart failure, LV ejection fraction 〈35%, QRS duration 〉120 ms and septal-lateral delay （SLD）〉60 ms on tissue Doppler imaging （TDI）, underwent CRT implant. TDI was obtained from three apical views during BVP at each accessible LV site and SLD during BVP was derived. Synchronicity gain index （Sg） by SLD was defined as （I＋（SLD at baseline - SLD at BVP）/SLD at baseline）. Results Seventy-two sites were studied. Positive resynchronization （R＋, Sg〉1） was found in 42 （58%） sites. R＋ was more likely in posterior or lateral than anterior LV sites （66% vs. 36%, P 〈0.001）. Concordance of empirical LV lead implantation sites and sites with R＋ was 50% （7/14）. Conclusions The degree of acute LV resynchronizaUon by BVP depends on LV lead location and empirical implantation of LV lead results in only 50% concordance with R＋. Performing l-rE during CRT implantation is feasible to identify LV sites with positive resynchronization.

关键词： echocardiography cardiac resynchronization therapy heart failure left ventricular lead device implantation optimization

In-depth cDNA Library Sequencing Provides Quantitative Gene Expression Profiling in Cancer Biomarker Discovery

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Genomics,Proteomics & Bioinformatics 2009年第Z1期 1-12页

作者： Wanling Yang, Dingge ying, and Yu-Lung lau Department of Paediatrics and Adolescent Medicine, LKS Faculty of Medicine, The University of Hong Kong, Hong Kong, China.

Quantitative gene expression analysis plays an important role in identifying differentially expressed genes in various pathological states, gene expression regulation and co-regulation, shedding light on gene functions. Although microarray is widely used as a powerful tool in this regard, it is suboptimal quantitatively and unable to detect unknown gene variants. Here we demonstrated effective detection of differential expression and co-regulation of certain genes by expressed sequence tag analysis using a selected subset of cDNA libraries. We discussed the issues of sequencing depth and library preparation, and propose that increased sequencing depth and improved preparation procedures may allow detection of many expression features for less abundant gene variants. With the reduction of sequencing cost and the emerging of new generation sequencing technology, in-depth sequencing of cDNA pools or libraries may represent a better and powerful tool in gene expression profiling and cancer biomarker detection. We also propose using sequence-specific subtraction to remove hundreds of the most abundant housekeeping genes to increase sequencing depth without affecting relative expression ratio of other genes, as transcripts from as few as 300 most abundantly expressed genes constitute about 20% of the total transcriptome. In-depth sequencing also represents a unique advantage of detecting unknown forms of transcripts, such as alternative splicing variants, fusion genes, and regulatory RNAs, as well as detecting mutations and polymorphisms that may play important roles in disease pathogenesis.

关键词： cDNA sequencing sequencing depth expressed sequence tag sequence-specific subtraction biomarker mutation detection

Single incision laparoscopic right hemicolectomy： different approaches

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Chinese Medical Journal 2013年第2期126卷 238-241页

作者： WONG Tak-man DAY Weida KWOK Shu-yan lau ying-yu Patrick YIP Wai-chun Andrew Department of Surgery Kwong Wah Hospital Hong Kong China

Background Single incision laparoscopic colectomy has been performed in recent years, and has been shown to be feasible and safe. This study was to assess the feasibility of single incision laparoscopic right hemicolectomy and to compare the differences in different approaches. Methods This retrospective study included eighteen patients with carcinoma of caecum and ascending colon, undergoing single incision laparoscopic right hemicolectomy. This study also compared single incision laparoscopic right hemicolectomy using different approaches：（1） single incision multiport, （2） single access port and （3） glove port. Results There was no statistical difference in surgical outcomes. Concerning the surgeon＇s satisfaction toward three methods, overcrowding and durability were similar but the single incision multiport was associated with the highest gas-leak and the ＂glove＂ port was associated with poor durability. However, the method of single incision multiport has the lowest average cost of the special trocar or port in each operation. The operative time and blood loss of the operations in this study were comparable to previous publications. Conclusion There was no significant difference between different approaches of single incision laparoscopic right hemicolectomy for colonic cancer in right side colon.

关键词： colonic neoplasms, colectomy instrumentation, laparoscopy methods equipment design

Potential Antiviral Target for SARS-CoV-2:A Key Early Responsive Kinase during Viral Entry

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CCS Chemistry 2022年第1期4卷 112-121页

作者： Siwen Liu Lin Zhu Guangshan Xie Bobo Wing-Yee Mok Zhu Yang Shaofeng Deng Siu-ying lau Pin Chen Pui Wang Honglin Chen Zongwei Cai State Key Laboratory for Emerging Infectious Diseases Department of MicrobiologyThe University of Hong KongPok Fu LamHong Kong SAR 999077 State Key Laboratory of Environmental and Biological Analysis Department of ChemistryHong Kong Baptist UniversityKowloonHong Kong SAR 999077 HKBU Shenzhen Institute of Research and Continuing Education Shenzhen 518000 Beijing Normal University-Hong Kong Baptist University United International College Zhuhai 519087

Currently,there is no effective antiviral medication for coronavirus disease 2019(COVID-19)and the knowledge on the potential therapeutic target is in great *** by a time-course transmission electron microscope(TEM)imaging,we analyzed early phosphorylation dynamics within the first 15 min during severe acute respiratory syndrome coronavirus 2(SARS-CoV-2)viral *** on alterations in the phosphorylation events,we found that kinase activities such as protein kinase C(PKC),interleukin-1 receptor-associated kinase 4(IRAK4),MAP/microtubule affinity-regulating kinase 3(MARK3),and TANK-binding kinase 1(TBK1)were affected within 15 min of *** of the corresponding kinase inhibitors of PKC,IRAK4,and p38 showed significant inhibition of SARS-CoV-2 ***,proinflammatory cytokine production was reduced by applying PKC and p38 *** an acquisition of a combined image data using positiveand negative-sense RNA probes,as well as pseudovirus entry assay,we demonstrated that PKC contributed to viral entry into the host cell,and therefore,could be a potential COVID-19 therapeutic target.

关键词： SARS-CoV-2 viral entry phosphoproteomics early responsive kinase antiviral target PKC

微波消解-电感耦合等离子体质谱法测定丙烯腈-丁二烯-苯乙烯共聚物塑料中的铅、镉、汞、铬、砷

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光谱学与光谱分析 2008年第1期28卷 191-194页

作者：王英锋施燕支张华陈玉红 John lau Steven Wilbur 李平首都师范大学分析测试中心北京100037 安捷伦科技有限公司(中国) 北京100022

建立了微波消解技术-电感耦合等离子体质谱(ICP-MS)法同时测定丙烯腈-丁二烯-苯乙烯共聚物(ABS)塑料中的铅、镉、汞、铬、砷五种元素的方法。对仪器的参数设置、进样系统进行了优化,并对此类塑料样品中上述元素分析的前处理条件,如消解... 详细信息

建立了微波消解技术-电感耦合等离子体质谱(ICP-MS)法同时测定丙烯腈-丁二烯-苯乙烯共聚物(ABS)塑料中的铅、镉、汞、铬、砷五种元素的方法。对仪器的参数设置、进样系统进行了优化,并对此类塑料样品中上述元素分析的前处理条件,如消解体系、消解温度、恒温时间以及酸用量等进行了优化。方法检出限为0.7～6.5ng·g-1;用加标回收的方法评价了该方法的准确性,加标回收率为89.8%～110.8%;样品测定相对标准偏差为2.8%～11.3%。该方法检出限低、精确度好、准确度高、简单快速、无需基体匹配。

关键词： ICP-MS 微波消解丙烯腈-丁二烯-苯乙烯元素

血浆脂蛋白相关磷脂酶A2水平与冠状动脉病变关系的临床研究

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血浆脂蛋白相关磷脂酶A2水平与冠状动脉病变关系的临床研究

作者：刘颖芝（ying Zhi lau）广州医科大学

学位级别：硕士

【研究背景】炎症反应是冠心病的重要特征,且与斑块的进展与稳定性密切相关。研究表明,白细胞介素、肿瘤坏死因子、C反应蛋白等炎症标志物与冠心病的发生、发展与预后有显著相关性。血浆脂蛋白相关磷脂酶A2是一种新的冠心病相关炎症标志... 详细信息

【研究背景】炎症反应是冠心病的重要特征,且与斑块的进展与稳定性密切相关。研究表明,白细胞介素、肿瘤坏死因子、C反应蛋白等炎症标志物与冠心病的发生、发展与预后有显著相关性。血浆脂蛋白相关磷脂酶A2是一种新的冠心病相关炎症标志物,并且可能直接参与冠心病过程。本研究通过检测各组患者血浆脂蛋白相关磷脂酶A2、同型半胱氨酸水平,结合患者病史、临床检查进行相关分析,主要探讨脂蛋白相关磷脂酶A2及同型半胱氨酸水平与冠状动脉病变的相关性。【研究目的】1、探讨血浆脂蛋白相关磷脂酶A2水平与冠脉病变范围的关系,同时检测同型半胱氨酸水平与冠心病的关系。2、比较不同冠脉病变患者之间血浆脂蛋白相关磷脂酶A2水平的差异。【研究方法】选择2015年6月至2015年12月广州医科大学附属第二医院心内科收治的227例患者,分别测定患者血浆标本中脂蛋白相关磷脂酶A2水平、同型半胱氨酸水平、血脂水平,同时行冠脉造影术,分为实验组(当病变管腔狭窄<50%为冠状动脉粥样硬化;病变≥50%狭窄为阳性标准)和对照组(冠脉无狭窄),最后分析年龄、性别、高血压史、糖尿病史、高脂血病史、吸烟史等危险因素、血浆脂蛋白相关磷脂酶A2、同型半胱氨酸水平与冠心病的关系。本研究的所有数据均采用SPSS 21.0软件进行统计分析;定量资料采用x±s描述,定性资料采用n(%)表示。组间比较采用F检验和卡方检验;组间两两比较采用LSD法进行,并报告LSD-t值。多因素分析采用Logistic回归分析方法。【结果】所有患者进行冠脉造影证实,冠脉正常组73人,冠脉异常组154人,其中冠脉粥样硬化35例、单支病变49例、双支病变28例、三支病变42例。冠脉异常组的脂蛋白相关磷脂酶A2平均水平309.60±112.07 ug/L,明显高于冠脉正常者197.30±75.43ug/L,两组之间有显著差异性,P<0.001。将冠脉异常组患者分为4个不同的亚组,冠脉粥样硬化组、单支病变组、双支病变组、三支病变组的脂蛋白相关磷脂酶A2水平分别为:291.49±101.27ug/L、293.84±113.47 ug/L、295.18±132.47 ug/L、352.71±95.26 ug/L,结果发现各个亚组中的脂蛋白相关磷脂酶A2水平均高于对照组,并且差异有统计学意义(P<0.05)。冠脉三支病变组患者的LP-PLA2水平分别高于冠脉粥样硬化组、单支病变及双支病变组,均有统计学意义(P<0.05)。LP-PLA2值水平与冠脉病变范围密切相关,三支病变者增高最明显。Hcy水平与冠脉病变程度无明显相关。【结论】1.冠状动脉阳性病变患者血浆脂蛋白相关磷脂酶A2水平增高。2.血浆脂蛋白相关磷脂酶A2水平增高水平与病变血管范围密切相关,三支病变者增高最明显。

关键词：冠状动脉粥样硬化性心脏病冠状动脉疾病脂蛋白相关磷脂酶A2

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粤英记者会同传的翻译倾向

粤英记者会同传的翻译倾向

作者：刘晓盈（lau Hiu ying）北京外国语大学

学位级别：硕士

莫娜·贝克提出翻译中会出现一些倾向。然而,这种说法是基于以欧洲语言的语言对和可比性低的数据。语料库中的数据常因长度、体裁、时期、正式或非正式和其他因变量而异。因此,本研究旨在以最有限的变量研究正式场合中粤语口译英语的倾... 详细信息

莫娜·贝克提出翻译中会出现一些倾向。然而,这种说法是基于以欧洲语言的语言对和可比性低的数据。语料库中的数据常因长度、体裁、时期、正式或非正式和其他因变量而异。因此,本研究旨在以最有限的变量研究正式场合中粤语口译英语的倾向。为了验证粤语到英语的口译有同样的口译倾向,这个研究比较了粤语的演讲(源语演讲)、香港的英语口译(目标语口译)和香港的英语演讲(目标语演讲)。这些数据摘自中/英政治口译语料库。总体而言,结果表明粤语-英语同声传译中的香港口译英语和自然生产的香港英语很相似,但口译英语仍存在显化倾向。

关键词：翻译普遍性显化粤英同传语料库口译研究

Role of ion channels in maintaining the characteristics of embryonic stem cells

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Role of ion channels in maintaining the characteristics of e...

OBJECT-ORIENTED TECHNOLOGY :

第七届海峡两岸心血管科学研讨会

作者： Sze-ying Ng Yuen-Ting lau Chi-Hou Chin Chun-Kit Wong Suk-ying Tsang Department of Biochemistry Chinese University of Hong Kong

Embryonic stem cells(ESCs)are derived from the inner cell mass of *** can self-renew and are pluripotent,meaning that they can proliferate indefinitely and have the ability to differentiate into all cells in our *** may therefore act as a source of different cell types for future regenerative *** the present study,the role of voltage-gated K(Kv)channels in maintaining the self-renewal and pluripotent characteristics of undifferentiated mouse(m)ESCs was *** channel blocker tetraethylammonium(TEA)was shown to decrease mESC proliferation without affecting cell *** of Kv channels resulted in cell cycle arrest at the G/G *** addition,decreased expression of pluripotent markers such as Oct-4 was detected in Kv channel -blocked *** Kv blockade led to depolarization,and Kv channel-blocked ESCs failed to progress through the cell cycle and had a loss of pluripotency,the present results suggested a possible relationship between the Kv channel functions,membrane potential,cell cycle progression and differentiation potential in *** study shall provide new information about the basic biology of ESCs and provide insights into the possible strategies of controlling ESC self-renewal and differentiation.

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