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Apoferritin nanoparticle based dual-antigen influenza conjugate vaccine with potential cross-protective efficacy against heterosubtypic influenza virus

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Particuology 2022年第5期20卷 56-64页

作者： Yanan Sheng Jiangxue Wei Zhengjun Li Zhiguo Su Guanghui Ma songping zhang State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China University of Chinese Academy of Sciences Beijing 100049China

Ferritin nanoparticles with self-assembling properties have been widely explored as vaccine carrier by displaying foreign antigens through genetic fusion *** the present work,an apoferritin(AFt)nanoparticle was tested as influenza vaccine carrier by chemically conjugating a matrix protein 2 ectodomain(M2e)antigen peptide or/and the full-length hemagglutinin(HA)antigen on the outer surface of the AFt,with heterobifunctional sSMCC or SM(PEG)_(24) containing PEG chain as *** each AFt nanoparticle,about 30-32 M2e or 1.8 HA antigen could be *** AFt-(PEG)24-M2e,in which the M2e was coupled through SM(PEG)_(24) containing PEG chain,conferred higher protective efficacy in immunized mice than AFt-M2e did,but was less effective than AFt-(PEG)_(24)-*** both M2e and HA were coupled,the synthesized dual-antigen vaccine candidate AFt-(PEG)_(24)-M2e/HA elicited high level of M2e and HA antigen-specific antibodies and conferred 100%protection against lethal infection of homologous PR8 HI N1 virus strain and 70%protection against a heterologous A/FM/1/47(FM1,H1N1)strain,which was more effective than the M2e or HA single antigen vaccine *** potential cross-protective effect of the dual-antigen vaccine was further demonstrated by significant specific hemagglutination inhibition(HAI)titers in serum of the immunized mice against three other heterologous viral strains including A/Singapore/GPl908/2015(IVR-180)H1N1,A/Anhui/1/2005 H5N1,and A/Hong Kong H3N2.

关键词： Influenza vaccine Apoferritin nanoparticle Hemagglutinin M2e Conjugate vaccine

The potential of ionic liquids in biopharmaceutical engineering

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Chinese Journal of Chemical Engineering 2021年第2期34卷 236-243页

作者： Xuan Lin Zhiguo Su Yanli Yang songping zhang State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing 100190China

Biopharmaceuticals,such as proteins,peptides,nucleic acids and vaccines,bring about great hopes for the prevention and treatment of various diseases,but the industrialization of these products still faces challenges such as structural instability,inefficient bioactivity and low *** liquids(ILs),the marvelous solvent media with inimitable and tunable properties,may provide alternative solutions to overcome the above problems of biopharmaceutical *** has gradually been made through studies by combination of ILs with *** applications involved the stabilization,protection,and delivery of *** trends are being forwarded to using ILs in vaccines and nucleic acid ***,challenges remain on the toxicity and safety ***,the cost of adding ILs to the benefits of biopharmaceuticals need to be considered.

关键词： Ionic liquids Biopharmaceutical engineering Biomacromolecules Stabilization Vaccine Delivery

Laccase immobilized on magnetic nanoparticles by dopamine polymerization for 4-chlorophenol removal

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Green Energy & Environment 2017年第4期2卷 393-400页

作者： Di zhang Manfeng Deng Hongbin Cao songping zhang He Zhao Beijing Engineering Research Center of Process Pollution Control Division of Environment Technology and Engineering Institute of Process EngineeringChinese Academy of Sciences Collaborative Innovation Center of Chemical Science and Engineering State Key Laboratory of Biochemical Engineering Institute of Process Engineering Chinese Academy of Sciences

In this work, a new immobilization method based on dopamine(DA) self-polymerization was developed for laccase immobilization on magnetic nanoparticles(Fe_3O_4 NPs). To optimize the immobilization condition including reaction pH, DA concentration and enzyme concentration, a central composite response surface method was applied. The optimal condition was determined as p H value of 5.92, laccase concentration of 0.25 mg mL^(-1) and DA concentration of 12.74 mg mL^(-1), under which a high enzyme activity recovery of 88.17% was *** comparing with free laccase, the stabilities of immobilized laccase towards p H, thermostability, storage were enhanced *** 60% of relative activity for immobilized laccase was remained after being incubated for 6 h at 50℃, but the free laccase only remained 25%. After 40 days of storage at 4℃, the laccase immobilized by DA kept about 89% of its original activity, but the free laccase only retained 48%. After recycled 10 times, the relative activity of immobilized laccase still retained 70%. The immobilized laccase was then applied to catalyze the degradation of 4-chlorophenol(4-CP), 86% percentage of 4-CP was removed within 2 h. After degraded 10 times, the relative activity of immobilized laccase still remained 64% of its initial activity, which exhibits an excellent reusability and operational stability.

关键词： Magnetic nanoparticle Laccase Dopamine Immobilization 4-Chlorophenol removal

Positional assembly of multi-enzyme cascade reaction in polyelectrolyte doped microcapsule through electrospray and layer-by-layer assembly

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Synthetic and Systems Biotechnology 2020年第3期5卷 206-213页

作者： Shiyi Che Jie Wang Xiaoyuan Ji Zhiguo Su Shaomin Wang songping zhang State Key Laboratory of Biochemical Engineering Institute of Process EngineeringChinese Academy of SciencesBeijing100190China University of Chinese Academy of Sciences No.19 Yuquan RoadShijingshan DistrictBeijing100049China College of Chemistry Zhengzhou UniversityZhengzhou450001China

Polyelectrolyte-doped microcapsules(PDM)was fabricated by coaxial electrospray of a mixture of glycerol and water containing 10 mg/mL cationic polyelectrolyte poly(allylamine hydrochloride)(PAH)fed as the core phase solution,and a N,N-dimethylacetylamide solution of 10 wt%polyurethane fed as the shell phase *** system involving Candida Antarctica lipase B(CALB),glucose oxidase(GOD),and horseradish peroxidase(HRP)for cascade reaction was assembled in the PDM at three different places,namely,surface,shell,and *** of enzyme inside aqueous lumen of the PDM was realized by in situ encapsulation through adding the enzyme in the core-phase solution for coaxial *** ion-pairing of enzyme with cationic surfactant CTAB,an organic soluble enzyme-CTAB complex was prepared,so that in situ embedding of enzyme in the shell of the PDM was realized by adding it into the shell phase *** attachment of enzymes was achieved by layer-by-layer(LbL)technology,which is based on the ion-exchange interactions between oppositely charged enzymes and PAH that was doped in *** enzyme-decorated microcapsule was then studied as a microbioreactor,in which 1-Oxododecyla-α-glucopyranoside was converted by CALB to glucose,which was oxidised by GOD to gluconolactone in a second *** hydrogen peroxide produced was then used by HRP to oxidize ABTS to form coloured radical cation ABTS•+for activity *** successful fabrication of the PDM and precise localization of enzymes in the PDM by different strategies were fully *** varying the immobilization strategy,totally six PDM bioreactors with three enzymes precisely positional assembled in different strategies were constructed and their activities for the cascade reaction were investigated and *** PDM micro-bioreactor prepared by novel electrospray technologies provide a smart platform for positional assembly of multi-enzyme cascade reaction in a precise and well-controlle

关键词： Coaxial electrospray Microcapsule Multi-enzyme Cascade reaction Positional assembly Layer-by-layer assembly

Leaf Morphology Genes SRL1 and RENL1 Co-Regulate Cellulose Synthesis and Affect Rice Drought Tolerance

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Rice science 2024年第1期31卷 103-117,I0020-I0022页

作者： LIU Dan ZHAO Huibo WANG Zi’an XU Jing LIU Yiting WANG Jiajia CHEN Minmin LIU Xiong zhang Zhihai CEN Jiangsu ZHU Li HU Jiang REN Deyong GAO Zhenyu DONG Guojun zhang Qiang SHEN Lan LI Qing QIAN Qian HU songping zhang Guangheng State Key Laboratory of Rice Biology and Breeding China National Rice Research InstituteHangzhou 310006China Research Center of Plant Functional Genes and Tissue Culture Technology College of Bioscience and BioengineeringJiangxi Agricultural UniversityNanchang 330045China Qian Qian Academician Workstation National Nanfan Research Institute(Sanya)Chinese Academy of Agricultural SciencesSanya 572024China

The morphological development of rice(Oryza sativa L.)leaves is closely related to plant architecture,physiological activities,and ***,it is unclear whether there is a co-regulatory relationship between the morphological development of leaves and adaptation to drought *** this study,a drought-sensitive,roll-enhanced,and narrow-leaf mutant(renl1)was induced from a semi-rolled leaf mutant(srl1)by ethyl methane sulfonate(EMS),which was obtained from Nipponbare(NPB)through ***-based cloning and functional validation showed that RENL1 encodes a cellulose synthase,allelic to NRL1/*** RENL1 mutation resulted in reduced vascular bundles,vesicular cells,cellulose,and hemicellulose contents in cell walls,diminishing the water-holding capacity of *** addition,the root system of the renl1 mutant was poorly developed and its ability to scavenge reactive oxygen species(ROS)was decreased,leading to an increase in ROS after drought ***,genetic results showed that RENL1 and SRL1 synergistically regulated cell wall *** results revealed a theoretical basis for further elucidating the molecular regulation mechanism of cellulose on rice drought tolerance,and provided a new genetic resource for enhancing the synergistic regulation network of plant type and stress resistance,thereby realizing simultaneous improvement of multiple traits in rice.

关键词： cellulose cell wall drought tolerance leaf morphology rice

2500 t/d熟料生产线窑头电改袋收尘器的实践

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水泥 2022年第2期 37-39页

作者：张松平湖南韶峰南方水泥有限公司湖南湘乡411423

针对2500 t/d熟料生产线原窑头WDJ105型电除尘器,经过长期运行后设备本体故障率高、维护量大、维修成本增加,且不能满足当下高标准环保要求的现状,通过最大限度地利用原电收尘器的壳体、灰斗及风机等基础设施,同时增设了相关降温设施,... 详细信息

针对2500 t/d熟料生产线原窑头WDJ105型电除尘器,经过长期运行后设备本体故障率高、维护量大、维修成本增加,且不能满足当下高标准环保要求的现状,通过最大限度地利用原电收尘器的壳体、灰斗及风机等基础设施,同时增设了相关降温设施,优选除尘排风机,采用耐温性能较高的玻纤覆膜滤袋等,从而实现了电收尘器改为袋收尘器的升级改造,并经过长期的运行跟踪,完全达到了预期的改造目标,取得了良好的经济及社会效益。

关键词：电收尘器袋收尘器玻纤覆膜滤袋排风机排放

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静电耦合亲和层析纯化人血清白蛋白的研究

过程工程学报 2023年第11期23卷 1599-1607页

作者：王思栋王留洋冯雪张松平张万忠罗坚沈阳化工大学制药与生物工程学院辽宁沈阳110142 中国科学院过程工程研究所生化工程国家重点实验室北京100190

人血清白蛋白(Human Serum Albumin,HSA)是人血清中含量最丰富的蛋白,约占血清总蛋白含量的40%~60%。商品化的白蛋白亲和介质(Cibacron Blue F3GA)的配基毒性大、易脱落、载量低,纯化白蛋白回收率较低。本研究制备了一种新型静电耦合亲... 详细信息

人血清白蛋白(Human Serum Albumin,HSA)是人血清中含量最丰富的蛋白,约占血清总蛋白含量的40%~60%。商品化的白蛋白亲和介质(Cibacron Blue F3GA)的配基毒性大、易脱落、载量低,纯化白蛋白回收率较低。本研究制备了一种新型静电耦合亲和介质(DASA-Sepharose,3,5-Diaminobenzoic Acid n-Octyl Succinic Anhydride-Sepharose),并通过一步层析从人血清中高效纯化白蛋白。DASA-Sepharose介质采用3,5-二氨基苯甲酸间隔臂将正辛基琥珀酸酐亲和配体偶联于琼脂糖微球。DASA-Sepharose间隔臂上的羧基通过静电作用吸附白蛋白,进而与正辛基琥珀酸酐亲和配基协同作用实现静电耦合亲和吸附,在保持亲和吸附高特异性的同时大大提高了吸附载量。采用牛血清白蛋白(Bovine Serum Albumin,BSA)为模型蛋白考察了Na Cl浓度和溶液pH对介质静态吸附载量(Qm)的影响,NaCl浓度为0.025~0.06 mol/L时Qm几乎不受影响,而NaCl浓度在0.1 mol/L及以上时Qm显著下降,在pH=5.00的20 mmol/L PBS缓冲体系中,Qm为75.43 mg/mL介质。相比商品化的白蛋白亲和介质Cibacron Blue F3GA的Qm(20 mg/mL介质)提高了约2.7倍。DASA-Sepharose介质能通过一步层析法以较高的收率(94.34%)和纯度(98.20%)从人血清中直接提取白蛋白。圆二色光谱和华法林钠法测定纯化白蛋白的二级结构和小分子药物结合活性,与标准人血清白蛋白基本一致。采用DASA-Sepharose介质可以实现人血清白蛋白的高效分离,本工作为人血清白蛋白的分离纯化提供了新方法。

关键词：人血清白蛋白静电耦合亲和层析 3 5-二氨基苯甲酸正辛基琥珀酸酐间隔臂静电吸附

高效液相尺寸排阻色谱-多角度激光散射定量检测灭活禽流感病毒抗原中完整病毒颗粒

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生物工程学报 2023年第10期39卷 4295-4307页

作者：郝建敏刘幽燕苏志国张松平李正军广西大学化学化工学院广西南宁530004 中国科学院过程工程研究所生化工程国家重点实验室北京100190

本研究旨在建立一种灭活禽流感病毒原料液中完整病毒颗粒准确定量的方法。针对直接采用高效液相尺寸排阻色谱法(high performance size exclusion chromatography,HPSEC)检测灭活禽流感病毒原液存在杂质干扰的问题,首先以H5N8型抗原为对... 详细信息

本研究旨在建立一种灭活禽流感病毒原料液中完整病毒颗粒准确定量的方法。针对直接采用高效液相尺寸排阻色谱法(high performance size exclusion chromatography,HPSEC)检测灭活禽流感病毒原液存在杂质干扰的问题,首先以H5N8型抗原为对象,分别考察了聚乙二醇(polyethylene glycol,PEG)沉淀和离子交换色谱法(ion exchange chromatography,IEC)进行预处理。在优化条件下,经DEAE FF阴离子交换层析纯化预处理,杂蛋白去除率为86.87%,病毒血凝回收率为100%。HPSEC分析预处理后的样品,8.5–10.0 min处色谱峰样品电泳检测显示主要为H5N8病毒蛋白,动态光散射分析平均粒径为127.7nm,推测为完整病毒特征峰;在IEC预处理后的样品中加入抗体进行HPSEC检测,8.5-10.0 min处特征峰消失,显示IEC预处理有效去除了杂质干扰。通过将HPSEC与多角度激光散射技术(multi-angle laser scattering technique,MALLS)联用,可以准确获得样品中完整病毒颗粒的数量,且病毒颗粒数与色谱峰面积具有良好线性关系(R2=0.997)。建立的IEC预处理-HPSEC-MALLS检测方法应用于其他亚型(H7N9)、批次和浓度的病毒原液中完整病毒颗粒数的准确检测,均具有良好适用性,且重复性好,相对标准偏差(relative standard deviation,RSD)<5%,n=3。

关键词：灭活禽流感病毒颗粒定量检测离子交换层析尺寸排阻色谱

多巴胺包埋磁性SiO_2固定化漆酶催化去除4-氯酚

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化工学报 2015年第9期66卷 3705-3711页

作者：张笛邓满凤赵赫曹宏斌张松平中国科学院过程工程研究所北京100190 天津化学化工协同创新中心天津300072

以磁性纳米颗粒为载体,通过多巴胺(dopamine,DA)聚合原位包埋制备了磁性SiO2固定化漆酶(Fe3O4@SiO2-PDA-Lac)。结果显示纳米颗粒尺寸均匀,并且保持较高的饱和磁性。通过最优条件制备出的固定化漆酶在50℃放置6h后,活性保持在63%,而游离... 详细信息

以磁性纳米颗粒为载体,通过多巴胺(dopamine,DA)聚合原位包埋制备了磁性SiO2固定化漆酶(Fe3O4@SiO2-PDA-Lac)。结果显示纳米颗粒尺寸均匀,并且保持较高的饱和磁性。通过最优条件制备出的固定化漆酶在50℃放置6h后,活性保持在63%,而游离酶仅保留18%。将固定化酶用于催化降解4-氯酚(4-CP),探究了溶液pH、漆酶浓度和ABTS[2,2-联氮-二(3-乙基-苯并噻唑-6-磺酸铵)]对4-CP去除率的影响。固定化漆酶在反应最适pH时,4-CP去除率为84.3%,而游离酶仅为65.7%。当漆酶浓度为1.2U·ml-1时,反应8h后,4-CP去除率可达95%,而游离酶的4-CP去除率仅82%。ABTS可促进固定化漆酶降解4-CP,当体系中加入50μmol·L-1的ABTS,反应10min后,固定化酶对4-CP去除率可达99%。固定化漆酶在重复使用10次后,4-CP去除率仍可达67%。

关键词：磁性纳米颗粒漆酶固定化催化降解氯酚废水