Screening disease-associated proteins from sera of patients with rheumatoid arthritis： a comparative proteomic study
Screening disease-associated proteins from sera of patients with rheumatoid arthritis： a comparative proteomic study作者机构：Department of Rheumatology Third Affiliated Hospital of Sun Yat-sen University Guangzhou Guangdong 510630 China Department of Rheumatology Beijing Hospital Beijing 100730 China
出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)
年 卷 期：2010年第123卷第5期
页 面：537-543页
核心收录：
学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 100704[医学-药物分析学] 100705[医学-微生物与生化药学] 1002[医学-临床医学] 07[理学] 071005[理学-微生物学] 10[医学]
基 金：This study was partly supported by grants from National Natural Science Foundation of China (No. 30325019 and 30471611) Natural Science Foundation of Guangdong Province (No. 05001703) and Science and Technology Project of Guangdong Province (No. 2008B030301039).Acknowledgments: We thank the patients and healthy volunteers who participated in this study and also thank Shanghai Applied Protein Technology Co. Ltd for performing the spectrometry analysis and providing technical support for the 2-DE experiment
主 题：rheumatoid arthritis serum proteomics gel electrophoresis, two-dimensional
摘 要：Background Rheumatoid arthritis （RA） is an autoimmune disease characterized by chronic inflammation at the synovial membrane. Although great progress has been made recently in exploring the etiology and pathogenesis of RA, its molecular pathological mechanism remains to be further defined and it is still a great challenge in determining the diagnosis and in choosing the appropriate therapy in early patients. This study was performed to screen candidate RA-associated serum proteins by comparative proteomics to provide research clues to early diagnosis and treatment of RA. Methods Sera isolated from 6 RA patients and 6 healthy volunteers were pooled respectively and high-abundance proteins were depleted by Plasma 7 Multiple Affinity Removal System. The protein expression profiles between the two groups were then compared by two-dimensional gel electrophoresis （2-DE） and the proteins over/under-expressed by more than 3-fold were identified by mass spectrometry analysis. To validate the differential expression levels of the identified proteins between the two groups, ELISA was performed in two of the identified proteins in individual sera from 32 RA patients and 32 volunteers. Results Eight proteins which over/under-expressed in sera of RA patients were identified. Among them, chain A of transthyretin （TTR） was under-expressed, while serum amyloid A protein, apolipoprotein A （ApoA）-IV, ApoA-IV precursor, haptoglobin 2, ceruloplasmin （Cp）, immunoglobulin superfamily 22 and HT016 were over-expressed. ELISA test confirmed that Cp expressed remarkably higher while TTR obviously lower in RA group compared with volunteer group. Conclusion There were 8 identified proteins differentially expressed between RA group and volunteer group, which might be candidate RA-associated proteins and might be promising diagnostic indicators or therapeutic targets for RA.
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