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民国时期乡村教师的身份认同与社会功能——以江南地区为考察中心

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浙江师范大学学报（社会科学版） 2017年第6期42卷 106-110页

作者：周晓燕李嘉君浙江师范大学教师教育学院浙江金华 321004

乡村教师在推动乡村社会发展中扮演着重要的角色。在民国这一特殊时期,江南地区的乡村教师彰显着自身的时代特征,他们在面对生活和教学的身份认同困境中做出了一系列的努力,在启迪民众智识、改造乡村社会、推动国家进步方面发挥着特殊... 详细信息

乡村教师在推动乡村社会发展中扮演着重要的角色。在民国这一特殊时期,江南地区的乡村教师彰显着自身的时代特征,他们在面对生活和教学的身份认同困境中做出了一系列的努力,在启迪民众智识、改造乡村社会、推动国家进步方面发挥着特殊的社会功能。

关键词：民国时期身份认同乡村教师社会功能

An inter-laboratory study of DNA-based identity,parentage and species testing in animal forensic genetics

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Forensic Sciences Research 2022年第4期7卷 708-713页

作者： Sreetharan Kanthaswamy Torsten Brendel Luis Cancela Denise A.Andrade de Oliveira Bertram Brenig Carmen Cons Julian A.Crespi Markéta Dajbychová Andreas Feldl Tomohito Itoh Vincenzo Landi Amparo Martinez Malgorzata Natonek-Wisniewska Robert F.Oldt Anna Radko Oscar Ramírez Clementina Rodellar Manuel Ruiz-Girón David Schikorski María Elena Turba Guillermo Giovambatista School of Mathematics and Natural Sciences Arizona State University(ASU)at the West CampusGlendaleAZUSA California National Primate Research Center University of CaliforniaDavisCAUSA Evolutionary Biology Graduate Program School of Life SciencesArizona State UniversityTempeAZUSA Eurofins Genomics Europe Applied Genomics GmbH EbersbergGermany IDENTItaS MontevideoUruguay Laboratório de Genética Escola de Veterinária da UFMGBelo HorizonteBrazil Institute of Veterinary Medicine University of GoettingenGoettingenGermany Facultad de Veterinaria Laboratorio de Genética Bioquímica(LAGENBIO)Instituto Agroalimentario de Aragón-IA2(Universidad de Zaragoza-CITA)ZaragozaSpain Facultad de Ciencias Veterinarias UNLP IGEVET-Instituto de Genética Veterinaria(UNLP-CONICET LAPLATA)La PlataArgentina Genomia s.r.o. PlzeňCzech Republic Maebashi Institute of Animal Science Livestock Improvement Association of Japan(LIAJ)MaebashiJapan Department of Veterinary Medicine Animal Breeding and GeneticsUniversity of Bari“Aldo Moro”ValenzanoItaly Department of Genetics Faculty of Veterinary SciencesUniversity of CórdobaCórdobaSpain Department of Animal Molecular Biology National Research Institute of Animal ProductionBalicePoland Vetgenomics SL BarcelonaSpain Laboratorio de Biología Molecular y Genómica Hispalis Biolab S.L.U.SevilleSpain Laboratoire LABOFARM-GENINDEXE LOUDEACCedexFrance Genefast SRL ForlìItaly

The probative value of animal forensic genetic evidence relies on laboratory accuracy and ***-laboratory comparisons allow laboratories to evaluate their performance on specific tests and analyses and to continue to monitor their *** International Society for Animal Genetics(ISAG)administered animal forensic comparison tests(AFCTs)in 2016 and 2018 to assess the limitations and capabilities of laboratories offering forensic identification,parentage and species determination *** AFCTs revealed that analyses of low DNA template concentrations(≤300 pg/μL)constitute a significant challenge that has prevented many laboratories from reporting correct identification and parentage ***,a lack of familiarity with species testing protocols,interpretation guidelines and representative databases prevented over a quarter of the participating laboratories from submitting correct species determination *** laboratories showed improvement in their genotyping accuracy over ***,the use of forensically validated standards,such as a standard forensic short tandem repeat(STR)kit,preferably with an allelic ladder,and stricter guidelines for STR typing,may have prevented some common issues from occurring,such as genotyping inaccuracies,missing data,elevated stutter products and loading *** AFCTs underscore the importance of conducting routine forensic comparison tests to allow laboratories to compare results from each *** should keep improving their scientific and technical capabilities and continuously evaluate their personnel’s proficiency in critical techniques such as low copy number(LCN)analysis and species *** this is the first time that the ISAG has conducted comparison tests for forensic testing,findings from these AFCTs may serve as the foundation for continuous improvements of the overall quality of animal forensic genetic testing.

关键词： Forensic sciences forensic genetics comparison test forensic DNA analysis individual identification parentage test species test

Immunoprofiling of Drosophila Hemocytes by Single-cell Mass Cytometry

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Genomics, Proteomics & Bioinformatics 2021年第2期19卷 243-252页

作者： Jozsef A.Balog Viktor Honti Eva Kurucz Beata Kari Laszlo G.Puskas Istvan Ando Gabor J.Szebeni Laboratory of Functional Genomics Institute of GeneticsBiological Research CentreSzeged H-6726Hungary University of Szeged Ph.D.School in BiologySzeged H-6726Hungary Immunology Unit Institute of GeneticsBiological Research CentreSzeged H-6726Hungary Department of Physiology Anatomy and NeuroscienceFaculty of Science and InformaticsUniversity of SzegedSzeged H-6726Hungary

Single-cell mass cytometry(SCMC)combines features of traditional flow cytometry(i.e.,fluorescence-activated cell sorting)with mass spectrometry,making it possible to measure several parameters at the single-cell level for a complex analysis of biological regulatory *** this study,we optimized SCMC to analyze hemocytes of the Drosophila innate immune *** used metal-conjugated antibodies(against cell surface antigens H2,H3,H18,L1,L4,and P1,and intracellular antigens 3A5 and L2)and anti-IgM(against cell surface antigen L6)to detect the levels of antigens,while anti-GFP was used to detect crystal cells in the immune-induced *** investigated the antigen expression profile of single cells and hemocyte populations in naive states,in immune-induced states,in tumorous mutants bearing a driver mutation in the Drosophila homologue of Janus kinase(hopTum)and carrying a deficiency of the tumor suppressor gene lethal(3)malignant blood neoplasm-1[l(3)mbn1],as well as in stem cell maintenance-defective hdcD84 mutant *** analysis enabled the discrimination of the functionally different major hemocyte subsets for lamellocytes,plasmatocytes,and crystal cells,and delineated the unique immunophenotype of Drosophila *** have identified subpopulations of L2^(+)/P1^(+)and L2^(+)/L4^(+)/P1^(+)transitional phenotype cells in the tumorous strains l(3)mbn1 and hopTum,respectively,and a subpopulation of L4^(+)/P1^(+)cells upon immune *** results demonstrated for the first time that SCMC,combined with multidimensional bioinformatic analysis,represents a versatile and powerful tool to deeply analyze the regulation of cell-mediated immunity of Drosophila.

关键词： Mass cytometry Innate immunity Drosophila Single-cell analysis Hemocyte

硷原子主系线旁之吸收光带:陈氏解释之检讨(英文)

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中国物理学报 1945年 17-20页

作者：吴大猷国立北京大学物理学系

崄原子主系线旁,在参有He,Ne,Ar,N2等气体时,有连续吸收光带发生。此等光带,昔Kuhn-Oldenburg二氏有理论解释之。近陈尚义氏对此有新解释。本文乃检讨陈氏之理论,指出其困难点六项。结论:Kuhn-Oldenburg之理论,仍为此现象之最满意的解释。

关键词：光带系线原子分子

Study of the Firing Temperature of Marajoara Archaeological Ceramics by EPR Associated to INAA Data

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Journal of Environmental Science and Engineering(A) 2012年第9期1卷 1108-1116页

作者： Kelly P. Nunes Casimiro S. Munita Rosimeiri G. Toyota Paulo T.M. Oliveira Nilo F. Cano Denise Schaan Oswaldo Baffa Luiz C. Oliveira Carlos A. Brunello Eduardo G. Neves Ce1ia C. Demartini Nuclear and Energy Research Institute University of Sao Paulo Sao Paulo 5508-000 Brazil Department of Anthropology Basic Campus 1 Federal University of Par6 Beldm 66075-110 Brazil Magnetic Resonance Laboratory Department of Physics University of Sao Paulo Ribeirao Preto 14040-901 Brazil Archaeology and Ethnology Museum Department of Archaeology University of Sao Paulo Sao Paulo 05508-900 Brazil

The Marajó Island is the largest island of the Marajō archipelago, located in the north of Brazil. In this region, at the end of 19th century, it was identified an archaeological society named Marajora, which occupied the island from 400 to 1,350 AD. It was characterized by a ceramic style recognized by its beauty and exuberance. Until this moment, the physical and chemical analysis studies in this ceramics are scarce and this kind of studies may help archaeological studies performed in the island. With this purpose, this work presents a preliminary study of the firing temperature in the ceramics. For that, the elementary concentrations of 109 samples from Museu de Archaeology and Ethnology Museum of the University of Sáo Paulo and samples collected in the Marajá Island were studied. The samples were analyzed using instrumental neutron activation analysis in which the elemental concentration of Ce, Co, Cr, Cs, Eu, Fe, Hf, K, La, Lu, Na, Nd, Rb, Sc, Sm, ta, Tb, Th, U, Yb and Zn were determined. The interpretation of the results was made using multivariate statistical approaches and showed the existence of three chemical groups. Some samples of each cluster were chosen for the determination of their firing temperatures by electron paramagnetic resonance, in order to verify if different firing temperatures was associated with different elemental groups of ceramic samples. The results showed that there are no statistical differences in the firing temperature between the samples from Archaeology and Ethnology Museum and the contemporary samples of the Marajá Island.

关键词： Maraj6 island archaeological ceramics INAA EPR multivariate statistical analysis.

冬青素诱导宫颈癌HeLa细胞凋亡及其机制初步研究

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中华中医药杂志 2014年第5期29卷 1550-1553页

作者：程齐来李洪亮李映辰刘塔斯黄志勤湖南中医药大学药学院长沙410208 赣南医学院药学院赣州341000

目的:研究冬青素(ia)对人宫颈癌HeLa细胞凋亡的诱导作用及其相关机制。方法:建立人宫颈癌HeLa细胞体外培养体系,采用CCK-8法检测细胞活力,MTT法测定乳酸脱氢酶(LDH),免疫比色法检测细胞增殖情况,DAPI荧光染色观察细胞凋亡形态学变化,Wes... 详细信息

目的:研究冬青素(ia)对人宫颈癌HeLa细胞凋亡的诱导作用及其相关机制。方法:建立人宫颈癌HeLa细胞体外培养体系,采用CCK-8法检测细胞活力,MTT法测定乳酸脱氢酶(LDH),免疫比色法检测细胞增殖情况,DAPI荧光染色观察细胞凋亡形态学变化,Western Blot检测Caspase-3/7和NF-κB蛋白表达水平。结果:用冬青素处理过的HeLa细胞,LDH释放增加,呈剂量和时间依赖性。在CCK-8和5-溴脱氧尿嘧啶核苷(BrdU)测定过程中,冬青素比标准药物抗HeLa活性更强,随浓度和作用时间的增加,细胞的活性不断下降,对HeLa增殖的抑制作用明显。DAPI染色观察用冬青素处理过的HeLa细胞浓缩明显,细胞核分散,染色质凝聚。Western Blot结果显示用冬青素处理过的HeLa细胞Caspase-3/7的活性显著增加(P<0.01),NF-κB蛋白表达水平下降(P<0.01)。结论:冬青素具有诱导HeLa细胞凋亡的作用,其机制可能与激活Caspase-3/7和抑制NF-κB蛋白表达有关。

关键词：冬青素 HeLa细胞凋亡 NF-κB

冀东分水岭岩体锆石U-Pb年代学、地球化学特征及地质意义

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地质学报 2018年第9期92卷 1904-1917页

作者：李玉静王玉芳徐国新李智斌齐云飞孙金龙赵丙刚张勃越悦河北省地质学会石家庄050051 河北省地矿局第八地质大队河北秦皇岛066001

冀东分水岭岩体位于马兰峪隆起西段,为复式岩体,其岩性为花岗闪长岩和黑云母二长花岗岩,花岗闪长岩呈带状环绕黑云母二长花岗岩分布,锆石U-Pb定年揭示其年龄分别为157.4±2.7Ma和162.0±4.0Ma。花岗闪长岩的SiO_264.50%~65.56%,K_2O+Na_... 详细信息

冀东分水岭岩体位于马兰峪隆起西段,为复式岩体,其岩性为花岗闪长岩和黑云母二长花岗岩,花岗闪长岩呈带状环绕黑云母二长花岗岩分布,锆石U-Pb定年揭示其年龄分别为157.4±2.7Ma和162.0±4.0Ma。花岗闪长岩的SiO_264.50%~65.56%,K_2O+Na_2O 7.24%~7.70%,K_2O/Na_2O 0.58~0.67,A/CNK0.91~0.97,呈高硅富钾,属准铝质高钾钙碱性系列,为I型花岗岩;而黑云母二长花岗岩SiO_273.25%~74.56%,K_2O+Na_2O 7.22%~8.58%,K_2O/Na_2O 0.86~1.04,A/CNK 1.09~1.24,较花岗闪长岩更富硅、钾,为过铝质高钾钙碱性系列,属成熟度不高的A型花岗岩。两者稀土配分曲线呈右倾型式,具稀土总量较低,轻重稀土分馏明显,微量元素均亏损Nb-ta、Zr-Hf,显示以壳源成分为主,具幔源混染特征。花岗闪长岩具弱Eu正异常,且富集Sr,源区深度较大;而黑云母二长花岗岩具Eu负异常,亏损Sr,源区有少量斜长石残留。岩体形成与太平洋板块俯冲构造背景密切相关,为中生代构造转换体制的产物。

关键词：分水岭复式岩体 U-Pb锆石年代学地球化学特征岩浆起源构造背景

NKG2D和NKG2A及相应配体MHC-ia/B和HLA-E在急性白血病的表达及意义

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中国实验血液学杂志 2011年第2期19卷 312-316页

作者：葛淑静段连宁罗渊索塔林陆承荣唐捷空军总医院(医务部中心实验室)北京100142 河北北方学院研究生部河北张家口075000 中国科学院生物物理研究所北京100101

本研究旨在初步探讨NK细胞受体NKG2D和NKG2A在初发ALL和AML患者NK细胞、CD3+T细胞的表达其及相应配体MHC-I A/B和HLA-E在白血病细胞表达差异性和免疫学意义。用流式细胞术检测NK92细胞对8种白血病细胞系的杀伤效率,并检测60例初发急性... 详细信息

本研究旨在初步探讨NK细胞受体NKG2D和NKG2A在初发ALL和AML患者NK细胞、CD3+T细胞的表达其及相应配体MHC-I A/B和HLA-E在白血病细胞表达差异性和免疫学意义。用流式细胞术检测NK92细胞对8种白血病细胞系的杀伤效率,并检测60例初发急性白血病患者(ALL和AML各30例)骨髓中NKG2D和NKG2A在NK和CD3+T细胞的表达及其相应配体MHC-I A/B和HLA-E在白血病细胞的表达。结果表明,NK92对不同白血病细胞系的杀伤效率存在差异。ALL患者NK细胞和CD3+T细胞的NKG2D及NKG2A阳性表达率与AML患者相比均无显著差异(p>0.05),但是ALL患者白血病细胞MHC-I A/B和HLA-E阳性表达率均明显高于AML患者(p<0.05)。结论:ALL与AML患者免疫细胞功能的差异性可能与白血病细胞配体表达的不同有关,而与本身NK及T细胞表达的杀伤和抑制性受体无关。

关键词：急性白血病 NKG2D NKG2A MHC-ia/B HLA-E

心肌特异性丝裂原活化蛋白激酶激活的蛋白激酶2基因敲除对小鼠心肌缺血/再灌注损伤的影响及其机制

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上海医学 2023年第4期46卷 201-207页

作者：孙丽爽于洋陈苗塔方欣冯艳红锦州医科大学附属第三医院超声科辽宁锦州121000 锦州医科大学附属第一医院超声科

目的研究心肌特异性丝裂原活化蛋白激酶激活的蛋白激酶2(MK2)基因敲除对小鼠心肌缺血/再灌注(I/R)损伤的影响及其机制。方法将野生型C57BL/6J小鼠随机分为MK2^(+/+)组、MK2^(+/+)+I/R组,心肌特异性MK 2基因敲除的C57BL/6J小鼠随机分为MK... 详细信息

目的研究心肌特异性丝裂原活化蛋白激酶激活的蛋白激酶2(MK2)基因敲除对小鼠心肌缺血/再灌注(I/R)损伤的影响及其机制。方法将野生型C57BL/6J小鼠随机分为MK2^(+/+)组、MK2^(+/+)+I/R组,心肌特异性MK 2基因敲除的C57BL/6J小鼠随机分为MK2^(-/-)组、MK2^(-/-)+I/R组,每组12只。采用左冠状动脉前降支夹闭的方法建立小鼠心肌I/R损伤模型。比较各组小鼠心肌中MK2、磷酸化MK2(p-MK2)蛋白质相对表达量,心功能超声指标[左心室射血分数(LVEF)、左心室短轴缩短分数(LVFS)),血清心肌损伤标志物[心肌肌钙蛋白I(cTnI)、磷酸肌酸激酶同工酶(CK-MB)水平],心肌组织结构、心肌梗死面积[梗死区域(ia)面积/左心室(LV)面积],心肌细胞凋亡情况[心肌细胞凋亡率、裂解型含半胱氨酸的天冬氨酸蛋白水解酶-3(cleaved caspase-3)相对表达量],心肌组织炎症反应指标[NF-κB相对表达量及TNF-α、细胞间黏附分子-1(ICAM-1)含量]和氧化应激反应指标[活性氧(ROS)、超氧化物歧化酶(SOD)、丙二醛(MDA)含量]。结果MK2^(+/+)组与MK2^(+/+)+I/R组间小鼠心肌中MK2蛋白质相对表达量的差异无统计学意义(P>0.05),MK2^(+/+)+I/R组小鼠心肌中p-MK2蛋白质相对表达量显著高于MK2^(+/+)组(Pia/LV值显著低于MK2^(+/+)+I/R组(P<0.05)。与MK2^(+/+)组比较,MK2^(+/+)+I/R组小鼠的心肌细胞凋亡率,心肌中leaved caspase-3、NF-κB蛋白质相对表达量及TNF-α、ICAM-1含量,以及心肌中ROS、MDA含量均显著增高(P值均<0.05),SOD含量显著降低(P<0.05);与MK2^(+/+)+I/R组小鼠比较,MK2^(-/-)+I/R组小鼠的心肌细胞凋亡率,心肌中cleaved caspase-3、NF-κB相对表达量及TNF-α、ICAM-1含量,以及心肌中ROS、MDA含量均显著降低(P值均<0.05),SOD含量显著增高(P<0.05)。结论MK2参与心肌I/R损伤的发生,敲除MK 2基因可显著减轻心肌I/R损伤,并减少细胞凋亡,减轻炎症反应和氧化应激反应。

关键词：心肌缺血/再灌注损伤丝裂原活化蛋白激酶激活的蛋白激酶2 基因敲除细胞凋亡炎症反应氧化应激反应