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Estrogen sulfotransferase regulates inflammatory response and lipid metabolism of human endothelial cells via PPARγ

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中国病理生理杂志 2012年第11期 2036-2037页

作者： XU Ya-li,tian Dai,wang Zheng-yang,li Meng-meng,NING Yan-xia,CHEN Si-feng,li Xiao-bo,YIN lian-hua (Department of Physiology and Pathophysiology,Fudan University Shanghai Medical College,Shanghai 200032,China)

Estrogen sulfotransferase(SULT1E1)is a phase II drug-metabolizing enzyme known to catalyze sulfoconjugation of estrogens.17β-estradiol(E)plays a pivotal role in attenuating endothelial *** be further sulfated to estr... 详细信息

关键词： Estrogen sulfotransferase regulates inflammatory response and lipid metabolism of human endothelial cells via PPAR HUVECs

Transforming growth factor-beta 1 enhances discharge activity of cortical neurons

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Neural Regeneration Research 2025年第2期20卷 548-556页

作者： Zhihui Ren tian li Xueer liu Zelin Zhang Xiaoxuan Chen Weiqiang Chen Kangsheng li Jiangtao Sheng Department of Microbiology and Immunology Guangdong Provincial Key Laboratory of Infectious Diseases and Molecular ImmunopathologyShantou University Medical CollegeShantouGuangdong ProvinceChina Department of Neurosurgery First Affiliated Hospital of Shantou University Medical CollegeShantouGuangdong ProvinceChina

Transforming growth factor-beta 1(TGF-β1)has been extensively studied for its pleiotropic effects on central nervous system *** neuroprotective or neurotoxic effects of TGF-β1 in specific brain areas may depend on the pathological process and cell types ***-gated sodium channels(VGSCs)are essential ion channels for the generation of action potentials in neurons,and are involved in various neuroexcitation-related ***,the effects of TGF-β1 on the functional properties of VGSCs and firing properties in cortical neurons remain *** this study,we investigated the effects of TGF-β1 on VGSC function and firing properties in primary cortical neurons from *** found that TGF-β1 increased VGSC current density in a dose-and time-dependent manner,which was attributable to the upregulation of Nav1.3 *** VGSC current density and Nav1.3 expression were significantly abolished by preincubation with inhibitors of mitogen-activated protein kinase kinase(PD98059),p38 mitogen-activated protein kinase(SB203580),and Jun NH2-terminal kinase 1/2 inhibitor(SP600125).Interestingly,TGF-β1 significantly increased the firing threshold of action potentials but did not change their firing rate in cortical *** findings suggest that TGF-β1 can increase Nav1.3 expression through activation of the ERK1/2-JNK-MAPK pathway,which leads to a decrease in the firing threshold of action potentials in cortical neurons under pathological ***,this contributes to the occurrence and progression of neuroexcitatory-related diseases of the central nervous system.

关键词： central nervous system cortical neurons ERK firing properties JNK Nav1.3 p38 transforming growth factor-beta 1 traumatic brain injury voltage-gated sodium currents

PDH到622Mb/s SDH/SONET映射芯片实现

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电子学报 2010年第8期38卷 1945-1951页

作者：叶波李天望张立军罗敏上海电力学院计算机与信息工程学院上海200090 武汉大学集成电路与通讯软件系湖北武汉430079 苏州大学电子信息学院江苏苏州215021 朗讯科技光网络有限公司上海200233

设计了PDH到622 Mb/s SDH/SONET的映射及逆映射芯片.集成了DS1/E1/J1成帧器、DS1/DS3复接电路和E1/E3复接电路,具有622 Mb/s和155 Mb/s的高速标准接口和3通道STM-1/STS-3分插复用总线接口,支持复用段1+1保护和UPSR环形网络拓扑结构.单... 详细信息

设计了PDH到622 Mb/s SDH/SONET的映射及逆映射芯片.集成了DS1/E1/J1成帧器、DS1/DS3复接电路和E1/E3复接电路,具有622 Mb/s和155 Mb/s的高速标准接口和3通道STM-1/STS-3分插复用总线接口,支持复用段1+1保护和UPSR环形网络拓扑结构.单片实现84通道DS1/J1或63通道E1到STM-1/STS-3的映射复用功能及多通道DS3/E3/STS-1到STM-4/STS-12的映射复用功能.支持点对点应用和环形应用,交换模式支持2016通道DS0/E0的应用.4颗芯片实现336通道DS1/J1或252通道E1到STM-4/STS-12的映射复用功能.采用TSMC 0.13μm CMOS工艺流片,芯片规模约600万门,700管脚PGBA封装,满足光纤通信传输的要求,并成功用于光纤通信设备.

关键词： SDH/SONET 映射器芯片实现 UPSR

Multivariate synergy for heightening ionic HOF robustness and proton conductivity

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Science China Chemistry 2025年

作者： Xiang-tian Bai li-Hui Cao Xu-Yong Chen Jia-Yu wang Zi-Ye Zhou Shaanxi Key Laboratory of Chemical Additives for Industry College of Chemistry and Chemical EngineeringShaanxi University of Science and Technology

Multicomponent crystalline materials construct more complex and diversified structures with different combinations and modes,which expands the material structure space and application *** this work,we have successfully synthesized two twocomponent ionic hydrogen-bonded organic frameworks （iHOF-32 and iHOF-33） and a three-component iHOF （iHOF-34）.Guanidine hydrochloride which is rich in N and H and has a short chain length strengthens the hydrogen bonding network of iHOF,thereby aiding in the efficient migration of *** rigid architecture of 1,1'-diamino-4,4'-bipyridine diiodide furnishes additional scaffolding for the iHOFs and enhances its robustness under harsh ***-34 accomplishes the combination of the ultra-high proton conductivity of iHOF-32 and the robustness of iHOF-33 by the synergistic interaction among the three components of *** addition,iHOF-34 was fabricated as a composite membrane with a peak proton conductivity of 1.55×10-1S cm-1,which is 3.8 times higher than that of recast Nafion,and has extensive potential in fuel *** self-assembly strategy of multicomponent HOFs opens up new avenues for constructing diverse structures while promising new opportunities for applications in the fields of proton conduction and energy storage.

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Construction of a lentiviral vector for RNA interference of human VIM gene and its silencing effect in pancreatic cancer cells

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The Chinese-German Journal of Clinical Oncology 2009年第3期8卷 145-149页

作者： Jianxin Jiang Ming Shen Renyi Qin Rui tian Jing li Min wang Department of Biliary-Pancreatic Surgery Tongji Hospital Tongji Medical College Huazhong University of Scienceand Technology Wuhan 430030 China

Objective： To construct a lentiviral expression vector for RNA interference （RNAi） of human VIM gene; and assess its gene silencing effect in pancreatic cancer cell line Panc-1. Methods： Three pairs of human VIM gene short hairpin RNA（shRNA） sequences were designed using a software available on-line and one pair came from document. After synthesis and annealing, four double-stranded oligonucleotides （dsOligo） were cloned into the pGCL-GFP/U6 plasmid, which were subsequently confirmed by polymerase chain reaction （PCR） and DNA sequencing analysis. Real-time PCR and Westemblotting were used to screen the effective pGCL-GFP-shRNA plasmid in 293T cells, then the most effective one was packed into the recombinant lentivirus Lv-VIM-shRNA with lentiviral packing materials pHelper 1.0 and pHelper 2.0 in 293T cells. The titer of lentivirus was determined by hole-by-dilution titer assay. The silencing effect of Lv-VIM-shRNA in Panc-1 calls were validated by real-time PCR and Western-blotting. Results： An effective Lv-VIM-shRNA was successfully constructed. The titer of lentivirus was determined on 2× 10^9TU/mL. The expressions of VIM mRNA and vimentin were down-regluated in the Panc-1 cells infected with Lv-VIM-shRNA. Conclusion： An effective Lv-VIM-shRNA could inhibit the expression of VIM gene in Panc-1 cells in vitro, which provides a tool for investigating the role of VIM gene in the signaling pathway involved in tumorigenesis and progression of pancreatic cancer and searching new therapeutic targets.

关键词： vimentin RNA interference lentivirus pancreatic carcinoma

Influence of sinomenine on protein profiles of peripheral blood mononuclear cells from ankylosing spondylitis patients： a pharmacoproteomics study

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Chinese Medical Journal 2013年第19期126卷 3645-3650页

作者： HUANG Zhi-xiang TAN Jin-hui li tian-wang DENG Wei-ming QIU Ke-wei liAO Ze-tao ZENG Zhao-qiu Department of Rheumatology and Immunology Guangdong No. 2Provincial People's Hospital Guangzhou Guangdong 510317 China Department of Rheumatology Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510630 China

Background Ankylosing spondylitis （AS） is a common inflammatory rheumatic disease which lacks satisfactory treatment so *** （SIN） is an alkaloid and has recently been utilized in treating multiple rheumatic diseases including AS in China,but its exact mechanism remains to be *** study investigated the alteration of proteome in peripheral blood mononuclear cells （PBMCs） from AS *** Thirty AS patients were enrolled in this *** from each AS patient were cultured in medium with or without SIN *** PBMCs proteins from both groups were separated by two-dimensional electrophoresis （2-DE） and analyzed by mass spectrometry （MS）.Two differentially expressed proteins were then chosen to be verified using Western *** Seven proteins,including α-synuclein （SNCA）,calmodulin （CALM）,acidic leucine-rich nuclear phosphoprotein 32 family member A （ANP32A）,chloride intracellular channel protein 1 （CliC1）,guanine nucleotide-binding protein G（I）/G（S）/ G（T） subunit beta-1 （GNB1）,gelsolin （GSN） and histone H2B type 1-M （HISTH2BM）were over-expressed,while coronin1A （CORO1A） was under-expressed in the SIN-treated *** bioinformatics search indicated that the changes of SNCA,ANP32A and CliC1 pertained to apoptosis,while changes of GSN and CORO1A were associated with both apoptosis and inhibition of immunological *** GSN and CORO1A were selected to validate by Western blotting and the results were consistent with those of *** There were 8 differentially expressed proteins in the SIN-treated PBMCs,which might shed some light on the mechanism of SIN in the treatment of AS.

关键词： ankylosing spondylitis sinomenine peripheral blood mononuclear cells pharmacoproteomics

Screening disease-associated proteins from sera of patients with rheumatoid arthritis： a comparative proteomic study

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Chinese Medical Journal 2010年第5期123卷 537-543页

作者： li tian-wang ZHENG Ben-rong HUANG Zhi-xiang liN Qu ZHAO li-ke liAO Ze-tao ZHAO Ji-jun liN Zhi-ming GU Jie-ruo Department of Rheumatology Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510630 China Department of Rheumatology Beijing Hospital Beijing 100730 China

Background Rheumatoid arthritis （RA） is an autoimmune disease characterized by chronic inflammation at the synovial membrane. Although great progress has been made recently in exploring the etiology and pathogenesis of RA, its molecular pathological mechanism remains to be further defined and it is still a great challenge in determining the diagnosis and in choosing the appropriate therapy in early patients. This study was performed to screen candidate RA-associated serum proteins by comparative proteomics to provide research clues to early diagnosis and treatment of RA. Methods Sera isolated from 6 RA patients and 6 healthy volunteers were pooled respectively and high-abundance proteins were depleted by Plasma 7 Multiple Affinity Removal System. The protein expression profiles between the two groups were then compared by two-dimensional gel electrophoresis （2-DE） and the proteins over/under-expressed by more than 3-fold were identified by mass spectrometry analysis. To validate the differential expression levels of the identified proteins between the two groups, EliSA was performed in two of the identified proteins in individual sera from 32 RA patients and 32 volunteers. Results Eight proteins which over/under-expressed in sera of RA patients were identified. Among them, chain A of transthyretin （TTR） was under-expressed, while serum amyloid A protein, apolipoprotein A （ApoA）-IV, ApoA-IV precursor, haptoglobin 2, ceruloplasmin （Cp）, immunoglobulin superfamily 22 and HT016 were over-expressed. EliSA test confirmed that Cp expressed remarkably higher while TTR obviously lower in RA group compared with volunteer group. Conclusion There were 8 identified proteins differentially expressed between RA group and volunteer group, which might be candidate RA-associated proteins and might be promising diagnostic indicators or therapeutic targets for RA.

关键词： rheumatoid arthritis serum proteomics gel electrophoresis, two-dimensional

人文社会科学领域大数据研究趋势测度:国内外比较

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自然辩证法研究 2018年第4期34卷 65-71页

作者：李天柱王倩辽宁科技大学工商管理学院辽宁鞍山114051

大数据已经成为国内外科学界关注的热点。为全面刻画国内外人文社会科学领域大数据研究的动态景观,论文基于SSCI、CSSCI来源的相关文献数据,采用科学计量学的方法及指标、时间序列模型、共词分析和数据可视化研究方法对其开展趋势测度,... 详细信息

大数据已经成为国内外科学界关注的热点。为全面刻画国内外人文社会科学领域大数据研究的动态景观,论文基于SSCI、CSSCI来源的相关文献数据,采用科学计量学的方法及指标、时间序列模型、共词分析和数据可视化研究方法对其开展趋势测度,并在此基础上揭示近年来国内外人文社会科学领域有关大数据的最新研究现状、研究热点、前沿主题及发展动向,为未来人文社会科学领域的大数据研究提供借鉴。

关键词：大数据人文社会科学趋势测度国内外比较科学计量学

Dip2a regulates stress susceptibility in the basolateral amygdala

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Neural Regeneration Research 2025年第6期20卷 1735-1748页

作者： Jing li Zixuan He Weitai Chai Meng tian Huali Yu Xiaoxiao He Xiaojuan Zhu Key Laboratory of Molecular Epigenetics Ministry of Education Institute of Genetics and CytologyNortheast Normal UniversityChangchunJilin ProvinceChina

Dysregulation of neurotransmitter metabolism in the central nervous system contributes to mood disorders such as depression, anxiety, and post–traumatic stress disorder. Monoamines and amino acids are important types of neurotransmitters. Our previous results have shown that disco-interacting protein 2 homolog A(Dip2a) knockout mice exhibit brain development disorders and abnormal amino acid metabolism in serum. This suggests that DIP2A is involved in the metabolism of amino acid–associated neurotransmitters. Therefore, we performed targeted neurotransmitter metabolomics analysis and found that Dip2a deficiency caused abnormal metabolism of tryptophan and thyroxine in the basolateral amygdala and medial prefrontal cortex. In addition, acute restraint stress induced a decrease in 5-hydroxytryptamine in the basolateral amygdala. Additionally, Dip2a was abundantly expressed in excitatory neurons of the basolateral amygdala, and deletion of Dip2a in these neurons resulted in hopelessness-like behavior in the tail suspension test. Altogether, these findings demonstrate that DIP2A in the basolateral amygdala may be involved in the regulation of stress susceptibility. This provides critical evidence implicating a role of DIP2A in affective disorders.

关键词： 5-hydroxytryptamine acute restraint stress basolateral amygdala CaMKII neurons DIP2A metabolomics neurotransmitters principal component analysis stress susceptibility tryptophan